################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflII AgeI AhdI Alw44I AlwNI ApaLI AscI Asp718I AspEI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BfrI BglI BlnI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco52I EcoNI EcoO65I EcoRV EcoT22I FseI FspI HgaI HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AatII 358 GACGTC GACGT^C AcyI 358 GACGTC G[GA]^CG[CT]C AocI 432 CCTGAGG CC^T[ACGT]AGG Aor51HI 37 AGCGCT AGC^GCT ApaI 521 GGGCCC GGGCC^C AseI 931 ATTAAT AT^TAAT AsnI 931 ATTAAT AT^TAAT Asp700I 874 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 74 GAGCAC G[AT]GC[AT]^C AspI 937 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 338 GGTCC G^G[AT]CC BalI 622 TGGCCA TGG^CCA BanII 521 GGGCCC G[GA]GC[CT]^C BbsI 961 GTCTTC GAAGAC(2/6) BciVI 786 GGATAC GTATCC(6/5) BclI 1346 TGATCA T^GATCA BlpI 40 GCTAAGC GC^T[ACGT]AGC Bpu1102I 40 GCTAAGC GC^T[ACGT]AGC BpuAI 961 GTCTTC GAAGAC(2/6) BsaAI 1327 TACGTG [CT]AC^GT[GA] BsaHI 358 GACGTC G[GA]^CG[CT]C BsaI 1390 GGTCTC GGTCTC(1/5) BsiHKAI 74 GAGCAC G[AT]GC[AT]^C BsmI 56 GCATTC GAATGC(1/-1) Bsp1407I 858 TGTACA T^GTACA BspHI 177 TCATGA T^CATGA BspLU11I 1481 ACATGT A^CATGT BsrGI 858 TGTACA T^GTACA Bsu36I 432 CCTGAGG CC^T[ACGT]AGG CelII 40 GCTAAGC GC^T[ACGT]AGC CfoI 38 GCGC GCG^C DrdI 1008 GACCAACTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 97 CCACGG C^C[GA][CT]GG Eco47III 37 AGCGCT AGC^GCT Eco81I 432 CCTGAGG CC^T[ACGT]AGG EcoRI 878 GAATTC G^AATTC EcoT14I 817 CCAAGG C^C[AT][AT]GG FbaI 1346 TGATCA T^GATCA HaeII 37 AGCGCT [GA]GCGC^[CT] HhaI 38 GCGC GCG^C Hin1I 358 GACGTC G[GA]^CG[CT]C HinP1I 38 GCGC G^CGC MluNI 622 TGGCCA TGG^CCA MscI 622 TGGCCA TGG^CCA PflFI 937 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 662 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 931 ATTAAT AT^TAAT PvuII 403 CAGCTG CAG^CTG RcaI 177 TCATGA T^CATGA SapI 1141 GAAGAGC GCTCTTC(1/4) SexAI 938 ACCTGGT A^CC[AT]GGT SspBI 858 TGTACA T^GTACA SspI 731 AATATT AAT^ATT StuI 164 AGGCCT AGG^CCT StyI 817 CCAAGG C^C[AT][AT]GG Tth111I 937 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 300 TCTAGA T^CTAGA XcmI 903 CCACCCTGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 874 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AflIII 1328 ACGTGT A^C[GA][CT]GT 1481 ACATGT AlwI 497 GGATC GGATC(4/5) 504 GGATC BglII 866 AGATCT A^GATCT 1364 AGATCT BmyI 74 GAGCAC G[AGT]GC[ACT]^C 521 GGGCCC BsgI 1263 CTGCAC GTGCAG(16/14) 1334 GTGCAG Bsp1286I 74 GAGCAC G[AGT]GC[ACT]^C 521 GGGCCC DraII 520 GGGGCCC [GA]G^G[ACGT]CC[CT] 521 GGGCCCC EaeI 2 TGGCCG [CT]^GGCC[GA] 622 TGGCCA EcoO109I 520 GGGGCCC [GA]G^G[ACGT]CC[CT] 521 GGGCCCC MaeIII 943 GTCAC ^GT[ACGT]AC 1006 GTGAC MseI 119 TTAA T^TAA 932 TTAA SmlI 1019 CTCAAG C^T[CT][GA]AG 1474 CTCAAG TaqI 645 TCGA T^CGA 765 TCGA Tru9I 119 TTAA T^TAA 932 TTAA Tsp45I 943 GTCAC ^GT[GC]AC 1006 GTGAC TthHB8I 645 TCGA T^CGA 765 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI BbvI BcnI BfaI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII FauI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TfiI TseI Tsp509I TspRI XhoII