################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AatII AccI AclI AflII AflIII AgeI AlwNI AocI Aor51HI AscI AseI AsnI Asp700I AspI AviII AvrII BbrPI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsmBI BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI MfeI MluI MunI NdeI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI RsrII SalI SapI SbfI SexAI SfiI SfuI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 310 GGTACC G^GTACC AccIII 926 TCCGGA T^CCGGA AcsI 709 AAATTT [GA]^AATT[CT] AhdI 237 GACCGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1015 GTGCAC G^TGCAC ApaLI 1015 GTGCAC G^TGCAC ApoI 709 AAATTT [GA]^AATT[CT] Asp718I 310 GGTACC G^GTACC AspEI 237 GACCGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 113 TGGCCA TGG^CCA BamHI 408 GGATCC G^GATCC BbsI 262 GAAGAC GAAGAC(2/6) BbvCI 510 GCTGAGG CCTCAGC(-5/-2) BcgI 615 CGACTTGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 262 GAAGAC GAAGAC(2/6) BsaBI 591 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 926 TCCGGA [AT]^CCGG[AT] BseAI 926 TCCGGA T^CCGGA BsgI 703 GTGCAG GTGCAG(16/14) BsiWI 240 CGTACG C^GTACG BsmI 813 GAATGC GAATGC(1/-1) Bsp1407I 524 TGTACA T^GTACA BspEI 926 TCCGGA T^CCGGA BsrGI 524 TGTACA T^GTACA BstF5I 621 GGATG GGATG(2/0) BstYI 408 GGATCC [GA]^GATC[CT] EagI 183 CGGCCG C^GGCCG Eam1105I 237 GACCGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 183 CGGCCG C^GGCCG Eco52I 183 CGGCCG C^GGCCG FokI 621 GGATG GGATG(9/13) HincII 100 GTTGAC GT[CT]^[GA]AC HindII 100 GTTGAC GT[CT]^[GA]AC KpnI 310 GGTACC GGTAC^C KspI 180 CCGCGG CCGC^GG MaeIII 245 GTCAC ^GT[ACGT]AC MamI 591 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 408 GGATCC [GA]^GATC[CT] MluNI 113 TGGCCA TGG^CCA MroI 926 TCCGGA T^CCGGA MscI 113 TGGCCA TGG^CCA MslI 105 CACCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 627 CCATGG C^CATGG NheI 855 GCTAGC G^CTAGC PpuMI 740 GGGACCT [GA]G^G[AT]CC[CT] PshAI 657 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 961 GAGCTC GAGCT^C SacII 180 CCGCGG CCGC^GG SfcI 651 CTACAG C^T[GA][CT]AG SgrAI 117 CACCGGCG C[GA]^CCGG[CT]G SmlI 667 CTCAAG C^T[CT][GA]AG SspBI 524 TGTACA T^GTACA Tsp45I 245 GTCAC ^GT[GC]AC XhoII 408 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### ApaI 194 GGGCCC GGGCC^C 1031 GGGCCC AspHI 961 GAGCTC G[AT]GC[AT]^C 1015 GTGCAC BsaI 235 GAGACC GGTCTC(1/5) 361 GAGACC BseRI 1072 CTCCTC GAGGAG(10/8) 513 GAGGAG BsiHKAI 961 GAGCTC G[AT]GC[AT]^C 1015 GTGCAC BssHII 436 GCGCGC G^CGCGC 438 GCGCGC EcoNI 202 CCTGGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 480 CCTGTTTGAGG EcoT14I 163 CCTTGG C^C[AT][AT]GG 627 CCATGG HgaI 225 GACGC GACGC(5/10) 490 GACGC MaeII 110 ACGT A^CGT 243 ACGT MseI 374 TTAA T^TAA 896 TTAA NaeI 185 GCCGGC GCC^GGC 971 GCCGGC NgoMIV 185 GCCGGC G^CCGGC 971 GCCGGC PflMI 730 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1035 CCAGCTAGTGG ScaI 723 AGTACT AGT^ACT 887 AGTACT StuI 477 AGGCCT AGG^CCT 671 AGGCCT StyI 163 CCTTGG C^C[AT][AT]GG 627 CCATGG TaqI 23 TCGA T^CGA 614 TCGA TfiI 596 GAATC G^A[AT]TC 878 GATTC Tru9I 374 TTAA T^TAA 896 TTAA Tsp509I 633 AATT ^AATT 710 AATT TthHB8I 23 TCGA T^CGA 614 TCGA Van91I 730 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1035 CCAGCTAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AvaI AvaII BanI BanII BbeI BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BsiEI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfoI SmaI TseI TspRI XmaCI XmaI