################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AvaI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BcnI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccII 2362 CGCG CG^CG AflII 2565 CTTAAG C^TTAAG AseI 472 ATTAAT AT^TAAT AsnI 472 ATTAAT AT^TAAT Asp700I 568 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 1079 TGGCCA TGG^CCA BanII 2401 GGGCTC G[GA]GC[CT]^C BbvCI 417 CCTCAGC CCTCAGC(-5/-2) BclI 1811 TGATCA T^GATCA BfrI 2565 CTTAAG C^TTAAG BsaWI 2051 ACCGGA [AT]^CCGG[AT] BseRI 2377 CTCCTC GAGGAG(10/8) BsmI 2482 GCATTC GAATGC(1/-1) BspLU11I 684 ACATGT A^CATGT BspMI 876 GCAGGT ACCTGC(4/8) BssSI 2062 CTCGTG CACGAG(-5/-1) BstBI 608 TTCGAA TT^CGAA BstUI 2362 CGCG CG^CG BstXI 223 CCATTATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1417 AGATCC [GA]^GATC[CT] DraIII 1648 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRV 1515 GATATC GAT^ATC FauI 1182 GCGGG CCCGC(4/6) FbaI 1811 TGATCA T^GATCA HapII 2052 CCGG C^CGG HincII 1452 GTTGAC GT[CT]^[GA]AC HindII 1452 GTTGAC GT[CT]^[GA]AC HpaII 2052 CCGG C^CGG MflI 1417 AGATCC [GA]^GATC[CT] MluNI 1079 TGGCCA TGG^CCA MscI 1079 TGGCCA TGG^CCA MspI 2052 CCGG C^CGG MvnI 2362 CGCG CG^CG NdeI 563 CATATG CA^TATG NspV 608 TTCGAA TT^CGAA PshBI 472 ATTAAT AT^TAAT SexAI 328 ACCTGGT A^CC[AT]GGT SfuI 608 TTCGAA TT^CGAA SspI 1060 AATATT AAT^ATT XhoII 1417 AGATCC [GA]^GATC[CT] XmnI 568 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AciI 2361 CCGC CCGC(-3/-1) 1182 GCGG AfaI 308 GTAC GT^AC 1688 GTAC AflIII 684 ACATGT A^C[GA][CT]GT 1864 ACACGT AspHI 383 GAGCAC G[AT]GC[AT]^C 898 GTGCTC AspI 775 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2330 GACTGAGTC BbsI 316 GAAGAC GAAGAC(2/6) 733 GAAGAC BpuAI 316 GAAGAC GAAGAC(2/6) 733 GAAGAC BsgI 1968 CTGCAC GTGCAG(16/14) 2249 CTGCAC BsiHKAI 383 GAGCAC G[AT]GC[AT]^C 898 GTGCTC BsrDI 2151 CATTGC GCAATG(2/0) 947 GCAATG BstEII 159 GGTGACC G^GT[ACGT]ACC 597 GGTCACC BstPI 159 GGTGACC G^GT[ACGT]ACC 597 GGTCACC EaeI 48 CGGCCA [CT]^GGCC[GA] 1079 TGGCCA EarI 1256 CTCTTC CTCTTC(1/4) 2370 GAAGAG EcoNI 2177 CCTACAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2392 CCTCTGCTAGG EcoO65I 159 GGTGACC G^GT[ACGT]ACC 597 GGTCACC EcoRI 501 GAATTC G^AATTC 572 GAATTC EcoT14I 176 CCAAGG C^C[AT][AT]GG 797 CCAAGG EcoT22I 2480 ATGCAT ATGCA^T 2666 ATGCAT Ksp632I 1256 CTCTTC CTCTTC(1/4) 2370 GAAGAG MaeII 1678 ACGT A^CGT 1866 ACGT NsiI 2480 ATGCAT ATGCA^T 2666 ATGCAT NspI 684 ACATGT [GA]CATG^[CT] 2457 GCATGT PflFI 775 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2330 GACTGAGTC PflMI 2070 CCATCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2442 CCAGAATCTGG PstI 301 CTGCAG CTGCA^G 822 CTGCAG RsaI 308 GTAC GT^AC 1688 GTAC SapI 2370 GAAGAGC GCTCTTC(1/4) 1255 GCTCTTC SmlI 1664 CTCAAG C^T[CT][GA]AG 2565 CTTAAG StyI 176 CCAAGG C^C[AT][AT]GG 797 CCAAGG Tth111I 775 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2330 GACTGAGTC Van91I 2070 CCATCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2442 CCAGAATCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AcsI AluI AlwI AlwNI ApoI AvaII BanI BbvI BfaI BglI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DsaI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI