(Circular) (Six-base) MAPSORT of: pFN21AE2311.seq Check: 6911 from: 1 to: 10168 pFN21AE2311 10168 bp With 182 enzymes: SgfI * January 30, 2014 17:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7978 278 Size: 53 83 186 936 6442 2468 Fragments arranged by size: 6442 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 7511 1937 Size: 5574 4594 Acc65I G'GTAC_C Cuts at: 5405 5474 7489 5405 Size: 69 2015 8084 Fragments arranged by size: 8084 2015 69 AclI AA'CG_TT Cuts at: 1563 8295 8668 1563 Size: 6732 373 3063 Fragments arranged by size: 6732 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2777 4443 5203 5415 6561 7374 8231 9615 Size: 1145 1666 760 212 1146 813 857 1384 Cuts at: 9615 9883 1632 Size: 268 1917 Fragments arranged by size: 1917 1666 1384 1146 1145 857 813 760 268 212 AflII C'TTAA_G Cuts at: 820 1017 2665 820 Size: 197 1648 8323 Fragments arranged by size: 8323 1648 197 AflIII A'CryG_T Cuts at: 1203 9067 10044 1203 Size: 7864 977 1327 Fragments arranged by size: 7864 1327 977 AgeI A'CCGG_T Cuts at: 8999 8999 Size: 10168 AhdI GACnn_n'nnGTC Cuts at: 8897 8897 Size: 10168 AleI CACnn'nnGTG Cuts at: 1166 2836 5600 1166 Size: 1670 2764 5734 Fragments arranged by size: 5734 2764 1670 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 6242 1184 Size: 5058 5110 AlwNI CAG_nnn'CTG Cuts at: 2107 2191 7911 9483 2107 Size: 84 5720 1572 2792 Fragments arranged by size: 5720 2792 1572 84 ApaLI G'TGCA_C Cuts at: 2775 8225 9381 2775 Size: 5450 1156 3562 Fragments arranged by size: 5450 3562 1156 ApoI r'AATT_y Cuts at: 1493 2271 2538 2942 3358 5753 6643 7477 7703 Size: 778 267 404 416 2395 890 834 226 Cuts at: 7703 7855 9964 1493 Size: 152 2109 1697 Fragments arranged by size: 2395 2109 1697 890 834 778 416 404 267 226 152 AseI AT'TA_AT Cuts at: 160 1032 4587 8722 160 Size: 872 3555 4135 1606 Fragments arranged by size: 4135 3555 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10168 AvaI C'yCGr_G Cuts at: 1870 1943 4181 7493 1870 Size: 73 2238 3312 4545 Fragments arranged by size: 4545 3312 2238 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5270 5303 5397 5430 5270 Size: 33 94 33 10008 Fragments arranged by size: 10008 94 33 33 BamHI G'GATC_C Cuts at: 7498 7865 7498 Size: 367 9801 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5405 5474 7489 8944 618 Size: 325 217 627 3618 69 2015 1455 1842 Fragments arranged by size: 3618 2015 1842 1455 627 325 217 69 BanII G_rGCy'C Cuts at: 1391 2049 5944 7487 1391 Size: 658 3895 1543 4072 Fragments arranged by size: 4072 3895 1543 658 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2323 2404 2756 7172 7389 928 Size: 971 424 81 352 4416 217 3707 Fragments arranged by size: 4416 3707 971 424 352 217 81 BbvCI CC'TCA_GC Cuts at: 5548 5548 Size: 10168 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4460 4494 8359 8393 1903 Size: 34 2523 34 3865 34 3678 Fragments arranged by size: 3865 3678 2523 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2440 3125 8060 9270 2440 Size: 685 4935 1210 3338 Fragments arranged by size: 4935 3338 1210 685 BclI T'GATC_A Cuts at: 1548 2221 1548 Size: 673 9495 BfrBI ATG'CAT Cuts at: 2530 6611 2530 Size: 4081 6087 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 8779 136 Size: 107 122 71 631 605 7107 1525 Fragments arranged by size: 7107 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4400 6040 7186 10163 4400 Size: 1640 1146 2977 4405 Fragments arranged by size: 4405 2977 1640 1146 BlpI GC'TnA_GC Cuts at: 7585 7585 Size: 10168 Bme1580I G_kGCm'C Cuts at: 1790 2779 8229 9385 1790 Size: 989 5450 1156 2573 Fragments arranged by size: 5450 2573 1156 989 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 10168 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2880 5938 7899 Size: 416 53 83 399 24 1463 3058 1961 Cuts at: 7899 8857 442 Size: 958 1753 Fragments arranged by size: 3058 1961 1753 1463 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10168 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7366 7366 Size: 10168 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2310 2985 4534 8828 1239 Size: 1071 675 1549 4294 2579 Fragments arranged by size: 4294 2579 1549 1071 675 Bpu10I CC'TnA_GC Cuts at: 2100 5548 2100 Size: 3448 6720 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2507 5185 5303 8293 9158 9456 2507 Size: 2678 118 2990 865 298 3219 Fragments arranged by size: 3219 2990 2678 865 298 118 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 8831 882 Size: 617 7332 2219 Fragments arranged by size: 7332 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3173 5933 7959 493 Size: 711 1969 2760 2026 2702 Fragments arranged by size: 2760 2702 2026 1969 711 BsaBI GATnn'nnATC Cuts at: 2860 7185 10162 2860 Size: 4325 2977 2866 Fragments arranged by size: 4325 2977 2866 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6794 7975 8357 8992 Size: 53 83 186 936 5261 1181 382 635 Cuts at: 8992 275 Size: 1451 Fragments arranged by size: 5261 1451 1181 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 8601 8999 9273 9420 9831 8601 Size: 398 274 147 411 8938 Fragments arranged by size: 8938 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 9943 Fragments arranged by size: 9943 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3513 3860 7373 1186 Size: 2327 347 3513 3981 Fragments arranged by size: 3981 3513 2327 347 BseYI C'CCAG_C Cuts at: 2435 5322 6119 6405 9371 9867 2435 Size: 2887 797 286 2966 496 2736 Fragments arranged by size: 2966 2887 2736 797 496 286 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2860 5455 1163 Size: 573 1124 2595 5876 Fragments arranged by size: 5876 2595 1124 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2042 7618 8379 9407 1540 Size: 275 186 41 5576 761 1028 2301 Fragments arranged by size: 5576 2301 1028 761 275 186 41 BsiHKAI G_wGCw'C Cuts at: 2049 2779 8229 8314 9385 2049 Size: 730 5450 85 1071 2832 Fragments arranged by size: 5450 2832 1071 730 85 BsmI GAATG_Cn' Cuts at: 6434 7679 7772 6434 Size: 1245 93 8830 Fragments arranged by size: 8830 1245 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3967 3967 Size: 10168 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2779 5944 7487 8229 8314 9385 Size: 399 259 730 3165 1543 742 85 1071 Cuts at: 9385 1391 Size: 2174 Fragments arranged by size: 3165 2174 1543 1071 742 730 399 259 85 BspEI T'CCGG_A Cuts at: 9831 9831 Size: 10168 BspHI T'CATG_A Cuts at: 3397 3463 4333 8057 9787 3397 Size: 66 870 3724 1730 3778 Fragments arranged by size: 3778 3724 1730 870 66 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2944 3980 7523 844 Size: 2100 1036 3543 3489 Fragments arranged by size: 3543 3489 2100 1036 BsrBI CCG'CTC Cuts at: 1394 1823 4144 8055 1394 Size: 429 2321 3911 3507 Fragments arranged by size: 3911 3507 2321 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2439 8663 8837 65 Size: 1183 1191 6224 174 1396 Fragments arranged by size: 6224 1396 1191 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6034 8812 8999 1700 Size: 110 4224 2778 187 2869 Fragments arranged by size: 4224 2869 2778 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 10168 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10168 BssSI C'ACGA_G Cuts at: 8228 9240 8228 Size: 1012 9156 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 10168 BstBI TT'CG_AA Cuts at: 2767 6517 7380 8972 2767 Size: 3750 863 1592 3963 Fragments arranged by size: 3963 3750 1592 863 BstEII G'GTnAC_C Cuts at: 1184 3788 5210 1184 Size: 2604 1422 6142 Fragments arranged by size: 6142 2604 1422 BstXI CCAn_nnnn'nTGG Cuts at: 3545 4836 3545 Size: 1291 8877 BstYI r'GATC_y Cuts at: 1972 2369 3487 4147 4348 4400 5083 5569 5617 Size: 397 1118 660 201 52 683 486 48 Cuts at: 5617 6040 6393 6544 7186 7498 7865 8250 8267 Size: 423 353 151 642 312 367 385 17 Cuts at: 8267 9719 9805 9817 10163 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1118 683 660 642 486 423 397 385 367 353 346 312 201 151 86 52 48 17 12 Bsu36I CC'TnA_GG Cuts at: 3880 3880 Size: 10168 BtgI C'CryG_G Cuts at: 513 1065 4979 5565 6883 513 Size: 552 3914 586 1318 3798 Fragments arranged by size: 3914 3798 1318 586 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2044 5212 487 Size: 683 56 818 3168 5443 Fragments arranged by size: 5443 3168 818 683 56 BtsI GCAGTG_nn' Cuts at: 2514 2848 7684 8478 8498 2514 Size: 334 4836 794 20 4184 Fragments arranged by size: 4836 4184 794 334 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10168 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10133 DraI TTT'AAA Cuts at: 3187 7472 3187 Size: 4285 5883 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4258 9175 809 Size: 507 2942 4917 1802 Fragments arranged by size: 4917 2942 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2121 3692 3826 Size: 54 1425 50 275 18 291 1571 134 Cuts at: 3826 6880 7615 8504 10133 8 Size: 3054 735 889 1629 43 Fragments arranged by size: 3054 1629 1571 1425 889 735 291 275 134 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7615 1537 Size: 275 5803 4090 Fragments arranged by size: 5803 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2262 3187 3197 4260 4407 4428 4503 5280 Size: 909 925 10 1063 147 21 75 777 Cuts at: 5280 5856 6294 6905 7260 7350 8098 1353 Size: 576 438 611 355 90 748 3423 Fragments arranged by size: 3423 1063 925 909 777 748 611 576 438 355 147 90 75 21 10 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5124 8755 9129 9275 387 Size: 1064 138 3535 3631 374 146 1280 Fragments arranged by size: 3631 3535 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2047 2047 Size: 10168 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2310 2777 2985 4443 4534 5203 5415 Size: 393 678 467 208 1458 91 669 212 Cuts at: 5415 6561 7374 8231 8828 9615 9883 1239 Size: 1146 813 857 597 787 268 1524 Fragments arranged by size: 1524 1458 1146 857 813 787 678 669 597 467 393 268 212 208 91 EcoO109I rG'GnC_Cy Cuts at: 2178 2252 6917 2178 Size: 74 4665 5429 Fragments arranged by size: 5429 4665 74 EcoRI G'AATT_C Cuts at: 2538 3358 6643 7477 7855 2538 Size: 820 3285 834 378 4851 Fragments arranged by size: 4851 3285 834 820 378 EcoRV GAT'ATC Cuts at: 2858 3809 2858 Size: 951 9217 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2340 7347 2340 Size: 5007 5161 FspI TGC'GCA Cuts at: 8674 8674 Size: 10168 HaeII r_GCGC'y Cuts at: 1678 2956 9315 1678 Size: 1278 6359 2531 Fragments arranged by size: 6359 2531 1278 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2024 2031 2056 2063 3062 Size: 32 135 32 452 7 25 7 999 Cuts at: 3062 3094 3649 3681 5423 5430 5455 5462 5531 Size: 32 555 32 1742 7 25 7 69 Cuts at: 5531 5563 6266 6298 6551 6583 7231 7263 7407 Size: 32 703 32 253 32 648 32 144 Cuts at: 7407 7439 8809 8841 8883 8915 1373 Size: 32 1370 32 42 32 2626 Fragments arranged by size: 2626 1742 1370 999 703 648 555 452 253 144 135 69 42 32 32 32 32 32 32 32 32 32 32 32 25 25 7 7 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 2419 3109 3374 7434 7512 7758 Size: 988 281 481 690 265 4060 78 246 Cuts at: 7758 669 Size: 3079 Fragments arranged by size: 4060 3079 988 690 481 281 265 246 78 HindIII A'AGCT_T Cuts at: 748 6663 748 Size: 5915 4253 HpaI GTT'AAC Cuts at: 2419 7758 2419 Size: 5339 4829 KpnI G_GTAC'C Cuts at: 5409 5478 7493 5409 Size: 69 2015 8084 Fragments arranged by size: 8084 2015 69 MfeI C'AATT_G Cuts at: 7767 7767 Size: 10168 MluI A'CGCG_T Cuts at: 10044 10044 Size: 10168 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2525 3150 3195 4930 5636 9282 9466 1305 Size: 1220 625 45 1735 706 3646 184 2007 Fragments arranged by size: 3646 2007 1735 1220 706 625 184 45 MscI TGG'CCA Cuts at: 10 64 1489 1832 6882 10 Size: 54 1425 343 5050 3296 Fragments arranged by size: 5050 3296 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2525 2836 3275 3402 4332 4527 4834 Size: 648 1359 311 439 127 930 195 307 Cuts at: 4834 5600 6083 8126 8485 8644 518 Size: 766 483 2043 359 159 2042 Fragments arranged by size: 2043 2042 1359 930 766 648 483 439 359 311 307 195 159 127 MspA1I CmG'CkG Cuts at: 1606 2729 4193 6581 6818 7372 7584 8261 9409 Size: 1123 1464 2388 237 554 212 677 1148 Cuts at: 9409 9654 1606 Size: 245 2120 Fragments arranged by size: 2388 2120 1464 1148 1123 677 554 245 237 212 NcoI C'CATG_G Cuts at: 513 1065 4979 6883 513 Size: 552 3914 1904 3798 Fragments arranged by size: 3914 3798 1904 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 10168 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10168 NotI GC'GGCC_GC Cuts at: 7615 7615 Size: 10168 NsiI A_TGCA'T Cuts at: 2532 6613 2532 Size: 4081 6087 NspI r_CATG'y Cuts at: 1132 1229 2357 2678 3597 7080 7526 9071 1132 Size: 97 1128 321 919 3483 446 1545 2229 Fragments arranged by size: 3483 2229 1545 1128 919 446 321 97 PasI CC'CwG_GG Cuts at: 1348 5484 1348 Size: 4136 6032 PciI A'CATG_T Cuts at: 9067 9067 Size: 10168 PflMI CCAn_nnn'nTGG Cuts at: 1108 2191 1108 Size: 1083 9085 PfoI T'CCnGG_A Cuts at: 2070 2287 4344 2070 Size: 217 2057 7894 Fragments arranged by size: 7894 2057 217 PmeI GTTT'AAAC Cuts at: 7472 7472 Size: 10168 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5878 5910 8237 8269 9750 9782 5878 Size: 32 2327 32 1481 32 6264 Fragments arranged by size: 6264 2327 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2178 2252 6917 2178 Size: 74 4665 5429 Fragments arranged by size: 5429 4665 74 PshAI GACnn'nnGTC Cuts at: 1613 2551 1613 Size: 938 9230 PsiI TTA'TAA Cuts at: 3323 5783 5802 5820 6279 7738 3323 Size: 2460 19 18 459 1459 5753 Fragments arranged by size: 5753 2460 1459 459 19 18 PstI C_TGCA'G Cuts at: 830 2509 3331 4596 7520 830 Size: 1679 822 1265 2924 3478 Fragments arranged by size: 3478 2924 1679 1265 822 PvuI CG_AT'CG Cuts at: 2001 2042 2001 Size: 41 10127 PvuII CAG'CTG Cuts at: 2729 4193 6581 6818 7372 2729 Size: 1464 2388 237 554 5525 Fragments arranged by size: 5525 2388 1464 554 237 SacI G_AGCT'C Cuts at: 2049 2049 Size: 10168 SalI G'TCGA_C Cuts at: 1936 7510 1936 Size: 5574 4594 SapI GCTCTTCn'nnn_ Cuts at: 3197 6294 3197 Size: 3097 7071 SbfI CC_TGCA'GG Cuts at: 7520 7520 Size: 10168 ScaI AGT'ACT Cuts at: 7062 8416 10128 7062 Size: 1354 1712 7102 Fragments arranged by size: 7102 1712 1354 SfcI C'TryA_G Cuts at: 826 1045 2505 2822 3327 4592 6061 7001 7251 Size: 219 1460 317 505 1265 1469 940 250 Cuts at: 7251 7516 8651 9332 9523 826 Size: 265 1135 681 191 1471 Fragments arranged by size: 1471 1469 1460 1265 1135 940 681 505 317 265 250 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10168 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10168 SmaI CCC'GGG Cuts at: 1872 7495 1872 Size: 5623 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2486 2665 5164 5282 8272 9173 Size: 197 926 543 179 2499 118 2990 901 Cuts at: 9173 9435 820 Size: 262 1553 Fragments arranged by size: 2990 2499 1553 926 901 543 262 197 179 118 SnaBI TAC'GTA Cuts at: 493 5933 7959 493 Size: 5440 2026 2702 Fragments arranged by size: 5440 2702 2026 SpeI A'CTAG_T Cuts at: 152 3131 152 Size: 2979 7189 SphI G_CATG'C Cuts at: 1132 3597 7526 1132 Size: 2465 3929 3774 Fragments arranged by size: 3929 3774 2465 SspI AAT'ATT Cuts at: 5 52 8092 5 Size: 47 8040 2081 Fragments arranged by size: 8040 2081 47 StuI AGG'CCT Cuts at: 1517 2025 1517 Size: 508 9660 StyI C'CwwG_G Cuts at: 513 1065 3538 4979 5354 6883 7607 513 Size: 552 2473 1441 375 1529 724 3074 Fragments arranged by size: 3074 2473 1529 1441 724 552 375 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2449 4874 8195 8212 8365 8550 8995 Size: 1255 869 2425 3321 17 153 185 445 Cuts at: 8995 325 Size: 1498 Fragments arranged by size: 3321 2425 1498 1255 869 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2293 2562 5624 Size: 274 80 33 51 1443 316 269 3062 Cuts at: 5624 6781 7060 8414 10126 96 Size: 1157 279 1354 1712 138 Fragments arranged by size: 3062 1712 1443 1354 1157 316 279 274 269 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5194 5226 10128 10160 5194 Size: 32 4902 32 5202 Fragments arranged by size: 5202 4902 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2085 1316 Size: 769 9399 XbaI T'CTAG_A Cuts at: 5080 7504 5080 Size: 2424 7744 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10168 XmaI C'CCGG_G Cuts at: 1870 7493 1870 Size: 5623 4545 XmnI GAAnn'nnTTC Cuts at: 5274 7062 8297 5274 Size: 1788 1235 7145 Fragments arranged by size: 7145 1788 1235 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7976 276 Size: 53 83 186 936 6442 2468 Fragments arranged by size: 6442 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BsiWI BstZ17I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI