################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AflII AgeI AocI AscI AseI AsnI Asp718I AviII AvrII BamHI BbeI BbrPI BbsI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsiWI BspDI BspHI BsrBI BssHII Bst1107I BstBI BstXI BstZ17I Bsu36I CelII ClaI DraI DrdI EagI EclXI Eco52I Eco81I EcoRI EcoT22I FauI FbaI FseI FspI HgaI Hin1I KasI KpnI MamI MluI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccII 969 CGCG CG^CG AflIII 405 ACATGT A^C[GA][CT]GT Alw44I 2140 GTGCAC G^TGCAC AlwNI 1024 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 2120 AGCGCT AGC^GCT ApaI 866 GGGCCC GGGCC^C ApaLI 2140 GTGCAC G^TGCAC Asp700I 1055 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1618 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 960 CTCGGG C^[CT]CG[GA]G BbvCI 1794 CCTCAGC CCTCAGC(-5/-2) BcgI 2159 GCATGGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 513 CCGGG CC^[GC]GG BfaI 1261 CTAG C^TAG BseRI 1734 GAGGAG GAGGAG(10/8) BsiEI 1957 CGGTCG CG[GA][CT]^CG BsmBI 307 CGTCTC CGTCTC(1/5) BsoBI 960 CTCGGG C^[CT]CG[GA]G BspLU11I 405 ACATGT A^CATGT BsrDI 1106 GCAATG GCAATG(2/0) BstAPI 1023 GCAGTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 969 CGCG CG^CG CfoI 2121 GCGC GCG^C CpoI 509 CGGACCG CG^G[AT]CCG DraII 1974 AGGCCCC [GA]G^G[ACGT]CC[CT] DraIII 216 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 968 CCGCGG C^C[GA][CT]GG EarI 973 GAAGAG CTCTTC(1/4) Eco47III 2120 AGCGCT AGC^GCT EcoO109I 1974 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoRV 1783 GATATC GAT^ATC HaeII 2120 AGCGCT [GA]GCGC^[CT] HhaI 2121 GCGC GCG^C HinP1I 2121 GCGC G^CGC HincII 1404 GTTAAC GT[CT]^[GA]AC HindII 1404 GTTAAC GT[CT]^[GA]AC HindIII 1357 AAGCTT A^AGCTT HpaI 1404 GTTAAC GTT^AAC Ksp632I 973 GAAGAG CTCTTC(1/4) KspI 968 CCGCGG CCGC^GG MaeI 1261 CTAG C^TAG MvnI 969 CGCG CG^CG NaeI 931 GCCGGC GCC^GGC NciI 513 CCGGG CC^[GC]GG NgoMIV 931 GCCGGC G^CCGGC PflFI 1618 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 402 CCAACATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 59 CAGCTG CAG^CTG RsrII 509 CGGACCG CG^G[AT]CCG SacI 348 GAGCTC GAGCT^C SacII 968 CCGCGG CCGC^GG ScaI 2003 AGTACT AGT^ACT SexAI 217 ACCAGGT A^CC[AT]GGT SphI 474 GCATGC GCATG^C StuI 1842 AGGCCT AGG^CCT Tth111I 1618 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 402 CCAACATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 211 CCAAACACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1055 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccIII 1313 TCCGGA T^CCGGA 1550 TCCGGA BalI 429 TGGCCA TGG^CCA 1996 TGGCCA BanI 844 GGCACC G^G[CT][GA]CC 928 GGTGCC BglI 442 GCCTATAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1835 GCCCCCAAGGC BsaWI 1313 TCCGGA [AT]^CCGG[AT] 1550 TCCGGA BseAI 1313 TCCGGA T^CCGGA 1550 TCCGGA Bsp1407I 93 TGTACA T^GTACA 767 TGTACA BspEI 1313 TCCGGA T^CCGGA 1550 TCCGGA BsrFI 931 GCCGGC [GA]^CCGG[CT] 1497 GCCGGT BsrGI 93 TGTACA T^GTACA 767 TGTACA BssSI 463 CACGAG CACGAG(-5/-1) 493 CACGAG BstEII 397 GGTGACC G^GT[ACGT]ACC 556 GGTTACC BstPI 397 GGTGACC G^GT[ACGT]ACC 556 GGTTACC Cfr10I 931 GCCGGC [GA]^CCGG[CT] 1497 GCCGGT EcoO65I 397 GGTGACC G^GT[ACGT]ACC 556 GGTTACC MaeII 226 ACGT A^CGT 2060 ACGT MfeI 771 CAATTG C^AATTG 1158 CAATTG MluNI 429 TGGCCA TGG^CCA 1996 TGGCCA MroI 1313 TCCGGA T^CCGGA 1550 TCCGGA MscI 429 TGGCCA TGG^CCA 1996 TGGCCA MspA1I 59 CAGCTG C[AC]G^C[GT]G 968 CCGCGG MunI 771 CAATTG C^AATTG 1158 CAATTG PstI 146 CTGCAG CTGCA^G 2079 CTGCAG SfcI 146 CTGCAG C^T[GA][CT]AG 2079 CTGCAG SspBI 93 TGTACA T^GTACA 767 TGTACA Tsp45I 398 GTGAC ^GT[GC]AC 2021 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AciI AcsI AfaI AhdI AluI AlwI ApoI AspEI AspHI AvaII BanII BbvI BmyI BpmI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EaeI Eam1105I EcoNI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII