(Linear) (Six-base) MAPSORT of: KIEE4122.seq Check: 4837 from: 1 to: 1515 KIEE4122.orf With 182 enzymes: SgfI * March 22, 2012 17:24 .. AatII G_ACGT'C Cuts at: 0 362 1515 Size: 362 1153 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 172 1167 1515 Size: 172 995 348 Fragments arranged by size: 995 348 172 AfeI AGC'GCT Cuts at: 0 39 1515 Size: 39 1476 AflIII A'CryG_T Cuts at: 0 1328 1481 1515 Size: 1328 153 34 Fragments arranged by size: 1328 153 34 ApaI G_GGCC'C Cuts at: 0 525 1515 Size: 525 990 ApoI r'AATT_y Cuts at: 0 111 562 878 1515 Size: 111 451 316 637 Fragments arranged by size: 637 451 316 111 AseI AT'TA_AT Cuts at: 0 932 1515 Size: 932 583 BanII G_rGCy'C Cuts at: 0 525 1515 Size: 525 990 BbsI GAAGACnn'nnnn_ Cuts at: 0 954 1515 Size: 954 561 BciVI GTATCCnnnnn_n' Cuts at: 0 780 1515 Size: 780 735 BclI T'GATC_A Cuts at: 0 1346 1515 Size: 1346 169 BglII A'GATC_T Cuts at: 0 866 1364 1515 Size: 866 498 151 Fragments arranged by size: 866 498 151 BlpI GC'TnA_GC Cuts at: 0 41 1515 Size: 41 1474 Bme1580I G_kGCm'C Cuts at: 0 525 1515 Size: 525 990 BmgBI CAC'GTC Cuts at: 0 1005 1515 Size: 1005 510 BmrI ACTGGGnnnn_n' Cuts at: 0 336 1437 1515 Size: 336 1101 78 Fragments arranged by size: 1101 336 78 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 153 564 678 1515 Size: 153 411 114 837 Fragments arranged by size: 837 411 153 114 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1004 1459 1515 Size: 1004 455 56 Fragments arranged by size: 1004 455 56 BsaI GGTCTCn'nnnn_ Cuts at: 0 1396 1515 Size: 1396 119 BsaAI yAC'GTr Cuts at: 0 1329 1515 Size: 1329 186 BsaHI Gr'CG_yC Cuts at: 0 359 1515 Size: 359 1156 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 452 482 1515 Size: 452 30 1033 Fragments arranged by size: 1033 452 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 48 77 711 1384 1390 1515 Size: 48 29 634 673 6 125 Fragments arranged by size: 673 634 125 48 29 6 BseYI C'CCAG_C Cuts at: 0 401 681 1515 Size: 401 280 834 Fragments arranged by size: 834 401 280 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1248 1355 1515 Size: 1248 107 160 Fragments arranged by size: 1248 160 107 BsiHKAI G_wGCw'C Cuts at: 0 78 1515 Size: 78 1437 BsmI GAATG_Cn' Cuts at: 0 56 1515 Size: 56 1459 Bsp1286I G_dGCh'C Cuts at: 0 78 525 1515 Size: 78 447 990 Fragments arranged by size: 990 447 78 BspHI T'CATG_A Cuts at: 0 177 1515 Size: 177 1338 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1158 1214 1244 1515 Size: 1158 56 30 271 Fragments arranged by size: 1158 271 56 30 BsrGI T'GTAC_A Cuts at: 0 858 1515 Size: 858 657 BstYI r'GATC_y Cuts at: 0 497 866 1364 1515 Size: 497 369 498 151 Fragments arranged by size: 498 497 369 151 Bsu36I CC'TnA_GG Cuts at: 0 433 1515 Size: 433 1082 BtgI C'CryG_G Cuts at: 0 97 1515 Size: 97 1418 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 233 1515 Size: 233 1282 BtsI GCAGTG_nn' Cuts at: 0 399 1515 Size: 399 1116 DrdI GACnn_nn'nnGTC Cuts at: 0 1014 1515 Size: 1014 501 EaeI y'GGCC_r Cuts at: 0 2 622 1515 Size: 2 620 893 Fragments arranged by size: 893 620 2 EarI CTCTTCn'nnn_ Cuts at: 0 25 224 977 1136 1262 1515 Size: 25 199 753 159 126 253 Fragments arranged by size: 753 253 199 159 126 25 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 889 1261 1515 Size: 889 372 254 Fragments arranged by size: 889 372 254 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 153 172 564 678 1167 1515 Size: 153 19 392 114 489 348 Fragments arranged by size: 489 392 348 153 114 19 EcoO109I rG'GnC_Cy Cuts at: 0 521 522 1515 Size: 521 1 993 Fragments arranged by size: 993 521 1 EcoRI G'AATT_C Cuts at: 0 878 1515 Size: 878 637 HaeII r_GCGC'y Cuts at: 0 41 1515 Size: 41 1474 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 119 151 226 258 489 521 813 845 Size: 119 32 75 32 231 32 292 32 Cuts at: 845 1515 Size: 670 Fragments arranged by size: 670 292 231 119 75 32 32 32 32 HincII GTy'rAC Cuts at: 0 138 347 707 1515 Size: 138 209 360 808 Fragments arranged by size: 808 360 209 138 MscI TGG'CCA Cuts at: 0 624 1515 Size: 624 891 MspA1I CmG'CkG Cuts at: 0 7 405 717 1223 1515 Size: 7 398 312 506 292 Fragments arranged by size: 506 398 312 292 7 NspI r_CATG'y Cuts at: 0 431 1029 1485 1515 Size: 431 598 456 30 Fragments arranged by size: 598 456 431 30 PasI CC'CwG_GG Cuts at: 0 635 1515 Size: 635 880 PciI A'CATG_T Cuts at: 0 1481 1515 Size: 1481 34 PfoI T'CCnGG_A Cuts at: 0 539 1085 1515 Size: 539 546 430 Fragments arranged by size: 546 539 430 PshAI GACnn'nnGTC Cuts at: 0 666 1515 Size: 666 849 PspOMI G'GGCC_C Cuts at: 0 521 1515 Size: 521 994 PstI C_TGCA'G Cuts at: 0 291 716 1211 1515 Size: 291 425 495 304 Fragments arranged by size: 495 425 304 291 PvuII CAG'CTG Cuts at: 0 405 1515 Size: 405 1110 SapI GCTCTTCn'nnn_ Cuts at: 0 1136 1515 Size: 1136 379 SexAI A'CCwGG_T Cuts at: 0 938 1515 Size: 938 577 SfcI C'TryA_G Cuts at: 0 287 712 792 1207 1405 1515 Size: 287 425 80 415 198 110 Fragments arranged by size: 425 415 287 198 110 80 SmlI C'TyrA_G Cuts at: 0 1019 1474 1515 Size: 1019 455 41 Fragments arranged by size: 1019 455 41 SspI AAT'ATT Cuts at: 0 733 1515 Size: 733 782 StuI AGG'CCT Cuts at: 0 166 1515 Size: 166 1349 StyI C'CwwG_G Cuts at: 0 817 1515 Size: 817 698 TatI w'GTAC_w Cuts at: 0 858 1478 1515 Size: 858 620 37 Fragments arranged by size: 858 620 37 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 183 215 236 268 452 484 1515 Size: 183 32 21 32 184 32 1031 Fragments arranged by size: 1031 184 183 32 32 32 21 Tth111I GACn'n_nGTC Cuts at: 0 940 1515 Size: 940 575 XbaI T'CTAG_A Cuts at: 0 300 1515 Size: 300 1215 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 910 1515 Size: 910 605 XmnI GAAnn'nnTTC Cuts at: 0 878 1515 Size: 878 637 ZraI GAC'GTC Cuts at: 0 360 1515 Size: 360 1155 Enzymes that do cut: AatII AcuI AfeI AflIII ApaI ApoI AseI BanII BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrGI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EarI EciI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII MscI MspA1I NspI PasI PciI PfoI PshAI PspOMI PstI PvuII SapI SexAI SfcI SmlI SspI StuI StyI TatI TstI Tth111I XbaI XcmI XmnI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BfrBI BglI BmtI BplI Bpu10I BsaBI BsaWI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I ClaI CspCI DraI DraIII EagI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI PmeI PmlI PpiI PpuMI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TaqII XhoI XmaI