Restriction map for
pFN21AE6662
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# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvrII BamHI BanII BbrPI BbsI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BpuAI BsaBI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I Cfr10I ClaI DraI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI KspI MaeI
MaeIII MamI MfeI MflI MluI
MroI MspA1I MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tsp45I
Tsp509I Tth111I TthHB8I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 781 GTCTAC GT^[AC][GT]AC
AlwNI 181 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 464 CCCGAG C^[CT]CG[GA]G
AviII 716 TGCGCA TGC^GCA
BalI 452 TGGCCA TGG^CCA
BanI 469 GGCGCC G^G[CT][GA]CC
BbeI 469 GGCGCC GGCGC^C
BbvCI 57 CCTCAGC CCTCAGC(-5/-2)
BlpI 549 GCTCAGC GC^T[ACGT]AGC
BmyI 789 GGGCAC G[AGT]GC[ACT]^C
Bpu1102I 549 GCTCAGC GC^T[ACGT]AGC
BsaAI 784 TACGTG [CT]AC^GT[GA]
BsaI 409 GAGACC GGTCTC(1/5)
BseRI 268 GAGGAG GAGGAG(10/8)
BsgI 234 CTGCAC GTGCAG(16/14)
BsmBI 89 CGTCTC CGTCTC(1/5)
BsmFI 314 GGGAC GGGAC(10/14)
BsoBI 464 CCCGAG C^[CT]CG[GA]G
Bsp1286I 789 GGGCAC G[AGT]GC[ACT]^C
BsrDI 698 GCAATG GCAATG(2/0)
BsrI 793 ACTGG ACTGG(1/-1)
CelII 549 GCTCAGC GC^T[ACGT]AGC
CpoI 65 CGGACCG CG^G[AT]CCG
DraIII 237 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 186 CCTGAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 716 TGCGCA TGC^GCA
HaeII 469 GGCGCC [GA]GCGC^[CT]
HinfI 643 GACTC G^A[ACGT]TC
KasI 469 GGCGCC G^GCGCC
MluNI 452 TGGCCA TGG^CCA
MscI 452 TGGCCA TGG^CCA
MseI 104 TTAA T^TAA
MslI 681 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 469 GGCGCC GG^CGCC
PleI 643 GACTC GAGTC(4/5)
PstI 346 CTGCAG CTGCA^G
RsrII 65 CGGACCG CG^G[AT]CCG
SfoI 469 GGCGCC GGC^GCC
SmlI 604 CTTGAG C^T[CT][GA]AG
Tru9I 104 TTAA T^TAA
XcmI 263 CCACAGAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BcnI 63 CCCGG CC^[GC]GG
733 CCGGG
BpmI 28 CTGGAG CTGGAG(16/14)
675 CTGGAG
DpnII 124 GATC ^GATC
399 GATC
EaeI 452 TGGCCA [CT]^GGCC[GA]
730 TGGCCG
EcoT14I 150 CCTTGG C^C[AT][AT]GG
647 CCTTGG
FauI 413 CCCGC CCCGC(4/6)
758 CCCGC
MboI 124 GATC ^GATC
399 GATC
NciI 63 CCCGG CC^[GC]GG
733 CCGGG
NdeII 124 GATC ^GATC
399 GATC
PpuMI 314 GGGACCC [GA]G^G[AT]CC[CT]
670 GGGTCCT
Sau3AI 124 GATC ^GATC
399 GATC
SfaNI 146 GCATC GCATC(5/9)
680 GCATC
SfcI 222 CTATAG C^T[GA][CT]AG
346 CTGCAG
StyI 150 CCTTGG C^C[AT][AT]GG
647 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AccII AciI AcyI AluI AvaII
BbvI BsaHI BsaJI BsiYI BslI
BsmAI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DraII
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI Ksp632I
MaeII MboII MnlI MspI MvaI
MvnI MwoI NlaIII NlaIV Sau96I
ScrFI TseI TspRI