Restriction map for
pFN21AB5744
(Circular) (Six-base) MAPSORT of: pFN21AB5744.seq Check: 438 from: 1 to: 6943
pFN21AB5744 6943 bp
With 182 enzymes: SgfI *
May 12, 2014 10:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4077 4132 4077
Size: 55 6888
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4753 278
Size: 53 83 186 936 3217 2468
Fragments arranged by size:
3217 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4286 1937
Size: 2349 4594
Acc65I G'GTAC_C
Cuts at: 4264 4264
Size: 6943
AclI AA'CG_TT
Cuts at: 1563 5070 5443 1563
Size: 3507 373 3063
Fragments arranged by size:
3507 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2109 3051 3350 3831 3997 5006 6390 6658
Size: 477 942 299 481 166 1009 1384 268
Cuts at: 6658 1632
Size: 1917
Fragments arranged by size:
1917 1384 1009 942 481 477 299 268
166
AfeI AGC'GCT
Cuts at: 4127 4127
Size: 6943
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6746
AflIII A'CryG_T
Cuts at: 1203 2410 5842 6819 1203
Size: 1207 3432 977 1327
Fragments arranged by size:
3432 1327 1207 977
AgeI A'CCGG_T
Cuts at: 5774 5774
Size: 6943
AhdI GACnn_n'nnGTC
Cuts at: 3060 3080 3643 5672 3060
Size: 20 563 2029 4331
Fragments arranged by size:
4331 2029 563 20
AleI CACnn'nnGTG
Cuts at: 1166 4152 1166
Size: 2986 3957
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6943
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2457 2489 3551 3583 2457
Size: 32 1062 32 5817
Fragments arranged by size:
5817 1062 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3034 4686 6258 3034
Size: 1652 1572 3719
Fragments arranged by size:
3719 1652 1572
ApaI G_GGCC'C
Cuts at: 2875 2875
Size: 6943
ApaLI G'TGCA_C
Cuts at: 4145 5000 6156 4145
Size: 855 1156 4932
Fragments arranged by size:
4932 1156 855
ApoI r'AATT_y
Cuts at: 1493 2550 3258 3322 4252 4478 4630 6739 1493
Size: 1057 708 64 930 226 152 2109 1697
Fragments arranged by size:
2109 1697 1057 930 708 226 152 64
AseI AT'TA_AT
Cuts at: 160 1032 5497 160
Size: 872 4465 1606
Fragments arranged by size:
4465 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6943
AvaI C'yCGr_G
Cuts at: 1870 1943 2965 4268 1870
Size: 73 1022 1303 4545
Fragments arranged by size:
4545 1303 1022 73
BamHI G'GATC_C
Cuts at: 4273 4640 4273
Size: 367 6576
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2849 2933 4264 5719 618
Size: 325 217 627 1062 84 1331 1455 1842
Fragments arranged by size:
1842 1455 1331 1062 627 325 217 84
BanII G_rGCy'C
Cuts at: 1391 2357 2875 3374 3842 4262 1391
Size: 966 518 499 468 420 4072
Fragments arranged by size:
4072 966 518 499 468 420
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5972
BbvCI CC'TCA_GC
Cuts at: 3800 3800
Size: 6943
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4153 4187 5134 5168 1903
Size: 34 2216 34 947 34 3678
Fragments arranged by size:
3678 2216 947 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4835 6045 4835
Size: 1210 5733
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6943
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2453 3846 5554
Size: 107 122 71 631 605 781 1393 1708
Cuts at: 5554 136
Size: 1525
Fragments arranged by size:
1708 1525 1393 781 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6938 6938
Size: 6943
BlpI GC'TnA_GC
Cuts at: 4360 4360
Size: 6943
Bme1580I G_kGCm'C
Cuts at: 1790 2852 2875 3227 4149 5004 6160 1790
Size: 1062 23 352 922 855 1156 2573
Fragments arranged by size:
2573 1156 1062 922 855 352 23
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6943
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2878 3261 4674
Size: 416 53 83 399 24 1461 383 1413
Cuts at: 4674 5632 442
Size: 958 1753
Fragments arranged by size:
1753 1461 1413 958 416 399 383 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6943
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2728 2728
Size: 6943
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2916 3014 3515 3594 3724 4007 5603 1239
Size: 1677 98 501 79 130 283 1596 2579
Fragments arranged by size:
2579 1677 1596 501 283 130 98 79
Bpu10I CC'TnA_GC
Cuts at: 2889 3800 3932 2889
Size: 911 132 5900
Fragments arranged by size:
5900 911 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2300 3359 3469 3556 5068 5933 6231 2300
Size: 1059 110 87 1512 865 298 3012
Fragments arranged by size:
3012 1512 1059 865 298 110 87
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2205 3883 4011 5606 882
Size: 617 706 1678 128 1595 2219
Fragments arranged by size:
2219 1678 1595 706 617 128
BsaAI yAC'GTr
Cuts at: 493 1204 4734 493
Size: 711 3530 2702
Fragments arranged by size:
3530 2702 711
BsaBI GATnn'nnATC
Cuts at: 6937 6937
Size: 6943
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4750 5132 5767 275
Size: 53 83 186 936 3217 382 635 1451
Fragments arranged by size:
3217 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3318 3555 5376 5774 6048 6195 6606 3318
Size: 237 1821 398 274 147 411 3655
Fragments arranged by size:
3655 1821 411 398 274 237 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6718
Fragments arranged by size:
6718 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3754 1186
Size: 2568 4375
BseYI C'CCAG_C
Cuts at: 3947 6146 6642 3947
Size: 2199 496 4248
Fragments arranged by size:
4248 2199 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3155 3490 4160 1163
Size: 573 1419 335 670 3946
Fragments arranged by size:
3946 1419 670 573 335
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3965 4393 5154 6182 1540
Size: 275 186 1964 428 761 1028 2301
Fragments arranged by size:
2301 1964 1028 761 428 275 186
BsiHKAI G_wGCw'C
Cuts at: 2357 2890 3189 4149 5004 5089 6160 2357
Size: 533 299 960 855 85 1071 3140
Fragments arranged by size:
3140 1071 960 855 533 299 85
BsmI GAATG_Cn'
Cuts at: 2028 2278 2674 4454 4547 2028
Size: 250 396 1780 93 4424
Fragments arranged by size:
4424 1780 396 250 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2318 2318
Size: 6943
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2357 2852 2875 2890 3189 3227 3374
Size: 399 567 495 23 15 299 38 147
Cuts at: 3374 3842 4149 4262 5004 5089 6160 1391
Size: 468 307 113 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 567 495 468 399 307
299 147 113 85 38 23 15
BspEI T'CCGG_A
Cuts at: 3318 3555 6606 3318
Size: 237 3051 3655
Fragments arranged by size:
3655 3051 237
BspHI T'CATG_A
Cuts at: 4832 6562 4832
Size: 1730 5213
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3865 4077 4132 4298 844
Size: 3021 212 55 166 3489
Fragments arranged by size:
3489 3021 212 166 55
BsrBI CCG'CTC
Cuts at: 1394 1823 4830 1394
Size: 429 3007 3507
Fragments arranged by size:
3507 3007 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3118 5438 5612 65
Size: 1183 1870 2320 174 1396
Fragments arranged by size:
2320 1870 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2936 3502 5587 5774 1700
Size: 110 1126 566 2085 187 2869
Fragments arranged by size:
2869 2085 1126 566 187 110
BsrGI T'GTAC_A
Cuts at: 96 2098 2772 96
Size: 2002 674 4267
Fragments arranged by size:
4267 2002 674
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6943
BssSI C'ACGA_G
Cuts at: 2468 2498 5003 6015 2468
Size: 30 2505 1012 3396
Fragments arranged by size:
3396 2505 1012 30
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3034 794
Size: 2240 4703
BstBI TT'CG_AA
Cuts at: 5747 5747
Size: 6943
BstEII G'GTnAC_C
Cuts at: 1184 2402 2561 1184
Size: 1218 159 5566
Fragments arranged by size:
5566 1218 159
BstYI r'GATC_y
Cuts at: 1972 2018 2472 2697 4273 4640 5025 5042 6494
Size: 46 454 225 1576 367 385 17 1452
Cuts at: 6494 6580 6592 6938 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1576 1452 454 385 367 346 225
86 46 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2973 513
Size: 552 939 969 4483
Fragments arranged by size:
4483 969 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4198 487
Size: 683 56 2972 3232
Fragments arranged by size:
3232 2972 683 56
BtsI GCAGTG_nn'
Cuts at: 3035 3478 4159 4459 5253 5273 3035
Size: 443 681 300 794 20 4705
Fragments arranged by size:
4705 794 681 443 300 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6943
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6908
DraI TTT'AAA
Cuts at: 4247 4247
Size: 6943
DraIII CAC_nnn'GTG
Cuts at: 2226 2226
Size: 6943
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5950 809
Size: 507 4634 1802
Fragments arranged by size:
4634 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2434 3500 4001
Size: 54 1425 50 275 18 604 1066 501
Cuts at: 4001 4390 5279 6908 8
Size: 389 889 1629 43
Fragments arranged by size:
1629 1425 1066 889 604 501 389 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4390 1537
Size: 275 2578 4090
Fragments arranged by size:
4090 2578 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2973 4873 1353
Size: 1620 1900 3423
Fragments arranged by size:
3423 1900 1620
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5530 5904 6050 387
Size: 1064 138 3941 374 146 1280
Fragments arranged by size:
3941 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2355 2355
Size: 6943
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2109 2916 3014 3051 3350 3515 3594
Size: 393 477 807 98 37 299 165 79
Cuts at: 3594 3724 3831 3997 4007 5006 5603 6390 6658
Size: 130 107 166 10 999 597 787 268
Cuts at: 6658 1239
Size: 1524
Fragments arranged by size:
1524 999 807 787 597 477 393 299
268 166 165 130 107 98 79 37
10
EcoNI CCTnn'n_nnAGG
Cuts at: 3218 3539 3935 3218
Size: 321 396 6226
Fragments arranged by size:
6226 396 321
EcoO109I rG'GnC_Cy
Cuts at: 3980 3980
Size: 6943
EcoRI G'AATT_C
Cuts at: 4252 4630 4252
Size: 378 6565
EcoRV GAT'ATC
Cuts at: 3790 3790
Size: 6943
FspI TGC'GCA
Cuts at: 5449 5449
Size: 6943
HaeII r_GCGC'y
Cuts at: 1678 4129 6090 1678
Size: 2451 1961 2531
Fragments arranged by size:
2531 2451 1961
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2065 2097 2296 2328 2615
Size: 32 135 32 493 32 199 32 287
Cuts at: 2615 2647 3046 3066 3078 3098 3629 3661 5584
Size: 32 399 20 12 20 531 32 1923
Cuts at: 5584 5616 5658 5690 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1923 531 493 399 287 199 135
42 32 32 32 32 32 32 32
32 20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3411 4287 4533 669
Size: 988 281 1473 876 246 3079
Fragments arranged by size:
3079 1473 988 876 281 246
HindIII A'AGCT_T
Cuts at: 748 3362 748
Size: 2614 4329
HpaI GTT'AAC
Cuts at: 3411 4533 3411
Size: 1122 5821
KpnI G_GTAC'C
Cuts at: 4268 4268
Size: 6943
MfeI C'AATT_G
Cuts at: 2776 3163 4542 2776
Size: 387 1379 5177
Fragments arranged by size:
5177 1379 387
MluI A'CGCG_T
Cuts at: 6819 6819
Size: 6943
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6057 6241 1305
Size: 4752 184 2007
Fragments arranged by size:
4752 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2436 4003 10
Size: 54 1425 343 604 1567 2950
Fragments arranged by size:
2950 1567 1425 604 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3773 4058 4152 4901 5260 5419 518
Size: 648 2607 285 94 749 359 159 2042
Fragments arranged by size:
2607 2042 749 648 359 285 159 94
MspA1I CmG'CkG
Cuts at: 1606 2066 2975 4359 5036 6184 6429 1606
Size: 460 909 1384 677 1148 245 2120
Fragments arranged by size:
2120 1384 1148 909 677 460 245
NaeI GCC'GGC
Cuts at: 2938 2938
Size: 6943
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5452
Fragments arranged by size:
5452 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6943
NgoMIV G'CCGG_C
Cuts at: 2936 2936
Size: 6943
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6943
NotI GC'GGCC_GC
Cuts at: 4390 4390
Size: 6943
NspI r_CATG'y
Cuts at: 1132 1229 2414 2483 4106 4301 5846 1132
Size: 97 1185 69 1623 195 1545 2229
Fragments arranged by size:
2229 1623 1545 1185 195 97 69
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6943
PciI A'CATG_T
Cuts at: 2410 5842 2410
Size: 3432 3511
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2413 1108
Size: 902 403 5638
Fragments arranged by size:
5638 902 403
PfoI T'CCnGG_A
Cuts at: 3492 3492
Size: 6943
PmeI GTTT'AAAC
Cuts at: 4247 4247
Size: 6943
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5012 5044 6525 6557 5012
Size: 32 1481 32 5398
Fragments arranged by size:
5398 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6943
PsiI TTA'TAA
Cuts at: 4513 4513
Size: 6943
PspOMI G'GGCC_C
Cuts at: 2871 2871
Size: 6943
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2756 2788 3649 3681 2756
Size: 32 861 32 6018
Fragments arranged by size:
6018 861 32 32
PstI C_TGCA'G
Cuts at: 830 2155 4088 4295 830
Size: 1325 1933 207 3478
Fragments arranged by size:
3478 1933 1325 207
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6943
PvuII CAG'CTG
Cuts at: 2066 2066
Size: 6943
RsrII CG'GwC_CG
Cuts at: 2515 2515
Size: 6943
SacI G_AGCT'C
Cuts at: 2357 2357
Size: 6943
SacII CC_GC'GG
Cuts at: 2976 2976
Size: 6943
SalI G'TCGA_C
Cuts at: 1936 4285 1936
Size: 2349 4594
SbfI CC_TGCA'GG
Cuts at: 4295 4295
Size: 6943
ScaI AGT'ACT
Cuts at: 4010 5191 6903 4010
Size: 1181 1712 4050
Fragments arranged by size:
4050 1712 1181
SexAI A'CCwGG_T
Cuts at: 2222 2222
Size: 6943
SfcI C'TryA_G
Cuts at: 826 1045 2151 4084 4291 5426 6107 6298 826
Size: 219 1106 1933 207 1135 681 191 1471
Fragments arranged by size:
1933 1471 1135 1106 681 219 207 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6943
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6943
SmaI CCC'GGG
Cuts at: 1872 4270 1872
Size: 2398 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2315 3338 3448 3571 5047 5948
Size: 197 926 372 1023 110 123 1476 901
Cuts at: 5948 6210 820
Size: 262 1553
Fragments arranged by size:
1553 1476 1023 926 901 372 262 197
123 110
SnaBI TAC'GTA
Cuts at: 493 4734 493
Size: 4241 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6943
SphI G_CATG'C
Cuts at: 1132 2483 4301 1132
Size: 1351 1818 3774
Fragments arranged by size:
3774 1818 1351
SspI AAT'ATT
Cuts at: 5 52 4867 5
Size: 47 4815 2081
Fragments arranged by size:
4815 2081 47
StuI AGG'CCT
Cuts at: 1517 3849 1517
Size: 2332 4611
StyI C'CwwG_G
Cuts at: 513 1065 2004 2437 3844 3859 4235 4382 513
Size: 552 939 433 1407 15 376 147 3074
Fragments arranged by size:
3074 1407 939 552 433 376 147 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2373 3809 4970 4987 5140 5325 5770
Size: 1255 793 1436 1161 17 153 185 445
Cuts at: 5770 325
Size: 1498
Fragments arranged by size:
1498 1436 1255 1161 793 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2098 2772 2810
Size: 274 80 33 51 1443 121 674 38
Cuts at: 2810 4008 5189 6901 96
Size: 1198 1181 1712 138
Fragments arranged by size:
1712 1443 1198 1181 674 274 138 121
80 51 38 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6903 6935 6903
Size: 32 6911
Tth111I GACn'n_nGTC
Cuts at: 1316 3626 1316
Size: 2310 4633
XbaI T'CTAG_A
Cuts at: 4279 4279
Size: 6943
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2223 2223
Size: 6943
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6943
XmaI C'CCGG_G
Cuts at: 1870 4268 1870
Size: 2398 4545
XmnI GAAnn'nnTTC
Cuts at: 3064 5072 3064
Size: 2008 4935
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4751 276
Size: 53 83 186 936 3217 2468
Fragments arranged by size:
3217 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I
Bsu36I FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PpuMI PspXI SanDI SapI SfiI SfoI
SrfI SwaI