Restriction map for
pFN21AE5815
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccII AccIII AflII
AflIII AgeI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AvaI AviII AvrII
BbeI BbrPI BcgI BciVI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BsgI BsiEI
BsiWI BsmI BsoBI Bsp1407I BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI Bsu36I CelII Cfr10I
CpoI DraI DraII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV FauI FseI
FspI HapII HindIII HpaII KasI
KpnI KspI MamI MfeI MluI
MroI MslI MspI MunI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 85 GTATAC GT^[AC][GT]AC
AclI 478 AACGTT AA^CGTT
AcsI 1451 AAATTT [GA]^AATT[CT]
AfaI 1876 GTAC GT^AC
AhdI 170 GACACCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 1451 AAATTT [GA]^AATT[CT]
AspEI 170 GACACCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2523 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 665 TGGCCA TGG^CCA
BamHI 1581 GGATCC G^GATCC
BanI 1431 GGTGCC G^G[CT][GA]CC
BclI 473 TGATCA T^GATCA
BglII 904 AGATCT A^GATCT
BsaAI 1882 TACGTG [CT]AC^GT[GA]
Bst1107I 85 GTATAC GTA^TAC
BstXI 1853 CCATCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 85 GTATAC GTA^TAC
DraIII 1879 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1871 CCATGG C^C[GA][CT]GG
EaeI 665 TGGCCA [CT]^GGCC[GA]
Eam1105I 170 GACACCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2069 CCTTGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 213 ATGCAT ATGCA^T
FbaI 473 TGATCA T^GATCA
HaeII 19 AGCGCC [GA]GCGC^[CT]
HpaI 1600 GTTAAC GTT^AAC
MluNI 665 TGGCCA TGG^CCA
MscI 665 TGGCCA TGG^CCA
NcoI 1871 CCATGG C^CATGG
NdeI 65 CATATG CA^TATG
NsiI 213 ATGCAT ATGCA^T
NspI 215 GCATGC [GA]CATG^[CT]
PflFI 2523 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 478 AACGTT AA^CGTT
PstI 1359 CTGCAG CTGCA^G
PvuII 2032 CAGCTG CAG^CTG
RsaI 1876 GTAC GT^AC
SmlI 582 CTTGAG C^T[CT][GA]AG
SphI 215 GCATGC GCATG^C
Tth111I 2523 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 945 CCAGGATAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
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AcyI 2053 GACGCC G[GA]^CG[CT]C
2543 GACGCC
Asp700I 1344 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1998 GAATGATTTC
AvaII 1131 GGACC G^G[AT]CC
2307 GGACC
BbvCI 2040 CCTCAGC CCTCAGC(-5/-2)
2455 CCTCAGC
BsaHI 2053 GACGCC G[GA]^CG[CT]C
2543 GACGCC
BsmBI 1829 CGTCTC CGTCTC(1/5)
2541 GAGACG
BsmFI 1023 GGGAC GGGAC(10/14)
1185 GGGAC
BspDI 257 ATCGAT AT^CGAT
2107 ATCGAT
BspMI 991 GCAGGT ACCTGC(4/8)
1505 GCAGGT
CfoI 20 GCGC GCG^C
989 GCGC
ClaI 257 ATCGAT AT^CGAT
2107 ATCGAT
HhaI 20 GCGC GCG^C
989 GCGC
Hin1I 2053 GACGCC G[GA]^CG[CT]C
2543 GACGCC
HinP1I 20 GCGC G^CGC
989 GCGC
HincII 509 GTCAAC GT[CT]^[GA]AC
1600 GTTAAC
HindII 509 GTCAAC GT[CT]^[GA]AC
1600 GTTAAC
PflMI 1115 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2547 CCATCCTATGG
SacI 786 GAGCTC GAGCT^C
2441 GAGCTC
SfcI 1359 CTGCAG C^T[GA][CT]AG
2243 CTGTAG
StuI 125 AGGCCT AGG^CCT
327 AGGCCT
TaqI 258 TCGA T^CGA
2108 TCGA
Tsp45I 1475 GTGAC ^GT[GC]AC
1967 GTCAC
TthHB8I 258 TCGA T^CGA
2108 TCGA
Van91I 1115 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2547 CCATCCTATGG
XmnI 1344 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1998 GAATGATTTC
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# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AluI AlwI AspHI BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HgaI HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI StyI
TfiI Tru9I TseI Tsp509I TspRI
XhoII