################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BclI BfrI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI Bsp1286I Bsp1407I BspDI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI HaeII Hin1I HpaI KasI KpnI KspI MaeII MamI MfeI MluI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SapI SbfI ScaI SfcI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 455 GTCGAC GT^[AC][GT]AC AccIII 167 TCCGGA T^CCGGA AlwNI 375 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG AvaI 257 CTCGGG C^[CT]CG[GA]G AviII 527 TGCGCA TGC^GCA BalI 26 TGGCCA TGG^CCA BcgI 249 GCAGGCATCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 262 GGATAC GTATCC(6/5) BcnI 188 CCGGG CC^[GC]GG BfaI 268 CTAG C^TAG BglI 28 GCCACTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaWI 167 TCCGGA [AT]^CCGG[AT] BseAI 167 TCCGGA T^CCGGA BsoBI 257 CTCGGG C^[CT]CG[GA]G BspEI 167 TCCGGA T^CCGGA BspHI 533 TCATGA T^CATGA BspMI 431 ACCTGC ACCTGC(4/8) BsrI 162 CCAGT ACTGG(1/-1) BstAPI 385 GCAGCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 550 TTCGAA TT^CGAA CfoI 528 GCGC GCG^C DraI 154 TTTAAA TTT^AAA EaeI 26 TGGCCA [CT]^GGCC[GA] EcoT14I 596 CCAAGG C^C[AT][AT]GG FspI 527 TGCGCA TGC^GCA HgaI 435 GCGTC GACGC(5/10) HhaI 528 GCGC GCG^C HinP1I 528 GCGC G^CGC HincII 455 GTCGAC GT[CT]^[GA]AC HindII 455 GTCGAC GT[CT]^[GA]AC HindIII 302 AAGCTT A^AGCTT MaeI 268 CTAG C^TAG MluNI 26 TGGCCA TGG^CCA MroI 167 TCCGGA T^CCGGA MscI 26 TGGCCA TGG^CCA MseI 155 TTAA T^TAA MslI 444 CATTCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 188 CCGGG CC^[GC]GG NspI 279 GCATGC [GA]CATG^[CT] NspV 550 TTCGAA TT^CGAA PleI 458 GACTC GAGTC(4/5) RcaI 533 TCATGA T^CATGA SalI 455 GTCGAC G^TCGAC SexAI 358 ACCTGGT A^CC[AT]GGT SfuI 550 TTCGAA TT^CGAA SphI 279 GCATGC GCATG^C StuI 599 AGGCCT AGG^CCT StyI 596 CCAAGG C^C[AT][AT]GG TfiI 592 GAATC G^A[AT]TC Tru9I 155 TTAA T^TAA TspRI 30 CACTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 157 AAATTC [GA]^AATT[CT] 567 GAATTT AfaI 180 GTAC GT^AC 348 GTAC AlwI 483 GATCC GGATC(4/5) 57 GGATC ApoI 157 AAATTC [GA]^AATT[CT] 567 GAATTT BpmI 372 CTCCAG CTGGAG(16/14) 61 CTGGAG BseRI 472 GAGGAG GAGGAG(10/8) 646 GAGGAG BsgI 247 GTGCAG GTGCAG(16/14) 383 GTGCAG BstYI 57 GGATCT [GA]^GATC[CT] 482 AGATCC Cfr13I 3 GGCCC G^G[ACGT]CC 240 GGGCC DpnII 58 GATC ^GATC 483 GATC HinfI 458 GACTC G^A[ACGT]TC 592 GAATC HphI 589 GGTGA GGTGA(8/7) 356 TCACC MaeIII 124 GTCAC ^GT[ACGT]AC 355 GTCAC MboI 58 GATC ^GATC 483 GATC MflI 57 GGATCT [GA]^GATC[CT] 482 AGATCC NdeII 58 GATC ^GATC 483 GATC NlaIV 239 GGGGCC GG[ACGT]^[ACGT]CC 474 GGAGCC RsaI 180 GTAC GT^AC 348 GTAC Sau3AI 58 GATC ^GATC 483 GATC Sau96I 3 GGCCC G^G[ACGT]CC 240 GGGCC SfaNI 253 GCATC GCATC(5/9) 530 GCATC Tsp45I 124 GTCAC ^GT[GC]AC 355 GTCAC Tsp509I 158 AATT ^AATT 568 AATT XhoII 57 GGATCT [GA]^GATC[CT] 482 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AciI AluI BbvI BsaJI BsiYI BslI BssKI BstF5I BstNI Cac8I DdeI EarI EcoRII Fnu4HI FokI HaeIII HapII HpaII ItaI Ksp632I MboII MnlI MspI MvaI MwoI NlaIII ScrFI TaqI TseI TthHB8I