Restriction map for
pFN21AB6450
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI ApaI ApaLI
AscI Asp718I AspEI AspHI AspI
AviII AvrII BalI BbeI BbrPI
BbvCI BbvI BcgI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DrdI EaeI EagI Eam1105I EclXI
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT14I FauI FbaI Fnu4HI
FseI FspI Hin1I HincII HindII
HpaI ItaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI StuI
StyI SwaI TseI Tsp45I Tth111I
XbaI XcmI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AciI 997 CCGC CCGC(-3/-1)
AfaI 190 GTAC GT^AC
Aor51HI 693 AGCGCT AGC^GCT
AseI 399 ATTAAT AT^TAAT
AsnI 399 ATTAAT AT^TAAT
Asp700I 97 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 244 GGACC G^G[AT]CC
BamHI 51 GGATCC G^GATCC
BanII 737 GAGCCC G[GA]GC[CT]^C
BbsI 127 GAAGAC GAAGAC(2/6)
BfaI 980 CTAG C^TAG
BglI 739 GCCCGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 782 CTCCAG CTGGAG(16/14)
BpuAI 127 GAAGAC GAAGAC(2/6)
BsmAI 856 GAGAC GTCTC(1/5)
BsmFI 724 GTCCC GGGAC(10/14)
BsrI 700 CCAGT ACTGG(1/-1)
BstF5I 933 GGATG GGATG(2/0)
BstXI 955 CCAGAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 51 GGATCC [GA]^GATC[CT]
Cac8I 66 GCATGC GC[ACGT]^[ACGT]GC
DraIII 887 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 693 AGCGCT AGC^GCT
EcoT22I 449 ATGCAT ATGCA^T
FokI 933 GGATG GGATG(9/13)
HaeII 693 AGCGCT [GA]GCGC^[CT]
HapII 741 CCGG C^CGG
HgaI 70 GCGTC GACGC(5/10)
HpaII 741 CCGG C^CGG
MaeI 980 CTAG C^TAG
MaeIII 682 GTAAC ^GT[ACGT]AC
MflI 51 GGATCC [GA]^GATC[CT]
MspI 741 CCGG C^CGG
NsiI 449 ATGCAT ATGCA^T
NspI 66 GCATGC [GA]CATG^[CT]
PaeR7I 734 CTCGAG C^TCGAG
PflMI 640 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 399 ATTAAT AT^TAAT
RsaI 190 GTAC GT^AC
ScaI 189 AGTACT AGT^ACT
SfaNI 934 GATGC GCATC(5/9)
SmaI 740 CCCGGG CCC^GGG
SphI 66 GCATGC GCATG^C
SspI 14 AATATT AAT^ATT
TaqI 735 TCGA T^CGA
TfiI 195 GATTC G^A[AT]TC
TthHB8I 735 TCGA T^CGA
Van91I 640 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 734 CTCGAG C^TCGAG
XhoII 51 GGATCC [GA]^GATC[CT]
XmaCI 740 CCCGGG C^CCGGG
XmaI 740 CCCGGG C^CCGGG
XmnI 97 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 74 CGCG CG^CG
876 CGCG
AcsI 469 AAATTC [GA]^AATT[CT]
497 AAATTC
ApoI 469 AAATTC [GA]^AATT[CT]
497 AAATTC
BcnI 740 CCCGG CC^[GC]GG
741 CCGGG
BsaAI 437 CACGTA [CT]AC^GT[GA]
515 TACGTG
BstUI 74 CGCG CG^CG
876 CGCG
CfoI 75 GCGC GCG^C
694 GCGC
DsaI 615 CCGTGG C^C[GA][CT]GG
687 CCGTGG
EcoNI 260 CCTTGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
646 CCTGGACGAGG
HaeIII 613 GGCC GG^CC
966 GGCC
HhaI 75 GCGC GCG^C
694 GCGC
HinP1I 75 GCGC G^CGC
694 GCGC
HindIII 428 AAGCTT A^AGCTT
794 AAGCTT
HphI 832 GGTGA GGTGA(8/7)
662 TCACC
MaeII 438 ACGT A^CGT
516 ACGT
MslI 376 CACTTGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1030 CACCCCTATG
MvnI 74 CGCG CG^CG
876 CGCG
NciI 740 CCCGG CC^[GC]GG
741 CCGGG
PleI 547 GACTC GAGTC(4/5)
444 GAGTC
PshAI 547 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
910 GACTACGGTC
SfcI 271 CTACAG C^T[GA][CT]AG
710 CTGTAG
SmlI 378 CTTGAG C^T[CT][GA]AG
734 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AluI AlwI AvaI BanI BciVI
BmyI BsaJI BseRI BsiYI BslI
BsoBI Bsp1286I BssKI BstNI Cfr13I
DdeI DpnII EarI EcoRII HinfI
Ksp632I MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI Tru9I Tsp509I
TspRI