Restriction map for
pFN21AE4122
(Circular) (Six-base) MAPSORT of: pFN21AE4122.seq Check: 5274 from: 1 to: 6220
pFN21AE4122 6220 bp
With 182 enzymes: SgfI *
March 28, 2012 13:06 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2367 4030 278
Size: 53 83 186 936 831 1663 2468
Fragments arranged by size:
2468 1663 936 831 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3563 1937
Size: 1626 4594
Acc65I G'GTAC_C
Cuts at: 3541 3541
Size: 6220
AclI AA'CG_TT
Cuts at: 1563 4347 4720 1563
Size: 2784 373 3063
Fragments arranged by size:
3063 2784 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2177 3172 4283 5667 5935 1632
Size: 545 995 1111 1384 268 1917
Fragments arranged by size:
1917 1384 1111 995 545 268
AfeI AGC'GCT
Cuts at: 2044 2044
Size: 6220
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6023
AflIII A'CryG_T
Cuts at: 1203 3333 3486 5119 6096 1203
Size: 2130 153 1633 977 1327
Fragments arranged by size:
2130 1633 1327 977 153
AgeI A'CCGG_T
Cuts at: 5051 5051
Size: 6220
AhdI GACnn_n'nnGTC
Cuts at: 4949 4949
Size: 6220
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6220
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6220
AlwNI CAG_nnn'CTG
Cuts at: 3963 5535 3963
Size: 1572 4648
ApaI G_GGCC'C
Cuts at: 2530 2530
Size: 6220
ApaLI G'TGCA_C
Cuts at: 4277 5433 4277
Size: 1156 5064
ApoI r'AATT_y
Cuts at: 1493 2116 2567 2883 3529 3755 3907 6016 1493
Size: 623 451 316 646 226 152 2109 1697
Fragments arranged by size:
2109 1697 646 623 451 316 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2937 4774 160
Size: 872 1905 1837 1606
Fragments arranged by size:
1905 1837 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
AvaI C'yCGr_G
Cuts at: 1870 1943 3545 1870
Size: 73 1602 4545
Fragments arranged by size:
4545 1602 73
BamHI G'GATC_C
Cuts at: 3550 3917 3550
Size: 367 5853
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3541 4996 618
Size: 325 217 627 1754 1455 1842
Fragments arranged by size:
1842 1754 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2530 3539 1391
Size: 1139 1009 4072
Fragments arranged by size:
4072 1139 1009
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2959 928
Size: 971 1060 4189
Fragments arranged by size:
4189 1060 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4411 4445 1903
Size: 34 2474 34 3678
Fragments arranged by size:
3678 2474 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2785 4112 5322 2785
Size: 1327 1210 3683
Fragments arranged by size:
3683 1327 1210
BclI T'GATC_A
Cuts at: 1548 3351 1548
Size: 1803 4417
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4831 136
Size: 107 122 71 631 605 3159 1525
Fragments arranged by size:
3159 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2871 3369 6215 2871
Size: 498 2846 2876
Fragments arranged by size:
2876 2846 498
BlpI GC'TnA_GC
Cuts at: 2046 3637 2046
Size: 1591 4629
Bme1580I G_kGCm'C
Cuts at: 1790 2530 4281 5437 1790
Size: 740 1751 1156 2573
Fragments arranged by size:
2573 1751 1156 740
BmgBI CAC'GTC
Cuts at: 1318 3010 1318
Size: 1692 4528
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2341 3442 3951
Size: 416 53 83 399 24 924 1101 509
Cuts at: 3951 4909 442
Size: 958 1753
Fragments arranged by size:
1753 1101 958 924 509 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2158 2569 2683 4880 1239
Size: 919 411 114 2197 2579
Fragments arranged by size:
2579 2197 919 411 114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3009 3464 4345 5210 5508 3009
Size: 455 881 865 298 3721
Fragments arranged by size:
3721 881 865 455 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3401 4883 882
Size: 617 1902 1482 2219
Fragments arranged by size:
2219 1902 1482 617
BsaAI yAC'GTr
Cuts at: 493 1204 3334 4011 493
Size: 711 2130 677 2702
Fragments arranged by size:
2702 2130 711 677
BsaBI GATnn'nnATC
Cuts at: 6214 6214
Size: 6220
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2364 4027 4409 5044
Size: 53 83 186 936 831 1663 382 635
Cuts at: 5044 275
Size: 1451
Fragments arranged by size:
1663 1451 936 831 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4653 5051 5325 5472 5883 4653
Size: 398 274 147 411 4990
Fragments arranged by size:
4990 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2457 2487 1178
Size: 30 165 30 1054 30 4911
Fragments arranged by size:
4911 1054 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2053 2082 2716 3389 3395 1186
Size: 867 29 634 673 6 4011
Fragments arranged by size:
4011 867 673 634 29 6
BseYI C'CCAG_C
Cuts at: 2406 2686 5423 5919 2406
Size: 280 2737 496 2707
Fragments arranged by size:
2737 2707 496 280
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3253 3360 1163
Size: 573 1517 107 4023
Fragments arranged by size:
4023 1517 573 107
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3670 4431 5459 1540
Size: 275 186 1669 761 1028 2301
Fragments arranged by size:
2301 1669 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2083 4281 4366 5437 2083
Size: 2198 85 1071 2866
Fragments arranged by size:
2866 2198 1071 85
BsmI GAATG_Cn'
Cuts at: 2061 3731 3824 2061
Size: 1670 93 4457
Fragments arranged by size:
4457 1670 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2083 2530 3539 4281 4366 5437 1391
Size: 399 293 447 1009 742 85 1071 2174
Fragments arranged by size:
2174 1071 1009 742 447 399 293 85
BspEI T'CCGG_A
Cuts at: 5883 5883
Size: 6220
BspHI T'CATG_A
Cuts at: 2182 4109 5839 2182
Size: 1927 1730 2563
Fragments arranged by size:
2563 1927 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3163 3219 3249 3575 844
Size: 2319 56 30 326 3489
Fragments arranged by size:
3489 2319 326 56 30
BsrBI CCG'CTC
Cuts at: 1394 1823 4107 1394
Size: 429 2284 3507
Fragments arranged by size:
3507 2284 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4715 4889 65
Size: 1183 3467 174 1396
Fragments arranged by size:
3467 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4864 5051 1700
Size: 110 3054 187 2869
Fragments arranged by size:
3054 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2863 96
Size: 2767 3453
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6220
BssSI C'ACGA_G
Cuts at: 4280 5292 4280
Size: 1012 5208
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6220
BstBI TT'CG_AA
Cuts at: 5024 5024
Size: 6220
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6220
BstYI r'GATC_y
Cuts at: 1972 2502 2871 3369 3550 3917 4302 4319 5771
Size: 530 369 498 181 367 385 17 1452
Cuts at: 5771 5857 5869 6215 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 530 498 385 369 367 346
181 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2438 2438
Size: 6220
BtgI C'CryG_G
Cuts at: 513 1065 2004 2102 513
Size: 552 939 98 4631
Fragments arranged by size:
4631 939 552 98
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2238 487
Size: 683 56 1012 4469
Fragments arranged by size:
4469 1012 683 56
BtsI GCAGTG_nn'
Cuts at: 2404 3736 4530 4550 2404
Size: 1332 794 20 4074
Fragments arranged by size:
4074 1332 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6220
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6185
DraI TTT'AAA
Cuts at: 3524 3524
Size: 6220
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3019 5227 809
Size: 507 1703 2208 1802
Fragments arranged by size:
2208 1802 1703 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2627 3667
Size: 54 1425 50 275 18 177 620 1040
Cuts at: 3667 4556 6185 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1040 889 620 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3667 1537
Size: 275 1855 4090
Fragments arranged by size:
4090 1855 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2030 2229 2982 3141 3267 4150 1353
Size: 677 199 753 159 126 883 3423
Fragments arranged by size:
3423 883 753 677 199 159 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2894 3266 4807 5181 5327 387
Size: 1064 138 1305 372 1541 374 146 1280
Fragments arranged by size:
1541 1305 1280 1064 374 372 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2158 2177 2569 2683 3172 4283 4880
Size: 393 526 19 392 114 489 1111 597
Cuts at: 4880 5667 5935 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1111 787 597 526 489 393 392
268 114 19
EcoO109I rG'GnC_Cy
Cuts at: 2526 2527 2526
Size: 1 6219
EcoRI G'AATT_C
Cuts at: 2883 3529 3907 2883
Size: 646 378 5196
Fragments arranged by size:
5196 646 378
FspI TGC'GCA
Cuts at: 4726 4726
Size: 6220
HaeII r_GCGC'y
Cuts at: 1678 2046 5367 1678
Size: 368 3321 2531
Fragments arranged by size:
3321 2531 368
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2124 2156 2231 2263 2494
Size: 32 135 32 552 32 75 32 231
Cuts at: 2494 2526 2818 2850 4861 4893 4935 4967 1373
Size: 32 292 32 2011 32 42 32 2626
Fragments arranged by size:
2626 2011 552 292 231 135 75 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2143 2352 2712 3564 3810 669
Size: 988 281 205 209 360 852 246 3079
Fragments arranged by size:
3079 988 852 360 281 246 209 205
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6220
HpaI GTT'AAC
Cuts at: 3810 3810
Size: 6220
KpnI G_GTAC'C
Cuts at: 3545 3545
Size: 6220
MfeI C'AATT_G
Cuts at: 3819 3819
Size: 6220
MluI A'CGCG_T
Cuts at: 6096 6096
Size: 6220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5334 5518 1305
Size: 4029 184 2007
Fragments arranged by size:
4029 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2629 10
Size: 54 1425 343 797 3601
Fragments arranged by size:
3601 1425 797 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4178 4537 4696 518
Size: 648 3012 359 159 2042
Fragments arranged by size:
3012 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2012 2410 2722 3228 3636 4313 5461 5706
Size: 406 398 312 506 408 677 1148 245
Cuts at: 5706 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 506 408 406 398 312
245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4729
Fragments arranged by size:
4729 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6220
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6220
NotI GC'GGCC_GC
Cuts at: 3667 3667
Size: 6220
NspI r_CATG'y
Cuts at: 1132 1229 2436 3034 3490 3578 5123 1132
Size: 97 1207 598 456 88 1545 2229
Fragments arranged by size:
2229 1545 1207 598 456 97 88
PasI CC'CwG_GG
Cuts at: 1348 2640 1348
Size: 1292 4928
PciI A'CATG_T
Cuts at: 3486 5119 3486
Size: 1633 4587
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6220
PfoI T'CCnGG_A
Cuts at: 2544 3090 2544
Size: 546 5674
PmeI GTTT'AAAC
Cuts at: 3524 3524
Size: 6220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4289 4321 5802 5834 4289
Size: 32 1481 32 4675
Fragments arranged by size:
4675 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2671 1613
Size: 1058 5162
PsiI TTA'TAA
Cuts at: 3790 3790
Size: 6220
PspOMI G'GGCC_C
Cuts at: 2526 2526
Size: 6220
PstI C_TGCA'G
Cuts at: 830 2296 2721 3216 3572 830
Size: 1466 425 495 356 3478
Fragments arranged by size:
3478 1466 495 425 356
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6220
PvuII CAG'CTG
Cuts at: 2410 2410
Size: 6220
SalI G'TCGA_C
Cuts at: 1936 3562 1936
Size: 1626 4594
SapI GCTCTTCn'nnn_
Cuts at: 3141 3141
Size: 6220
SbfI CC_TGCA'GG
Cuts at: 3572 3572
Size: 6220
ScaI AGT'ACT
Cuts at: 4468 6180 4468
Size: 1712 4508
SexAI A'CCwGG_T
Cuts at: 2943 2943
Size: 6220
SfcI C'TryA_G
Cuts at: 826 1045 2292 2717 2797 3212 3410 3568 4703
Size: 219 1247 425 80 415 198 158 1135
Cuts at: 4703 5384 5575 826
Size: 681 191 1471
Fragments arranged by size:
1471 1247 1135 681 425 415 219 198
191 158 80
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6220
SmaI CCC'GGG
Cuts at: 1872 3547 1872
Size: 1675 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3024 3479 4324 5225 5487 820
Size: 197 926 1081 455 845 901 262 1553
Fragments arranged by size:
1553 1081 926 901 845 455 262 197
SnaBI TAC'GTA
Cuts at: 493 4011 493
Size: 3518 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6220
SphI G_CATG'C
Cuts at: 1132 3578 1132
Size: 2446 3774
SspI AAT'ATT
Cuts at: 5 52 2738 4144 5
Size: 47 2686 1406 2081
Fragments arranged by size:
2686 2081 1406 47
StuI AGG'CCT
Cuts at: 1517 2171 1517
Size: 654 5566
StyI C'CwwG_G
Cuts at: 513 1065 2004 2822 3659 513
Size: 552 939 818 837 3074
Fragments arranged by size:
3074 939 837 818 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4247 4264 4417 4602 5047 325
Size: 1255 2667 17 153 185 445 1498
Fragments arranged by size:
2667 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2863 3483 4466
Size: 274 80 33 51 1443 886 620 983
Cuts at: 4466 6178 96
Size: 1712 138
Fragments arranged by size:
1712 1443 983 886 620 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2188 2220 2241 2273 2457 2489 6180 6212 2188
Size: 32 21 32 184 32 3691 32 2196
Fragments arranged by size:
3691 2196 184 32 32 32 32 21
Tth111I GACn'n_nGTC
Cuts at: 1316 2945 1316
Size: 1629 4591
XbaI T'CTAG_A
Cuts at: 2305 3556 2305
Size: 1251 4969
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2915 2915
Size: 6220
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6220
XmaI C'CCGG_G
Cuts at: 1870 3545 1870
Size: 1675 4545
XmnI GAAnn'nnTTC
Cuts at: 2883 4349 2883
Size: 1466 4754
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2365 4028 276
Size: 53 83 186 936 831 1663 2468
Fragments arranged by size:
2468 1663 936 831 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI