(Circular) (Six-base) MAPSORT of: pFN21AE5056.seq Check: 8375 from: 1 to: 5377 pFN21AE5056 5377 bp With 182 enzymes: SgfI * December 8, 2014 13:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3187 278 Size: 53 83 186 936 1651 2468 Fragments arranged by size: 2468 1651 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2461 2720 1937 Size: 524 259 4594 Fragments arranged by size: 4594 524 259 Acc65I G'GTAC_C Cuts at: 2698 2698 Size: 5377 AclI AA'CG_TT Cuts at: 1563 3504 3877 1563 Size: 1941 373 3063 Fragments arranged by size: 3063 1941 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2522 2531 2693 3440 4824 5092 1632 Size: 890 9 162 747 1384 268 1917 Fragments arranged by size: 1917 1384 890 747 268 162 9 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5180 AflIII A'CryG_T Cuts at: 1203 4276 5253 1203 Size: 3073 977 1327 Fragments arranged by size: 3073 1327 977 AgeI A'CCGG_T Cuts at: 4208 4208 Size: 5377 AhdI GACnn_n'nnGTC Cuts at: 4106 4106 Size: 5377 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5377 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2307 1184 Size: 1123 4254 AlwNI CAG_nnn'CTG Cuts at: 2385 3120 4692 2385 Size: 735 1572 3070 Fragments arranged by size: 3070 1572 735 ApaLI G'TGCA_C Cuts at: 3434 4590 3434 Size: 1156 4221 ApoI r'AATT_y Cuts at: 1493 2162 2572 2686 2912 3064 5173 1493 Size: 669 410 114 226 152 2109 1697 Fragments arranged by size: 2109 1697 669 410 226 152 114 AseI AT'TA_AT Cuts at: 160 1032 3931 160 Size: 872 2899 1606 Fragments arranged by size: 2899 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5377 AvaI C'yCGr_G Cuts at: 1870 1943 2262 2702 1870 Size: 73 319 440 4545 Fragments arranged by size: 4545 440 319 73 BamHI G'GATC_C Cuts at: 2707 3074 2707 Size: 367 5010 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2698 4153 618 Size: 325 217 627 911 1455 1842 Fragments arranged by size: 1842 1455 911 627 325 217 BanII G_rGCy'C Cuts at: 1391 2696 1391 Size: 1305 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4406 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2243 2277 3568 3602 1903 Size: 34 306 34 1291 34 3678 Fragments arranged by size: 3678 1291 306 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2261 3269 4479 2261 Size: 1008 1210 3159 Fragments arranged by size: 3159 1210 1008 BclI T'GATC_A Cuts at: 1548 1548 Size: 5377 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2039 3988 136 Size: 107 122 71 631 605 367 1949 1525 Fragments arranged by size: 1949 1525 631 605 367 122 107 71 BglII A'GATC_T Cuts at: 5372 5372 Size: 5377 BlpI GC'TnA_GC Cuts at: 2794 2794 Size: 5377 Bme1580I G_kGCm'C Cuts at: 1790 3438 4594 1790 Size: 1648 1156 2573 Fragments arranged by size: 2573 1648 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5377 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3108 4066 442 Size: 416 53 83 399 24 1691 958 1753 Fragments arranged by size: 1753 1691 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5377 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2062 2062 Size: 5377 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2087 2362 4037 1239 Size: 848 275 1675 2579 Fragments arranged by size: 2579 1675 848 275 Bpu10I CC'TnA_GC Cuts at: 2420 2420 Size: 5377 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3502 4367 4665 3502 Size: 865 298 4214 Fragments arranged by size: 4214 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4040 882 Size: 617 2541 2219 Fragments arranged by size: 2541 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3168 493 Size: 711 1964 2702 Fragments arranged by size: 2702 1964 711 BsaBI GATnn'nnATC Cuts at: 5371 5371 Size: 5377 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3184 3566 4201 275 Size: 53 83 186 936 1651 382 635 1451 Fragments arranged by size: 1651 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2172 3810 4208 4482 4629 5040 2172 Size: 1638 398 274 147 411 2509 Fragments arranged by size: 2509 1638 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5152 Fragments arranged by size: 5152 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2492 2666 1186 Size: 1306 174 3897 Fragments arranged by size: 3897 1306 174 BseYI C'CCAG_C Cuts at: 2341 4580 5076 2341 Size: 2239 496 2642 Fragments arranged by size: 2642 2239 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2273 2409 1163 Size: 573 537 136 4131 Fragments arranged by size: 4131 573 537 136 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2827 3588 4616 1540 Size: 275 186 826 761 1028 2301 Fragments arranged by size: 2301 1028 826 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3438 3523 4594 3438 Size: 85 1071 4221 Fragments arranged by size: 4221 1071 85 BsmI GAATG_Cn' Cuts at: 2888 2981 2888 Size: 93 5284 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2696 3438 3523 4594 1391 Size: 399 906 742 85 1071 2174 Fragments arranged by size: 2174 1071 906 742 399 85 BspEI T'CCGG_A Cuts at: 2172 5040 2172 Size: 2868 2509 BspHI T'CATG_A Cuts at: 2538 3266 4996 2538 Size: 728 1730 2919 Fragments arranged by size: 2919 1730 728 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2445 2732 844 Size: 1601 287 3489 Fragments arranged by size: 3489 1601 287 BsrBI CCG'CTC Cuts at: 1394 1823 3264 1394 Size: 429 1441 3507 Fragments arranged by size: 3507 1441 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3872 4046 65 Size: 1183 2624 174 1396 Fragments arranged by size: 2624 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4021 4208 1700 Size: 110 2211 187 2869 Fragments arranged by size: 2869 2211 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5377 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5377 BssSI C'ACGA_G Cuts at: 3437 4449 3437 Size: 1012 4365 BstAPI GCAn_nnn'nTGC Cuts at: 794 2396 794 Size: 1602 3775 BstBI TT'CG_AA Cuts at: 2556 4181 2556 Size: 1625 3752 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5377 BstYI r'GATC_y Cuts at: 1972 2062 2487 2707 3074 3459 3476 4928 5014 Size: 90 425 220 367 385 17 1452 86 Cuts at: 5014 5026 5372 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 425 385 367 346 220 90 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3886 Fragments arranged by size: 3886 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4638 Fragments arranged by size: 4638 683 56 BtsI GCAGTG_nn' Cuts at: 2893 3687 3707 2893 Size: 794 20 4563 Fragments arranged by size: 4563 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5377 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5342 DraI TTT'AAA Cuts at: 2161 2681 2161 Size: 520 4857 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4384 809 Size: 507 3068 1802 Fragments arranged by size: 3068 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2824 3713 Size: 54 1425 50 275 18 201 793 889 Cuts at: 3713 5342 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 793 275 201 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2824 1537 Size: 275 1012 4090 Fragments arranged by size: 4090 1012 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2013 2471 2583 3307 1353 Size: 660 458 112 724 3423 Fragments arranged by size: 3423 724 660 458 112 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3964 4338 4484 387 Size: 1064 138 2375 374 146 1280 Fragments arranged by size: 2375 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2362 2522 2531 2693 3440 4037 Size: 393 455 275 160 9 162 747 597 Cuts at: 4037 4824 5092 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 747 597 455 393 275 268 162 160 9 EcoRI G'AATT_C Cuts at: 2686 3064 2686 Size: 378 4999 FspI TGC'GCA Cuts at: 2534 3883 2534 Size: 1349 4028 HaeII r_GCGC'y Cuts at: 1678 4524 1678 Size: 2846 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2582 2614 4018 4050 4092 Size: 32 135 32 1010 32 1404 32 42 Cuts at: 4092 4124 1373 Size: 32 2626 Fragments arranged by size: 2626 1404 1010 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2462 2721 2967 669 Size: 988 281 524 259 246 3079 Fragments arranged by size: 3079 988 524 281 259 246 HindIII A'AGCT_T Cuts at: 748 2307 748 Size: 1559 3818 HpaI GTT'AAC Cuts at: 2967 2967 Size: 5377 KpnI G_GTAC'C Cuts at: 2702 2702 Size: 5377 MfeI C'AATT_G Cuts at: 2976 2976 Size: 5377 MluI A'CGCG_T Cuts at: 5253 5253 Size: 5377 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4491 4675 1305 Size: 3186 184 2007 Fragments arranged by size: 3186 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2033 10 Size: 54 1425 343 201 3354 Fragments arranged by size: 3354 1425 343 201 54 MslI CAynn'nnrTG Cuts at: 518 1166 2453 3335 3694 3853 518 Size: 648 1287 882 359 159 2042 Fragments arranged by size: 2042 1287 882 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2793 3470 4618 4863 1606 Size: 1187 677 1148 245 2120 Fragments arranged by size: 2120 1187 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3886 Fragments arranged by size: 3886 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5377 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5377 NotI GC'GGCC_GC Cuts at: 2824 2824 Size: 5377 NspI r_CATG'y Cuts at: 1132 1229 2288 2735 4280 1132 Size: 97 1059 447 1545 2229 Fragments arranged by size: 2229 1545 1059 447 97 PasI CC'CwG_GG Cuts at: 1348 2616 1348 Size: 1268 4109 PciI A'CATG_T Cuts at: 4276 4276 Size: 5377 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5377 PfoI T'CCnGG_A Cuts at: 2192 2192 Size: 5377 PmeI GTTT'AAAC Cuts at: 2681 2681 Size: 5377 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3446 3478 4959 4991 3446 Size: 32 1481 32 3832 Fragments arranged by size: 3832 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5377 PsiI TTA'TAA Cuts at: 2947 2947 Size: 5377 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2627 2659 2627 Size: 32 5345 PstI C_TGCA'G Cuts at: 830 2729 830 Size: 1899 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5377 SalI G'TCGA_C Cuts at: 1936 2460 2719 1936 Size: 524 259 4594 Fragments arranged by size: 4594 524 259 SbfI CC_TGCA'GG Cuts at: 2729 2729 Size: 5377 ScaI AGT'ACT Cuts at: 3625 5337 3625 Size: 1712 3665 SexAI A'CCwGG_T Cuts at: 2363 2363 Size: 5377 SfcI C'TryA_G Cuts at: 826 1045 2725 3860 4541 4732 826 Size: 219 1680 1135 681 191 1471 Fragments arranged by size: 1680 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5377 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5377 SmaI CCC'GGG Cuts at: 1872 2704 1872 Size: 832 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3481 4382 4644 820 Size: 197 926 1538 901 262 1553 Fragments arranged by size: 1553 1538 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3168 493 Size: 2675 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5377 SphI G_CATG'C Cuts at: 1132 2288 2735 1132 Size: 1156 447 3774 Fragments arranged by size: 3774 1156 447 SspI AAT'ATT Cuts at: 5 52 3301 5 Size: 47 3249 2081 Fragments arranged by size: 3249 2081 47 StuI AGG'CCT Cuts at: 1517 2606 1517 Size: 1089 4288 StyI C'CwwG_G Cuts at: 513 1065 2004 2601 2816 513 Size: 552 939 597 215 3074 Fragments arranged by size: 3074 939 597 552 215 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3404 3421 3574 3759 4204 325 Size: 1255 1824 17 153 185 445 1498 Fragments arranged by size: 1824 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2184 3623 5335 Size: 274 80 33 51 1443 207 1439 1712 Cuts at: 5335 96 Size: 138 Fragments arranged by size: 1712 1443 1439 274 207 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5337 5369 5337 Size: 32 5345 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5377 XbaI T'CTAG_A Cuts at: 2713 2713 Size: 5377 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5377 XmaI C'CCGG_G Cuts at: 1870 2702 1870 Size: 832 4545 XmnI GAAnn'nnTTC Cuts at: 3506 3506 Size: 5377 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3185 276 Size: 53 83 186 936 1651 2468 Fragments arranged by size: 2468 1651 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI