################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AviII AvrII BbeI BbvCI BcgI BciVI BfrI BglII BlnI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BspEI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstZ17I CfoI Cfr10I CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PflFI PinAI PmeI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I SwaI Tth111I XcmI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcsI 654 GAATTT [GA]^AATT[CT] AocI 1636 CCTGAGG CC^T[ACGT]AGG ApoI 654 GAATTT [GA]^AATT[CT] AseI 2067 ATTAAT AT^TAAT AsnI 2067 ATTAAT AT^TAAT AspHI 368 GAGCTC G[AT]GC[AT]^C BamHI 140 GGATCC G^GATCC BanI 1571 GGTGCC G^G[CT][GA]CC BbrPI 1318 CACGTG CAC^GTG BglI 1687 GCCATCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1224 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1224 GCTCAGC GC^T[ACGT]AGC BsaAI 1318 CACGTG [CT]AC^GT[GA] BsiHKAI 368 GAGCTC G[AT]GC[AT]^C BsmBI 1230 CGTCTC CGTCTC(1/5) BsmI 2034 GAATGC GAATGC(1/-1) Bsp1407I 268 TGTACA T^GTACA BspDI 1182 ATCGAT AT^CGAT BspMI 1213 ACCTGC ACCTGC(4/8) BsrGI 268 TGTACA T^GTACA BstBI 1006 TTCGAA TT^CGAA BstXI 1041 CCAGTGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1636 CCTGAGG CC^T[ACGT]AGG CelII 1224 GCTCAGC GC^T[ACGT]AGC ClaI 1182 ATCGAT AT^CGAT DraIII 1272 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 1636 CCTGAGG CC^T[ACGT]AGG EcoT14I 890 CCTTGG C^C[AT][AT]GG FauI 1025 CCCGC CCCGC(4/6) HincII 1744 GTCAAC GT[CT]^[GA]AC HindII 1744 GTCAAC GT[CT]^[GA]AC HindIII 1242 AAGCTT A^AGCTT NspV 1006 TTCGAA TT^CGAA PaeR7I 536 CTCGAG C^TCGAG PmaCI 1318 CACGTG CAC^GTG PmlI 1318 CACGTG CAC^GTG PpuMI 159 AGGACCT [GA]G^G[AT]CC[CT] PshBI 2067 ATTAAT AT^TAAT PstI 183 CTGCAG CTGCA^G SacI 368 GAGCTC GAGCT^C SfuI 1006 TTCGAA TT^CGAA SmaI 1283 CCCGGG CCC^GGG SspBI 268 TGTACA T^GTACA StuI 1640 AGGCCT AGG^CCT StyI 890 CCTTGG C^C[AT][AT]GG XbaI 357 TCTAGA T^CTAGA XhoI 536 CTCGAG C^TCGAG XmaCI 1283 CCCGGG C^CCGGG XmaI 1283 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AciI 1026 CCGC CCGC(-3/-1) 1128 CCGC AfaI 269 GTAC GT^AC 817 GTAC AvaI 536 CTCGAG C^[CT]CG[GA]G 1283 CCCGGG AvaII 160 GGACC G^G[AT]CC 1280 GGTCC BalI 566 TGGCCA TGG^CCA 850 TGGCCA BbsI 932 GTCTTC GAAGAC(2/6) 2001 GTCTTC BclI 1259 TGATCA T^GATCA 1580 TGATCA BfaI 358 CTAG C^TAG 2044 CTAG BpmI 371 CTCCAG CTGGAG(16/14) 95 CTGGAG BpuAI 932 GTCTTC GAAGAC(2/6) 2001 GTCTTC BsaI 376 GAGACC GGTCTC(1/5) 1632 GAGACC BsoBI 536 CTCGAG C^[CT]CG[GA]G 1283 CCCGGG DraII 159 AGGACCT [GA]G^G[ACGT]CC[CT] 1756 AGGCCCT EaeI 566 TGGCCA [CT]^GGCC[GA] 850 TGGCCA EcoO109I 159 AGGACCT [GA]G^G[ACGT]CC[CT] 1756 AGGCCCT EcoT22I 857 ATGCAT ATGCA^T 2036 ATGCAT FbaI 1259 TGATCA T^GATCA 1580 TGATCA HapII 1284 CCGG C^CGG 1693 CCGG HgaI 1229 GCGTC GACGC(5/10) 1314 GCGTC HpaII 1284 CCGG C^CGG 1693 CCGG MaeI 358 CTAG C^TAG 2044 CTAG MaeII 1319 ACGT A^CGT 1894 ACGT MluNI 566 TGGCCA TGG^CCA 850 TGGCCA MscI 566 TGGCCA TGG^CCA 850 TGGCCA MspI 1284 CCGG C^CGG 1693 CCGG NsiI 857 ATGCAT ATGCA^T 2036 ATGCAT PleI 301 GACTC GAGTC(4/5) 930 GAGTC PvuII 257 CAGCTG CAG^CTG 2027 CAGCTG RsaI 269 GTAC GT^AC 817 GTAC SapI 549 GAAGAGC GCTCTTC(1/4) 1473 GCTCTTC SmlI 395 CTCAAG C^T[CT][GA]AG 536 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AflIII AluI AlwI BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BspLU11I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII EcoRV Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI Sau3AI Sau96I ScrFI SfaNI SfcI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII