(Circular) (Six-base) MAPSORT of: pFN21AE6663.seq Check: 1793 from: 1 to: 5560 pFN21AE6663 5560 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2526 2526 Size: 5560 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3370 278 Size: 53 83 186 936 1834 2468 Fragments arranged by size: 2468 1834 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2903 1937 Size: 966 4594 Acc65I G'GTAC_C Cuts at: 2776 2881 2776 Size: 105 5455 AclI AA'CG_TT Cuts at: 1563 3687 4060 1563 Size: 2124 373 3063 Fragments arranged by size: 3063 2124 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3623 5007 5275 1632 Size: 1991 1384 268 1917 Fragments arranged by size: 1991 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5363 AflIII A'CryG_T Cuts at: 1203 2466 4459 5436 1203 Size: 1263 1993 977 1327 Fragments arranged by size: 1993 1327 1263 977 AgeI A'CCGG_T Cuts at: 4391 4391 Size: 5560 AhdI GACnn_n'nnGTC Cuts at: 4289 4289 Size: 5560 AleI CACnn'nnGTG Cuts at: 1166 2240 2555 1166 Size: 1074 315 4171 Fragments arranged by size: 4171 1074 315 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2339 1184 Size: 1155 4405 AlwNI CAG_nnn'CTG Cuts at: 2657 3303 4875 2657 Size: 646 1572 3342 Fragments arranged by size: 3342 1572 646 ApaLI G'TGCA_C Cuts at: 3617 4773 3617 Size: 1156 4404 ApoI r'AATT_y Cuts at: 1493 2869 3095 3247 5356 1493 Size: 1376 226 152 2109 1697 Fragments arranged by size: 2109 1697 1376 226 152 AseI AT'TA_AT Cuts at: 160 1032 4114 160 Size: 872 3082 1606 Fragments arranged by size: 3082 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5560 AvaI C'yCGr_G Cuts at: 1870 1943 2503 2885 1870 Size: 73 560 382 4545 Fragments arranged by size: 4545 560 382 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2760 2768 2793 2801 2760 Size: 8 25 8 5519 Fragments arranged by size: 5519 25 8 8 BamHI G'GATC_C Cuts at: 2890 3257 2890 Size: 367 5193 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2776 2810 2881 4336 618 Size: 325 217 627 989 34 71 1455 1842 Fragments arranged by size: 1842 1455 989 627 325 217 71 34 BanII G_rGCy'C Cuts at: 1391 2285 2340 2820 2879 1391 Size: 894 55 480 59 4072 Fragments arranged by size: 4072 894 480 59 55 BbeI G_GCGC'C Cuts at: 2814 2814 Size: 5560 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4589 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3751 3785 1903 Size: 34 1814 34 3678 Fragments arranged by size: 3678 1814 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3452 4662 3452 Size: 1210 4350 BclI T'GATC_A Cuts at: 1548 1548 Size: 5560 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2585 4171 136 Size: 107 122 71 631 605 913 1586 1525 Fragments arranged by size: 1586 1525 913 631 605 122 107 71 BglII A'GATC_T Cuts at: 5555 5555 Size: 5560 BlpI GC'TnA_GC Cuts at: 2819 2840 2977 2819 Size: 21 137 5402 Fragments arranged by size: 5402 137 21 Bme1580I G_kGCm'C Cuts at: 1790 2697 3621 4777 1790 Size: 907 924 1156 2573 Fragments arranged by size: 2573 1156 924 907 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5560 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2180 3291 4249 Size: 416 53 83 399 24 763 1111 958 Cuts at: 4249 442 Size: 1753 Fragments arranged by size: 1753 1111 958 763 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5560 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2304 2304 Size: 5560 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2299 2819 4220 1239 Size: 1060 520 1401 2579 Fragments arranged by size: 2579 1401 1060 520 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3685 4550 4848 3685 Size: 865 298 4397 Fragments arranged by size: 4397 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2531 4223 882 Size: 617 1032 1692 2219 Fragments arranged by size: 2219 1692 1032 617 BsaAI yAC'GTr Cuts at: 493 1204 3351 493 Size: 711 2147 2702 Fragments arranged by size: 2702 2147 711 BsaBI GATnn'nnATC Cuts at: 5554 5554 Size: 5560 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 2075 2811 3367 Size: 53 83 186 936 479 63 736 556 Cuts at: 3367 3749 4384 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 736 635 556 479 382 186 83 63 53 BsaWI w'CCGG_w Cuts at: 3993 4391 4665 4812 5223 3993 Size: 398 274 147 411 4330 Fragments arranged by size: 4330 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5335 Fragments arranged by size: 5335 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2216 2460 1186 Size: 1030 244 4286 Fragments arranged by size: 4286 1030 244 BseYI C'CCAG_C Cuts at: 2026 2146 2215 4763 5259 2026 Size: 120 69 2548 496 2327 Fragments arranged by size: 2548 2327 496 120 69 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2693 1163 Size: 573 957 4030 Fragments arranged by size: 4030 957 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2241 2404 3010 3771 4799 1540 Size: 275 186 240 163 606 761 1028 2301 Fragments arranged by size: 2301 1028 761 606 275 240 186 163 BsiHKAI G_wGCw'C Cuts at: 2139 2841 3621 3706 4777 2139 Size: 702 780 85 1071 2922 Fragments arranged by size: 2922 1071 780 702 85 BsmI GAATG_Cn' Cuts at: 3071 3164 3071 Size: 93 5467 BsmBI CGTCTCn'nnnn_ Cuts at: 2352 2491 2352 Size: 139 5421 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2139 2285 2340 2697 2820 2841 2879 Size: 399 349 146 55 357 123 21 38 Cuts at: 2879 3621 3706 4777 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 357 349 146 123 85 55 38 21 BspEI T'CCGG_A Cuts at: 5223 5223 Size: 5560 BspHI T'CATG_A Cuts at: 3449 5179 3449 Size: 1730 3830 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2526 2915 844 Size: 1682 389 3489 Fragments arranged by size: 3489 1682 389 BsrBI CCG'CTC Cuts at: 1394 1823 2108 2288 3447 1394 Size: 429 285 180 1159 3507 Fragments arranged by size: 3507 1159 429 285 180 BsrDI GCAATG_nn' Cuts at: 65 1248 2207 2604 4055 4229 65 Size: 1183 959 397 1451 174 1396 Fragments arranged by size: 1451 1396 1183 959 397 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2399 4204 4391 1700 Size: 110 589 1805 187 2869 Fragments arranged by size: 2869 1805 589 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5560 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5560 BssSI C'ACGA_G Cuts at: 3620 4632 3620 Size: 1012 4548 BstAPI GCAn_nnn'nTGC Cuts at: 794 2143 794 Size: 1349 4211 BstBI TT'CG_AA Cuts at: 4364 4364 Size: 5560 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5560 BstYI r'GATC_y Cuts at: 1972 2113 2725 2890 3257 3642 3659 5111 5197 Size: 141 612 165 367 385 17 1452 86 Cuts at: 5197 5209 5555 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 612 385 367 346 165 141 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2199 2199 Size: 5560 BtgI C'CryG_G Cuts at: 513 1065 2004 2713 513 Size: 552 939 709 3360 Fragments arranged by size: 3360 939 709 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4821 Fragments arranged by size: 4821 683 56 BtsI GCAGTG_nn' Cuts at: 2610 2747 3076 3870 3890 2610 Size: 137 329 794 20 4280 Fragments arranged by size: 4280 794 329 137 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5560 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5525 DraI TTT'AAA Cuts at: 2864 2864 Size: 5560 DraIII CAC_nnn'GTG Cuts at: 2143 2143 Size: 5560 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2483 4567 809 Size: 507 1167 2084 1802 Fragments arranged by size: 2084 1802 1167 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2118 2238 2401 Size: 54 1425 50 275 18 288 120 163 Cuts at: 2401 3007 3896 5525 8 Size: 606 889 1629 43 Fragments arranged by size: 1629 1425 889 606 288 275 163 120 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2238 2401 3007 1537 Size: 275 426 163 606 4090 Fragments arranged by size: 4090 606 426 275 163 EarI CTCTTCn'nnn_ Cuts at: 1353 2399 3490 1353 Size: 1046 1091 3423 Fragments arranged by size: 3423 1091 1046 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2477 4147 4521 4667 387 Size: 1064 138 888 1670 374 146 1280 Fragments arranged by size: 1670 1280 1064 888 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2299 2819 3623 4220 5007 5275 1239 Size: 393 667 520 804 597 787 268 1524 Fragments arranged by size: 1524 804 787 667 597 520 393 268 EcoO109I rG'GnC_Cy Cuts at: 2507 2543 2507 Size: 36 5524 EcoRI G'AATT_C Cuts at: 2869 3247 2869 Size: 378 5182 FspI TGC'GCA Cuts at: 4066 4066 Size: 5560 HaeII r_GCGC'y Cuts at: 1678 2814 4707 1678 Size: 1136 1893 2531 Fragments arranged by size: 2531 1893 1136 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2439 2471 4201 4233 4275 Size: 32 135 32 867 32 1730 32 42 Cuts at: 4275 4307 1373 Size: 32 2626 Fragments arranged by size: 2626 1730 867 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2904 3150 669 Size: 988 281 966 246 3079 Fragments arranged by size: 3079 988 966 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5560 HpaI GTT'AAC Cuts at: 3150 3150 Size: 5560 KasI G'GCGC_C Cuts at: 2810 2810 Size: 5560 KpnI G_GTAC'C Cuts at: 2780 2885 2780 Size: 105 5455 MfeI C'AATT_G Cuts at: 3159 3159 Size: 5560 MluI A'CGCG_T Cuts at: 5436 5436 Size: 5560 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4674 4858 1305 Size: 3369 184 2007 Fragments arranged by size: 3369 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2120 10 Size: 54 1425 343 288 3450 Fragments arranged by size: 3450 1425 343 288 54 MslI CAynn'nnrTG Cuts at: 518 1166 2240 2555 2622 3518 3877 4036 518 Size: 648 1074 315 67 896 359 159 2042 Fragments arranged by size: 2042 1074 896 648 359 315 159 67 MspA1I CmG'CkG Cuts at: 1606 2034 2182 2215 2224 2654 2976 3653 4801 Size: 428 148 33 9 430 322 677 1148 Cuts at: 4801 5046 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 430 428 322 245 148 33 9 NarI GG'CG_CC Cuts at: 2811 2811 Size: 5560 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4069 Fragments arranged by size: 4069 939 552 NdeI CA'TA_TG Cuts at: 387 2623 387 Size: 2236 3324 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5560 NotI GC'GGCC_GC Cuts at: 3007 3007 Size: 5560 NspI r_CATG'y Cuts at: 1132 1229 2918 4463 1132 Size: 97 1689 1545 2229 Fragments arranged by size: 2229 1689 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5560 PciI A'CATG_T Cuts at: 4459 4459 Size: 5560 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5560 PfoI T'CCnGG_A Cuts at: 2472 2472 Size: 5560 PmeI GTTT'AAAC Cuts at: 2864 2864 Size: 5560 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3629 3661 5142 5174 3629 Size: 32 1481 32 4015 Fragments arranged by size: 4015 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2507 2543 2507 Size: 36 5524 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5560 PsiI TTA'TAA Cuts at: 3130 3130 Size: 5560 PstI C_TGCA'G Cuts at: 830 2754 2912 830 Size: 1924 158 3478 Fragments arranged by size: 3478 1924 158 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5560 PvuII CAG'CTG Cuts at: 2182 2654 2182 Size: 472 5088 SalI G'TCGA_C Cuts at: 1936 2902 1936 Size: 966 4594 SanDI GG'GwC_CC Cuts at: 2507 2507 Size: 5560 SbfI CC_TGCA'GG Cuts at: 2912 2912 Size: 5560 ScaI AGT'ACT Cuts at: 3808 5520 3808 Size: 1712 3848 SfcI C'TryA_G Cuts at: 826 1045 2177 2750 2908 4043 4724 4915 826 Size: 219 1132 573 158 1135 681 191 1471 Fragments arranged by size: 1471 1135 1132 681 573 219 191 158 SfoI GGC'GCC Cuts at: 2812 2812 Size: 5560 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5560 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5560 SmaI CCC'GGG Cuts at: 1872 2887 1872 Size: 1015 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3664 4565 4827 820 Size: 197 926 1721 901 262 1553 Fragments arranged by size: 1721 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3351 493 Size: 2858 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5560 SphI G_CATG'C Cuts at: 1132 2918 1132 Size: 1786 3774 SspI AAT'ATT Cuts at: 5 52 3484 5 Size: 47 3432 2081 Fragments arranged by size: 3432 2081 47 StuI AGG'CCT Cuts at: 1517 2421 1517 Size: 904 4656 StyI C'CwwG_G Cuts at: 513 1065 2004 2248 2999 513 Size: 552 939 244 751 3074 Fragments arranged by size: 3074 939 751 552 244 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2634 3587 3604 3757 3942 4387 325 Size: 1255 1054 953 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1054 953 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3806 5518 96 Size: 274 80 33 51 1443 1829 1712 138 Fragments arranged by size: 1829 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5520 5552 5520 Size: 32 5528 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5560 XbaI T'CTAG_A Cuts at: 2896 2896 Size: 5560 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5560 XmaI C'CCGG_G Cuts at: 1870 2885 1870 Size: 1015 4545 XmnI GAAnn'nnTTC Cuts at: 3689 3689 Size: 5560 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3368 276 Size: 53 83 186 936 1834 2468 Fragments arranged by size: 2468 1834 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI XcmI