################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FbaI FseI FspI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MslI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tth111I TthHB8I XbaI XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcsI 892 AAATTT [GA]^AATT[CT] AfaI 496 GTAC GT^AC ApoI 892 AAATTT [GA]^AATT[CT] Asp700I 803 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 774 GAGCTC G[AT]GC[AT]^C AvaI 880 CCCGGG C^[CT]CG[GA]G BalI 660 TGGCCA TGG^CCA BamHI 757 GGATCC G^GATCC BfaI 103 CTAG C^TAG BglI 445 GCCCATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsiHKAI 774 GAGCTC G[AT]GC[AT]^C BsoBI 880 CCCGGG C^[CT]CG[GA]G BstAPI 201 GCAGTTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraII 883 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoNI 793 CCTCTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 883 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoT22I 805 ATGCAT ATGCA^T FauI 399 CCCGC CCCGC(4/6) HaeII 485 GGCGCT [GA]GCGC^[CT] HgaI 537 GACGC GACGC(5/10) HphI 231 GGTGA GGTGA(8/7) MaeI 103 CTAG C^TAG MaeIII 316 GTCAC ^GT[ACGT]AC MluNI 660 TGGCCA TGG^CCA MscI 660 TGGCCA TGG^CCA NsiI 805 ATGCAT ATGCA^T PflMI 269 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 537 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 18 CTGCAG CTGCA^G RsaI 496 GTAC GT^AC SacI 774 GAGCTC GAGCT^C SfaNI 395 GCATC GCATC(5/9) SfcI 18 CTGCAG C^T[GA][CT]AG SmaI 880 CCCGGG CCC^GGG SmlI 704 CTCAAG C^T[CT][GA]AG Tsp45I 316 GTCAC ^GT[GC]AC Tsp509I 893 AATT ^AATT Van91I 269 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 717 CCAAACACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 880 CCCGGG C^CCGGG XmaI 880 CCCGGG C^CCGGG XmnI 803 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BanI 82 GGTGCC G^G[CT][GA]CC 342 GGCACC BsgI 956 CTGCAC GTGCAG(16/14) 208 GTGCAG BsmI 803 GAATGC GAATGC(1/-1) 807 GCATTC BstYI 749 AGATCC [GA]^GATC[CT] 757 GGATCC CfoI 156 GCGC GCG^C 486 GCGC HapII 36 CCGG C^CGG 881 CCGG HhaI 156 GCGC GCG^C 486 GCGC HinP1I 156 GCGC G^CGC 486 GCGC HpaII 36 CCGG C^CGG 881 CCGG MaeII 407 ACGT A^CGT 785 ACGT MflI 749 AGATCC [GA]^GATC[CT] 757 GGATCC MseI 404 TTAA T^TAA 671 TTAA MspA1I 21 CAGCTG C[AC]G^C[GT]G 97 CAGCTG MspI 36 CCGG C^CGG 881 CCGG PvuII 21 CAGCTG CAG^CTG 97 CAGCTG Tru9I 404 TTAA T^TAA 671 TTAA TspRI 6 CAGTG CA[GC]TG[ACGT][ACGT]^ 187 CAGTG XhoII 749 AGATCC [GA]^GATC[CT] 757 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AluI AlwI AlwNI AvaII BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I Fnu4HI FokI HaeIII ItaI MboI MboII MnlI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI StyI TseI