Restriction map for
pFN21AE5815
(Circular) (Six-base) MAPSORT of: pFN21AE5815.seq Check: 198 from: 1 to: 7300
pFN21AE5815 7300 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2987 3501 2987
Size: 514 6786
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5110 278
Size: 53 83 186 936 3574 2468
Fragments arranged by size:
3574 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2091 4643 1937
Size: 154 2552 4594
Fragments arranged by size:
4594 2552 154
Acc65I G'GTAC_C
Cuts at: 4621 4621
Size: 7300
AclI AA'CG_TT
Cuts at: 1563 2484 5427 5800 1563
Size: 921 2943 373 3063
Fragments arranged by size:
3063 2943 921 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2023 2155 5363 6747 7015 1632
Size: 391 132 3208 1384 268 1917
Fragments arranged by size:
3208 1917 1384 391 268 132
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7103
AflIII A'CryG_T
Cuts at: 1203 6199 7176 1203
Size: 4996 977 1327
Fragments arranged by size:
4996 1327 977
AgeI A'CCGG_T
Cuts at: 6131 6131
Size: 7300
AhdI GACnn_n'nnGTC
Cuts at: 2180 6029 2180
Size: 3849 3451
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7300
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7300
AlwNI CAG_nnn'CTG
Cuts at: 5043 6615 5043
Size: 1572 5728
ApaLI G'TGCA_C
Cuts at: 5357 6513 5357
Size: 1156 6144
ApoI r'AATT_y
Cuts at: 1493 3456 4609 4835 4987 7096 1493
Size: 1963 1153 226 152 2109 1697
Fragments arranged by size:
2109 1963 1697 1153 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5854 160
Size: 872 4822 1606
Fragments arranged by size:
4822 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7300
AvaI C'yCGr_G
Cuts at: 1870 1943 4625 1870
Size: 73 2682 4545
Fragments arranged by size:
4545 2682 73
BamHI G'GATC_C
Cuts at: 3586 4630 4997 3586
Size: 1044 367 5889
Fragments arranged by size:
5889 1044 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3436 4621 6076 618
Size: 325 217 627 1649 1185 1455 1842
Fragments arranged by size:
1842 1649 1455 1185 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2501 2795 4353 4395 4425 4450 4619 1391
Size: 1110 294 1558 42 30 25 169 4072
Fragments arranged by size:
4072 1558 1110 294 169 42 30 25
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2825 2974 3669 928
Size: 971 926 149 695 4559
Fragments arranged by size:
4559 971 926 695 149
BbvCI CC'TCA_GC
Cuts at: 4046 4461 4046
Size: 415 6885
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5491 5525 1903
Size: 34 3554 34 3678
Fragments arranged by size:
3678 3554 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5192 6402 5192
Size: 1210 6090
BclI T'GATC_A
Cuts at: 1548 2478 1548
Size: 930 6370
BfrBI ATG'CAT
Cuts at: 2220 2220
Size: 7300
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5911 136
Size: 107 122 71 631 605 4239 1525
Fragments arranged by size:
4239 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2909 7295 2909
Size: 4386 2914
BlpI GC'TnA_GC
Cuts at: 4717 4717
Size: 7300
Bme1580I G_kGCm'C
Cuts at: 1790 5361 6517 1790
Size: 3571 1156 2573
Fragments arranged by size:
3571 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2182 1318
Size: 864 6436
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2628 3840 4501
Size: 416 53 83 399 24 1211 1212 661
Cuts at: 4501 5031 5989 442
Size: 530 958 1753
Fragments arranged by size:
1753 1212 1211 958 661 530 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7300
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2982 3557 4355 5960 1239
Size: 1743 575 798 1605 2579
Fragments arranged by size:
2579 1743 1605 798 575
Bpu10I CC'TnA_GC
Cuts at: 4046 4461 4046
Size: 415 6885
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2608 5425 6290 6588 2608
Size: 2817 865 298 3320
Fragments arranged by size:
3320 2817 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2096 4355 4361 5963 882
Size: 617 597 2259 6 1602 2219
Fragments arranged by size:
2259 2219 1602 617 597 6
BsaAI yAC'GTr
Cuts at: 493 1204 3889 5091 493
Size: 711 2685 1202 2702
Fragments arranged by size:
2702 2685 1202 711
BsaBI GATnn'nnATC
Cuts at: 7294 7294
Size: 7300
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4059 4549 5107 5489
Size: 53 83 186 936 2526 490 558 382
Cuts at: 5489 6124 275
Size: 635 1451
Fragments arranged by size:
2526 1451 936 635 558 490 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5733 6131 6405 6552 6963 5733
Size: 398 274 147 411 6070
Fragments arranged by size:
6070 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4250 4280 1178
Size: 30 165 30 2847 30 4198
Fragments arranged by size:
4198 2847 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2035 2114 2125 2361 3737 3820 4087 4271
Size: 849 79 11 236 1376 83 267 184
Cuts at: 4271 4558 1186
Size: 287 3928
Fragments arranged by size:
3928 1376 849 287 267 236 184 83
79 11
BseYI C'CCAG_C
Cuts at: 3119 4035 4298 4303 4395 6503 6999 3119
Size: 916 263 5 92 2108 496 3420
Fragments arranged by size:
3420 2108 916 496 263 92 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6727
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4750 5511 6539 1540
Size: 275 186 2749 761 1028 2301
Fragments arranged by size:
2749 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2473 2795 3154 4450 5361 5446 6517 2473
Size: 322 359 1296 911 85 1071 3256
Fragments arranged by size:
3256 1296 1071 911 359 322 85
BsmI GAATG_Cn'
Cuts at: 4811 4904 4811
Size: 93 7207
BsmBI CGTCTCn'nnnn_
Cuts at: 3840 4540 3840
Size: 700 6600
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2473 2501 2795 3154 4353 4395 4425
Size: 399 683 28 294 359 1199 42 30
Cuts at: 4425 4450 4619 5361 5446 6517 1391
Size: 25 169 742 85 1071 2174
Fragments arranged by size:
2174 1199 1071 742 683 399 359 294
169 85 42 30 28 25
BspEI T'CCGG_A
Cuts at: 6963 6963
Size: 7300
BspHI T'CATG_A
Cuts at: 5189 6919 5189
Size: 1730 5570
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2987 3501 4655 844
Size: 2143 514 1154 3489
Fragments arranged by size:
3489 2143 1154 514
BsrBI CCG'CTC
Cuts at: 1394 1823 5187 1394
Size: 429 3364 3507
Fragments arranged by size:
3507 3364 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2893 3934 3960 4594 5795 5969 65
Size: 1183 1645 1041 26 634 1201 174 1396
Fragments arranged by size:
1645 1396 1201 1183 1041 634 174 26
BsrFI r'CCGG_y
Cuts at: 1700 1810 5944 6131 1700
Size: 110 4134 187 2869
Fragments arranged by size:
4134 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7300
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7300
BssSI C'ACGA_G
Cuts at: 5360 6372 5360
Size: 1012 6288
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7300
BstBI TT'CG_AA
Cuts at: 6104 6104
Size: 7300
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7300
BstXI CCAn_nnnn'nTGG
Cuts at: 3865 3865
Size: 7300
BstYI r'GATC_y
Cuts at: 1972 2528 2909 3586 4236 4630 4997 5382 5399
Size: 556 381 677 650 394 367 385 17
Cuts at: 5399 6851 6937 6949 7295 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 677 650 556 394 385 381
367 346 86 17 12
BstZ17I GTA'TAC
Cuts at: 2092 2092
Size: 7300
BtgI C'CryG_G
Cuts at: 513 1065 2004 3876 513
Size: 552 939 1872 3937
Fragments arranged by size:
3937 1872 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2365 487
Size: 683 56 1139 5422
Fragments arranged by size:
5422 1139 683 56
BtsI GCAGTG_nn'
Cuts at: 3044 3616 4816 5610 5630 3044
Size: 572 1200 794 20 4714
Fragments arranged by size:
4714 1200 794 572 20
ClaI AT'CG_AT
Cuts at: 1554 2263 4113 1554
Size: 709 1850 4741
Fragments arranged by size:
4741 1850 709
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7265
DraI TTT'AAA
Cuts at: 4604 4604
Size: 7300
DraIII CAC_nnn'GTG
Cuts at: 3889 3889
Size: 7300
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6307 809
Size: 507 4991 1802
Fragments arranged by size:
4991 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2670 4747 5636
Size: 54 1425 50 275 18 840 2077 889
Cuts at: 5636 7265 8
Size: 1629 43
Fragments arranged by size:
2077 1629 1425 889 840 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4747 1537
Size: 275 2935 4090
Fragments arranged by size:
4090 2935 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2143 3982 4248 5230 1353
Size: 790 1839 266 982 3423
Fragments arranged by size:
3423 1839 982 790 266
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3806 5887 6261 6407 387
Size: 1064 138 2217 2081 374 146 1280
Fragments arranged by size:
2217 2081 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2793 4448 2793
Size: 1655 5645
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2023 2155 2982 3557 4355 5363 5960
Size: 393 391 132 827 575 798 1008 597
Cuts at: 5960 6747 7015 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1008 827 798 787 597 575 393
391 268 132
EcoNI CCTnn'n_nnAGG
Cuts at: 4078 4078
Size: 7300
EcoRI G'AATT_C
Cuts at: 4609 4987 4609
Size: 378 6922
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3758 3758
Size: 7300
FspI TGC'GCA
Cuts at: 5806 5806
Size: 7300
HaeII r_GCGC'y
Cuts at: 1678 2028 6447 1678
Size: 350 4419 2531
Fragments arranged by size:
4419 2531 350
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2166 2198 2356 2388 2520
Size: 32 135 32 594 32 158 32 132
Cuts at: 2520 2552 2815 2847 3195 3227 3801 3833 4268
Size: 32 263 32 348 32 574 32 435
Cuts at: 4268 4300 4507 4537 4539 4553 4569 4585 5941
Size: 32 207 30 2 14 16 16 1356
Cuts at: 5941 5973 6015 6047 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1356 594 574 435 348 263 207
158 135 132 42 32 32 32 32
32 32 32 32 32 32 32 30
16 16 14 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2516 3607 4644 4890 669
Size: 988 281 578 1091 1037 246 3079
Fragments arranged by size:
3079 1091 1037 988 578 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7300
HpaI GTT'AAC
Cuts at: 3607 4890 3607
Size: 1283 6017
KpnI G_GTAC'C
Cuts at: 4625 4625
Size: 7300
MfeI C'AATT_G
Cuts at: 4899 4899
Size: 7300
MluI A'CGCG_T
Cuts at: 7176 7176
Size: 7300
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2880 4242 4248 4264 6414 6598 1305
Size: 1575 1362 6 16 2150 184 2007
Fragments arranged by size:
2150 2007 1575 1362 184 16 6
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2672 10
Size: 54 1425 343 840 4638
Fragments arranged by size:
4638 1425 840 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5258 5617 5776 518
Size: 648 4092 359 159 2042
Fragments arranged by size:
4092 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2193 3544 4039 4716 5393 6541 6786 1606
Size: 587 1351 495 677 677 1148 245 2120
Fragments arranged by size:
2120 1351 1148 677 677 587 495 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3876 513
Size: 552 939 1872 3937
Fragments arranged by size:
3937 1872 939 552
NdeI CA'TA_TG
Cuts at: 387 2071 387
Size: 1684 5616
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7300
NotI GC'GGCC_GC
Cuts at: 4747 4747
Size: 7300
NsiI A_TGCA'T
Cuts at: 2222 2222
Size: 7300
NspI r_CATG'y
Cuts at: 1132 1229 2224 4658 6203 1132
Size: 97 995 2434 1545 2229
Fragments arranged by size:
2434 2229 1545 995 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7300
PciI A'CATG_T
Cuts at: 6199 6199
Size: 7300
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3126 4558 1108
Size: 2018 1432 3850
Fragments arranged by size:
3850 2018 1432
PfoI T'CCnGG_A
Cuts at: 2949 3864 2949
Size: 915 6385
PmeI GTTT'AAAC
Cuts at: 4604 4604
Size: 7300
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5369 5401 6882 6914 5369
Size: 32 1481 32 5755
Fragments arranged by size:
5755 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7300
PsiI TTA'TAA
Cuts at: 4870 4870
Size: 7300
PstI C_TGCA'G
Cuts at: 830 3368 4652 830
Size: 2538 1284 3478
Fragments arranged by size:
3478 2538 1284
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7300
PvuII CAG'CTG
Cuts at: 4039 4039
Size: 7300
SacI G_AGCT'C
Cuts at: 2795 4450 2795
Size: 1655 5645
SalI G'TCGA_C
Cuts at: 1936 4642 1936
Size: 2706 4594
SbfI CC_TGCA'GG
Cuts at: 4652 4652
Size: 7300
ScaI AGT'ACT
Cuts at: 5548 7260 5548
Size: 1712 5588
SfcI C'TryA_G
Cuts at: 826 1045 3364 4248 4648 5783 6464 6655 826
Size: 219 2319 884 400 1135 681 191 1471
Fragments arranged by size:
2319 1471 1135 884 681 400 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7300
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7300
SmaI CCC'GGG
Cuts at: 1872 4627 1872
Size: 2755 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2587 5404 6305 6567 820
Size: 197 926 644 2817 901 262 1553
Fragments arranged by size:
2817 1553 926 901 644 262 197
SnaBI TAC'GTA
Cuts at: 493 5091 493
Size: 4598 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7300
SphI G_CATG'C
Cuts at: 1132 2224 4658 1132
Size: 1092 2434 3774
Fragments arranged by size:
3774 2434 1092
SspI AAT'ATT
Cuts at: 5 52 5224 5
Size: 47 5172 2081
Fragments arranged by size:
5172 2081 47
StuI AGG'CCT
Cuts at: 1517 2132 2334 1517
Size: 615 202 6483
Fragments arranged by size:
6483 615 202
StyI C'CwwG_G
Cuts at: 513 1065 2004 2329 3876 4353 4739 513
Size: 552 939 325 1547 477 386 3074
Fragments arranged by size:
3074 1547 939 552 477 386 325
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4379 4397 4520 5327 5344 5497 5682
Size: 1255 2799 18 123 807 17 153 185
Cuts at: 5682 6127 325
Size: 445 1498
Fragments arranged by size:
2799 1498 1255 807 445 185 153 123
18 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5546 7258 96
Size: 274 80 33 51 1443 3569 1712 138
Fragments arranged by size:
3569 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7260 7292 7260
Size: 32 7268
Tth111I GACn'n_nGTC
Cuts at: 1316 4531 1316
Size: 3215 4085
XbaI T'CTAG_A
Cuts at: 4636 4636
Size: 7300
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2957 2957
Size: 7300
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7300
XmaI C'CCGG_G
Cuts at: 1870 4625 1870
Size: 2755 4545
XmnI GAAnn'nnTTC
Cuts at: 3353 4007 5429 3353
Size: 654 1422 5224
Fragments arranged by size:
5224 1422 654
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5108 276
Size: 53 83 186 936 3574 2468
Fragments arranged by size:
3574 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI Bsu36I EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI