################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BbrPI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoRV EcoT22I FseI FspI HindIII HpaI KpnI MamI MfeI MluI MroI MunI NaeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SalI SbfI SexAI SfcI SfiI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AcsI 1039 GAATTC [GA]^AATT[CT] AlwNI 1316 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 493 GGGCCC GGGCC^C ApoI 1039 GAATTC [GA]^AATT[CT] AvaI 902 CTCGGG C^[CT]CG[GA]G BamHI 1078 GGATCC G^GATCC BbeI 707 GGCGCC GGCGC^C BbsI 836 GAAGAC GAAGAC(2/6) BbvCI 694 GCTGAGG CCTCAGC(-5/-2) BcgI 894 GCACTTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1142 TGATCA T^GATCA BpuAI 836 GAAGAC GAAGAC(2/6) BsiEI 248 CGGCCG CG[GA][CT]^CG BsmAI 1119 GAGAC GTCTC(1/5) BsmI 464 GAATGC GAATGC(1/-1) BsoBI 902 CTCGGG C^[CT]CG[GA]G BspHI 440 TCATGA T^CATGA BspMI 344 ACCTGC ACCTGC(4/8) BsrDI 690 CATTGC GCAATG(2/0) BsrFI 151 ACCGGC [GA]^CCGG[CT] BstAPI 1168 GCAATATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 684 GGTGACC G^GT[ACGT]ACC BstF5I 1113 CATCC GGATG(2/0) BstPI 684 GGTGACC G^GT[ACGT]ACC Cfr10I 151 ACCGGC [GA]^CCGG[CT] DraII 492 AGGGCCC [GA]G^G[ACGT]CC[CT] EagI 248 CGGCCG C^GGCCG EclXI 248 CGGCCG C^GGCCG Eco52I 248 CGGCCG C^GGCCG EcoO109I 492 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoO65I 684 GGTGACC G^GT[ACGT]ACC EcoRI 1039 GAATTC G^AATTC FbaI 1142 TGATCA T^GATCA FokI 1113 CATCC GGATG(9/13) KasI 707 GGCGCC G^GCGCC KspI 515 CCGCGG CCGC^GG MaeII 42 ACGT A^CGT NarI 707 GGCGCC GG^CGCC NcoI 447 CCATGG C^CATGG NdeI 973 CATATG CA^TATG NspI 1278 ACATGC [GA]CATG^[CT] RcaI 440 TCATGA T^CATGA SacII 515 CCGCGG CCGC^GG SapI 761 GAAGAGC GCTCTTC(1/4) ScaI 299 AGTACT AGT^ACT SfoI 707 GGCGCC GGC^GCC SpeI 21 ACTAGT A^CTAGT TaqI 410 TCGA T^CGA TfiI 1264 GATTC G^A[AT]TC TthHB8I 410 TCGA T^CGA XbaI 1101 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccII 356 CGCG CG^CG 516 CGCG AcyI 262 GACGCC G[GA]^CG[CT]C 707 GGCGCC AfaI 195 GTAC GT^AC 300 GTAC BalI 573 TGGCCA TGG^CCA 1269 TGGCCA BcnI 16 CCGGG CC^[GC]GG 246 CCCGG BfaI 22 CTAG C^TAG 1102 CTAG BpmI 1083 CTCCAG CTGGAG(16/14) 88 CTGGAG BsaHI 262 GACGCC G[GA]^CG[CT]C 707 GGCGCC BseRI 123 CTCCTC GAGGAG(10/8) 126 CTCCTC BsgI 346 CTGCAC GTGCAG(16/14) 1175 GTGCAG BsrBI 202 CCGCTC CCGCTC(-3/-3) 1256 GAGCGG BstUI 356 CGCG CG^CG 516 CGCG BstYI 311 AGATCC [GA]^GATC[CT] 1078 GGATCC Cac8I 357 GCGGGC GC[ACGT]^[ACGT]GC 507 GCTGGC EarI 593 GAAGAG CTCTTC(1/4) 761 GAAGAG HaeII 360 GGCGCT [GA]GCGC^[CT] 707 GGCGCC HgaI 262 GACGC GACGC(5/10) 166 GCGTC Hin1I 262 GACGCC G[GA]^CG[CT]C 707 GGCGCC HincII 3 GTCAAC GT[CT]^[GA]AC 613 GTCAAC HindII 3 GTCAAC GT[CT]^[GA]AC 613 GTCAAC Ksp632I 593 GAAGAG CTCTTC(1/4) 761 GAAGAG MaeI 22 CTAG C^TAG 1102 CTAG MflI 311 AGATCC [GA]^GATC[CT] 1078 GGATCC MluNI 573 TGGCCA TGG^CCA 1269 TGGCCA MscI 573 TGGCCA TGG^CCA 1269 TGGCCA MslI 603 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 719 CACCTGAGTG MspA1I 515 CCGCGG C[AC]G^C[GT]G 786 CCGCTG MvnI 356 CGCG CG^CG 516 CGCG NciI 16 CCGGG CC^[GC]GG 246 CCCGG RsaI 195 GTAC GT^AC 300 GTAC SmlI 364 CTCAAG C^T[CT][GA]AG 1053 CTTGAG XcmI 497 CCAGCCTGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 965 CCAGACAACATATGG XhoII 311 AGATCC [GA]^GATC[CT] 1078 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AluI AlwI BanI BanII BbvI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstNI CfoI Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I FauI Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI StyI Tru9I TseI Tsp45I Tsp509I TspRI