Restriction map for
pFN21AE6533
(Circular) (Six-base) MAPSORT of: pFN21AE6533.seq Check: 2059 from: 1 to: 5041
pFN21AE6533 5041 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2851 278
Size: 53 83 186 936 1315 2468
Fragments arranged by size:
2468 1315 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2384 1937
Size: 447 4594
Acc65I G'GTAC_C
Cuts at: 2362 2362
Size: 5041
AclI AA'CG_TT
Cuts at: 1563 3168 3541 1563
Size: 1605 373 3063
Fragments arranged by size:
3063 1605 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3104 4488 4756 1632
Size: 1472 1384 268 1917
Fragments arranged by size:
1917 1472 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4844
AflIII A'CryG_T
Cuts at: 1203 3940 4917 1203
Size: 2737 977 1327
Fragments arranged by size:
2737 1327 977
AgeI A'CCGG_T
Cuts at: 3872 3872
Size: 5041
AhdI GACnn_n'nnGTC
Cuts at: 3770 3770
Size: 5041
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5041
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2166 1184
Size: 982 4059
AlwNI CAG_nnn'CTG
Cuts at: 2060 2784 4356 2060
Size: 724 1572 2745
Fragments arranged by size:
2745 1572 724
ApaLI G'TGCA_C
Cuts at: 3098 4254 3098
Size: 1156 3885
ApoI r'AATT_y
Cuts at: 1493 2350 2576 2728 4837 1493
Size: 857 226 152 2109 1697
Fragments arranged by size:
2109 1697 857 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3595 160
Size: 872 2563 1606
Fragments arranged by size:
2563 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5041
AvaI C'yCGr_G
Cuts at: 1870 1943 2067 2366 1870
Size: 73 124 299 4545
Fragments arranged by size:
4545 299 124 73
BamHI G'GATC_C
Cuts at: 2371 2738 2371
Size: 367 4674
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2362 3817 618
Size: 325 217 627 575 1455 1842
Fragments arranged by size:
1842 1455 627 575 325 217
BanII G_rGCy'C
Cuts at: 1391 2103 2288 2360 1391
Size: 712 185 72 4072
Fragments arranged by size:
4072 712 185 72
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4070
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3232 3266 1903
Size: 34 1295 34 3678
Fragments arranged by size:
3678 1295 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2933 4143 2933
Size: 1210 3831
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5041
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3652 136
Size: 107 122 71 631 605 1980 1525
Fragments arranged by size:
1980 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5036 5036
Size: 5041
BlpI GC'TnA_GC
Cuts at: 2458 2458
Size: 5041
Bme1580I G_kGCm'C
Cuts at: 1790 2119 3102 4258 1790
Size: 329 983 1156 2573
Fragments arranged by size:
2573 1156 983 329
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5041
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2113 2772 3730
Size: 416 53 83 399 24 696 659 958
Cuts at: 3730 442
Size: 1753
Fragments arranged by size:
1753 958 696 659 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5041
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2108 3701 1239
Size: 869 1593 2579
Fragments arranged by size:
2579 1593 869
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3166 4031 4329 3166
Size: 865 298 3878
Fragments arranged by size:
3878 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3704 882
Size: 617 2205 2219
Fragments arranged by size:
2219 2205 617
BsaAI yAC'GTr
Cuts at: 493 1204 2832 493
Size: 711 1628 2702
Fragments arranged by size:
2702 1628 711
BsaBI GATnn'nnATC
Cuts at: 2018 2078 5035 2018
Size: 60 2957 2024
Fragments arranged by size:
2957 2024 60
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2848 3230 3865 275
Size: 53 83 186 936 1315 382 635 1451
Fragments arranged by size:
1451 1315 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3474 3872 4146 4293 4704 3474
Size: 398 274 147 411 3811
Fragments arranged by size:
3811 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4816
Fragments arranged by size:
4816 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5041
BseYI C'CCAG_C
Cuts at: 2155 4244 4740 2155
Size: 2089 496 2456
Fragments arranged by size:
2456 2089 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2155 1163
Size: 573 419 4049
Fragments arranged by size:
4049 573 419
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2491 3252 4280 1540
Size: 275 186 490 761 1028 2301
Fragments arranged by size:
2301 1028 761 490 275 186
BsiHKAI G_wGCw'C
Cuts at: 2103 2204 3102 3187 4258 2103
Size: 101 898 85 1071 2886
Fragments arranged by size:
2886 1071 898 101 85
BsmI GAATG_Cn'
Cuts at: 2143 2552 2645 2143
Size: 409 93 4539
Fragments arranged by size:
4539 409 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 2119 2204 2288 2360 3102 3187
Size: 399 313 16 85 84 72 742 85
Cuts at: 3187 4258 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 313 85 85 84
72 16
BspEI T'CCGG_A
Cuts at: 4704 4704
Size: 5041
BspHI T'CATG_A
Cuts at: 2930 4660 2930
Size: 1730 3311
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2329 2396 844
Size: 1485 67 3489
Fragments arranged by size:
3489 1485 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2928 1394
Size: 429 1105 3507
Fragments arranged by size:
3507 1105 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3536 3710 65
Size: 1183 2288 174 1396
Fragments arranged by size:
2288 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3685 3872 1700
Size: 110 1875 187 2869
Fragments arranged by size:
2869 1875 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5041
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5041
BssSI C'ACGA_G
Cuts at: 3101 4113 3101
Size: 1012 4029
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2136 794
Size: 1342 3699
BstBI TT'CG_AA
Cuts at: 3845 3845
Size: 5041
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5041
BstXI CCAn_nnnn'nTGG
Cuts at: 2324 2324
Size: 5041
BstYI r'GATC_y
Cuts at: 1972 2013 2371 2738 3123 3140 4592 4678 4690
Size: 41 358 367 385 17 1452 86 12
Cuts at: 4690 5036 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 358 346 86 41
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2178 513
Size: 552 939 174 3376
Fragments arranged by size:
3376 939 552 174
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4302
Fragments arranged by size:
4302 683 56
BtsI GCAGTG_nn'
Cuts at: 2557 3351 3371 2557
Size: 794 20 4227
Fragments arranged by size:
4227 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5041
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5006
DraI TTT'AAA
Cuts at: 2345 2345
Size: 5041
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4048 809
Size: 507 2732 1802
Fragments arranged by size:
2732 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2181 2488 3377
Size: 54 1425 50 275 18 351 307 889
Cuts at: 3377 5006 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 351 307 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2488 1537
Size: 275 676 4090
Fragments arranged by size:
4090 676 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2971 1353
Size: 1618 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3628 4002 4148 387
Size: 1064 138 2039 374 146 1280
Fragments arranged by size:
2039 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2101 2101
Size: 5041
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 3104 3701 4488 4756 1239
Size: 393 476 996 597 787 268 1524
Fragments arranged by size:
1524 996 787 597 476 393 268
EcoRI G'AATT_C
Cuts at: 2350 2728 2350
Size: 378 4663
FspI TGC'GCA
Cuts at: 3547 3547
Size: 5041
HaeII r_GCGC'y
Cuts at: 1678 4188 1678
Size: 2510 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3682 3714 3756 3788 1373
Size: 32 135 32 2110 32 42 32 2626
Fragments arranged by size:
2626 2110 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2385 2631 669
Size: 988 281 447 246 3079
Fragments arranged by size:
3079 988 447 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5041
HpaI GTT'AAC
Cuts at: 2631 2631
Size: 5041
KpnI G_GTAC'C
Cuts at: 2366 2366
Size: 5041
MfeI C'AATT_G
Cuts at: 2131 2640 2131
Size: 509 4532
MluI A'CGCG_T
Cuts at: 4917 4917
Size: 5041
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4155 4339 1305
Size: 2850 184 2007
Fragments arranged by size:
2850 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2183 10
Size: 54 1425 343 351 2868
Fragments arranged by size:
2868 1425 351 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2148 2177 2999 3358 3517 518
Size: 648 982 29 822 359 159 2042
Fragments arranged by size:
2042 982 822 648 359 159 29
MspA1I CmG'CkG
Cuts at: 1606 2169 2457 3134 4282 4527 1606
Size: 563 288 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 563 288 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2178 513
Size: 552 939 174 3376
Fragments arranged by size:
3376 939 552 174
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5041
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5041
NotI GC'GGCC_GC
Cuts at: 2488 2488
Size: 5041
NspI r_CATG'y
Cuts at: 1132 1229 2399 3944 1132
Size: 97 1170 1545 2229
Fragments arranged by size:
2229 1545 1170 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5041
PciI A'CATG_T
Cuts at: 3940 3940
Size: 5041
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5041
PmeI GTTT'AAAC
Cuts at: 2345 2345
Size: 5041
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3110 3142 4623 4655 3110
Size: 32 1481 32 3496
Fragments arranged by size:
3496 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5041
PsiI TTA'TAA
Cuts at: 2611 2611
Size: 5041
PstI C_TGCA'G
Cuts at: 830 2393 830
Size: 1563 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5041
SacI G_AGCT'C
Cuts at: 2103 2103
Size: 5041
SalI G'TCGA_C
Cuts at: 1936 2383 1936
Size: 447 4594
SbfI CC_TGCA'GG
Cuts at: 2393 2393
Size: 5041
ScaI AGT'ACT
Cuts at: 3289 5001 3289
Size: 1712 3329
SfcI C'TryA_G
Cuts at: 826 1045 2389 3524 4205 4396 826
Size: 219 1344 1135 681 191 1471
Fragments arranged by size:
1471 1344 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5041
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5041
SmaI CCC'GGG
Cuts at: 1872 2069 2368 1872
Size: 197 299 4545
Fragments arranged by size:
4545 299 197
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3145 4046 4308 820
Size: 197 926 1202 901 262 1553
Fragments arranged by size:
1553 1202 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2832 493
Size: 2339 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5041
SphI G_CATG'C
Cuts at: 1132 2399 1132
Size: 1267 3774
SspI AAT'ATT
Cuts at: 5 52 2965 5
Size: 47 2913 2081
Fragments arranged by size:
2913 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5041
StyI C'CwwG_G
Cuts at: 513 1065 2004 2178 2480 513
Size: 552 939 174 302 3074
Fragments arranged by size:
3074 939 552 302 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3068 3085 3238 3423 3868 325
Size: 1255 1488 17 153 185 445 1498
Fragments arranged by size:
1498 1488 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3287 4999 96
Size: 274 80 33 51 1443 1310 1712 138
Fragments arranged by size:
1712 1443 1310 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5001 5033 5001
Size: 32 5009
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5041
XbaI T'CTAG_A
Cuts at: 2377 2377
Size: 5041
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5041
XmaI C'CCGG_G
Cuts at: 1870 2067 2366 1870
Size: 197 299 4545
Fragments arranged by size:
4545 299 197
XmnI GAAnn'nnTTC
Cuts at: 3170 3170
Size: 5041
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2849 276
Size: 53 83 186 936 1315 2468
Fragments arranged by size:
2468 1315 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI