Restriction map for
pFN21AE5815
(Linear) (Six-base) MAPSORT of: KIEE5815.seq Check: 8788 from: 1 to: 2595
KIEE5815.orf
With 182 enzymes: SgfI *
December 5, 2014 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 982 1496 2595
Size: 982 514 1099
Fragments arranged by size:
1099 982 514
AccI GT'mk_AC
Cuts at: 0 86 2595
Size: 86 2509
AclI AA'CG_TT
Cuts at: 0 479 2595
Size: 479 2116
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 150 2595
Size: 18 132 2445
Fragments arranged by size:
2445 132 18
AhdI GACnn_n'nnGTC
Cuts at: 0 175 2595
Size: 175 2420
ApoI r'AATT_y
Cuts at: 0 1451 2595
Size: 1451 1144
BamHI G'GATC_C
Cuts at: 0 1581 2595
Size: 1581 1014
BanI G'GyrC_C
Cuts at: 0 1431 2595
Size: 1431 1164
BanII G_rGCy'C
Cuts at: 0 496 790 2348 2390 2420 2445 2595
Size: 496 294 1558 42 30 25 150
Fragments arranged by size:
1558 496 294 150 42 30 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 820 969 1664 2595
Size: 820 149 695 931
Fragments arranged by size:
931 820 695 149
BbvCI CC'TCA_GC
Cuts at: 0 2041 2456 2595
Size: 2041 415 139
Fragments arranged by size:
2041 415 139
BclI T'GATC_A
Cuts at: 0 473 2595
Size: 473 2122
BfrBI ATG'CAT
Cuts at: 0 215 2595
Size: 215 2380
BglII A'GATC_T
Cuts at: 0 904 2595
Size: 904 1691
BmgBI CAC'GTC
Cuts at: 0 177 2595
Size: 177 2418
BmrI ACTGGGnnnn_n'
Cuts at: 0 623 1835 2496 2595
Size: 623 1212 661 99
Fragments arranged by size:
1212 661 623 99
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 977 1552 2350 2595
Size: 977 575 798 245
Fragments arranged by size:
977 798 575 245
Bpu10I CC'TnA_GC
Cuts at: 0 2041 2456 2595
Size: 2041 415 139
Fragments arranged by size:
2041 415 139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 603 2595
Size: 603 1992
BsaI GGTCTCn'nnnn_
Cuts at: 0 91 2350 2356 2595
Size: 91 2259 6 239
Fragments arranged by size:
2259 239 91 6
BsaAI yAC'GTr
Cuts at: 0 1884 2595
Size: 1884 711
BsaHI Gr'CG_yC
Cuts at: 0 2054 2544 2595
Size: 2054 490 51
Fragments arranged by size:
2054 490 51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2245 2275 2595
Size: 2245 30 320
Fragments arranged by size:
2245 320 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 30 109 120 356 1732 1815 2082 2266
Size: 30 79 11 236 1376 83 267 184
Cuts at: 2266 2553 2595
Size: 287 42
Fragments arranged by size:
1376 287 267 236 184 83 79 42
30 11
BseYI C'CCAG_C
Cuts at: 0 1114 2030 2293 2298 2390 2595
Size: 1114 916 263 5 92 205
Fragments arranged by size:
1114 916 263 205 92 5
BsiHKAI G_wGCw'C
Cuts at: 0 468 790 1149 2445 2595
Size: 468 322 359 1296 150
Fragments arranged by size:
1296 468 359 322 150
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1835 2535 2595
Size: 1835 700 60
Fragments arranged by size:
1835 700 60
Bsp1286I G_dGCh'C
Cuts at: 0 468 496 790 1149 2348 2390 2420 2445
Size: 468 28 294 359 1199 42 30 25
Cuts at: 2445 2595
Size: 150
Fragments arranged by size:
1199 468 359 294 150 42 30 28
25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 982 1496 2595
Size: 982 514 1099
Fragments arranged by size:
1099 982 514
BsrDI GCAATG_nn'
Cuts at: 0 888 1929 1955 2589 2595
Size: 888 1041 26 634 6
Fragments arranged by size:
1041 888 634 26 6
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1860 2595
Size: 1860 735
BstYI r'GATC_y
Cuts at: 0 523 904 1581 2231 2595
Size: 523 381 677 650 364
Fragments arranged by size:
677 650 523 381 364
BstZ17I GTA'TAC
Cuts at: 0 87 2595
Size: 87 2508
BtgI C'CryG_G
Cuts at: 0 1871 2595
Size: 1871 724
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 360 2595
Size: 360 2235
BtsI GCAGTG_nn'
Cuts at: 0 1039 1611 2595
Size: 1039 572 984
Fragments arranged by size:
1039 984 572
ClaI AT'CG_AT
Cuts at: 0 258 2108 2595
Size: 258 1850 487
Fragments arranged by size:
1850 487 258
DraIII CAC_nnn'GTG
Cuts at: 0 1884 2595
Size: 1884 711
EaeI y'GGCC_r
Cuts at: 0 665 2595
Size: 665 1930
EarI CTCTTCn'nnn_
Cuts at: 0 138 1977 2243 2595
Size: 138 1839 266 352
Fragments arranged by size:
1839 352 266 138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1801 2595
Size: 1801 794
EcoICRI GAG'CTC
Cuts at: 0 788 2443 2595
Size: 788 1655 152
Fragments arranged by size:
1655 788 152
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 150 977 1552 2350 2595
Size: 18 132 827 575 798 245
Fragments arranged by size:
827 798 575 245 132 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2073 2595
Size: 2073 522
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1753 2595
Size: 1753 842
HaeII r_GCGC'y
Cuts at: 0 23 2595
Size: 23 2572
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 161 193 351 383 515 547 810 842
Size: 161 32 158 32 132 32 263 32
Cuts at: 842 1190 1222 1796 1828 2263 2295 2502 2532
Size: 348 32 574 32 435 32 207 30
Cuts at: 2532 2534 2548 2564 2580 2595
Size: 2 14 16 16 15
Fragments arranged by size:
574 435 348 263 207 161 158 132
32 32 32 32 32 32 32 30
16 16 15 14 2
HincII GTy'rAC
Cuts at: 0 511 1602 2595
Size: 511 1091 993
Fragments arranged by size:
1091 993 511
HpaI GTT'AAC
Cuts at: 0 1602 2595
Size: 1602 993
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 875 2237 2243 2259 2595
Size: 875 1362 6 16 336
Fragments arranged by size:
1362 875 336 16 6
MscI TGG'CCA
Cuts at: 0 667 2595
Size: 667 1928
MspA1I CmG'CkG
Cuts at: 0 188 1539 2034 2595
Size: 188 1351 495 561
Fragments arranged by size:
1351 561 495 188
NcoI C'CATG_G
Cuts at: 0 1871 2595
Size: 1871 724
NdeI CA'TA_TG
Cuts at: 0 66 2595
Size: 66 2529
NsiI A_TGCA'T
Cuts at: 0 217 2595
Size: 217 2378
NspI r_CATG'y
Cuts at: 0 219 2595
Size: 219 2376
PflMI CCAn_nnn'nTGG
Cuts at: 0 1121 2553 2595
Size: 1121 1432 42
Fragments arranged by size:
1432 1121 42
PfoI T'CCnGG_A
Cuts at: 0 944 1859 2595
Size: 944 915 736
Fragments arranged by size:
944 915 736
PstI C_TGCA'G
Cuts at: 0 1363 2595
Size: 1363 1232
PvuII CAG'CTG
Cuts at: 0 2034 2595
Size: 2034 561
SacI G_AGCT'C
Cuts at: 0 790 2445 2595
Size: 790 1655 150
Fragments arranged by size:
1655 790 150
SfcI C'TryA_G
Cuts at: 0 1359 2243 2595
Size: 1359 884 352
Fragments arranged by size:
1359 884 352
SmlI C'TyrA_G
Cuts at: 0 582 2595
Size: 582 2013
SphI G_CATG'C
Cuts at: 0 219 2595
Size: 219 2376
StuI AGG'CCT
Cuts at: 0 127 329 2595
Size: 127 202 2266
Fragments arranged by size:
2266 202 127
StyI C'CwwG_G
Cuts at: 0 324 1871 2348 2595
Size: 324 1547 477 247
Fragments arranged by size:
1547 477 324 247
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2374 2392 2515 2595
Size: 2374 18 123 80
Fragments arranged by size:
2374 123 80 18
Tth111I GACn'n_nGTC
Cuts at: 0 2526 2595
Size: 2526 69
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 952 2595
Size: 952 1643
XmnI GAAnn'nnTTC
Cuts at: 0 1348 2002 2595
Size: 1348 654 593
Fragments arranged by size:
1348 654 593
Enzymes that do cut:
AarI AccI AclI AcuI AhdI ApoI BamHI BanI
BanII BbsI BbvCI BclI BfrBI BglII BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoNI FalI HaeII Hin4I HincII
HpaI MmeI MscI MspA1I NcoI NdeI NsiI NspI
PflMI PfoI PstI PvuII SacI SfcI SmlI SphI
StuI StyI TaqII Tth111I XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AflIII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BcgI BciVI BglI BlpI Bme1580I
BmtI BplI BsaBI BsaWI BsgI BsiEI BsiWI BsmI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII Bsu36I CspCI DraI DrdI EagI EcoO109I
EcoRI EcoRV FseI FspI FspAI HindIII KasI KpnI
MfeI MluI MslI NaeI NarI NgoMIV NheI NotI
NruI PacI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
TatI TstI XbaI XhoI XmaI ZraI