(Circular) (Six-base) MAPSORT of: pFN21AE4079.seq Check: 511 from: 1 to: 5962 pFN21AE4079 5962 bp With 182 enzymes: SgfI * March 28, 2012 13:11 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3772 278 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3305 1937 Size: 1368 4594 Acc65I G'GTAC_C Cuts at: 3283 3283 Size: 5962 AclI AA'CG_TT Cuts at: 1563 4089 4462 1563 Size: 2526 373 3063 Fragments arranged by size: 3063 2526 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2186 2250 2526 3042 4025 5409 5677 1632 Size: 554 64 276 516 983 1384 268 1917 Fragments arranged by size: 1917 1384 983 554 516 276 268 64 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5765 AflIII A'CryG_T Cuts at: 1203 2381 4861 5838 1203 Size: 1178 2480 977 1327 Fragments arranged by size: 2480 1327 1178 977 AgeI A'CCGG_T Cuts at: 2555 4793 2555 Size: 2238 3724 AhdI GACnn_n'nnGTC Cuts at: 4691 4691 Size: 5962 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5962 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5962 AlwNI CAG_nnn'CTG Cuts at: 3705 5277 3705 Size: 1572 4390 ApaLI G'TGCA_C Cuts at: 4019 5175 4019 Size: 1156 4806 ApoI r'AATT_y Cuts at: 1493 2859 3271 3497 3649 5758 1493 Size: 1366 412 226 152 2109 1697 Fragments arranged by size: 2109 1697 1366 412 226 152 AseI AT'TA_AT Cuts at: 160 1032 2625 3179 4516 160 Size: 872 1593 554 1337 1606 Fragments arranged by size: 1606 1593 1337 872 554 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 AvaI C'yCGr_G Cuts at: 1870 1943 3287 1870 Size: 73 1344 4545 Fragments arranged by size: 4545 1344 73 BamHI G'GATC_C Cuts at: 3292 3659 3292 Size: 367 5595 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3283 4738 618 Size: 325 217 627 1496 1455 1842 Fragments arranged by size: 1842 1496 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2730 3281 1391 Size: 1339 551 4072 Fragments arranged by size: 4072 1339 551 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2169 2238 2829 928 Size: 971 270 69 591 4061 Fragments arranged by size: 4061 971 591 270 69 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4153 4187 1903 Size: 34 2216 34 3678 Fragments arranged by size: 3678 2216 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2416 3854 5064 2416 Size: 1438 1210 3314 Fragments arranged by size: 3314 1438 1210 BclI T'GATC_A Cuts at: 1548 2710 1548 Size: 1162 4800 BfrBI ATG'CAT Cuts at: 3184 3184 Size: 5962 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4573 136 Size: 107 122 71 631 605 2901 1525 Fragments arranged by size: 2901 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2593 2656 5957 2593 Size: 63 3301 2598 Fragments arranged by size: 3301 2598 63 BlpI GC'TnA_GC Cuts at: 3379 3379 Size: 5962 Bme1580I G_kGCm'C Cuts at: 1790 4023 5179 1790 Size: 2233 1156 2573 Fragments arranged by size: 2573 2233 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5962 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2824 3693 4651 Size: 416 53 83 399 24 1407 869 958 Cuts at: 4651 442 Size: 1753 Fragments arranged by size: 1753 1407 958 869 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5962 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2277 3168 4622 1239 Size: 1038 891 1454 2579 Fragments arranged by size: 2579 1454 1038 891 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4087 4952 5250 4087 Size: 865 298 4799 Fragments arranged by size: 4799 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4625 882 Size: 617 3126 2219 Fragments arranged by size: 3126 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3753 493 Size: 711 2549 2702 Fragments arranged by size: 2702 2549 711 BsaBI GATnn'nnATC Cuts at: 5956 5956 Size: 5962 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3769 4151 4786 275 Size: 53 83 186 936 2236 382 635 1451 Fragments arranged by size: 2236 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2419 2555 4395 4793 5067 5214 5625 2419 Size: 136 1840 398 274 147 411 2756 Fragments arranged by size: 2756 1840 411 398 274 147 136 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3141 3171 1178 Size: 30 165 30 1738 30 3969 Fragments arranged by size: 3969 1738 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5962 BseYI C'CCAG_C Cuts at: 2431 5165 5661 2431 Size: 2734 496 2732 Fragments arranged by size: 2734 2732 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5389 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3016 3412 4173 5201 1540 Size: 275 186 1015 396 761 1028 2301 Fragments arranged by size: 2301 1028 1015 761 396 275 186 BsiHKAI G_wGCw'C Cuts at: 2698 2730 4023 4108 5179 2698 Size: 32 1293 85 1071 3481 Fragments arranged by size: 3481 1293 1071 85 32 BsmI GAATG_Cn' Cuts at: 3473 3566 3473 Size: 93 5869 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2698 2730 3281 4023 4108 5179 1391 Size: 399 908 32 551 742 85 1071 2174 Fragments arranged by size: 2174 1071 908 742 551 399 85 32 BspEI T'CCGG_A Cuts at: 2419 5625 2419 Size: 3206 2756 BspHI T'CATG_A Cuts at: 3851 5581 3851 Size: 1730 4232 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3317 844 Size: 2473 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3849 1394 Size: 429 2026 3507 Fragments arranged by size: 3507 2026 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4457 4631 65 Size: 1183 3209 174 1396 Fragments arranged by size: 3209 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2555 3015 3213 4606 4793 1700 Size: 110 745 460 198 1393 187 2869 Fragments arranged by size: 2869 1393 745 460 198 187 110 BsrGI T'GTAC_A Cuts at: 96 3040 96 Size: 2944 3018 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5962 BssSI C'ACGA_G Cuts at: 4022 5034 4022 Size: 1012 4950 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5962 BstBI TT'CG_AA Cuts at: 3115 4766 3115 Size: 1651 4311 BstEII G'GTnAC_C Cuts at: 1184 2972 1184 Size: 1788 4174 BstXI CCAn_nnnn'nTGG Cuts at: 2746 2746 Size: 5962 BstYI r'GATC_y Cuts at: 1972 2593 2656 3292 3659 4044 4061 5513 5599 Size: 621 63 636 367 385 17 1452 86 Cuts at: 5599 5611 5957 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 636 621 385 367 346 86 63 17 12 BtgI C'CryG_G Cuts at: 513 1065 2017 513 Size: 552 952 4458 Fragments arranged by size: 4458 952 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5223 Fragments arranged by size: 5223 683 56 BtsI GCAGTG_nn' Cuts at: 2029 2409 3478 4272 4292 2029 Size: 380 1069 794 20 3699 Fragments arranged by size: 3699 1069 794 380 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5962 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5927 DraI TTT'AAA Cuts at: 3266 3266 Size: 5962 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4969 809 Size: 507 3653 1802 Fragments arranged by size: 3653 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3013 3409 4298 Size: 54 1425 50 275 18 1183 396 889 Cuts at: 4298 5927 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1183 889 396 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3013 3409 1537 Size: 275 1201 396 4090 Fragments arranged by size: 4090 1201 396 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2449 2718 3080 3892 1353 Size: 1096 269 362 812 3423 Fragments arranged by size: 3423 1096 812 362 269 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4549 4923 5069 387 Size: 1064 138 2960 374 146 1280 Fragments arranged by size: 2960 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2728 2728 Size: 5962 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2186 2250 2277 2526 3042 3168 4025 Size: 393 554 64 27 249 516 126 857 Cuts at: 4025 4622 5409 5677 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 857 787 597 554 516 393 268 249 126 64 27 EcoO109I rG'GnC_Cy Cuts at: 2150 2941 2150 Size: 791 5171 EcoRI G'AATT_C Cuts at: 3271 3649 3271 Size: 378 5584 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2381 2381 Size: 5962 FspI TGC'GCA Cuts at: 4468 4468 Size: 5962 HaeII r_GCGC'y Cuts at: 1678 5109 1678 Size: 3431 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2168 2200 2567 2599 3141 Size: 32 135 32 596 32 367 32 542 Cuts at: 3141 3173 4603 4635 4677 4709 1373 Size: 32 1430 32 42 32 2626 Fragments arranged by size: 2626 1430 596 542 367 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3306 3552 669 Size: 988 281 1368 246 3079 Fragments arranged by size: 3079 1368 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5962 HpaI GTT'AAC Cuts at: 3552 3552 Size: 5962 KpnI G_GTAC'C Cuts at: 3287 3287 Size: 5962 MfeI C'AATT_G Cuts at: 2566 3561 2566 Size: 995 4967 MluI A'CGCG_T Cuts at: 5838 5838 Size: 5962 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5076 5260 1305 Size: 3771 184 2007 Fragments arranged by size: 3771 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4140 Fragments arranged by size: 4140 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2744 2786 3059 3920 4279 4438 518 Size: 648 1578 42 273 861 359 159 2042 Fragments arranged by size: 2042 1578 861 648 359 273 159 42 MspA1I CmG'CkG Cuts at: 1606 2058 3378 4055 5203 5448 1606 Size: 452 1320 677 1148 245 2120 Fragments arranged by size: 2120 1320 1148 677 452 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5410 NdeI CA'TA_TG Cuts at: 387 387 Size: 5962 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5962 NotI GC'GGCC_GC Cuts at: 3409 3409 Size: 5962 NsiI A_TGCA'T Cuts at: 3186 3186 Size: 5962 NspI r_CATG'y Cuts at: 1132 1229 3320 4865 1132 Size: 97 2091 1545 2229 Fragments arranged by size: 2229 2091 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5962 PciI A'CATG_T Cuts at: 4861 4861 Size: 5962 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5962 PmeI GTTT'AAAC Cuts at: 3266 3266 Size: 5962 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4031 4063 5544 5576 4031 Size: 32 1481 32 4417 Fragments arranged by size: 4417 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2150 2150 Size: 5962 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5962 PsiI TTA'TAA Cuts at: 3532 3532 Size: 5962 PstI C_TGCA'G Cuts at: 830 3314 830 Size: 2484 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5962 PvuII CAG'CTG Cuts at: 2058 2058 Size: 5962 SacI G_AGCT'C Cuts at: 2730 2730 Size: 5962 SalI G'TCGA_C Cuts at: 1936 3304 1936 Size: 1368 4594 SapI GCTCTTCn'nnn_ Cuts at: 2718 2718 Size: 5962 SbfI CC_TGCA'GG Cuts at: 3314 3314 Size: 5962 ScaI AGT'ACT Cuts at: 4210 5922 4210 Size: 1712 4250 SfcI C'TryA_G Cuts at: 826 1045 2082 2881 3310 4445 5126 5317 826 Size: 219 1037 799 429 1135 681 191 1471 Fragments arranged by size: 1471 1135 1037 799 681 429 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5962 SmaI CCC'GGG Cuts at: 1872 3289 1872 Size: 1417 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4066 4967 5229 820 Size: 197 926 2123 901 262 1553 Fragments arranged by size: 2123 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3753 493 Size: 3260 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5962 SphI G_CATG'C Cuts at: 1132 3320 1132 Size: 2188 3774 SspI AAT'ATT Cuts at: 5 52 2852 3886 5 Size: 47 2800 1034 2081 Fragments arranged by size: 2800 2081 1034 47 StuI AGG'CCT Cuts at: 1517 3007 1517 Size: 1490 4472 StyI C'CwwG_G Cuts at: 513 1065 3401 513 Size: 552 2336 3074 Fragments arranged by size: 3074 2336 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3204 3989 4006 4159 4344 4789 325 Size: 1255 1624 785 17 153 185 445 1498 Fragments arranged by size: 1624 1498 1255 785 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2460 3040 4208 Size: 274 80 33 51 1443 483 580 1168 Cuts at: 4208 5920 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1168 580 483 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2401 2433 3092 3124 5922 5954 2401 Size: 32 659 32 2798 32 2409 Fragments arranged by size: 2798 2409 659 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5962 XbaI T'CTAG_A Cuts at: 3298 3298 Size: 5962 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2062 2062 Size: 5962 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5962 XmaI C'CCGG_G Cuts at: 1870 3287 1870 Size: 1417 4545 XmnI GAAnn'nnTTC Cuts at: 4091 4091 Size: 5962 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3770 276 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI