(Linear) (Six-base) MAPSORT of: KIBB5744.seq Check: 657 from: 1 to: 2238 KIBB5744.orf With 182 enzymes: SgfI * May 12, 2014 09:13 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2072 2127 2238 Size: 2072 55 111 Fragments arranged by size: 2072 111 55 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 104 1046 1345 1826 1992 2238 Size: 104 942 299 481 166 246 Fragments arranged by size: 942 481 299 246 166 104 AfeI AGC'GCT Cuts at: 0 2122 2238 Size: 2122 116 AflIII A'CryG_T Cuts at: 0 405 2238 Size: 405 1833 AhdI GACnn_n'nnGTC Cuts at: 0 1055 1075 1638 2238 Size: 1055 20 563 600 Fragments arranged by size: 1055 600 563 20 AleI CACnn'nnGTG Cuts at: 0 2147 2238 Size: 2147 91 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 452 484 1546 1578 2238 Size: 452 32 1062 32 660 Fragments arranged by size: 1062 660 452 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 1029 2238 Size: 1029 1209 ApaI G_GGCC'C Cuts at: 0 870 2238 Size: 870 1368 ApaLI G'TGCA_C Cuts at: 0 2140 2238 Size: 2140 98 ApoI r'AATT_y Cuts at: 0 545 1253 1317 2238 Size: 545 708 64 921 Fragments arranged by size: 921 708 545 64 AvaI C'yCGr_G Cuts at: 0 960 2238 Size: 960 1278 BanI G'GyrC_C Cuts at: 0 844 928 2238 Size: 844 84 1310 Fragments arranged by size: 1310 844 84 BanII G_rGCy'C Cuts at: 0 352 870 1369 1837 2238 Size: 352 518 499 468 401 Fragments arranged by size: 518 499 468 401 352 BbvCI CC'TCA_GC Cuts at: 0 1795 2238 Size: 1795 443 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2148 2182 2238 Size: 2148 34 56 Fragments arranged by size: 2148 56 34 BglI GCCn_nnn'nGGC Cuts at: 0 448 1841 2238 Size: 448 1393 397 Fragments arranged by size: 1393 448 397 Bme1580I G_kGCm'C Cuts at: 0 847 870 1222 2144 2238 Size: 847 23 352 922 94 Fragments arranged by size: 922 847 352 94 23 BmrI ACTGGGnnnn_n' Cuts at: 0 873 1256 2238 Size: 873 383 982 Fragments arranged by size: 982 873 383 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 723 2238 Size: 723 1515 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 911 1009 1510 1589 1719 2002 2238 Size: 911 98 501 79 130 283 236 Fragments arranged by size: 911 501 283 236 130 98 79 Bpu10I CC'TnA_GC Cuts at: 0 884 1795 1927 2238 Size: 884 911 132 311 Fragments arranged by size: 911 884 311 132 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 295 1354 1464 1551 2238 Size: 295 1059 110 87 687 Fragments arranged by size: 1059 687 295 110 87 BsaI GGTCTCn'nnnn_ Cuts at: 0 200 1878 2006 2238 Size: 200 1678 128 232 Fragments arranged by size: 1678 232 200 128 BsaWI w'CCGG_w Cuts at: 0 1313 1550 2238 Size: 1313 237 688 Fragments arranged by size: 1313 688 237 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1749 2238 Size: 1749 489 BseYI C'CCAG_C Cuts at: 0 1942 2238 Size: 1942 296 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1150 1485 2155 2238 Size: 1150 335 670 83 Fragments arranged by size: 1150 670 335 83 BsiEI CG_ry'CG Cuts at: 0 1960 2238 Size: 1960 278 BsiHKAI G_wGCw'C Cuts at: 0 352 885 1184 2144 2238 Size: 352 533 299 960 94 Fragments arranged by size: 960 533 352 299 94 BsmI GAATG_Cn' Cuts at: 0 23 273 669 2238 Size: 23 250 396 1569 Fragments arranged by size: 1569 396 250 23 BsmBI CGTCTCn'nnnn_ Cuts at: 0 313 2238 Size: 313 1925 Bsp1286I G_dGCh'C Cuts at: 0 352 847 870 885 1184 1222 1369 1837 Size: 352 495 23 15 299 38 147 468 Cuts at: 1837 2144 2238 Size: 307 94 Fragments arranged by size: 495 468 352 307 299 147 94 38 23 15 BspEI T'CCGG_A Cuts at: 0 1313 1550 2238 Size: 1313 237 688 Fragments arranged by size: 1313 688 237 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1860 2072 2127 2238 Size: 1860 212 55 111 Fragments arranged by size: 1860 212 111 55 BsrDI GCAATG_nn' Cuts at: 0 1113 2238 Size: 1113 1125 BsrFI r'CCGG_y Cuts at: 0 931 1497 2238 Size: 931 566 741 Fragments arranged by size: 931 741 566 BsrGI T'GTAC_A Cuts at: 0 93 767 2238 Size: 93 674 1471 Fragments arranged by size: 1471 674 93 BssSI C'ACGA_G Cuts at: 0 463 493 2238 Size: 463 30 1745 Fragments arranged by size: 1745 463 30 BstAPI GCAn_nnn'nTGC Cuts at: 0 1029 2238 Size: 1029 1209 BstEII G'GTnAC_C Cuts at: 0 397 556 2238 Size: 397 159 1682 Fragments arranged by size: 1682 397 159 BstYI r'GATC_y Cuts at: 0 13 467 692 2238 Size: 13 454 225 1546 Fragments arranged by size: 1546 454 225 13 BtgI C'CryG_G Cuts at: 0 968 2238 Size: 968 1270 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2193 2238 Size: 2193 45 BtsI GCAGTG_nn' Cuts at: 0 1030 1473 2154 2238 Size: 1030 443 681 84 Fragments arranged by size: 1030 681 443 84 DraIII CAC_nnn'GTG Cuts at: 0 221 2238 Size: 221 2017 EaeI y'GGCC_r Cuts at: 0 429 1495 1996 2238 Size: 429 1066 501 242 Fragments arranged by size: 1066 501 429 242 EarI CTCTTCn'nnn_ Cuts at: 0 968 2238 Size: 968 1270 EcoICRI GAG'CTC Cuts at: 0 350 2238 Size: 350 1888 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 104 911 1009 1046 1345 1510 1589 1719 Size: 104 807 98 37 299 165 79 130 Cuts at: 1719 1826 1992 2002 2238 Size: 107 166 10 236 Fragments arranged by size: 807 299 236 166 165 130 107 104 98 79 37 10 EcoNI CCTnn'n_nnAGG Cuts at: 0 1213 1534 1930 2238 Size: 1213 321 396 308 Fragments arranged by size: 1213 396 321 308 EcoO109I rG'GnC_Cy Cuts at: 0 1975 2238 Size: 1975 263 EcoRV GAT'ATC Cuts at: 0 1785 2238 Size: 1785 453 HaeII r_GCGC'y Cuts at: 0 2124 2238 Size: 2124 114 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 60 92 291 323 610 642 1041 1061 Size: 60 32 199 32 287 32 399 20 Cuts at: 1061 1073 1093 1624 1656 2238 Size: 12 20 531 32 582 Fragments arranged by size: 582 531 399 287 199 60 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 0 1406 2238 Size: 1406 832 HindIII A'AGCT_T Cuts at: 0 1357 2238 Size: 1357 881 HpaI GTT'AAC Cuts at: 0 1406 2238 Size: 1406 832 MfeI C'AATT_G Cuts at: 0 771 1158 2238 Size: 771 387 1080 Fragments arranged by size: 1080 771 387 MscI TGG'CCA Cuts at: 0 431 1998 2238 Size: 431 1567 240 Fragments arranged by size: 1567 431 240 MslI CAynn'nnrTG Cuts at: 0 1768 2053 2147 2238 Size: 1768 285 94 91 Fragments arranged by size: 1768 285 94 91 MspA1I CmG'CkG Cuts at: 0 61 970 2238 Size: 61 909 1268 Fragments arranged by size: 1268 909 61 NaeI GCC'GGC Cuts at: 0 933 2238 Size: 933 1305 NgoMIV G'CCGG_C Cuts at: 0 931 2238 Size: 931 1307 NspI r_CATG'y Cuts at: 0 409 478 2101 2238 Size: 409 69 1623 137 Fragments arranged by size: 1623 409 137 69 PciI A'CATG_T Cuts at: 0 405 2238 Size: 405 1833 PflMI CCAn_nnn'nTGG Cuts at: 0 408 2238 Size: 408 1830 PfoI T'CCnGG_A Cuts at: 0 1487 2238 Size: 1487 751 PspOMI G'GGCC_C Cuts at: 0 866 2238 Size: 866 1372 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 751 783 1644 1676 2238 Size: 751 32 861 32 562 Fragments arranged by size: 861 751 562 32 32 PstI C_TGCA'G Cuts at: 0 150 2083 2238 Size: 150 1933 155 Fragments arranged by size: 1933 155 150 PvuII CAG'CTG Cuts at: 0 61 2238 Size: 61 2177 RsrII CG'GwC_CG Cuts at: 0 510 2238 Size: 510 1728 SacI G_AGCT'C Cuts at: 0 352 2238 Size: 352 1886 SacII CC_GC'GG Cuts at: 0 971 2238 Size: 971 1267 ScaI AGT'ACT Cuts at: 0 2005 2238 Size: 2005 233 SexAI A'CCwGG_T Cuts at: 0 217 2238 Size: 217 2021 SfcI C'TryA_G Cuts at: 0 146 2079 2238 Size: 146 1933 159 Fragments arranged by size: 1933 159 146 SmlI C'TyrA_G Cuts at: 0 310 1333 1443 1566 2238 Size: 310 1023 110 123 672 Fragments arranged by size: 1023 672 310 123 110 SphI G_CATG'C Cuts at: 0 478 2238 Size: 478 1760 StuI AGG'CCT Cuts at: 0 1844 2238 Size: 1844 394 StyI C'CwwG_G Cuts at: 0 432 1839 1854 2230 2238 Size: 432 1407 15 376 8 Fragments arranged by size: 1407 432 376 15 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 368 1804 2238 Size: 368 1436 434 Fragments arranged by size: 1436 434 368 TatI w'GTAC_w Cuts at: 0 93 767 805 2003 2238 Size: 93 674 38 1198 235 Fragments arranged by size: 1198 674 235 93 38 Tth111I GACn'n_nGTC Cuts at: 0 1621 2238 Size: 1621 617 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 218 2238 Size: 218 2020 XmnI GAAnn'nnTTC Cuts at: 0 1059 2238 Size: 1059 1179 Enzymes that do cut: AarI AcuI AfeI AflIII AhdI AleI AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbvCI BcgI BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstYI BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I HincII HindIII HpaI MfeI MscI MslI MspA1I NaeI NgoMIV NspI PciI PflMI PfoI PspOMI PsrI PstI PvuII RsrII SacI SacII ScaI SexAI SfcI SmlI SphI StuI StyI TaqII TatI Tth111I XcmI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AlfI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BsaAI BsaBI BsaHI BsaXI BsiWI BspHI BsrBI BssHII BstBI BstXI BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EciI EcoRI FalI FseI FspI FspAI KasI KpnI MluI MmeI NarI NcoI NdeI NheI NotI NruI NsiI PacI PasI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PvuI SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TstI XbaI XhoI XmaI ZraI