(Linear) (Six-base) MAPSORT of: KIBB5806.seq Check: 321 from: 1 to: 1356 KIBB5806.orf With 182 enzymes: SgfI * March 22, 2012 15:32 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1298 1356 Size: 1298 58 AleI CACnn'nnGTG Cuts at: 0 607 723 1356 Size: 607 116 633 Fragments arranged by size: 633 607 116 AlwNI CAG_nnn'CTG Cuts at: 0 1321 1356 Size: 1321 35 ApaI G_GGCC'C Cuts at: 0 497 1356 Size: 497 859 ApoI r'AATT_y Cuts at: 0 1039 1356 Size: 1039 317 AvaI C'yCGr_G Cuts at: 0 902 1356 Size: 902 454 BamHI G'GATC_C Cuts at: 0 1078 1356 Size: 1078 278 BanI G'GyrC_C Cuts at: 0 154 601 707 1356 Size: 154 447 106 649 Fragments arranged by size: 649 447 154 106 BanII G_rGCy'C Cuts at: 0 329 497 541 1356 Size: 329 168 44 815 Fragments arranged by size: 815 329 168 44 BbeI G_GCGC'C Cuts at: 0 711 1356 Size: 711 645 BbsI GAAGACnn'nnnn_ Cuts at: 0 843 1356 Size: 843 513 BbvCI CC'TCA_GC Cuts at: 0 695 1356 Size: 695 661 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 883 917 1356 Size: 883 34 439 Fragments arranged by size: 883 439 34 BclI T'GATC_A Cuts at: 0 1142 1356 Size: 1142 214 Bme1580I G_kGCm'C Cuts at: 0 497 1356 Size: 497 859 BmrI ACTGGGnnnn_n' Cuts at: 0 546 1084 1356 Size: 546 538 272 Fragments arranged by size: 546 538 272 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 1068 1356 Size: 109 959 288 Fragments arranged by size: 959 288 109 Bpu10I CC'TnA_GC Cuts at: 0 695 1356 Size: 695 661 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 349 1074 1356 Size: 349 725 282 Fragments arranged by size: 725 349 282 BsaHI Gr'CG_yC Cuts at: 0 263 708 1356 Size: 263 445 648 Fragments arranged by size: 648 445 263 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 538 568 1356 Size: 538 30 788 Fragments arranged by size: 788 538 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 114 117 1356 Size: 114 3 1239 Fragments arranged by size: 1239 114 3 BseYI C'CCAG_C Cuts at: 0 399 496 1356 Size: 399 97 860 Fragments arranged by size: 860 399 97 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 331 1196 1356 Size: 331 865 160 Fragments arranged by size: 865 331 160 BsiEI CG_ry'CG Cuts at: 0 251 1356 Size: 251 1105 BsmI GAATG_Cn' Cuts at: 0 470 1356 Size: 470 886 Bsp1286I G_dGCh'C Cuts at: 0 329 497 541 1356 Size: 329 168 44 815 Fragments arranged by size: 815 329 168 44 BspHI T'CATG_A Cuts at: 0 440 1356 Size: 440 916 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 353 1356 Size: 353 1003 BsrBI CCG'CTC Cuts at: 0 204 1258 1356 Size: 204 1054 98 Fragments arranged by size: 1054 204 98 BsrDI GCAATG_nn' Cuts at: 0 689 1356 Size: 689 667 BsrFI r'CCGG_y Cuts at: 0 151 1356 Size: 151 1205 BstAPI GCAn_nnn'nTGC Cuts at: 0 1174 1356 Size: 1174 182 BstEII G'GTnAC_C Cuts at: 0 684 1356 Size: 684 672 BstYI r'GATC_y Cuts at: 0 311 1078 1356 Size: 311 767 278 Fragments arranged by size: 767 311 278 BtgI C'CryG_G Cuts at: 0 318 447 515 1356 Size: 318 129 68 841 Fragments arranged by size: 841 318 129 68 EaeI y'GGCC_r Cuts at: 0 248 509 573 645 1269 1356 Size: 248 261 64 72 624 87 Fragments arranged by size: 624 261 248 87 72 64 EagI C'GGCC_G Cuts at: 0 248 1356 Size: 248 1108 EarI CTCTTCn'nnn_ Cuts at: 0 588 756 1356 Size: 588 168 600 Fragments arranged by size: 600 588 168 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1268 1276 1356 Size: 1268 8 80 Fragments arranged by size: 1268 80 8 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 1068 1298 1356 Size: 109 959 230 58 Fragments arranged by size: 959 230 109 58 EcoO109I rG'GnC_Cy Cuts at: 0 493 1356 Size: 493 863 EcoRI G'AATT_C Cuts at: 0 1039 1356 Size: 1039 317 HaeII r_GCGC'y Cuts at: 0 364 711 1356 Size: 364 347 645 Fragments arranged by size: 645 364 347 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 151 183 253 285 1356 Size: 151 32 70 32 1071 Fragments arranged by size: 1071 151 70 32 32 HincII GTy'rAC Cuts at: 0 5 615 1356 Size: 5 610 741 Fragments arranged by size: 741 610 5 KasI G'GCGC_C Cuts at: 0 707 1356 Size: 707 649 MscI TGG'CCA Cuts at: 0 575 1271 1356 Size: 575 696 85 Fragments arranged by size: 696 575 85 MslI CAynn'nnrTG Cuts at: 0 607 723 1356 Size: 607 116 633 Fragments arranged by size: 633 607 116 MspA1I CmG'CkG Cuts at: 0 517 788 1356 Size: 517 271 568 Fragments arranged by size: 568 517 271 NarI GG'CG_CC Cuts at: 0 708 1356 Size: 708 648 NcoI C'CATG_G Cuts at: 0 447 1356 Size: 447 909 NdeI CA'TA_TG Cuts at: 0 974 1356 Size: 974 382 NspI r_CATG'y Cuts at: 0 1282 1356 Size: 1282 74 PfoI T'CCnGG_A Cuts at: 0 15 1356 Size: 15 1341 PspOMI G'GGCC_C Cuts at: 0 493 1356 Size: 493 863 SacII CC_GC'GG Cuts at: 0 518 1356 Size: 518 838 SapI GCTCTTCn'nnn_ Cuts at: 0 756 1356 Size: 756 600 ScaI AGT'ACT Cuts at: 0 301 1356 Size: 301 1055 SfoI GGC'GCC Cuts at: 0 709 1356 Size: 709 647 SmlI C'TyrA_G Cuts at: 0 364 1053 1356 Size: 364 689 303 Fragments arranged by size: 689 364 303 SpeI A'CTAG_T Cuts at: 0 21 1356 Size: 21 1335 StyI C'CwwG_G Cuts at: 0 447 576 872 1272 1356 Size: 447 129 296 400 84 Fragments arranged by size: 447 400 296 129 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 485 1356 Size: 485 871 TatI w'GTAC_w Cuts at: 0 194 299 1356 Size: 194 105 1057 Fragments arranged by size: 1057 194 105 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 199 231 538 570 1356 Size: 199 32 307 32 786 Fragments arranged by size: 786 307 199 32 32 XbaI T'CTAG_A Cuts at: 0 1101 1356 Size: 1101 255 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 504 972 1356 Size: 504 468 384 Fragments arranged by size: 504 468 384 Enzymes that do cut: AcuI AleI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI Bme1580I BmrI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstEII BstYI BtgI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII KasI MscI MslI MspA1I NarI NcoI NdeI NspI PfoI PspOMI SacII SapI ScaI SfoI SmlI SpeI StyI TaqII TatI TstI XbaI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BciVI BfrBI BglI BglII BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI BsaWI BsiHKAI BsiWI BsmBI BspEI BsrGI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI NaeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SbfI SexAI SfcI SfiI SgfI SgrAI SmaI SnaBI SphI SrfI SspI StuI SwaI Tth111I XhoI XmaI XmnI ZraI