Restriction map for
pFN21AB7739
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
Asp700I Asp718I AspEI AvaI AvrII
BamHI BanII BbeI BbrPI BcgI
BciVI BfaI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsgI
BsiWI BsoBI Bsp1407I BspDI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
Eam1105I Eco47III Eco81I EcoO109I EcoRI
EcoRV EcoT14I EcoT22I FseI HaeII
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MluI
MslI NaeI NarI NcoI NdeI
NgoMIV NheI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
TaqI TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 859 GTCTAC GT^[AC][GT]AC
AccII 250 CGCG CG^CG
AccIII 853 TCCGGA T^CCGGA
AcyI 631 GGCGTC G[GA]^CG[CT]C
AfaI 1102 GTAC GT^AC
AflIII 1104 ACATGT A^C[GA][CT]GT
AlwI 1263 GGATC GGATC(4/5)
AseI 52 ATTAAT AT^TAAT
AsnI 52 ATTAAT AT^TAAT
AspHI 555 GAGCAC G[AT]GC[AT]^C
AspI 564 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 771 GGACC G^G[AT]CC
BanI 161 GGCACC G^G[CT][GA]CC
BmyI 555 GAGCAC G[AGT]GC[ACT]^C
BpmI 408 CTGGAG CTGGAG(16/14)
BsaHI 631 GGCGTC G[GA]^CG[CT]C
BsaWI 853 TCCGGA [AT]^CCGG[AT]
BseAI 853 TCCGGA T^CCGGA
BsiEI 246 CGGCCG CG[GA][CT]^CG
BsiHKAI 555 GAGCAC G[AT]GC[AT]^C
BsmBI 534 CGTCTC CGTCTC(1/5)
BsmI 150 GAATGC GAATGC(1/-1)
Bsp1286I 555 GAGCAC G[AGT]GC[ACT]^C
BspEI 853 TCCGGA T^CCGGA
BspLU11I 1104 ACATGT A^CATGT
BstEII 396 GGTCACC G^GT[ACGT]ACC
BstPI 396 GGTCACC G^GT[ACGT]ACC
BstUI 250 CGCG CG^CG
BstXI 636 CCAATTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 965 GCTGGC GC[ACGT]^[ACGT]GC
EagI 246 CGGCCG C^GGCCG
EclXI 246 CGGCCG C^GGCCG
Eco52I 246 CGGCCG C^GGCCG
EcoNI 370 CCTTGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 396 GGTCACC G^GT[ACGT]ACC
Hin1I 631 GGCGTC G[GA]^CG[CT]C
MfeI 637 CAATTG C^AATTG
MroI 853 TCCGGA T^CCGGA
MunI 637 CAATTG C^AATTG
MvnI 250 CGCG CG^CG
NotI 245 GCGGCCGC GC^GGCCGC
NspI 1104 ACATGT [GA]CATG^[CT]
PflFI 564 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 401 CCAACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 857 GAGTC GAGTC(4/5)
PshBI 52 ATTAAT AT^TAAT
PstI 1037 CTGCAG CTGCA^G
RsaI 1102 GTAC GT^AC
SapI 497 GAAGAGC GCTCTTC(1/4)
SexAI 565 ACCAGGT A^CC[AT]GGT
SfaNI 209 GCATC GCATC(5/9)
SspI 1199 AATATT AAT^ATT
Tth111I 564 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 401 CCAACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AviII 153 TGCGCA TGC^GCA
182 TGCGCA
BalI 106 TGGCCA TGG^CCA
1066 TGGCCA
BbsI 25 GAAGAC GAAGAC(2/6)
1156 GAAGAC
BbvCI 679 CCTCAGC CCTCAGC(-5/-2)
487 GCTGAGG
BclI 456 TGATCA T^GATCA
867 TGATCA
BcnI 311 CCCGG CC^[GC]GG
393 CCCGG
BglII 278 AGATCT A^GATCT
744 AGATCT
BpuAI 25 GAAGAC GAAGAC(2/6)
1156 GAAGAC
BsaJI 228 CCTGGG C^C[ACGT][ACGT]GG
375 CCGAGG
BseRI 677 CTCCTC GAGGAG(10/8)
265 GAGGAG
BsmFI 338 GTCCC GGGAC(10/14)
1055 GTCCC
BstYI 278 AGATCT [GA]^GATC[CT]
744 AGATCT
FauI 206 CCCGC CCCGC(4/6)
1078 GCGGG
FbaI 456 TGATCA T^GATCA
867 TGATCA
FspI 153 TGCGCA TGC^GCA
182 TGCGCA
HgaI 422 GACGC GACGC(5/10)
632 GCGTC
MaeII 77 ACGT A^CGT
1124 ACGT
MflI 278 AGATCT [GA]^GATC[CT]
744 AGATCT
MluNI 106 TGGCCA TGG^CCA
1066 TGGCCA
MscI 106 TGGCCA TGG^CCA
1066 TGGCCA
NciI 311 CCCGG CC^[GC]GG
393 CCCGG
PvuII 171 CAGCTG CAG^CTG
963 CAGCTG
SmlI 214 CTCAAG C^T[CT][GA]AG
1151 CTCAAG
XhoII 278 AGATCT [GA]^GATC[CT]
744 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI BbvI BsaI BsiYI
BslI BsmAI BspMI BsrI BssKI
BstF5I BstNI CfoI Cfr13I DdeI
DpnII EaeI EarI EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfcI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI