(Circular) (Six-base) MAPSORT of: pFN21AE6457.seq Check: 4201 from: 1 to: 6133 pFN21AE6457 6133 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3943 278 Size: 53 83 186 936 2407 2468 Fragments arranged by size: 2468 2407 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3476 1937 Size: 1539 4594 Acc65I G'GTAC_C Cuts at: 2886 3454 2886 Size: 568 5565 AclI AA'CG_TT Cuts at: 1563 4260 4633 1563 Size: 2697 373 3063 Fragments arranged by size: 3063 2697 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3212 4196 5580 5848 1632 Size: 1580 984 1384 268 1917 Fragments arranged by size: 1917 1580 1384 984 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5936 AflIII A'CryG_T Cuts at: 1203 3184 5032 6009 1203 Size: 1981 1848 977 1327 Fragments arranged by size: 1981 1848 1327 977 AgeI A'CCGG_T Cuts at: 4964 4964 Size: 6133 AhdI GACnn_n'nnGTC Cuts at: 2299 4862 2299 Size: 2563 3570 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6133 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6133 AlwNI CAG_nnn'CTG Cuts at: 3876 5448 3876 Size: 1572 4561 ApaLI G'TGCA_C Cuts at: 4190 5346 4190 Size: 1156 4977 ApoI r'AATT_y Cuts at: 1493 2179 2258 2275 2570 2818 3293 3442 3668 Size: 686 79 17 295 248 475 149 226 Cuts at: 3668 3820 5929 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 686 475 295 248 226 152 149 79 17 AseI AT'TA_AT Cuts at: 160 1032 2483 4687 160 Size: 872 1451 2204 1606 Fragments arranged by size: 2204 1606 1451 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 AvaI C'yCGr_G Cuts at: 1870 1943 3458 1870 Size: 73 1515 4545 Fragments arranged by size: 4545 1515 73 BamHI G'GATC_C Cuts at: 3463 3830 3463 Size: 367 5766 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2886 3454 4909 618 Size: 325 217 627 1099 568 1455 1842 Fragments arranged by size: 1842 1455 1099 627 568 325 217 BanII G_rGCy'C Cuts at: 1391 3452 1391 Size: 2061 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2202 928 Size: 971 303 4859 Fragments arranged by size: 4859 971 303 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2120 2154 4324 4358 1903 Size: 34 183 34 2170 34 3678 Fragments arranged by size: 3678 2170 183 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2361 2920 4025 5235 2361 Size: 559 1105 1210 3259 Fragments arranged by size: 3259 1210 1105 559 BclI T'GATC_A Cuts at: 1548 1548 Size: 6133 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4744 136 Size: 107 122 71 631 605 3072 1525 Fragments arranged by size: 3072 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2022 6128 2022 Size: 4106 2027 BlpI GC'TnA_GC Cuts at: 3550 3550 Size: 6133 Bme1580I G_kGCm'C Cuts at: 1790 3263 4194 5350 1790 Size: 1473 931 1156 2573 Fragments arranged by size: 2573 1473 1156 931 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6133 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3864 4822 442 Size: 416 53 83 399 24 2447 958 1753 Fragments arranged by size: 2447 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6133 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4793 1239 Size: 3554 2579 Bpu10I CC'TnA_GC Cuts at: 2431 2609 2431 Size: 178 5955 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4258 5123 5421 4258 Size: 865 298 4970 Fragments arranged by size: 4970 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4796 882 Size: 617 3297 2219 Fragments arranged by size: 3297 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3924 493 Size: 711 2720 2702 Fragments arranged by size: 2720 2702 711 BsaBI GATnn'nnATC Cuts at: 2027 6127 2027 Size: 4100 2033 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3940 4322 4957 275 Size: 53 83 186 936 2407 382 635 1451 Fragments arranged by size: 2407 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2012 4566 4964 5238 5385 5796 2012 Size: 2554 398 274 147 411 2349 Fragments arranged by size: 2554 2349 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2054 2084 2402 2432 1178 Size: 30 165 30 651 30 318 30 4879 Fragments arranged by size: 4879 651 318 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2322 3051 3285 3375 1186 Size: 1136 729 234 90 3944 Fragments arranged by size: 3944 1136 729 234 90 BseYI C'CCAG_C Cuts at: 2454 5336 5832 2454 Size: 2882 496 2755 Fragments arranged by size: 2882 2755 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2407 1163 Size: 573 671 4889 Fragments arranged by size: 4889 671 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3583 4344 5372 1540 Size: 275 186 1582 761 1028 2301 Fragments arranged by size: 2301 1582 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3277 4194 4279 5350 3277 Size: 917 85 1071 4060 Fragments arranged by size: 4060 1071 917 85 BsmI GAATG_Cn' Cuts at: 2242 3644 3737 2242 Size: 1402 93 4638 Fragments arranged by size: 4638 1402 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3263 3277 3452 4194 4279 5350 1391 Size: 399 1473 14 175 742 85 1071 2174 Fragments arranged by size: 2174 1473 1071 742 399 175 85 14 BspEI T'CCGG_A Cuts at: 5796 5796 Size: 6133 BspHI T'CATG_A Cuts at: 4022 5752 4022 Size: 1730 4403 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2647 2967 3488 844 Size: 1803 320 521 3489 Fragments arranged by size: 3489 1803 521 320 BsrBI CCG'CTC Cuts at: 1394 1823 4020 1394 Size: 429 2197 3507 Fragments arranged by size: 3507 2197 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2447 4628 4802 65 Size: 1183 1199 2181 174 1396 Fragments arranged by size: 2181 1396 1199 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4777 4964 1700 Size: 110 2967 187 2869 Fragments arranged by size: 2967 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6133 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6133 BssSI C'ACGA_G Cuts at: 4193 5205 4193 Size: 1012 5121 BstAPI GCAn_nnn'nTGC Cuts at: 794 2421 794 Size: 1627 4506 BstBI TT'CG_AA Cuts at: 4937 4937 Size: 6133 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6133 BstYI r'GATC_y Cuts at: 1972 2022 3045 3463 3830 4215 4232 5684 5770 Size: 50 1023 418 367 385 17 1452 86 Cuts at: 5770 5782 6128 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1023 418 385 367 346 86 50 17 12 BtgI C'CryG_G Cuts at: 513 1065 2967 513 Size: 552 1902 3679 Fragments arranged by size: 3679 1902 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5394 Fragments arranged by size: 5394 683 56 BtsI GCAGTG_nn' Cuts at: 2745 3649 4443 4463 2745 Size: 904 794 20 4415 Fragments arranged by size: 4415 904 794 20 ClaI AT'CG_AT Cuts at: 1554 2871 1554 Size: 1317 4816 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6098 DraI TTT'AAA Cuts at: 2569 3437 2569 Size: 868 5265 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5140 809 Size: 507 3824 1802 Fragments arranged by size: 3824 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2799 3580 4469 Size: 54 1425 50 275 18 969 781 889 Cuts at: 4469 6098 8 Size: 1629 43 Fragments arranged by size: 1629 1425 969 889 781 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3580 1537 Size: 275 1768 4090 Fragments arranged by size: 4090 1768 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2310 2382 3082 4063 1353 Size: 957 72 700 981 3423 Fragments arranged by size: 3423 981 957 700 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4720 5094 5240 387 Size: 1064 138 3131 374 146 1280 Fragments arranged by size: 3131 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3212 4196 4793 5580 5848 1239 Size: 393 1580 984 597 787 268 1524 Fragments arranged by size: 1580 1524 984 787 597 393 268 EcoRI G'AATT_C Cuts at: 3442 3820 3442 Size: 378 5755 FspI TGC'GCA Cuts at: 4639 4639 Size: 6133 HaeII r_GCGC'y Cuts at: 1678 5280 1678 Size: 3602 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2007 2039 2285 2317 4774 Size: 32 135 32 435 32 246 32 2457 Cuts at: 4774 4806 4848 4880 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2457 435 246 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2378 3477 3723 669 Size: 988 281 440 1099 246 3079 Fragments arranged by size: 3079 1099 988 440 281 246 HindIII A'AGCT_T Cuts at: 748 3224 748 Size: 2476 3657 HpaI GTT'AAC Cuts at: 3723 3723 Size: 6133 KpnI G_GTAC'C Cuts at: 2890 3458 2890 Size: 568 5565 MfeI C'AATT_G Cuts at: 3732 3732 Size: 6133 MluI A'CGCG_T Cuts at: 6009 6009 Size: 6133 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3105 3399 5247 5431 1305 Size: 1800 294 1848 184 2007 Fragments arranged by size: 2007 1848 1800 294 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4311 Fragments arranged by size: 4311 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4091 4450 4609 518 Size: 648 2925 359 159 2042 Fragments arranged by size: 2925 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3549 4226 5374 5619 1606 Size: 1943 677 1148 245 2120 Fragments arranged by size: 2120 1943 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2967 513 Size: 552 1902 3679 Fragments arranged by size: 3679 1902 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6133 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6133 NotI GC'GGCC_GC Cuts at: 3580 3580 Size: 6133 NspI r_CATG'y Cuts at: 1132 1229 2430 2462 2922 3005 3188 3239 3491 Size: 97 1201 32 460 83 183 51 252 Cuts at: 3491 5036 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 1201 460 252 183 97 83 51 32 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6133 PciI A'CATG_T Cuts at: 3184 5032 3184 Size: 1848 4285 PflMI CCAn_nnn'nTGG Cuts at: 1108 3315 1108 Size: 2207 3926 PmeI GTTT'AAAC Cuts at: 3437 3437 Size: 6133 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4202 4234 5715 5747 4202 Size: 32 1481 32 4588 Fragments arranged by size: 4588 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6133 PsiI TTA'TAA Cuts at: 2679 3165 3703 2679 Size: 486 538 5109 Fragments arranged by size: 5109 538 486 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2454 2486 2454 Size: 32 6101 PstI C_TGCA'G Cuts at: 830 3485 830 Size: 2655 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6133 SalI G'TCGA_C Cuts at: 1936 3475 1936 Size: 1539 4594 SbfI CC_TGCA'GG Cuts at: 3485 3485 Size: 6133 ScaI AGT'ACT Cuts at: 4381 6093 4381 Size: 1712 4421 SfcI C'TryA_G Cuts at: 826 1045 2046 2173 3481 4616 5297 5488 826 Size: 219 1001 127 1308 1135 681 191 1471 Fragments arranged by size: 1471 1308 1135 1001 681 219 191 127 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6133 SmaI CCC'GGG Cuts at: 1872 3460 1872 Size: 1588 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4237 5138 5400 820 Size: 197 926 2294 901 262 1553 Fragments arranged by size: 2294 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3924 493 Size: 3431 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6133 SphI G_CATG'C Cuts at: 1132 2922 3005 3491 1132 Size: 1790 83 486 3774 Fragments arranged by size: 3774 1790 486 83 SspI AAT'ATT Cuts at: 5 52 4057 5 Size: 47 4005 2081 Fragments arranged by size: 4005 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6133 StyI C'CwwG_G Cuts at: 513 1065 2161 2345 2578 2967 3572 513 Size: 552 1096 184 233 389 605 3074 Fragments arranged by size: 3074 1096 605 552 389 233 184 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2781 4160 4177 4330 4515 4960 325 Size: 1255 1201 1379 17 153 185 445 1498 Fragments arranged by size: 1498 1379 1255 1201 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4379 6091 96 Size: 274 80 33 51 1443 2402 1712 138 Fragments arranged by size: 2402 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6093 6125 6093 Size: 32 6101 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6133 XbaI T'CTAG_A Cuts at: 3469 3469 Size: 6133 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2752 2752 Size: 6133 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6133 XmaI C'CCGG_G Cuts at: 1870 3458 1870 Size: 1588 4545 XmnI GAAnn'nnTTC Cuts at: 4262 4262 Size: 6133 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3941 276 Size: 53 83 186 936 2407 2468 Fragments arranged by size: 2468 2407 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI