################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AccI AccII AclI AflII AflIII AhdI Alw44I AlwNI Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI BalI BbeI BbrPI BbvCI BciVI BclI BfrI BglI BglII BsaAI BsaBI BseRI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I ClaI CpoI DraI DraIII Eam1105I Eco47III EcoO65I EcoRV EcoT22I FbaI FseI HpaI KasI KspI MamI MfeI MluI MluNI MscI MunI MvnI NarI NdeI NheI NruI NsiI NspV PacI PaeR7I PflMI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AatII 888 GACGTC GACGT^C Acc65I 399 GGTACC G^GTACC AccIII 461 TCCGGA T^CCGGA AcyI 888 GACGTC G[GA]^CG[CT]C AfaI 400 GTAC GT^AC AgeI 1346 ACCGGT A^CCGGT AocI 1172 CCTCAGG CC^T[ACGT]AGG ApaI 1059 GGGCCC GGGCC^C Asp718I 399 GGTACC G^GTACC AspI 800 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 629 CTCGGG C^[CT]CG[GA]G AviII 99 TGCGCA TGC^GCA AvrII 964 CCTAGG C^CTAGG BamHI 1312 GGATCC G^GATCC BcgI 1178 GCATGTCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 964 CCTAGG C^CTAGG BlpI 454 GCTGAGC GC^T[ACGT]AGC BpmI 48 CTCCAG CTGGAG(16/14) Bpu1102I 454 GCTGAGC GC^T[ACGT]AGC BsaHI 888 GACGTC G[GA]^CG[CT]C BseAI 461 TCCGGA T^CCGGA BsgI 773 CTGCAC GTGCAG(16/14) BsoBI 629 CTCGGG C^[CT]CG[GA]G BspEI 461 TCCGGA T^CCGGA BstXI 1425 CCAGAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1172 CCTCAGG CC^T[ACGT]AGG CelII 454 GCTGAGC GC^T[ACGT]AGC DraII 1059 GGGCCCT [GA]G^G[ACGT]CC[CT] DrdI 888 GACGTCCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1172 CCTCAGG CC^T[ACGT]AGG EcoNI 390 CCTCGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1059 GGGCCCT [GA]G^G[ACGT]CC[CT] EcoRI 1479 GAATTC G^AATTC FspI 99 TGCGCA TGC^GCA HgaI 1441 GACGC GACGC(5/10) Hin1I 888 GACGTC G[GA]^CG[CT]C HincII 1438 GTTGAC GT[CT]^[GA]AC HindII 1438 GTTGAC GT[CT]^[GA]AC HindIII 655 AAGCTT A^AGCTT KpnI 399 GGTACC GGTAC^C MroI 461 TCCGGA T^CCGGA NaeI 29 GCCGGC GCC^GGC NgoMIV 29 GCCGGC G^CCGGC NotI 237 GCGGCCGC GC^GGCCGC NspI 1178 GCATGT [GA]CATG^[CT] PflFI 800 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1346 ACCGGT A^CCGGT PvuII 218 CAGCTG CAG^CTG RsaI 400 GTAC GT^AC SacI 457 GAGCTC GAGCT^C TaqI 1387 TCGA T^CGA TfiI 1501 GAATC G^A[AT]TC Tsp45I 1168 GTCAC ^GT[GC]AC Tth111I 800 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1387 TCGA T^CGA XcmI 1248 CCAAAGCCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 1413 AAATTT [GA]^AATT[CT] 1479 GAATTC ApoI 1413 AAATTT [GA]^AATT[CT] 1479 GAATTC BanI 399 GGTACC G^G[CT][GA]CC 1332 GGTGCC BsaI 1343 GAGACC GGTCTC(1/5) 1349 GGTCTC BsaWI 461 TCCGGA [AT]^CCGG[AT] 1346 ACCGGT BsiEI 238 CGGCCG CG[GA][CT]^CG 287 CGGCCG BsmAI 1343 GAGAC GTCTC(1/5) 1350 GTCTC BspMI 1220 ACCTGC ACCTGC(4/8) 1042 GCAGGT BsrFI 29 GCCGGC [GA]^CCGG[CT] 1346 ACCGGT BstYI 15 AGATCC [GA]^GATC[CT] 1312 GGATCC Cfr10I 29 GCCGGC [GA]^CCGG[CT] 1346 ACCGGT EaeI 238 CGGCCG [CT]^GGCC[GA] 287 CGGCCG EagI 238 CGGCCG C^GGCCG 287 CGGCCG EarI 1242 CTCTTC CTCTTC(1/4) 1032 GAAGAG EclXI 238 CGGCCG C^GGCCG 287 CGGCCG Eco52I 238 CGGCCG C^GGCCG 287 CGGCCG HaeII 32 GGCGCT [GA]GCGC^[CT] 347 GGCGCT Ksp632I 1242 CTCTTC CTCTTC(1/4) 1032 GAAGAG MaeII 80 ACGT A^CGT 889 ACGT MflI 15 AGATCC [GA]^GATC[CT] 1312 GGATCC MslI 258 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 954 CACCGATGTG MspA1I 218 CAGCTG C[AC]G^C[GT]G 916 CCGCTG NcoI 260 CCATGG C^CATGG 1328 CCATGG PleI 664 GACTC GAGTC(4/5) 804 GAGTC PstI 58 CTGCAG CTGCA^G 221 CTGCAG SapI 1032 GAAGAGC GCTCTTC(1/4) 1241 GCTCTTC SfcI 58 CTGCAG C^T[GA][CT]AG 221 CTGCAG XhoII 15 AGATCC [GA]^GATC[CT] 1312 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AciI AluI AlwI AspHI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpuAI BsaJI BsiHKAI BsiYI BslI BsmFI BsmI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DsaI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI StyI Tru9I TseI Tsp509I TspRI