(Circular) (Six-base) MAPSORT of: pFN21AB9739.seq Check: 5178 from: 1 to: 6226 pFN21AB9739 6226 bp With 182 enzymes: SgfI * March 7, 2011 13:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3038 3038 Size: 6226 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2897 4036 278 Size: 53 83 186 936 1361 1139 2468 Fragments arranged by size: 2468 1361 1139 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3569 1937 Size: 1632 4594 Acc65I G'GTAC_C Cuts at: 2404 3547 2404 Size: 1143 5083 AclI AA'CG_TT Cuts at: 1563 4353 4726 1563 Size: 2790 373 3063 Fragments arranged by size: 3063 2790 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2026 3039 4289 5673 5941 1632 Size: 394 1013 1250 1384 268 1917 Fragments arranged by size: 1917 1384 1250 1013 394 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6029 AflIII A'CryG_T Cuts at: 1203 5125 6102 1203 Size: 3922 977 1327 Fragments arranged by size: 3922 1327 977 AgeI A'CCGG_T Cuts at: 3351 5057 3351 Size: 1706 4520 AhdI GACnn_n'nnGTC Cuts at: 4955 4955 Size: 6226 AleI CACnn'nnGTG Cuts at: 1166 2267 2963 1166 Size: 1101 696 4429 Fragments arranged by size: 4429 1101 696 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6226 AlwNI CAG_nnn'CTG Cuts at: 3969 5541 3969 Size: 1572 4654 ApaI G_GGCC'C Cuts at: 3068 3068 Size: 6226 ApaLI G'TGCA_C Cuts at: 4283 5439 4283 Size: 1156 5070 ApoI r'AATT_y Cuts at: 1493 3418 3484 3535 3761 3913 6022 1493 Size: 1925 66 51 226 152 2109 1697 Fragments arranged by size: 2109 1925 1697 226 152 66 51 AseI AT'TA_AT Cuts at: 160 1032 4780 160 Size: 872 3748 1606 Fragments arranged by size: 3748 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6226 AvaI C'yCGr_G Cuts at: 1870 1943 2634 3551 1870 Size: 73 691 917 4545 Fragments arranged by size: 4545 917 691 73 AvrII C'CTAG_G Cuts at: 2969 2969 Size: 6226 BamHI G'GATC_C Cuts at: 3317 3556 3923 3317 Size: 239 367 5620 Fragments arranged by size: 5620 367 239 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2404 3337 3547 5002 618 Size: 325 217 627 617 933 210 1455 1842 Fragments arranged by size: 1842 1455 933 627 617 325 217 210 BanII G_rGCy'C Cuts at: 1391 2093 2466 3068 3545 1391 Size: 702 373 602 477 4072 Fragments arranged by size: 4072 702 602 477 373 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2563 2832 2919 928 Size: 971 664 269 87 4235 Fragments arranged by size: 4235 971 664 269 87 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3172 3206 4417 4451 1903 Size: 34 50 34 1151 34 1211 34 3678 Fragments arranged by size: 3678 1211 1151 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4118 5328 4118 Size: 1210 5016 BclI T'GATC_A Cuts at: 1548 1548 Size: 6226 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4837 136 Size: 107 122 71 631 605 3165 1525 Fragments arranged by size: 3165 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6221 6221 Size: 6226 BlpI GC'TnA_GC Cuts at: 2460 3643 2460 Size: 1183 5043 Bme1580I G_kGCm'C Cuts at: 1790 2235 2942 3068 4287 5443 1790 Size: 445 707 126 1219 1156 2573 Fragments arranged by size: 2573 1219 1156 707 445 126 BmgBI CAC'GTC Cuts at: 1318 2086 1318 Size: 768 5458 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2721 2952 3071 Size: 416 53 83 399 24 1304 231 119 Cuts at: 3071 3957 4915 442 Size: 886 958 1753 Fragments arranged by size: 1753 1304 958 886 416 399 231 119 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6226 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2038 4886 1239 Size: 799 2848 2579 Fragments arranged by size: 2848 2579 799 Bpu10I CC'TnA_GC Cuts at: 2882 2882 Size: 6226 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4351 5216 5514 4351 Size: 865 298 5063 Fragments arranged by size: 5063 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3342 3360 4889 882 Size: 617 1843 18 1529 2219 Fragments arranged by size: 2219 1843 1529 617 18 BsaAI yAC'GTr Cuts at: 493 1204 4017 493 Size: 711 2813 2702 Fragments arranged by size: 2813 2702 711 BsaBI GATnn'nnATC Cuts at: 6220 6220 Size: 6226 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2894 4033 4415 5050 Size: 53 83 186 936 1361 1139 382 635 Cuts at: 5050 275 Size: 1451 Fragments arranged by size: 1451 1361 1139 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2466 3351 4659 5057 5331 5478 5889 2466 Size: 885 1308 398 274 147 411 2803 Fragments arranged by size: 2803 1308 885 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2566 2596 1178 Size: 30 165 30 1163 30 4808 Fragments arranged by size: 4808 1163 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6226 BseYI C'CCAG_C Cuts at: 5429 5925 5429 Size: 496 5730 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2763 1163 Size: 573 1027 4626 Fragments arranged by size: 4626 1027 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2246 2295 3676 4437 5465 1540 Size: 275 186 245 49 1381 761 1028 2301 Fragments arranged by size: 2301 1381 1028 761 275 245 186 49 BsiHKAI G_wGCw'C Cuts at: 2030 2333 2466 4287 4372 5443 2030 Size: 303 133 1821 85 1071 2813 Fragments arranged by size: 2813 1821 1071 303 133 85 BsmI GAATG_Cn' Cuts at: 2476 2715 3141 3372 3737 3830 2476 Size: 239 426 231 365 93 4872 Fragments arranged by size: 4872 426 365 239 231 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2093 2235 2333 2466 2942 3068 Size: 399 240 63 142 98 133 476 126 Cuts at: 3068 3545 4287 4372 5443 1391 Size: 477 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 477 476 399 240 142 133 126 98 85 63 BspEI T'CCGG_A Cuts at: 2466 5889 2466 Size: 3423 2803 BspHI T'CATG_A Cuts at: 4115 5845 4115 Size: 1730 4496 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3038 3234 3581 844 Size: 2194 196 347 3489 Fragments arranged by size: 3489 2194 347 196 BsrBI CCG'CTC Cuts at: 1394 1823 4113 1394 Size: 429 2290 3507 Fragments arranged by size: 3507 2290 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4721 4895 65 Size: 1183 3473 174 1396 Fragments arranged by size: 3473 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2034 3351 4870 5057 1700 Size: 110 224 1317 1519 187 2869 Fragments arranged by size: 2869 1519 1317 224 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6226 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6226 BssSI C'ACGA_G Cuts at: 4286 5298 4286 Size: 1012 5214 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6226 BstBI TT'CG_AA Cuts at: 5030 5030 Size: 6226 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6226 BstXI CCAn_nnnn'nTGG Cuts at: 3437 3437 Size: 6226 BstYI r'GATC_y Cuts at: 1972 2020 3317 3556 3923 4308 4325 5777 5863 Size: 48 1297 239 367 385 17 1452 86 Cuts at: 5863 5875 6221 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1297 385 367 346 239 86 48 17 12 Bsu36I CC'TnA_GG Cuts at: 3178 3178 Size: 6226 BtgI C'CryG_G Cuts at: 513 1065 2265 2373 2759 2849 3333 513 Size: 552 1200 108 386 90 484 3406 Fragments arranged by size: 3406 1200 552 484 386 108 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2263 487 Size: 683 56 1037 4450 Fragments arranged by size: 4450 1037 683 56 BtsI GCAGTG_nn' Cuts at: 2235 2991 3742 4536 4556 2235 Size: 756 751 794 20 3905 Fragments arranged by size: 3905 794 756 751 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6226 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6191 DraI TTT'AAA Cuts at: 3530 3530 Size: 6226 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2899 5233 809 Size: 507 1583 2334 1802 Fragments arranged by size: 2334 1802 1583 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2243 2292 3673 Size: 54 1425 50 275 18 413 49 1381 Cuts at: 3673 4562 6191 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1381 889 413 275 54 50 49 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2243 2292 3673 1537 Size: 275 431 49 1381 4090 Fragments arranged by size: 4090 1381 431 275 49 EarI CTCTTCn'nnn_ Cuts at: 1353 3032 3253 4156 1353 Size: 1679 221 903 3423 Fragments arranged by size: 3423 1679 903 221 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3190 3363 4813 5187 5333 387 Size: 1064 138 1601 173 1450 374 146 1280 Fragments arranged by size: 1601 1450 1280 1064 374 173 146 138 EcoICRI GAG'CTC Cuts at: 2464 2464 Size: 6226 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2026 2038 3039 4289 4886 5673 5941 Size: 393 394 12 1001 1250 597 787 268 Cuts at: 5941 1239 Size: 1524 Fragments arranged by size: 1524 1250 1001 787 597 394 393 268 12 EcoNI CCTnn'n_nnAGG Cuts at: 2399 2399 Size: 6226 EcoO109I rG'GnC_Cy Cuts at: 3065 3065 Size: 6226 EcoRI G'AATT_C Cuts at: 3484 3535 3913 3484 Size: 51 378 5797 Fragments arranged by size: 5797 378 51 FspI TGC'GCA Cuts at: 2106 4732 2106 Size: 2626 3600 HaeII r_GCGC'y Cuts at: 1678 2041 2356 5373 1678 Size: 363 315 3017 2531 Fragments arranged by size: 3017 2531 363 315 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2727 2759 4867 4899 4941 Size: 32 135 32 1155 32 2108 32 42 Cuts at: 4941 4973 1373 Size: 32 2626 Fragments arranged by size: 2626 2108 1155 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3445 3570 3816 669 Size: 988 281 1507 125 246 3079 Fragments arranged by size: 3079 1507 988 281 246 125 HindIII A'AGCT_T Cuts at: 748 2660 748 Size: 1912 4314 HpaI GTT'AAC Cuts at: 3816 3816 Size: 6226 KpnI G_GTAC'C Cuts at: 2408 3551 2408 Size: 1143 5083 MfeI C'AATT_G Cuts at: 3825 3825 Size: 6226 MluI A'CGCG_T Cuts at: 6102 6102 Size: 6226 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3150 5340 5524 1305 Size: 1845 2190 184 2007 Fragments arranged by size: 2190 2007 1845 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4404 Fragments arranged by size: 4404 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2267 2963 4184 4543 4702 518 Size: 648 1101 696 1221 359 159 2042 Fragments arranged by size: 2042 1221 1101 696 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2225 2923 3642 4319 5467 5712 1606 Size: 619 698 719 677 1148 245 2120 Fragments arranged by size: 2120 1148 719 698 677 619 245 NaeI GCC'GGC Cuts at: 2036 2036 Size: 6226 NcoI C'CATG_G Cuts at: 513 1065 2265 3333 513 Size: 552 1200 1068 3406 Fragments arranged by size: 3406 1200 1068 552 NdeI CA'TA_TG Cuts at: 387 3523 387 Size: 3136 3090 NgoMIV G'CCGG_C Cuts at: 2034 2034 Size: 6226 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6226 NotI GC'GGCC_GC Cuts at: 2243 3673 2243 Size: 1430 4796 NspI r_CATG'y Cuts at: 1132 1229 3187 3584 5129 1132 Size: 97 1958 397 1545 2229 Fragments arranged by size: 2229 1958 1545 397 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6226 PciI A'CATG_T Cuts at: 5125 5125 Size: 6226 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6226 PmeI GTTT'AAAC Cuts at: 3530 3530 Size: 6226 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4295 4327 5808 5840 4295 Size: 32 1481 32 4681 Fragments arranged by size: 4681 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6226 PsiI TTA'TAA Cuts at: 3796 3796 Size: 6226 PspOMI G'GGCC_C Cuts at: 3064 3064 Size: 6226 PstI C_TGCA'G Cuts at: 830 2067 2230 3578 830 Size: 1237 163 1348 3478 Fragments arranged by size: 3478 1348 1237 163 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6226 PvuII CAG'CTG Cuts at: 2225 2225 Size: 6226 SacI G_AGCT'C Cuts at: 2466 2466 Size: 6226 SalI G'TCGA_C Cuts at: 1936 3568 1936 Size: 1632 4594 SapI GCTCTTCn'nnn_ Cuts at: 3032 3253 3032 Size: 221 6005 SbfI CC_TGCA'GG Cuts at: 3578 3578 Size: 6226 ScaI AGT'ACT Cuts at: 4474 6186 4474 Size: 1712 4514 SfcI C'TryA_G Cuts at: 826 1045 2063 2226 3574 4709 5390 5581 826 Size: 219 1018 163 1348 1135 681 191 1471 Fragments arranged by size: 1471 1348 1135 1018 681 219 191 163 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6226 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6226 SmaI CCC'GGG Cuts at: 1872 3553 1872 Size: 1681 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4330 5231 5493 820 Size: 197 926 2387 901 262 1553 Fragments arranged by size: 2387 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4017 493 Size: 3524 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6226 SphI G_CATG'C Cuts at: 1132 3584 1132 Size: 2452 3774 SspI AAT'ATT Cuts at: 5 52 4150 5 Size: 47 4098 2081 Fragments arranged by size: 4098 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6226 StyI C'CwwG_G Cuts at: 513 1065 2265 2969 3333 3665 513 Size: 552 1200 704 364 332 3074 Fragments arranged by size: 3074 1200 704 552 364 332 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4253 4270 4423 4608 5053 325 Size: 1255 2673 17 153 185 445 1498 Fragments arranged by size: 2673 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4472 6184 96 Size: 274 80 33 51 1443 2495 1712 138 Fragments arranged by size: 2495 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2566 2598 2932 2964 3452 3484 6186 6218 2566 Size: 32 334 32 488 32 2702 32 2574 Fragments arranged by size: 2702 2574 488 334 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2808 1316 Size: 1492 4734 XbaI T'CTAG_A Cuts at: 3562 3562 Size: 6226 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3260 3260 Size: 6226 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6226 XmaI C'CCGG_G Cuts at: 1870 3551 1870 Size: 1681 4545 XmnI GAAnn'nnTTC Cuts at: 4355 4355 Size: 6226 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2895 4034 276 Size: 53 83 186 936 1361 1139 2468 Fragments arranged by size: 2468 1361 1139 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI