Restriction map for
pFN21AE9607
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII Acc65I AccII AciI AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaJI
BsgI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmBI BsoBI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I FauI
FbaI FseI FspI HaeIII HgaI
Hin1I HindIII HpaI HphI KasI
KpnI KspI MaeI MaeII MamI
MfeI MluI MluNI MscI MslI
MspA1I MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI Sau96I SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI TaqI Tsp45I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 416 GTCTAC GT^[AC][GT]AC
AccIII 633 TCCGGA T^CCGGA
AluI 447 AGCT AG^CT
AlwNI 550 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
BbsI 147 GTCTTC GAAGAC(2/6)
BbvI 196 GCAGC GCAGC(8/12)
BglII 141 AGATCT A^GATCT
BmyI 434 GGGCAC G[AGT]GC[ACT]^C
BpuAI 147 GTCTTC GAAGAC(2/6)
BsaWI 633 TCCGGA [AT]^CCGG[AT]
BseAI 633 TCCGGA T^CCGGA
BseRI 580 GAGGAG GAGGAG(10/8)
BsmFI 376 GTCCC GGGAC(10/14)
BsmI 705 GAATGC GAATGC(1/-1)
Bsp1286I 434 GGGCAC G[AGT]GC[ACT]^C
BspEI 633 TCCGGA T^CCGGA
BssKI 215 CCTGG ^CC[ACGT]GG
BssSI 437 CACGAG CACGAG(-5/-1)
BstF5I 795 GGATG GGATG(2/0)
BstNI 215 CCTGG CC^[AT]GG
BstXI 383 CCAAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 141 AGATCT [GA]^GATC[CT]
Cac8I 199 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 688 GCGC GCG^C
DdeI 644 CTCAG C^T[ACGT]AG
DpnII 142 GATC ^GATC
EcoRII 215 CCTGG ^CC[AT]GG
Fnu4HI 196 GCAGC GC^[ACGT]GC
FokI 795 GGATG GGATG(9/13)
HaeII 687 GGCGCT [GA]GCGC^[CT]
HapII 634 CCGG C^CGG
HhaI 688 GCGC GCG^C
HinP1I 688 GCGC G^CGC
HincII 762 GTCAAC GT[CT]^[GA]AC
HindII 762 GTCAAC GT[CT]^[GA]AC
HpaII 634 CCGG C^CGG
ItaI 196 GCAGC GC^[ACGT]GC
MaeIII 290 GTAAC ^GT[ACGT]AC
MboI 142 GATC ^GATC
MflI 141 AGATCT [GA]^GATC[CT]
MroI 633 TCCGGA T^CCGGA
MspI 634 CCGG C^CGG
MvaI 215 CCTGG CC^[AT]GG
NdeII 142 GATC ^GATC
NlaIII 759 CATG CATG^
NspI 758 GCATGT [GA]CATG^[CT]
Sau3AI 142 GATC ^GATC
ScaI 621 AGTACT AGT^ACT
ScrFI 215 CCTGG CC^[ACGT]GG
SfcI 472 CTACAG C^T[GA][CT]AG
SspI 523 AATATT AAT^ATT
TseI 196 GCAGC G^C[AT]GC
XhoII 141 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 12 enzymes) #
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AcsI 545 AAATTC [GA]^AATT[CT]
680 AAATTT
AfaI 236 GTAC GT^AC
622 GTAC
ApoI 545 AAATTC [GA]^AATT[CT]
680 AAATTT
BsaI 242 GAGACC GGTCTC(1/5)
499 GAGACC
BsrI 517 ACTGG ACTGG(1/-1)
792 ACTGG
EcoT22I 598 ATGCAT ATGCA^T
797 ATGCAT
HinfI 265 GAATC G^A[ACGT]TC
552 GATTC
NsiI 598 ATGCAT ATGCA^T
797 ATGCAT
RsaI 236 GTAC GT^AC
622 GTAC
SapI 753 GAAGAGC GCTCTTC(1/4)
709 GCTCTTC
TfiI 265 GAATC G^A[AT]TC
552 GATTC
TspRI 113 CAGTG CA[GC]TG[ACGT][ACGT]^
358 CAGTG
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
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BsmAI EarI Ksp632I MboII MnlI
MseI SfaNI Tru9I Tsp509I