Restriction map for
pFN21AE4079
(Linear) (Six-base) MAPSORT of: KIEE4079.seq Check: 434 from: 1 to: 1257
KIEE4079.orf
With 182 enzymes: SgfI *
March 22, 2012 17:20 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 245 521 1037 1257
Size: 181 64 276 516 220
Fragments arranged by size:
516 276 220 181 64
AflIII A'CryG_T
Cuts at: 0 376 1257
Size: 376 881
AgeI A'CCGG_T
Cuts at: 0 550 1257
Size: 550 707
ApoI r'AATT_y
Cuts at: 0 854 1257
Size: 854 403
AseI AT'TA_AT
Cuts at: 0 620 1174 1257
Size: 620 554 83
Fragments arranged by size:
620 554 83
BanII G_rGCy'C
Cuts at: 0 725 1257
Size: 725 532
BbsI GAAGACnn'nnnn_
Cuts at: 0 164 233 824 1257
Size: 164 69 591 433
Fragments arranged by size:
591 433 164 69
BciVI GTATCCnnnnn_n'
Cuts at: 0 411 1257
Size: 411 846
BclI T'GATC_A
Cuts at: 0 705 1257
Size: 705 552
BfrBI ATG'CAT
Cuts at: 0 1179 1257
Size: 1179 78
BglII A'GATC_T
Cuts at: 0 588 651 1257
Size: 588 63 606
Fragments arranged by size:
606 588 63
BmrI ACTGGGnnnn_n'
Cuts at: 0 819 1257
Size: 819 438
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 1163 1257
Size: 272 891 94
Fragments arranged by size:
891 272 94
BsaWI w'CCGG_w
Cuts at: 0 414 550 1257
Size: 414 136 707
Fragments arranged by size:
707 414 136
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1136 1166 1257
Size: 1136 30 91
Fragments arranged by size:
1136 91 30
BseYI C'CCAG_C
Cuts at: 0 426 1257
Size: 426 831
BsiEI CG_ry'CG
Cuts at: 0 1011 1257
Size: 1011 246
BsiHKAI G_wGCw'C
Cuts at: 0 693 725 1257
Size: 693 32 532
Fragments arranged by size:
693 532 32
Bsp1286I G_dGCh'C
Cuts at: 0 693 725 1257
Size: 693 32 532
Fragments arranged by size:
693 532 32
BspEI T'CCGG_A
Cuts at: 0 414 1257
Size: 414 843
BsrFI r'CCGG_y
Cuts at: 0 550 1010 1208 1257
Size: 550 460 198 49
Fragments arranged by size:
550 460 198 49
BsrGI T'GTAC_A
Cuts at: 0 1035 1257
Size: 1035 222
BstBI TT'CG_AA
Cuts at: 0 1110 1257
Size: 1110 147
BstEII G'GTnAC_C
Cuts at: 0 967 1257
Size: 967 290
BstXI CCAn_nnnn'nTGG
Cuts at: 0 741 1257
Size: 741 516
BstYI r'GATC_y
Cuts at: 0 588 651 1257
Size: 588 63 606
Fragments arranged by size:
606 588 63
BtgI C'CryG_G
Cuts at: 0 12 1257
Size: 12 1245
BtsI GCAGTG_nn'
Cuts at: 0 24 404 1257
Size: 24 380 853
Fragments arranged by size:
853 380 24
EaeI y'GGCC_r
Cuts at: 0 1008 1257
Size: 1008 249
EagI C'GGCC_G
Cuts at: 0 1008 1257
Size: 1008 249
EarI CTCTTCn'nnn_
Cuts at: 0 444 713 1075 1257
Size: 444 269 362 182
Fragments arranged by size:
444 362 269 182
EcoICRI GAG'CTC
Cuts at: 0 723 1257
Size: 723 534
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 245 272 521 1037 1163 1257
Size: 181 64 27 249 516 126 94
Fragments arranged by size:
516 249 181 126 94 64 27
EcoO109I rG'GnC_Cy
Cuts at: 0 145 936 1257
Size: 145 791 321
Fragments arranged by size:
791 321 145
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 376 1257
Size: 376 881
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 163 195 562 594 1136 1168 1257
Size: 163 32 367 32 542 32 89
Fragments arranged by size:
542 367 163 89 32 32 32
MfeI C'AATT_G
Cuts at: 0 561 1257
Size: 561 696
MslI CAynn'nnrTG
Cuts at: 0 739 781 1054 1257
Size: 739 42 273 203
Fragments arranged by size:
739 273 203 42
MspA1I CmG'CkG
Cuts at: 0 53 1257
Size: 53 1204
NsiI A_TGCA'T
Cuts at: 0 1181 1257
Size: 1181 76
PpuMI rG'GwC_Cy
Cuts at: 0 145 1257
Size: 145 1112
PvuII CAG'CTG
Cuts at: 0 53 1257
Size: 53 1204
SacI G_AGCT'C
Cuts at: 0 725 1257
Size: 725 532
SapI GCTCTTCn'nnn_
Cuts at: 0 713 1257
Size: 713 544
SfcI C'TryA_G
Cuts at: 0 77 876 1257
Size: 77 799 381
Fragments arranged by size:
799 381 77
SspI AAT'ATT
Cuts at: 0 847 1257
Size: 847 410
StuI AGG'CCT
Cuts at: 0 1002 1257
Size: 1002 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1199 1257
Size: 1199 58
TatI w'GTAC_w
Cuts at: 0 455 1035 1257
Size: 455 580 222
Fragments arranged by size:
580 455 222
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 396 428 1087 1119 1257
Size: 396 32 659 32 138
Fragments arranged by size:
659 396 138 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 57 1257
Size: 57 1200
Enzymes that do cut:
AcuI AflIII AgeI ApoI AseI BanII BbsI BciVI
BclI BfrBI BglII BmrI BpmI BsaWI BsaXI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BsrFI BsrGI BstBI BstEII
BstXI BstYI BtgI BtsI EaeI EagI EarI EcoICRI
Eco57MI EcoO109I FalI Hin4I MfeI MslI MspA1I NsiI
PpuMI PvuII SacI SapI SfcI SspI StuI TaqII
TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BglI BlpI Bme1580I BmgBI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BseRI BsgI BsiWI BsmI
BsmBI BspHI BspMI BsrBI BsrDI BssHII BssSI BstAPI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EciI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI StyI SwaI Tth111I XbaI XhoI XmaI XmnI
ZraI