(Circular) (Six-base) MAPSORT of: pFN21AE5446.seq Check: 9927 from: 1 to: 5671 pFN21AE5446 5671 bp With 182 enzymes: SgfI * January 30, 2014 17:20 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2189 2189 Size: 5671 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3481 278 Size: 53 83 186 936 1945 2468 Fragments arranged by size: 2468 1945 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3014 1937 Size: 1077 4594 Acc65I G'GTAC_C Cuts at: 2992 2992 Size: 5671 AclI AA'CG_TT Cuts at: 1563 3798 4171 1563 Size: 2235 373 3063 Fragments arranged by size: 3063 2235 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2560 2584 3734 5118 5386 1632 Size: 928 24 1150 1384 268 1917 Fragments arranged by size: 1917 1384 1150 928 268 24 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5474 AflIII A'CryG_T Cuts at: 1203 4570 5547 1203 Size: 3367 977 1327 Fragments arranged by size: 3367 1327 977 AgeI A'CCGG_T Cuts at: 4502 4502 Size: 5671 AhdI GACnn_n'nnGTC Cuts at: 4400 4400 Size: 5671 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5671 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2459 1184 Size: 1275 4396 AlwNI CAG_nnn'CTG Cuts at: 2031 2280 2503 3414 4986 2031 Size: 249 223 911 1572 2716 Fragments arranged by size: 2716 1572 911 249 223 ApaLI G'TGCA_C Cuts at: 3728 4884 3728 Size: 1156 4515 ApoI r'AATT_y Cuts at: 1493 2897 2980 3206 3358 5467 1493 Size: 1404 83 226 152 2109 1697 Fragments arranged by size: 2109 1697 1404 226 152 83 AseI AT'TA_AT Cuts at: 160 1032 4225 160 Size: 872 3193 1606 Fragments arranged by size: 3193 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5671 AvaI C'yCGr_G Cuts at: 1870 1943 2885 2996 1870 Size: 73 942 111 4545 Fragments arranged by size: 4545 942 111 73 BamHI G'GATC_C Cuts at: 2762 3001 3368 2762 Size: 239 367 5065 Fragments arranged by size: 5065 367 239 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2087 2347 2992 4447 618 Size: 325 217 627 300 260 645 1455 1842 Fragments arranged by size: 1842 1455 645 627 325 300 260 217 BanII G_rGCy'C Cuts at: 1391 2022 2700 2783 2941 2990 1391 Size: 631 678 83 158 49 4072 Fragments arranged by size: 4072 678 631 158 83 49 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2365 2374 2566 928 Size: 971 466 9 192 4033 Fragments arranged by size: 4033 971 466 192 9 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3862 3896 1903 Size: 34 50 34 1841 34 3678 Fragments arranged by size: 3678 1841 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3563 4773 3563 Size: 1210 4461 BclI T'GATC_A Cuts at: 1548 1548 Size: 5671 BfrBI ATG'CAT Cuts at: 2812 2812 Size: 5671 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2456 4282 136 Size: 107 122 71 631 605 784 1826 1525 Fragments arranged by size: 1826 1525 784 631 605 122 107 71 BglII A'GATC_T Cuts at: 5666 5666 Size: 5671 BlpI GC'TnA_GC Cuts at: 3088 3088 Size: 5671 Bme1580I G_kGCm'C Cuts at: 1790 2438 2452 3732 4888 1790 Size: 648 14 1280 1156 2573 Fragments arranged by size: 2573 1280 1156 648 14 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5671 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2004 2290 3402 Size: 416 53 83 399 24 587 286 1112 Cuts at: 3402 4360 442 Size: 958 1753 Fragments arranged by size: 1753 1112 958 587 416 399 286 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5671 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2189 2614 2802 4331 1239 Size: 950 425 188 1529 2579 Fragments arranged by size: 2579 1529 950 425 188 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2694 3796 4661 4959 2694 Size: 1102 865 298 3406 Fragments arranged by size: 3406 1102 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4334 882 Size: 617 2835 2219 Fragments arranged by size: 2835 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3462 493 Size: 711 2258 2702 Fragments arranged by size: 2702 2258 711 BsaBI GATnn'nnATC Cuts at: 5665 5665 Size: 5671 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3478 3860 4495 275 Size: 53 83 186 936 1945 382 635 1451 Fragments arranged by size: 1945 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4104 4502 4776 4923 5334 4104 Size: 398 274 147 411 4441 Fragments arranged by size: 4441 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2114 2144 1178 Size: 30 165 30 711 30 4705 Fragments arranged by size: 4705 711 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5671 BseYI C'CCAG_C Cuts at: 2015 2031 2273 2351 2950 4874 5370 2015 Size: 16 242 78 599 1924 496 2316 Fragments arranged by size: 2316 1924 599 496 242 78 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2234 2946 1163 Size: 573 498 712 3888 Fragments arranged by size: 3888 712 573 498 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3121 3882 4910 1540 Size: 275 186 1120 761 1028 2301 Fragments arranged by size: 2301 1120 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2783 3732 3817 4888 2783 Size: 949 85 1071 3566 Fragments arranged by size: 3566 1071 949 85 BsmI GAATG_Cn' Cuts at: 2812 2814 3182 3275 2812 Size: 2 368 93 5208 Fragments arranged by size: 5208 368 93 2 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2438 2452 2700 2783 2941 2990 Size: 399 232 416 14 248 83 158 49 Cuts at: 2990 3732 3817 4888 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 416 399 248 232 158 85 83 49 14 BspEI T'CCGG_A Cuts at: 5334 5334 Size: 5671 BspHI T'CATG_A Cuts at: 3560 5290 3560 Size: 1730 3941 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2085 2189 2206 2354 3026 844 Size: 1241 104 17 148 672 3489 Fragments arranged by size: 3489 1241 672 148 104 17 BsrBI CCG'CTC Cuts at: 1394 1823 3558 1394 Size: 429 1735 3507 Fragments arranged by size: 3507 1735 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4166 4340 65 Size: 1183 2918 174 1396 Fragments arranged by size: 2918 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4315 4502 1700 Size: 110 2505 187 2869 Fragments arranged by size: 2869 2505 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5671 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5671 BssSI C'ACGA_G Cuts at: 3731 4743 3731 Size: 1012 4659 BstAPI GCAn_nnn'nTGC Cuts at: 794 2212 794 Size: 1418 4253 BstBI TT'CG_AA Cuts at: 4475 4475 Size: 5671 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5671 BstYI r'GATC_y Cuts at: 1972 2754 2762 3001 3368 3753 3770 5222 5308 Size: 782 8 239 367 385 17 1452 86 Cuts at: 5308 5320 5666 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 782 385 367 346 239 86 17 12 8 BtgI C'CryG_G Cuts at: 513 1065 2199 2568 2731 513 Size: 552 1134 369 163 3453 Fragments arranged by size: 3453 1134 552 369 163 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4932 Fragments arranged by size: 4932 683 56 BtsI GCAGTG_nn' Cuts at: 3187 3981 4001 3187 Size: 794 20 4857 Fragments arranged by size: 4857 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5671 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5636 DraI TTT'AAA Cuts at: 2975 2975 Size: 5671 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4678 809 Size: 507 3362 1802 Fragments arranged by size: 3362 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2038 2457 2665 Size: 54 1425 50 275 18 208 419 208 Cuts at: 2665 3118 4007 5636 8 Size: 453 889 1629 43 Fragments arranged by size: 1629 1425 889 453 419 275 208 208 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3118 1537 Size: 275 1306 4090 Fragments arranged by size: 4090 1306 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3601 1353 Size: 2248 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2135 4258 4632 4778 387 Size: 1064 138 546 2123 374 146 1280 Fragments arranged by size: 2123 1280 1064 546 374 146 138 EcoICRI GAG'CTC Cuts at: 2781 2781 Size: 5671 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2189 2560 2584 2614 2802 3734 4331 Size: 393 557 371 24 30 188 932 597 Cuts at: 4331 5118 5386 1239 Size: 787 268 1524 Fragments arranged by size: 1524 932 787 597 557 393 371 268 188 30 24 EcoNI CCTnn'n_nnAGG Cuts at: 2802 2802 Size: 5671 EcoO109I rG'GnC_Cy Cuts at: 2889 2889 Size: 5671 EcoRI G'AATT_C Cuts at: 2980 3358 2980 Size: 378 5293 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2179 2179 Size: 5671 FspI TGC'GCA Cuts at: 4177 4177 Size: 5671 HaeII r_GCGC'y Cuts at: 1678 2494 4818 1678 Size: 816 2324 2531 Fragments arranged by size: 2531 2324 816 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2114 2146 4312 4344 4386 Size: 32 135 32 542 32 2166 32 42 Cuts at: 4386 4418 1373 Size: 32 2626 Fragments arranged by size: 2626 2166 542 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3015 3261 669 Size: 988 281 1077 246 3079 Fragments arranged by size: 3079 1077 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5671 HpaI GTT'AAC Cuts at: 3261 3261 Size: 5671 KpnI G_GTAC'C Cuts at: 2996 2996 Size: 5671 MfeI C'AATT_G Cuts at: 3270 3270 Size: 5671 MluI A'CGCG_T Cuts at: 5547 5547 Size: 5671 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2130 2949 4785 4969 1305 Size: 825 819 1836 184 2007 Fragments arranged by size: 2007 1836 825 819 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2667 10 Size: 54 1425 343 835 3014 Fragments arranged by size: 3014 1425 835 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3629 3988 4147 518 Size: 648 2463 359 159 2042 Fragments arranged by size: 2463 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2028 2104 3087 3764 4912 5157 1606 Size: 422 76 983 677 1148 245 2120 Fragments arranged by size: 2120 1148 983 677 422 245 76 NcoI C'CATG_G Cuts at: 513 1065 2199 2568 2731 513 Size: 552 1134 369 163 3453 Fragments arranged by size: 3453 1134 552 369 163 NdeI CA'TA_TG Cuts at: 387 387 Size: 5671 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5671 NotI GC'GGCC_GC Cuts at: 3118 3118 Size: 5671 NsiI A_TGCA'T Cuts at: 2814 2814 Size: 5671 NspI r_CATG'y Cuts at: 1132 1229 3029 4574 1132 Size: 97 1800 1545 2229 Fragments arranged by size: 2229 1800 1545 97 PasI CC'CwG_GG Cuts at: 1348 2536 1348 Size: 1188 4483 PciI A'CATG_T Cuts at: 4570 4570 Size: 5671 PflMI CCAn_nnn'nTGG Cuts at: 1108 2280 1108 Size: 1172 4499 PfoI T'CCnGG_A Cuts at: 2166 2166 Size: 5671 PmeI GTTT'AAAC Cuts at: 2975 2975 Size: 5671 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3740 3772 5253 5285 3740 Size: 32 1481 32 4126 Fragments arranged by size: 4126 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2546 1613 Size: 933 4738 PsiI TTA'TAA Cuts at: 3241 3241 Size: 5671 PstI C_TGCA'G Cuts at: 830 2027 3023 830 Size: 1197 996 3478 Fragments arranged by size: 3478 1197 996 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5671 PvuII CAG'CTG Cuts at: 2028 2104 2028 Size: 76 5595 SacI G_AGCT'C Cuts at: 2783 2783 Size: 5671 SalI G'TCGA_C Cuts at: 1936 3013 1936 Size: 1077 4594 SbfI CC_TGCA'GG Cuts at: 3023 3023 Size: 5671 ScaI AGT'ACT Cuts at: 3919 5631 3919 Size: 1712 3959 SfcI C'TryA_G Cuts at: 826 1045 2023 3019 4154 4835 5026 826 Size: 219 978 996 1135 681 191 1471 Fragments arranged by size: 1471 1135 996 978 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5671 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5671 SmaI CCC'GGG Cuts at: 1872 2887 2998 1872 Size: 1015 111 4545 Fragments arranged by size: 4545 1015 111 SmlI C'TyrA_G Cuts at: 820 1017 1943 2709 3775 4676 4938 820 Size: 197 926 766 1066 901 262 1553 Fragments arranged by size: 1553 1066 926 901 766 262 197 SnaBI TAC'GTA Cuts at: 493 3462 493 Size: 2969 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5671 SphI G_CATG'C Cuts at: 1132 3029 1132 Size: 1897 3774 SspI AAT'ATT Cuts at: 5 52 3595 5 Size: 47 3543 2081 Fragments arranged by size: 3543 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5671 StyI C'CwwG_G Cuts at: 513 1065 2199 2568 2731 3110 513 Size: 552 1134 369 163 379 3074 Fragments arranged by size: 3074 1134 552 379 369 163 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2224 2365 2581 3698 3715 3868 4053 Size: 1255 644 141 216 1117 17 153 185 Cuts at: 4053 4498 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1117 644 445 216 185 153 141 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3917 5629 96 Size: 274 80 33 51 1443 1940 1712 138 Fragments arranged by size: 1940 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5631 5663 5631 Size: 32 5639 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5671 XbaI T'CTAG_A Cuts at: 3007 3007 Size: 5671 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2729 2729 Size: 5671 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5671 XmaI C'CCGG_G Cuts at: 1870 2885 2996 1870 Size: 1015 111 4545 Fragments arranged by size: 4545 1015 111 XmnI GAAnn'nnTTC Cuts at: 2812 3800 2812 Size: 988 4683 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3479 276 Size: 53 83 186 936 1945 2468 Fragments arranged by size: 2468 1945 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI