################################## # NO CUT ENZYMES ( 177 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MluI MroI MseI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AciI 162 CCGC CCGC(-3/-1) AlwNI 50 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG AvaI 62 CCCGGG C^[CT]CG[GA]G BalI 176 TGGCCA TGG^CCA BpmI 82 CTGGAG CTGGAG(16/14) BsgI 165 CTGCAC GTGCAG(16/14) BsiYI 33 CCTCTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 33 CCTCTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 263 GAGAC GTCTC(1/5) BsmFI 233 GTCCC GGGAC(10/14) BsmI 138 GCATTC GAATGC(1/-1) BsoBI 62 CCCGGG C^[CT]CG[GA]G BsrI 114 CCAGT ACTGG(1/-1) BstAPI 125 GCAATTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 170 CATCC GGATG(2/0) BstXI 312 CCATGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 8 AGATCC [GA]^GATC[CT] CfoI 148 GCGC GCG^C DsaI 173 CCATGG C^C[GA][CT]GG EaeI 176 TGGCCA [CT]^GGCC[GA] EcoT14I 173 CCATGG C^C[AT][AT]GG FauI 161 CCCGC CCCGC(4/6) FokI 170 CATCC GGATG(9/13) HapII 63 CCGG C^CGG HhaI 148 GCGC GCG^C HinP1I 148 GCGC G^CGC HpaII 63 CCGG C^CGG MboII 6 GAAGA GAAGA(8/7) MfeI 126 CAATTG C^AATTG MflI 8 AGATCC [GA]^GATC[CT] MluNI 176 TGGCCA TGG^CCA MscI 176 TGGCCA TGG^CCA MspA1I 162 CCGCTG C[AC]G^C[GT]G MspI 63 CCGG C^CGG MunI 126 CAATTG C^AATTG NcoI 173 CCATGG C^CATGG SacI 94 GAGCTC GAGCT^C SfaNI 305 GCATC GCATC(5/9) SmaI 62 CCCGGG CCC^GGG StyI 173 CCATGG C^C[AT][AT]GG Tsp509I 127 AATT ^AATT TspRI 115 CAGTG CA[GC]TG[ACGT][ACGT]^ XhoII 8 AGATCC [GA]^GATC[CT] XmaCI 62 CCCGGG C^CCGGG XmaI 62 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AluI 51 AGCT AG^CT 95 AGCT AspHI 94 GAGCTC G[AT]GC[AT]^C 195 GTGCTC BanII 94 GAGCTC G[GA]GC[CT]^C 279 GGGCTC BcnI 62 CCCGG CC^[GC]GG 63 CCGGG BsaBI 9 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 69 GATCATTATC BsiHKAI 94 GAGCTC G[AT]GC[AT]^C 195 GTGCTC HphI 91 GGTGA GGTGA(8/7) 287 TCACC MamI 9 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 69 GATCATTATC MslI 139 CATTCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 168 CACATCCATG NciI 62 CCCGG CC^[GC]GG 63 CCGGG NlaIV 58 GGCCCC GG[ACGT]^[ACGT]CC 280 GGCTCC ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AlwI BbvI BmyI BsaJI Bsp1286I BssKI BstNI Cac8I Cfr13I DpnII EcoRII Fnu4HI HaeIII HgaI ItaI MboI MnlI MvaI MwoI NdeII NlaIII Sau3AI Sau96I ScrFI TseI