Restriction map for
pFN21AE5247
(Circular) (Six-base) MAPSORT of: pFN21AE5247.seq Check: 7617 from: 1 to: 5371
pFN21AE5247 5371 bp
With 182 enzymes: SgfI *
January 30, 2014 17:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3181 278
Size: 53 83 186 936 1645 2468
Fragments arranged by size:
2468 1645 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2714 1937
Size: 777 4594
Acc65I G'GTAC_C
Cuts at: 2692 2692
Size: 5371
AclI AA'CG_TT
Cuts at: 1563 3498 3871 1563
Size: 1935 373 3063
Fragments arranged by size:
3063 1935 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2567 3434 4818 5086 1632
Size: 935 867 1384 268 1917
Fragments arranged by size:
1917 1384 935 867 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5174
AflIII A'CryG_T
Cuts at: 1203 4270 5247 1203
Size: 3067 977 1327
Fragments arranged by size:
3067 1327 977
AgeI A'CCGG_T
Cuts at: 4202 4202
Size: 5371
AhdI GACnn_n'nnGTC
Cuts at: 4100 4100
Size: 5371
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5371
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5371
AlwNI CAG_nnn'CTG
Cuts at: 3114 4686 3114
Size: 1572 3799
ApaLI G'TGCA_C
Cuts at: 3428 4584 3428
Size: 1156 4215
ApoI r'AATT_y
Cuts at: 1493 2102 2680 2906 3058 5167 1493
Size: 609 578 226 152 2109 1697
Fragments arranged by size:
2109 1697 609 578 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3925 160
Size: 872 2893 1606
Fragments arranged by size:
2893 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5371
AvaI C'yCGr_G
Cuts at: 1870 1943 2696 1870
Size: 73 753 4545
Fragments arranged by size:
4545 753 73
BamHI G'GATC_C
Cuts at: 2701 3068 2701
Size: 367 5004
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2692 4147 618
Size: 325 217 627 905 1455 1842
Fragments arranged by size:
1842 1455 905 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2275 2690 1391
Size: 884 415 4072
Fragments arranged by size:
4072 884 415
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4400
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3562 3596 1903
Size: 34 1625 34 3678
Fragments arranged by size:
3678 1625 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3263 4473 3263
Size: 1210 4161
BclI T'GATC_A
Cuts at: 1548 2373 1548
Size: 825 4546
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3982 136
Size: 107 122 71 631 605 2310 1525
Fragments arranged by size:
2310 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5366 5366
Size: 5371
BlpI GC'TnA_GC
Cuts at: 2462 2788 2462
Size: 326 5045
Bme1580I G_kGCm'C
Cuts at: 1790 3432 4588 1790
Size: 1642 1156 2573
Fragments arranged by size:
2573 1642 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5371
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3102 4060 442
Size: 416 53 83 399 24 1685 958 1753
Fragments arranged by size:
1753 1685 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5371
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2234 2533 2234
Size: 299 5072
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2103 2503 2515 4031 1239
Size: 864 400 12 1516 2579
Fragments arranged by size:
2579 1516 864 400 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3496 4361 4659 3496
Size: 865 298 4208
Fragments arranged by size:
4208 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4034 882
Size: 617 2535 2219
Fragments arranged by size:
2535 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3162 493
Size: 711 1958 2702
Fragments arranged by size:
2702 1958 711
BsaBI GATnn'nnATC
Cuts at: 5365 5365
Size: 5371
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3178 3560 4195 275
Size: 53 83 186 936 1645 382 635 1451
Fragments arranged by size:
1645 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3804 4202 4476 4623 5034 3804
Size: 398 274 147 411 4141
Fragments arranged by size:
4141 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5146
Fragments arranged by size:
5146 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2522 1186
Size: 1336 4035
BseYI C'CCAG_C
Cuts at: 4574 5070 4574
Size: 496 4875
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2222 1163
Size: 573 486 4312
Fragments arranged by size:
4312 573 486
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2821 3582 4610 1540
Size: 275 186 820 761 1028 2301
Fragments arranged by size:
2301 1028 820 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3432 3517 4588 3432
Size: 85 1071 4215
Fragments arranged by size:
4215 1071 85
BsmI GAATG_Cn'
Cuts at: 2882 2975 2882
Size: 93 5278
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2275 2690 3432 3517 4588 1391
Size: 399 485 415 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 485 415 399 85
BspEI T'CCGG_A
Cuts at: 5034 5034
Size: 5371
BspHI T'CATG_A
Cuts at: 3260 4990 3260
Size: 1730 3641
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2583 2726 844
Size: 1739 143 3489
Fragments arranged by size:
3489 1739 143
BsrBI CCG'CTC
Cuts at: 1394 1823 3258 1394
Size: 429 1435 3507
Fragments arranged by size:
3507 1435 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2226 3866 4040 65
Size: 1183 978 1640 174 1396
Fragments arranged by size:
1640 1396 1183 978 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4015 4202 1700
Size: 110 2205 187 2869
Fragments arranged by size:
2869 2205 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5371
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5371
BssSI C'ACGA_G
Cuts at: 3431 4443 3431
Size: 1012 4359
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5371
BstBI TT'CG_AA
Cuts at: 4175 4175
Size: 5371
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5371
BstYI r'GATC_y
Cuts at: 1972 2701 3068 3453 3470 4922 5008 5020 5366
Size: 729 367 385 17 1452 86 12 346
Cuts at: 5366 1972
Size: 1977
Fragments arranged by size:
1977 1452 729 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2346 2346
Size: 5371
BtgI C'CryG_G
Cuts at: 513 1065 2004 2431 2625 513
Size: 552 939 427 194 3259
Fragments arranged by size:
3259 939 552 427 194
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4632
Fragments arranged by size:
4632 683 56
BtsI GCAGTG_nn'
Cuts at: 2887 3681 3701 2887
Size: 794 20 4557
Fragments arranged by size:
4557 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5371
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5336
DraI TTT'AAA
Cuts at: 2675 2675
Size: 5371
DraIII CAC_nnn'GTG
Cuts at: 2064 2064
Size: 5371
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4378 809
Size: 507 3062 1802
Fragments arranged by size:
3062 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2818 3707 5336
Size: 54 1425 50 275 18 988 889 1629
Cuts at: 5336 8
Size: 43
Fragments arranged by size:
1629 1425 988 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2818 1537
Size: 275 1006 4090
Fragments arranged by size:
4090 1006 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2091 3301 1353
Size: 738 1210 3423
Fragments arranged by size:
3423 1210 738
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3958 4332 4478 387
Size: 1064 138 2369 374 146 1280
Fragments arranged by size:
2369 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2103 2503 2515 2567 3434 4031 4818
Size: 393 471 400 12 52 867 597 787
Cuts at: 4818 5086 1239
Size: 268 1524
Fragments arranged by size:
1524 867 787 597 471 400 393 268
52 12
EcoO109I rG'GnC_Cy
Cuts at: 2551 2551
Size: 5371
EcoRI G'AATT_C
Cuts at: 2680 3058 2680
Size: 378 4993
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2386 2462 2386
Size: 76 5295
FspI TGC'GCA
Cuts at: 3877 3877
Size: 5371
HaeII r_GCGC'y
Cuts at: 1678 4518 1678
Size: 2840 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2621 2653 4012 4044 4086
Size: 32 135 32 1049 32 1359 32 42
Cuts at: 4086 4118 1373
Size: 32 2626
Fragments arranged by size:
2626 1359 1049 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2715 2961 669
Size: 988 281 777 246 3079
Fragments arranged by size:
3079 988 777 281 246
HindIII A'AGCT_T
Cuts at: 748 2363 748
Size: 1615 3756
HpaI GTT'AAC
Cuts at: 2961 2961
Size: 5371
KpnI G_GTAC'C
Cuts at: 2696 2696
Size: 5371
MfeI C'AATT_G
Cuts at: 2970 2970
Size: 5371
MluI A'CGCG_T
Cuts at: 5247 5247
Size: 5371
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4485 4669 1305
Size: 3180 184 2007
Fragments arranged by size:
3180 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3549
Fragments arranged by size:
3549 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3329 3688 3847 518
Size: 648 2163 359 159 2042
Fragments arranged by size:
2163 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2081 2787 3464 4612 4857 1606
Size: 475 706 677 1148 245 2120
Fragments arranged by size:
2120 1148 706 677 475 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2431 2625 513
Size: 552 939 427 194 3259
Fragments arranged by size:
3259 939 552 427 194
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5371
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5371
NotI GC'GGCC_GC
Cuts at: 2818 2818
Size: 5371
NspI r_CATG'y
Cuts at: 1132 1229 2729 4274 1132
Size: 97 1500 1545 2229
Fragments arranged by size:
2229 1545 1500 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5371
PciI A'CATG_T
Cuts at: 4270 4270
Size: 5371
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2064 1108
Size: 956 4415
PmeI GTTT'AAAC
Cuts at: 2675 2675
Size: 5371
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3440 3472 4953 4985 3440
Size: 32 1481 32 3826
Fragments arranged by size:
3826 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2520 1613
Size: 907 4464
PsiI TTA'TAA
Cuts at: 2108 2941 2108
Size: 833 4538
PstI C_TGCA'G
Cuts at: 830 2080 2119 2723 830
Size: 1250 39 604 3478
Fragments arranged by size:
3478 1250 604 39
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5371
PvuII CAG'CTG
Cuts at: 2081 2081
Size: 5371
SalI G'TCGA_C
Cuts at: 1936 2713 1936
Size: 777 4594
SbfI CC_TGCA'GG
Cuts at: 2723 2723
Size: 5371
ScaI AGT'ACT
Cuts at: 3619 5331 3619
Size: 1712 3659
SfcI C'TryA_G
Cuts at: 826 1045 2076 2115 2597 2719 3854 4535 4726
Size: 219 1031 39 482 122 1135 681 191
Cuts at: 4726 826
Size: 1471
Fragments arranged by size:
1471 1135 1031 681 482 219 191 122
39
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5371
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5371
SmaI CCC'GGG
Cuts at: 1872 2698 1872
Size: 826 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3475 4376 4638 820
Size: 197 926 1532 901 262 1553
Fragments arranged by size:
1553 1532 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3162 493
Size: 2669 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5371
SphI G_CATG'C
Cuts at: 1132 2729 1132
Size: 1597 3774
SspI AAT'ATT
Cuts at: 5 52 3295 5
Size: 47 3243 2081
Fragments arranged by size:
3243 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5371
StyI C'CwwG_G
Cuts at: 513 1065 2004 2431 2612 2625 2810 513
Size: 552 939 427 181 13 185 3074
Fragments arranged by size:
3074 939 552 427 185 181 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2300 3398 3415 3568 3753 4198 325
Size: 1255 720 1098 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1098 720 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3617 5329 96
Size: 274 80 33 51 1443 1640 1712 138
Fragments arranged by size:
1712 1640 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5331 5363 5331
Size: 32 5339
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5371
XbaI T'CTAG_A
Cuts at: 2707 2707
Size: 5371
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5371
XmaI C'CCGG_G
Cuts at: 1870 2696 1870
Size: 826 4545
XmnI GAAnn'nnTTC
Cuts at: 2660 3500 2660
Size: 840 4531
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3179 276
Size: 53 83 186 936 1645 2468
Fragments arranged by size:
2468 1645 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI