(Circular) (Six-base) MAPSORT of: pFN21AE3152.seq Check: 8322 from: 1 to: 5797 pFN21AE3152 5797 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3607 278 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3140 1937 Size: 1203 4594 Acc65I G'GTAC_C Cuts at: 2315 3118 2315 Size: 803 4994 AclI AA'CG_TT Cuts at: 1563 3924 4297 1563 Size: 2361 373 3063 Fragments arranged by size: 3063 2361 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2591 2709 2773 3051 3860 5244 5512 1632 Size: 959 118 64 278 809 1384 268 1917 Fragments arranged by size: 1917 1384 959 809 278 268 118 64 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5600 AflIII A'CryG_T Cuts at: 1203 4696 5673 1203 Size: 3493 977 1327 Fragments arranged by size: 3493 1327 977 AgeI A'CCGG_T Cuts at: 4628 4628 Size: 5797 AhdI GACnn_n'nnGTC Cuts at: 2247 4526 2247 Size: 2279 3518 AleI CACnn'nnGTG Cuts at: 1166 2114 1166 Size: 948 4849 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5797 AlwNI CAG_nnn'CTG Cuts at: 3540 5112 3540 Size: 1572 4225 ApaI G_GGCC'C Cuts at: 2203 3040 2203 Size: 837 4960 ApaLI G'TGCA_C Cuts at: 3020 3854 5010 3020 Size: 834 1156 3807 Fragments arranged by size: 3807 1156 834 ApoI r'AATT_y Cuts at: 1493 2714 3106 3332 3484 5593 1493 Size: 1221 392 226 152 2109 1697 Fragments arranged by size: 2109 1697 1221 392 226 152 AseI AT'TA_AT Cuts at: 160 1032 4351 160 Size: 872 3319 1606 Fragments arranged by size: 3319 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 AvaI C'yCGr_G Cuts at: 1870 1943 2070 2153 2234 2328 3122 1870 Size: 73 127 83 81 94 794 4545 Fragments arranged by size: 4545 794 127 94 83 81 73 BamHI G'GATC_C Cuts at: 2413 3127 3494 2413 Size: 714 367 4716 Fragments arranged by size: 4716 714 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2126 2315 2907 2983 3118 Size: 325 217 627 339 189 592 76 135 Cuts at: 3118 4573 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 592 339 325 217 189 135 76 BanII G_rGCy'C Cuts at: 1391 2203 2970 3040 3116 1391 Size: 812 767 70 76 4072 Fragments arranged by size: 4072 812 767 76 70 BbeI G_GCGC'C Cuts at: 2130 2911 2987 2130 Size: 781 76 4940 Fragments arranged by size: 4940 781 76 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2274 928 Size: 971 375 4451 Fragments arranged by size: 4451 971 375 BbvCI CC'TCA_GC Cuts at: 2516 2516 Size: 5797 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2609 2643 3988 4022 1903 Size: 34 50 34 588 34 1345 34 3678 Fragments arranged by size: 3678 1345 588 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3689 4899 3689 Size: 1210 4587 BclI T'GATC_A Cuts at: 1548 1548 Size: 5797 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4408 136 Size: 107 122 71 631 605 2736 1525 Fragments arranged by size: 2736 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5792 5792 Size: 5797 BlpI GC'TnA_GC Cuts at: 3214 3214 Size: 5797 Bme1580I G_kGCm'C Cuts at: 1790 2168 2203 3024 3040 3858 5014 1790 Size: 378 35 821 16 818 1156 2573 Fragments arranged by size: 2573 1156 821 818 378 35 16 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5797 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3528 4486 442 Size: 416 53 83 399 24 2111 958 1753 Fragments arranged by size: 2111 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2864 1056 Size: 1808 3989 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2244 2844 2892 4457 1239 Size: 1005 600 48 1565 2579 Fragments arranged by size: 2579 1565 1005 600 48 Bpu10I CC'TnA_GC Cuts at: 2516 2921 2516 Size: 405 5392 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2657 3922 4787 5085 2657 Size: 1265 865 298 3369 Fragments arranged by size: 3369 1265 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2234 2360 4460 882 Size: 617 735 126 2100 2219 Fragments arranged by size: 2219 2100 735 617 126 BsaAI yAC'GTr Cuts at: 493 1204 3588 493 Size: 711 2384 2702 Fragments arranged by size: 2702 2384 711 BsaBI GATnn'nnATC Cuts at: 2600 5791 2600 Size: 3191 2606 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2127 2231 2908 2984 Size: 53 83 186 936 594 104 677 76 Cuts at: 2984 3604 3986 4621 275 Size: 620 382 635 1451 Fragments arranged by size: 1451 936 677 635 620 594 382 186 104 83 76 53 BsaWI w'CCGG_w Cuts at: 2931 4230 4628 4902 5049 5460 2931 Size: 1299 398 274 147 411 3268 Fragments arranged by size: 3268 1299 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5572 Fragments arranged by size: 5572 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2533 3068 1186 Size: 1347 535 3915 Fragments arranged by size: 3915 1347 535 BseYI C'CCAG_C Cuts at: 2611 2699 2846 2876 3039 5000 5496 2611 Size: 88 147 30 163 1961 496 2912 Fragments arranged by size: 2912 1961 496 163 147 88 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2729 1163 Size: 573 993 4231 Fragments arranged by size: 4231 993 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2191 2563 2693 3247 4008 5036 Size: 275 186 190 372 130 554 761 1028 Cuts at: 5036 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 554 372 275 190 186 130 BsiHKAI G_wGCw'C Cuts at: 2970 3024 3858 3943 5014 2970 Size: 54 834 85 1071 3753 Fragments arranged by size: 3753 1071 834 85 54 BsiWI C'GTAC_G Cuts at: 2245 2245 Size: 5797 BsmI GAATG_Cn' Cuts at: 2824 3308 3401 2824 Size: 484 93 5220 Fragments arranged by size: 5220 484 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2168 2203 2970 3024 3040 3116 3858 Size: 399 378 35 767 54 16 76 742 Cuts at: 3858 3943 5014 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 767 742 399 378 85 76 54 35 16 BspEI T'CCGG_A Cuts at: 2931 5460 2931 Size: 2529 3268 BspHI T'CATG_A Cuts at: 3686 5416 3686 Size: 1730 4067 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3152 844 Size: 2308 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3684 1394 Size: 429 1861 3507 Fragments arranged by size: 3507 1861 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4292 4466 65 Size: 1183 3044 174 1396 Fragments arranged by size: 3044 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2123 2190 2562 2976 4441 4628 1700 Size: 110 313 67 372 414 1465 187 2869 Fragments arranged by size: 2869 1465 414 372 313 187 110 67 BsrGI T'GTAC_A Cuts at: 96 2529 96 Size: 2433 3364 BssHII G'CGCG_C Cuts at: 1925 2441 2443 1925 Size: 516 2 5279 Fragments arranged by size: 5279 516 2 BssSI C'ACGA_G Cuts at: 3857 4869 3857 Size: 1012 4785 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5797 BstBI TT'CG_AA Cuts at: 4601 4601 Size: 5797 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5797 BstYI r'GATC_y Cuts at: 1972 2413 3127 3494 3879 3896 5348 5434 5446 Size: 441 714 367 385 17 1452 86 12 Cuts at: 5446 5792 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 714 441 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2185 2553 2632 513 Size: 552 1120 368 79 3678 Fragments arranged by size: 3678 1120 552 368 79 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5058 Fragments arranged by size: 5058 683 56 BtsI GCAGTG_nn' Cuts at: 2251 2712 3313 4107 4127 2251 Size: 461 601 794 20 3921 Fragments arranged by size: 3921 794 601 461 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5797 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5762 DraI TTT'AAA Cuts at: 3101 3101 Size: 5797 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4804 809 Size: 507 3488 1802 Fragments arranged by size: 3488 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2118 2188 2556 Size: 54 1425 50 275 18 288 70 368 Cuts at: 2556 2686 3244 4133 5762 8 Size: 130 558 889 1629 43 Fragments arranged by size: 1629 1425 889 558 368 288 275 130 70 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2188 3244 1537 Size: 275 376 1056 4090 Fragments arranged by size: 4090 1056 376 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2293 2339 2424 3727 1353 Size: 940 46 85 1303 3423 Fragments arranged by size: 3423 1303 940 85 46 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2226 2404 3071 4384 4758 4904 Size: 1064 138 637 178 667 1313 374 146 Cuts at: 4904 387 Size: 1280 Fragments arranged by size: 1313 1280 1064 667 637 374 178 146 138 EcoICRI GAG'CTC Cuts at: 2968 2968 Size: 5797 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2244 2591 2709 2773 2844 2892 3051 Size: 393 612 347 118 64 71 48 159 Cuts at: 3051 3860 4457 5244 5512 1239 Size: 809 597 787 268 1524 Fragments arranged by size: 1524 809 787 612 597 393 347 268 159 118 71 64 48 EcoNI CCTnn'n_nnAGG Cuts at: 2211 2489 2211 Size: 278 5519 EcoO109I rG'GnC_Cy Cuts at: 2199 2399 2746 3036 2199 Size: 200 347 290 4960 Fragments arranged by size: 4960 347 290 200 EcoRI G'AATT_C Cuts at: 3106 3484 3106 Size: 378 5419 FspI TGC'GCA Cuts at: 4303 4303 Size: 5797 HaeII r_GCGC'y Cuts at: 1678 2130 2911 2987 4944 1678 Size: 452 781 76 1957 2531 Fragments arranged by size: 2531 1957 781 452 76 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2233 2265 2420 2452 3041 Size: 32 135 32 661 32 155 32 589 Cuts at: 3041 3073 4438 4470 4512 4544 1373 Size: 32 1365 32 42 32 2626 Fragments arranged by size: 2626 1365 661 589 155 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2107 3141 3387 669 Size: 988 281 169 1034 246 3079 Fragments arranged by size: 3079 1034 988 281 246 169 HindIII A'AGCT_T Cuts at: 748 748 Size: 5797 HpaI GTT'AAC Cuts at: 3387 3387 Size: 5797 KasI G'GCGC_C Cuts at: 2126 2907 2983 2126 Size: 781 76 4940 Fragments arranged by size: 4940 781 76 KpnI G_GTAC'C Cuts at: 2319 3122 2319 Size: 803 4994 MfeI C'AATT_G Cuts at: 3396 3396 Size: 5797 MluI A'CGCG_T Cuts at: 5673 5673 Size: 5797 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2391 4911 5095 1305 Size: 1086 2520 184 2007 Fragments arranged by size: 2520 2007 1086 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2120 10 Size: 54 1425 343 288 3687 Fragments arranged by size: 3687 1425 343 288 54 MslI CAynn'nnrTG Cuts at: 518 1166 2114 3755 4114 4273 518 Size: 648 948 1641 359 159 2042 Fragments arranged by size: 2042 1641 948 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2187 2470 2569 2611 3213 3890 5038 5283 Size: 581 283 99 42 602 677 1148 245 Cuts at: 5283 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 602 581 283 245 99 42 NaeI GCC'GGC Cuts at: 2192 2978 2192 Size: 786 5011 NarI GG'CG_CC Cuts at: 2127 2908 2984 2127 Size: 781 76 4940 Fragments arranged by size: 4940 781 76 NcoI C'CATG_G Cuts at: 513 1065 2632 513 Size: 552 1567 3678 Fragments arranged by size: 3678 1567 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5797 NgoMIV G'CCGG_C Cuts at: 2190 2976 2190 Size: 786 5011 NheI G'CTAG_C Cuts at: 1052 2860 1052 Size: 1808 3989 NotI GC'GGCC_GC Cuts at: 3244 3244 Size: 5797 NspI r_CATG'y Cuts at: 1132 1229 3155 4700 1132 Size: 97 1926 1545 2229 Fragments arranged by size: 2229 1926 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5797 PciI A'CATG_T Cuts at: 4696 4696 Size: 5797 PflMI CCAn_nnn'nTGG Cuts at: 1108 2741 3046 1108 Size: 1633 305 3859 Fragments arranged by size: 3859 1633 305 PfoI T'CCnGG_A Cuts at: 2361 2361 Size: 5797 PmeI GTTT'AAAC Cuts at: 3101 3101 Size: 5797 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3866 3898 5379 5411 3866 Size: 32 1481 32 4252 Fragments arranged by size: 4252 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2746 2746 Size: 5797 PshAI GACnn'nnGTC Cuts at: 1613 2666 1613 Size: 1053 4744 PsiI TTA'TAA Cuts at: 3367 3367 Size: 5797 PspOMI G'GGCC_C Cuts at: 2199 3036 2199 Size: 837 4960 PstI C_TGCA'G Cuts at: 830 3149 830 Size: 2319 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5797 PvuII CAG'CTG Cuts at: 2470 2569 2611 2470 Size: 99 42 5656 Fragments arranged by size: 5656 99 42 SacI G_AGCT'C Cuts at: 2970 2970 Size: 5797 SacII CC_GC'GG Cuts at: 2188 2188 Size: 5797 SalI G'TCGA_C Cuts at: 1936 3139 1936 Size: 1203 4594 SbfI CC_TGCA'GG Cuts at: 3149 3149 Size: 5797 ScaI AGT'ACT Cuts at: 2730 2894 4045 5757 2730 Size: 164 1151 1712 2770 Fragments arranged by size: 2770 1712 1151 164 SfcI C'TryA_G Cuts at: 826 1045 2656 3145 4280 4961 5152 826 Size: 219 1611 489 1135 681 191 1471 Fragments arranged by size: 1611 1471 1135 681 489 219 191 SfoI GGC'GCC Cuts at: 2128 2909 2985 2128 Size: 781 76 4940 Fragments arranged by size: 4940 781 76 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 SgrAI Cr'CCGG_yG Cuts at: 1700 2123 1700 Size: 423 5374 SmaI CCC'GGG Cuts at: 1872 2072 2155 2236 2330 3124 1872 Size: 200 83 81 94 794 4545 Fragments arranged by size: 4545 794 200 94 83 81 SmlI C'TyrA_G Cuts at: 820 1017 1943 2672 3901 4802 5064 820 Size: 197 926 729 1229 901 262 1553 Fragments arranged by size: 1553 1229 926 901 729 262 197 SnaBI TAC'GTA Cuts at: 493 3588 493 Size: 3095 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5797 SphI G_CATG'C Cuts at: 1132 3155 1132 Size: 2023 3774 SspI AAT'ATT Cuts at: 5 52 3721 5 Size: 47 3669 2081 Fragments arranged by size: 3669 2081 47 StuI AGG'CCT Cuts at: 1517 2484 2678 1517 Size: 967 194 4636 Fragments arranged by size: 4636 967 194 StyI C'CwwG_G Cuts at: 513 1065 2168 2632 3236 513 Size: 552 1103 464 604 3074 Fragments arranged by size: 3074 1103 604 552 464 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3824 3841 3994 4179 4624 325 Size: 1255 2244 17 153 185 445 1498 Fragments arranged by size: 2244 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2529 2728 2892 Size: 274 80 33 51 1443 552 199 164 Cuts at: 2892 4043 5755 96 Size: 1151 1712 138 Fragments arranged by size: 1712 1443 1151 552 274 199 164 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5757 5789 5757 Size: 32 5765 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5797 XbaI T'CTAG_A Cuts at: 3133 3133 Size: 5797 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5797 XmaI C'CCGG_G Cuts at: 1870 2070 2153 2234 2328 3122 1870 Size: 200 83 81 94 794 4545 Fragments arranged by size: 4545 794 200 94 83 81 XmnI GAAnn'nnTTC Cuts at: 3926 3926 Size: 5797 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3605 276 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI XcmI