(Linear) (Six-base) MAPSORT of: KIEE5446.seq Check: 6667 from: 1 to: 966 KIEE5446.orf With 182 enzymes: SgfI * January 29, 2014 12:01 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 184 966 Size: 184 782 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 555 579 966 Size: 555 24 387 Fragments arranged by size: 555 387 24 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 454 966 Size: 454 512 AlwNI CAG_nnn'CTG Cuts at: 0 26 275 498 966 Size: 26 249 223 468 Fragments arranged by size: 468 249 223 26 ApoI r'AATT_y Cuts at: 0 892 966 Size: 892 74 AvaI C'yCGr_G Cuts at: 0 880 966 Size: 880 86 BamHI G'GATC_C Cuts at: 0 757 966 Size: 757 209 BanI G'GyrC_C Cuts at: 0 82 342 966 Size: 82 260 624 Fragments arranged by size: 624 260 82 BanII G_rGCy'C Cuts at: 0 17 695 778 936 966 Size: 17 678 83 158 30 Fragments arranged by size: 678 158 83 30 17 BbsI GAAGACnn'nnnn_ Cuts at: 0 360 369 561 966 Size: 360 9 192 405 Fragments arranged by size: 405 360 192 9 BfrBI ATG'CAT Cuts at: 0 807 966 Size: 807 159 BglI GCCn_nnn'nGGC Cuts at: 0 451 966 Size: 451 515 Bme1580I G_kGCm'C Cuts at: 0 433 447 966 Size: 433 14 519 Fragments arranged by size: 519 433 14 BmrI ACTGGGnnnn_n' Cuts at: 0 285 966 Size: 285 681 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 184 609 797 966 Size: 184 425 188 169 Fragments arranged by size: 425 188 184 169 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 689 966 Size: 689 277 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 109 139 966 Size: 109 30 827 Fragments arranged by size: 827 109 30 BseYI C'CCAG_C Cuts at: 0 10 26 268 346 945 966 Size: 10 16 242 78 599 21 Fragments arranged by size: 599 242 78 21 16 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 229 941 966 Size: 229 712 25 Fragments arranged by size: 712 229 25 BsiHKAI G_wGCw'C Cuts at: 0 778 966 Size: 778 188 BsmI GAATG_Cn' Cuts at: 0 807 809 966 Size: 807 2 157 Fragments arranged by size: 807 157 2 Bsp1286I G_dGCh'C Cuts at: 0 17 433 447 695 778 936 966 Size: 17 416 14 248 83 158 30 Fragments arranged by size: 416 248 158 83 30 17 14 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 80 184 201 349 966 Size: 80 104 17 148 617 Fragments arranged by size: 617 148 104 80 17 BstAPI GCAn_nnn'nTGC Cuts at: 0 207 966 Size: 207 759 BstYI r'GATC_y Cuts at: 0 749 757 966 Size: 749 8 209 Fragments arranged by size: 749 209 8 BtgI C'CryG_G Cuts at: 0 194 563 726 966 Size: 194 369 163 240 Fragments arranged by size: 369 240 194 163 EaeI y'GGCC_r Cuts at: 0 33 452 660 966 Size: 33 419 208 306 Fragments arranged by size: 419 306 208 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 130 966 Size: 130 836 EcoICRI GAG'CTC Cuts at: 0 776 966 Size: 776 190 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 184 555 579 609 797 966 Size: 184 371 24 30 188 169 Fragments arranged by size: 371 188 184 169 30 24 EcoNI CCTnn'n_nnAGG Cuts at: 0 797 966 Size: 797 169 EcoO109I rG'GnC_Cy Cuts at: 0 884 966 Size: 884 82 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 174 966 Size: 174 792 HaeII r_GCGC'y Cuts at: 0 489 966 Size: 489 477 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 109 141 966 Size: 109 32 825 Fragments arranged by size: 825 109 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 125 944 966 Size: 125 819 22 Fragments arranged by size: 819 125 22 MscI TGG'CCA Cuts at: 0 662 966 Size: 662 304 MspA1I CmG'CkG Cuts at: 0 23 99 966 Size: 23 76 867 Fragments arranged by size: 867 76 23 NcoI C'CATG_G Cuts at: 0 194 563 726 966 Size: 194 369 163 240 Fragments arranged by size: 369 240 194 163 NsiI A_TGCA'T Cuts at: 0 809 966 Size: 809 157 PasI CC'CwG_GG Cuts at: 0 531 966 Size: 531 435 PflMI CCAn_nnn'nTGG Cuts at: 0 275 966 Size: 275 691 PfoI T'CCnGG_A Cuts at: 0 161 966 Size: 161 805 PshAI GACnn'nnGTC Cuts at: 0 541 966 Size: 541 425 PstI C_TGCA'G Cuts at: 0 22 966 Size: 22 944 PvuII CAG'CTG Cuts at: 0 23 99 966 Size: 23 76 867 Fragments arranged by size: 867 76 23 SacI G_AGCT'C Cuts at: 0 778 966 Size: 778 188 SfcI C'TryA_G Cuts at: 0 18 966 Size: 18 948 SmaI CCC'GGG Cuts at: 0 882 966 Size: 882 84 SmlI C'TyrA_G Cuts at: 0 704 966 Size: 704 262 StyI C'CwwG_G Cuts at: 0 194 563 726 966 Size: 194 369 163 240 Fragments arranged by size: 369 240 194 163 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 219 360 576 966 Size: 219 141 216 390 Fragments arranged by size: 390 219 216 141 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 724 966 Size: 724 242 XmaI C'CCGG_G Cuts at: 0 880 966 Size: 880 86 XmnI GAAnn'nnTTC Cuts at: 0 807 966 Size: 807 159 Enzymes that do cut: AarI AcuI AlfI AlwNI ApoI AvaI BamHI BanI BanII BbsI BfrBI BglI Bme1580I BmrI BpmI BpuEI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BstAPI BstYI BtgI EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I MmeI MscI MspA1I NcoI NsiI PasI PflMI PfoI PshAI PstI PvuII SacI SfcI SmaI SmlI StyI TaqII XcmI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BclI BglII BlpI BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EarI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MslI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI PacI PciI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XhoI ZraI