(Linear) (Six-base) MAPSORT of: KIEE5593.seq Check: 6591 from: 1 to: 1260 KIEE5593.orf With 182 enzymes: SgfI * January 29, 2014 12:07 .. AccI GT'mk_AC Cuts at: 0 849 1260 Size: 849 411 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 578 1260 Size: 578 682 AfeI AGC'GCT Cuts at: 0 65 1260 Size: 65 1195 AflII C'TTAA_G Cuts at: 0 720 1260 Size: 720 540 AflIII A'CryG_T Cuts at: 0 752 1260 Size: 752 508 ApaI G_GGCC'C Cuts at: 0 25 1260 Size: 25 1235 ApaLI G'TGCA_C Cuts at: 0 620 1260 Size: 620 640 AvaI C'yCGr_G Cuts at: 0 17 486 1260 Size: 17 469 774 Fragments arranged by size: 774 469 17 BanI G'GyrC_C Cuts at: 0 132 252 325 406 710 826 1260 Size: 132 120 73 81 304 116 434 Fragments arranged by size: 434 304 132 120 116 81 73 BanII G_rGCy'C Cuts at: 0 25 635 1260 Size: 25 610 625 Fragments arranged by size: 625 610 25 BbeI G_GCGC'C Cuts at: 0 136 329 410 1260 Size: 136 193 81 850 Fragments arranged by size: 850 193 136 81 BbsI GAAGACnn'nnnn_ Cuts at: 0 86 1062 1260 Size: 86 976 198 Fragments arranged by size: 976 198 86 BbvCI CC'TCA_GC Cuts at: 0 373 445 1260 Size: 373 72 815 Fragments arranged by size: 815 373 72 BfrBI ATG'CAT Cuts at: 0 1155 1260 Size: 1155 105 BglI GCCn_nnn'nGGC Cuts at: 0 610 1260 Size: 610 650 Bme1580I G_kGCm'C Cuts at: 0 25 187 257 554 624 1260 Size: 25 162 70 297 70 636 Fragments arranged by size: 636 297 162 70 70 25 BmrI ACTGGGnnnn_n' Cuts at: 0 509 1260 Size: 509 751 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 814 1260 Size: 814 446 Bpu10I CC'TnA_GC Cuts at: 0 373 445 1260 Size: 373 72 815 Fragments arranged by size: 815 373 72 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 793 910 1260 Size: 793 117 350 Fragments arranged by size: 793 350 117 BsaI GGTCTCn'nnnn_ Cuts at: 0 90 1260 Size: 90 1170 BsaAI yAC'GTr Cuts at: 0 276 753 1260 Size: 276 477 507 Fragments arranged by size: 507 477 276 BsaHI Gr'CG_yC Cuts at: 0 42 133 326 407 1260 Size: 42 91 193 81 853 Fragments arranged by size: 853 193 91 81 42 BsaWI w'CCGG_w Cuts at: 0 575 975 1260 Size: 575 400 285 Fragments arranged by size: 575 400 285 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 78 96 114 117 1260 Size: 78 18 18 3 1143 Fragments arranged by size: 1143 78 18 18 3 BseYI C'CCAG_C Cuts at: 0 80 161 424 700 1260 Size: 80 81 263 276 560 Fragments arranged by size: 560 276 263 81 80 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 964 1260 Size: 964 296 BsiEI CG_ry'CG Cuts at: 0 343 1260 Size: 343 917 BsiHKAI G_wGCw'C Cuts at: 0 16 205 317 624 1260 Size: 16 189 112 307 636 Fragments arranged by size: 636 307 189 112 16 BsmBI CGTCTCn'nnnn_ Cuts at: 0 510 1260 Size: 510 750 Bsp1286I G_dGCh'C Cuts at: 0 16 25 187 205 257 317 554 624 Size: 16 9 162 18 52 60 237 70 Cuts at: 624 635 1260 Size: 11 625 Fragments arranged by size: 625 237 162 70 60 52 18 16 11 9 BspEI T'CCGG_A Cuts at: 0 975 1260 Size: 975 285 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 272 680 1260 Size: 272 408 580 Fragments arranged by size: 580 408 272 BsrBI CCG'CTC Cuts at: 0 567 1260 Size: 567 693 BsrDI GCAATG_nn' Cuts at: 0 501 1260 Size: 501 759 BsrFI r'CCGG_y Cuts at: 0 295 322 338 956 1260 Size: 295 27 16 618 304 Fragments arranged by size: 618 304 295 27 16 BssHII G'CGCG_C Cuts at: 0 214 399 1260 Size: 214 185 861 Fragments arranged by size: 861 214 185 BstEII G'GTnAC_C Cuts at: 0 915 1260 Size: 915 345 Bsu36I CC'TnA_GG Cuts at: 0 706 1260 Size: 706 554 BtgI C'CryG_G Cuts at: 0 257 676 1260 Size: 257 419 584 Fragments arranged by size: 584 419 257 BtsI GCAGTG_nn' Cuts at: 0 622 1260 Size: 622 638 DraIII CAC_nnn'GTG Cuts at: 0 549 1260 Size: 549 711 DrdI GACnn_nn'nnGTC Cuts at: 0 753 1260 Size: 753 507 EaeI y'GGCC_r Cuts at: 0 308 340 359 461 650 1260 Size: 308 32 19 102 189 610 Fragments arranged by size: 610 308 189 102 32 19 EagI C'GGCC_G Cuts at: 0 340 1260 Size: 340 920 EarI CTCTTCn'nnn_ Cuts at: 0 292 1260 Size: 292 968 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 138 147 367 625 1260 Size: 138 9 220 258 635 Fragments arranged by size: 635 258 220 138 9 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 578 814 1260 Size: 578 236 446 Fragments arranged by size: 578 446 236 EcoNI CCTnn'n_nnAGG Cuts at: 0 391 1260 Size: 391 869 EcoO109I rG'GnC_Cy Cuts at: 0 21 22 813 996 1260 Size: 21 1 791 183 264 Fragments arranged by size: 791 264 183 21 1 FspI TGC'GCA Cuts at: 0 280 1260 Size: 280 980 HaeII r_GCGC'y Cuts at: 0 67 136 329 410 1103 1260 Size: 67 69 193 81 693 157 Fragments arranged by size: 693 193 157 81 69 67 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1196 1228 1260 Size: 1196 32 32 Fragments arranged by size: 1196 32 32 HincII GTy'rAC Cuts at: 0 234 1260 Size: 234 1026 HpaI GTT'AAC Cuts at: 0 234 1260 Size: 234 1026 KasI G'GCGC_C Cuts at: 0 132 325 406 1260 Size: 132 193 81 854 Fragments arranged by size: 854 193 132 81 MscI TGG'CCA Cuts at: 0 652 1260 Size: 652 608 MslI CAynn'nnrTG Cuts at: 0 595 1260 Size: 595 665 MspA1I CmG'CkG Cuts at: 0 171 306 348 377 678 1260 Size: 171 135 42 29 301 582 Fragments arranged by size: 582 301 171 135 42 29 NaeI GCC'GGC Cuts at: 0 324 340 1260 Size: 324 16 920 Fragments arranged by size: 920 324 16 NarI GG'CG_CC Cuts at: 0 133 326 407 1260 Size: 133 193 81 853 Fragments arranged by size: 853 193 133 81 NgoMIV G'CCGG_C Cuts at: 0 322 338 1260 Size: 322 16 922 Fragments arranged by size: 922 322 16 NsiI A_TGCA'T Cuts at: 0 1157 1260 Size: 1157 103 PasI CC'CwG_GG Cuts at: 0 884 1260 Size: 884 376 PfoI T'CCnGG_A Cuts at: 0 869 1260 Size: 869 391 PpuMI rG'GwC_Cy Cuts at: 0 813 996 1260 Size: 813 183 264 Fragments arranged by size: 813 264 183 PshAI GACnn'nnGTC Cuts at: 0 41 470 1260 Size: 41 429 790 Fragments arranged by size: 790 429 41 PspOMI G'GGCC_C Cuts at: 0 21 1260 Size: 21 1239 PstI C_TGCA'G Cuts at: 0 629 1177 1260 Size: 629 548 83 Fragments arranged by size: 629 548 83 PvuII CAG'CTG Cuts at: 0 306 1260 Size: 306 954 SacII CC_GC'GG Cuts at: 0 679 1260 Size: 679 581 SbfI CC_TGCA'GG Cuts at: 0 1177 1260 Size: 1177 83 SfcI C'TryA_G Cuts at: 0 625 1173 1260 Size: 625 548 87 Fragments arranged by size: 625 548 87 SfoI GGC'GCC Cuts at: 0 134 327 408 1260 Size: 134 193 81 852 Fragments arranged by size: 852 193 134 81 SmaI CCC'GGG Cuts at: 0 19 1260 Size: 19 1241 SmlI C'TyrA_G Cuts at: 0 720 808 925 1260 Size: 720 88 117 335 Fragments arranged by size: 720 335 117 88 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 262 1260 Size: 262 998 XbaI T'CTAG_A Cuts at: 0 777 1260 Size: 777 483 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 522 1260 Size: 522 738 XmaI C'CCGG_G Cuts at: 0 17 1260 Size: 17 1243 Enzymes that do cut: AccI AcuI AfeI AflII AflIII ApaI ApaLI AvaI BanI BanII BbeI BbsI BbvCI BfrBI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII BstEII Bsu36I BtgI BtsI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII HpaI KasI MscI MslI MspA1I NaeI NarI NgoMIV NsiI PasI PfoI PpuMI PshAI PspOMI PstI PvuII SacII SbfI SfcI SfoI SmaI SmlI TaqII XbaI XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AgeI AhdI AleI AlfI AloI AlwNI ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI BglII BlpI BmgBI BmtI BplI BsaBI BsaXI BsiWI BsmI BspHI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgZI ClaI CspCI DraI EcoICRI EcoRI EcoRV FalI FseI FspAI HindIII KpnI MfeI MluI MmeI NcoI NdeI NheI NotI NruI NspI PacI PciI PflMI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TatI TstI Tth111I XhoI XmnI ZraI