(Circular) (Six-base) MAPSORT of: pFN21AE1647.seq Check: 3846 from: 1 to: 5194 pFN21AE1647 5194 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2471 2471 Size: 5194 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3004 278 Size: 53 83 186 936 1468 2468 Fragments arranged by size: 2468 1468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2537 1937 Size: 600 4594 Acc65I G'GTAC_C Cuts at: 2280 2515 2280 Size: 235 4959 AclI AA'CG_TT Cuts at: 1563 3321 3694 1563 Size: 1758 373 3063 Fragments arranged by size: 3063 1758 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3257 4641 4909 1632 Size: 1625 1384 268 1917 Fragments arranged by size: 1917 1625 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4997 AflIII A'CryG_T Cuts at: 1203 4093 5070 1203 Size: 2890 977 1327 Fragments arranged by size: 2890 1327 977 AgeI A'CCGG_T Cuts at: 4025 4025 Size: 5194 AhdI GACnn_n'nnGTC Cuts at: 3923 3923 Size: 5194 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5194 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5194 AlwNI CAG_nnn'CTG Cuts at: 2937 4509 2937 Size: 1572 3622 ApaLI G'TGCA_C Cuts at: 3251 4407 3251 Size: 1156 4038 ApoI r'AATT_y Cuts at: 1493 2359 2503 2729 2881 4990 1493 Size: 866 144 226 152 2109 1697 Fragments arranged by size: 2109 1697 866 226 152 144 AseI AT'TA_AT Cuts at: 160 1032 3748 160 Size: 872 2716 1606 Fragments arranged by size: 2716 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5194 AvaI C'yCGr_G Cuts at: 1870 1943 2519 1870 Size: 73 576 4545 Fragments arranged by size: 4545 576 73 BamHI G'GATC_C Cuts at: 2524 2891 2524 Size: 367 4827 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2280 2515 3970 618 Size: 325 217 627 493 235 1455 1842 Fragments arranged by size: 1842 1455 627 493 325 235 217 BanII G_rGCy'C Cuts at: 1391 2513 1391 Size: 1122 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2021 2352 928 Size: 971 122 331 3770 Fragments arranged by size: 3770 971 331 122 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3385 3419 1903 Size: 34 1448 34 3678 Fragments arranged by size: 3678 1448 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3086 4296 3086 Size: 1210 3984 BclI T'GATC_A Cuts at: 1548 1548 Size: 5194 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3805 136 Size: 107 122 71 631 605 2133 1525 Fragments arranged by size: 2133 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5189 5189 Size: 5194 BlpI GC'TnA_GC Cuts at: 2611 2611 Size: 5194 Bme1580I G_kGCm'C Cuts at: 1790 3255 4411 1790 Size: 1465 1156 2573 Fragments arranged by size: 2573 1465 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5194 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2299 2925 3883 Size: 416 53 83 399 24 882 626 958 Cuts at: 3883 442 Size: 1753 Fragments arranged by size: 1753 958 882 626 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5194 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2251 2251 Size: 5194 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3854 1239 Size: 2615 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2319 3319 4184 4482 2319 Size: 1000 865 298 3031 Fragments arranged by size: 3031 1000 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2375 3857 882 Size: 617 876 1482 2219 Fragments arranged by size: 2219 1482 876 617 BsaAI yAC'GTr Cuts at: 493 1204 2219 2985 493 Size: 711 1015 766 2702 Fragments arranged by size: 2702 1015 766 711 BsaBI GATnn'nnATC Cuts at: 5188 5188 Size: 5194 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3001 3383 4018 275 Size: 53 83 186 936 1468 382 635 1451 Fragments arranged by size: 1468 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2394 3627 4025 4299 4446 4857 2394 Size: 1233 398 274 147 411 2731 Fragments arranged by size: 2731 1233 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4969 Fragments arranged by size: 4969 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5194 BseYI C'CCAG_C Cuts at: 2029 4397 4893 2029 Size: 2368 496 2330 Fragments arranged by size: 2368 2330 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2443 2510 1163 Size: 573 707 67 3847 Fragments arranged by size: 3847 707 573 67 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2644 3405 4433 1540 Size: 275 186 643 761 1028 2301 Fragments arranged by size: 2301 1028 761 643 275 186 BsiHKAI G_wGCw'C Cuts at: 3255 3340 4411 3255 Size: 85 1071 4038 Fragments arranged by size: 4038 1071 85 BsmI GAATG_Cn' Cuts at: 2705 2798 2705 Size: 93 5101 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2513 3255 3340 4411 1391 Size: 399 723 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 723 399 85 BspEI T'CCGG_A Cuts at: 2394 4857 2394 Size: 2463 2731 BspHI T'CATG_A Cuts at: 3083 4813 3083 Size: 1730 3464 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2471 2482 2549 844 Size: 1627 11 67 3489 Fragments arranged by size: 3489 1627 67 11 BsrBI CCG'CTC Cuts at: 1394 1823 2235 3081 1394 Size: 429 412 846 3507 Fragments arranged by size: 3507 846 429 412 BsrDI GCAATG_nn' Cuts at: 65 1248 3689 3863 65 Size: 1183 2441 174 1396 Fragments arranged by size: 2441 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3838 4025 1700 Size: 110 2028 187 2869 Fragments arranged by size: 2869 2028 187 110 BsrGI T'GTAC_A Cuts at: 96 2296 96 Size: 2200 2994 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5194 BssSI C'ACGA_G Cuts at: 3254 4266 3254 Size: 1012 4182 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5194 BstBI TT'CG_AA Cuts at: 3998 3998 Size: 5194 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5194 BstYI r'GATC_y Cuts at: 1972 2524 2891 3276 3293 4745 4831 4843 5189 Size: 552 367 385 17 1452 86 12 346 Cuts at: 5189 1972 Size: 1977 Fragments arranged by size: 1977 1452 552 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2034 513 Size: 552 969 3673 Fragments arranged by size: 3673 969 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4455 Fragments arranged by size: 4455 683 56 BtsI GCAGTG_nn' Cuts at: 2710 3504 3524 2710 Size: 794 20 4380 Fragments arranged by size: 4380 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5194 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5159 DraI TTT'AAA Cuts at: 2498 2498 Size: 5194 DraIII CAC_nnn'GTG Cuts at: 2488 2488 Size: 5194 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4201 809 Size: 507 2885 1802 Fragments arranged by size: 2885 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2641 3530 5159 Size: 54 1425 50 275 18 811 889 1629 Cuts at: 5159 8 Size: 43 Fragments arranged by size: 1629 1425 889 811 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2641 1537 Size: 275 829 4090 Fragments arranged by size: 4090 829 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3124 1353 Size: 1771 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3781 4155 4301 387 Size: 1064 138 2192 374 146 1280 Fragments arranged by size: 2192 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3257 3854 4641 4909 1239 Size: 393 1625 597 787 268 1524 Fragments arranged by size: 1625 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2154 2154 Size: 5194 EcoO109I rG'GnC_Cy Cuts at: 2269 2269 Size: 5194 EcoRI G'AATT_C Cuts at: 2359 2503 2881 2359 Size: 144 378 4672 Fragments arranged by size: 4672 378 144 FspI TGC'GCA Cuts at: 3700 3700 Size: 5194 HaeII r_GCGC'y Cuts at: 1678 4341 1678 Size: 2663 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3835 3867 3909 3941 1373 Size: 32 135 32 2263 32 42 32 2626 Fragments arranged by size: 2626 2263 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2538 2784 669 Size: 988 281 600 246 3079 Fragments arranged by size: 3079 988 600 281 246 HindIII A'AGCT_T Cuts at: 748 2171 748 Size: 1423 3771 HpaI GTT'AAC Cuts at: 2784 2784 Size: 5194 KpnI G_GTAC'C Cuts at: 2284 2519 2284 Size: 235 4959 MfeI C'AATT_G Cuts at: 2793 2793 Size: 5194 MluI A'CGCG_T Cuts at: 5070 5070 Size: 5194 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2412 4308 4492 1305 Size: 1107 1896 184 2007 Fragments arranged by size: 2007 1896 1107 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3372 Fragments arranged by size: 3372 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2354 3152 3511 3670 518 Size: 648 843 345 798 359 159 2042 Fragments arranged by size: 2042 843 798 648 359 345 159 MspA1I CmG'CkG Cuts at: 1606 2610 3287 4435 4680 1606 Size: 1004 677 1148 245 2120 Fragments arranged by size: 2120 1148 1004 677 245 NcoI C'CATG_G Cuts at: 513 1065 2034 513 Size: 552 969 3673 Fragments arranged by size: 3673 969 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5194 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5194 NotI GC'GGCC_GC Cuts at: 2641 2641 Size: 5194 NspI r_CATG'y Cuts at: 1132 1229 2552 4097 1132 Size: 97 1323 1545 2229 Fragments arranged by size: 2229 1545 1323 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5194 PciI A'CATG_T Cuts at: 4093 4093 Size: 5194 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5194 PmeI GTTT'AAAC Cuts at: 2498 2498 Size: 5194 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3263 3295 4776 4808 3263 Size: 32 1481 32 3649 Fragments arranged by size: 3649 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2269 2269 Size: 5194 PshAI GACnn'nnGTC Cuts at: 1613 2387 1613 Size: 774 4420 PsiI TTA'TAA Cuts at: 2764 2764 Size: 5194 PstI C_TGCA'G Cuts at: 830 2546 830 Size: 1716 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5194 SalI G'TCGA_C Cuts at: 1936 2536 1936 Size: 600 4594 SanDI GG'GwC_CC Cuts at: 2269 2269 Size: 5194 SbfI CC_TGCA'GG Cuts at: 2546 2546 Size: 5194 ScaI AGT'ACT Cuts at: 2045 3442 5154 2045 Size: 1397 1712 2085 Fragments arranged by size: 2085 1712 1397 SfcI C'TryA_G Cuts at: 826 1045 2542 3677 4358 4549 826 Size: 219 1497 1135 681 191 1471 Fragments arranged by size: 1497 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5194 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5194 SmaI CCC'GGG Cuts at: 1872 2521 1872 Size: 649 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2334 3298 4199 4461 820 Size: 197 926 391 964 901 262 1553 Fragments arranged by size: 1553 964 926 901 391 262 197 SnaBI TAC'GTA Cuts at: 493 2985 493 Size: 2492 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5194 SphI G_CATG'C Cuts at: 1132 2552 1132 Size: 1420 3774 SspI AAT'ATT Cuts at: 5 52 3118 5 Size: 47 3066 2081 Fragments arranged by size: 3066 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5194 StyI C'CwwG_G Cuts at: 513 1065 2034 2407 2633 513 Size: 552 969 373 226 3074 Fragments arranged by size: 3074 969 552 373 226 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3221 3238 3391 3576 4021 325 Size: 1255 1641 17 153 185 445 1498 Fragments arranged by size: 1641 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2043 2296 3440 Size: 274 80 33 51 1443 66 253 1144 Cuts at: 3440 5152 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1144 274 253 138 80 66 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5154 5186 5154 Size: 32 5162 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5194 XbaI T'CTAG_A Cuts at: 2530 2530 Size: 5194 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5194 XmaI C'CCGG_G Cuts at: 1870 2519 1870 Size: 649 4545 XmnI GAAnn'nnTTC Cuts at: 3323 3323 Size: 5194 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3002 276 Size: 53 83 186 936 1468 2468 Fragments arranged by size: 2468 1468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI