(Circular) (Six-base) MAPSORT of: pFN21AB7739.seq Check: 2432 from: 1 to: 6013 pFN21AB7739 6013 bp With 182 enzymes: SgfI * March 28, 2012 12:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3049 3049 Size: 6013 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3823 278 Size: 53 83 186 936 2287 2468 Fragments arranged by size: 2468 2287 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2865 3356 1937 Size: 928 491 4594 Fragments arranged by size: 4594 928 491 Acc65I G'GTAC_C Cuts at: 3334 3334 Size: 6013 AclI AA'CG_TT Cuts at: 1563 4140 4513 1563 Size: 2577 373 3063 Fragments arranged by size: 3063 2577 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2049 2384 2573 2988 4076 5460 5728 1632 Size: 417 335 189 415 1088 1384 268 1917 Fragments arranged by size: 1917 1384 1088 417 415 335 268 189 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5816 AflIII A'CryG_T Cuts at: 1203 3109 4912 5889 1203 Size: 1906 1803 977 1327 Fragments arranged by size: 1906 1803 1327 977 AgeI A'CCGG_T Cuts at: 4844 4844 Size: 6013 AhdI GACnn_n'nnGTC Cuts at: 4742 4742 Size: 6013 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6013 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6013 AlwNI CAG_nnn'CTG Cuts at: 3756 5328 3756 Size: 1572 4441 ApaLI G'TGCA_C Cuts at: 4070 5226 4070 Size: 1156 4857 ApoI r'AATT_y Cuts at: 1493 3322 3548 3700 5809 1493 Size: 1829 226 152 2109 1697 Fragments arranged by size: 2109 1829 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2058 4567 160 Size: 872 1026 2509 1606 Fragments arranged by size: 2509 1606 1026 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 AvaI C'yCGr_G Cuts at: 1870 1943 3338 1870 Size: 73 1395 4545 Fragments arranged by size: 4545 1395 73 BamHI G'GATC_C Cuts at: 3343 3710 3343 Size: 367 5646 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2166 3334 4789 618 Size: 325 217 627 379 1168 1455 1842 Fragments arranged by size: 1842 1455 1168 627 379 325 217 BanII G_rGCy'C Cuts at: 1391 3332 1391 Size: 1941 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2037 3168 928 Size: 971 138 1131 3773 Fragments arranged by size: 3773 1131 971 138 BbvCI CC'TCA_GC Cuts at: 2493 2685 2493 Size: 192 5821 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4204 4238 1903 Size: 34 2267 34 3678 Fragments arranged by size: 3678 2267 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3905 5115 3905 Size: 1210 4803 BclI T'GATC_A Cuts at: 1548 2461 2872 1548 Size: 913 411 4689 Fragments arranged by size: 4689 913 411 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4624 136 Size: 107 122 71 631 605 2952 1525 Fragments arranged by size: 2952 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2283 2749 6008 2283 Size: 466 3259 2288 Fragments arranged by size: 3259 2288 466 BlpI GC'TnA_GC Cuts at: 3430 3430 Size: 6013 Bme1580I G_kGCm'C Cuts at: 1790 4074 5230 1790 Size: 2284 1156 2573 Fragments arranged by size: 2573 2284 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6013 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3744 4702 442 Size: 416 53 83 399 24 2327 958 1753 Fragments arranged by size: 2327 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6013 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2434 4673 1239 Size: 1195 2239 2579 Fragments arranged by size: 2579 2239 1195 Bpu10I CC'TnA_GC Cuts at: 2171 2493 2685 2171 Size: 322 192 5499 Fragments arranged by size: 5499 322 192 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2204 3141 4138 5003 5301 2204 Size: 937 997 865 298 2916 Fragments arranged by size: 2916 997 937 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2287 2561 2580 2829 4676 882 Size: 617 788 274 19 249 1847 2219 Fragments arranged by size: 2219 1847 788 617 274 249 19 BsaAI yAC'GTr Cuts at: 493 1204 3804 493 Size: 711 2600 2702 Fragments arranged by size: 2702 2600 711 BsaBI GATnn'nnATC Cuts at: 6007 6007 Size: 6013 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2637 3820 4202 4837 Size: 53 83 186 936 1104 1183 382 635 Cuts at: 4837 275 Size: 1451 Fragments arranged by size: 1451 1183 1104 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2858 4446 4844 5118 5265 5676 2858 Size: 1588 398 274 147 411 3195 Fragments arranged by size: 3195 1588 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2192 2222 1178 Size: 30 165 30 789 30 4969 Fragments arranged by size: 4969 789 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2285 2673 1186 Size: 1099 388 4526 Fragments arranged by size: 4526 1099 388 BseYI C'CCAG_C Cuts at: 2049 2178 5216 5712 2049 Size: 129 3038 496 2350 Fragments arranged by size: 3038 2350 496 129 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5440 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2254 3463 4224 5252 1540 Size: 275 186 253 1209 761 1028 2301 Fragments arranged by size: 2301 1209 1028 761 275 253 186 BsiHKAI G_wGCw'C Cuts at: 2564 4074 4159 5230 2564 Size: 1510 85 1071 3347 Fragments arranged by size: 3347 1510 1071 85 BsmI GAATG_Cn' Cuts at: 2161 3524 3617 2161 Size: 1363 93 4557 Fragments arranged by size: 4557 1363 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2545 2545 Size: 6013 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2564 3332 4074 4159 5230 1391 Size: 399 774 768 742 85 1071 2174 Fragments arranged by size: 2174 1071 774 768 742 399 85 BspEI T'CCGG_A Cuts at: 2858 5676 2858 Size: 2818 3195 BspHI T'CATG_A Cuts at: 3902 5632 3902 Size: 1730 4283 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2305 2847 3049 3368 844 Size: 1461 542 202 319 3489 Fragments arranged by size: 3489 1461 542 319 202 BsrBI CCG'CTC Cuts at: 1394 1823 3900 1394 Size: 429 2077 3507 Fragments arranged by size: 3507 2077 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4508 4682 65 Size: 1183 3260 174 1396 Fragments arranged by size: 3260 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4657 4844 1700 Size: 110 2847 187 2869 Fragments arranged by size: 2869 2847 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6013 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6013 BssSI C'ACGA_G Cuts at: 4073 5085 4073 Size: 1012 5001 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6013 BstBI TT'CG_AA Cuts at: 4817 4817 Size: 6013 BstEII G'GTnAC_C Cuts at: 1184 2401 1184 Size: 1217 4796 BstXI CCAn_nnnn'nTGG Cuts at: 2648 2648 Size: 6013 BstYI r'GATC_y Cuts at: 1972 2283 2749 3343 3710 4095 4112 5564 5650 Size: 311 466 594 367 385 17 1452 86 Cuts at: 5650 5662 6008 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 594 466 385 367 346 311 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4522 Fragments arranged by size: 4522 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5274 Fragments arranged by size: 5274 683 56 BtsI GCAGTG_nn' Cuts at: 3209 3271 3529 4323 4343 3209 Size: 62 258 794 20 4879 Fragments arranged by size: 4879 794 258 62 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6013 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5978 DraI TTT'AAA Cuts at: 3317 3317 Size: 6013 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5020 809 Size: 507 3704 1802 Fragments arranged by size: 3704 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2111 2251 3071 Size: 54 1425 50 275 18 281 140 820 Cuts at: 3071 3460 4349 5978 8 Size: 389 889 1629 43 Fragments arranged by size: 1629 1425 889 820 389 281 275 140 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2251 3460 1537 Size: 275 439 1209 4090 Fragments arranged by size: 4090 1209 439 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2497 2583 3007 3093 3179 3943 1353 Size: 1144 86 424 86 86 764 3423 Fragments arranged by size: 3423 1144 764 424 86 86 86 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4600 4974 5120 387 Size: 1064 138 3011 374 146 1280 Fragments arranged by size: 3011 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2049 2384 2434 2573 2988 4076 4673 Size: 393 417 335 50 139 415 1088 597 Cuts at: 4673 5460 5728 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1088 787 597 417 415 393 335 268 139 50 EcoNI CCTnn'n_nnAGG Cuts at: 2379 2379 Size: 6013 EcoRI G'AATT_C Cuts at: 3322 3700 3322 Size: 378 5635 FspI TGC'GCA Cuts at: 2160 2189 4519 2160 Size: 29 2330 3654 Fragments arranged by size: 3654 2330 29 HaeII r_GCGC'y Cuts at: 1678 5160 1678 Size: 3482 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2286 2318 2558 2560 2590 Size: 32 135 32 714 32 240 2 30 Cuts at: 2590 2592 2716 2748 2908 2940 4654 4686 4728 Size: 2 124 32 160 32 1714 32 42 Cuts at: 4728 4760 1373 Size: 32 2626 Fragments arranged by size: 2626 1714 714 240 160 135 124 42 32 32 32 32 32 32 32 30 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 3357 3603 669 Size: 988 281 1419 246 3079 Fragments arranged by size: 3079 1419 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6013 HpaI GTT'AAC Cuts at: 3603 3603 Size: 6013 KpnI G_GTAC'C Cuts at: 3338 3338 Size: 6013 MfeI C'AATT_G Cuts at: 2642 3612 2642 Size: 970 5043 MluI A'CGCG_T Cuts at: 5889 5889 Size: 6013 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5127 5311 1305 Size: 3822 184 2007 Fragments arranged by size: 3822 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2113 3073 10 Size: 54 1425 343 281 960 2950 Fragments arranged by size: 2950 1425 960 343 281 54 MslI CAynn'nnrTG Cuts at: 518 1166 3971 4330 4489 518 Size: 648 2805 359 159 2042 Fragments arranged by size: 2805 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2164 2178 2332 2970 3083 3429 4106 5254 Size: 558 14 154 638 113 346 677 1148 Cuts at: 5254 5499 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 638 558 346 245 154 113 14 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4522 Fragments arranged by size: 4522 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6013 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6013 NotI GC'GGCC_GC Cuts at: 2251 3460 2251 Size: 1209 4804 NspI r_CATG'y Cuts at: 1132 1229 3113 3371 4916 1132 Size: 97 1884 258 1545 2229 Fragments arranged by size: 2229 1884 1545 258 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6013 PciI A'CATG_T Cuts at: 3109 4912 3109 Size: 1803 4210 PflMI CCAn_nnn'nTGG Cuts at: 1108 2412 1108 Size: 1304 4709 PfoI T'CCnGG_A Cuts at: 2103 2628 2103 Size: 525 5488 PmeI GTTT'AAAC Cuts at: 3317 3317 Size: 6013 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4082 4114 5595 5627 4082 Size: 32 1481 32 4468 Fragments arranged by size: 4468 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6013 PsiI TTA'TAA Cuts at: 3583 3583 Size: 6013 PstI C_TGCA'G Cuts at: 830 3046 3365 830 Size: 2216 319 3478 Fragments arranged by size: 3478 2216 319 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6013 PvuII CAG'CTG Cuts at: 2178 2970 2178 Size: 792 5221 SalI G'TCGA_C Cuts at: 1936 3355 1936 Size: 1419 4594 SapI GCTCTTCn'nnn_ Cuts at: 2497 2497 Size: 6013 SbfI CC_TGCA'GG Cuts at: 3365 3365 Size: 6013 ScaI AGT'ACT Cuts at: 4261 5973 4261 Size: 1712 4301 SexAI A'CCwGG_T Cuts at: 2570 2570 Size: 6013 SfcI C'TryA_G Cuts at: 826 1045 2137 2824 3042 3361 4496 5177 5368 Size: 219 1092 687 218 319 1135 681 191 Cuts at: 5368 826 Size: 1471 Fragments arranged by size: 1471 1135 1092 687 681 319 219 218 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6013 SmaI CCC'GGG Cuts at: 1872 3340 1872 Size: 1468 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2219 3156 4117 5018 5280 820 Size: 197 926 276 937 961 901 262 1553 Fragments arranged by size: 1553 961 937 926 901 276 262 197 SnaBI TAC'GTA Cuts at: 493 3804 493 Size: 3311 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6013 SphI G_CATG'C Cuts at: 1132 3371 1132 Size: 2239 3774 SspI AAT'ATT Cuts at: 5 52 3206 3937 5 Size: 47 3154 731 2081 Fragments arranged by size: 3154 2081 731 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6013 StyI C'CwwG_G Cuts at: 513 1065 2004 3452 513 Size: 552 939 1448 3074 Fragments arranged by size: 3074 1448 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2225 4040 4057 4210 4395 4840 325 Size: 1255 645 1815 17 153 185 445 1498 Fragments arranged by size: 1815 1498 1255 645 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3106 4259 5971 Size: 274 80 33 51 1443 1129 1153 1712 Cuts at: 5971 96 Size: 138 Fragments arranged by size: 1712 1443 1153 1129 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5973 6005 5973 Size: 32 5981 Tth111I GACn'n_nGTC Cuts at: 1316 2572 1316 Size: 1256 4757 XbaI T'CTAG_A Cuts at: 3349 3349 Size: 6013 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6013 XmaI C'CCGG_G Cuts at: 1870 3338 1870 Size: 1468 4545 XmnI GAAnn'nnTTC Cuts at: 4142 4142 Size: 6013 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3821 276 Size: 53 83 186 936 2287 2468 Fragments arranged by size: 2468 2287 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI