Restriction map for
pFN21AE6663
(Linear) (Six-base) MAPSORT of: KIEE6663.seq Check: 6061 from: 1 to: 855
KIEE6663.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 521 855
Size: 521 334
Acc65I G'GTAC_C
Cuts at: 0 771 855
Size: 771 84
AflIII A'CryG_T
Cuts at: 0 461 855
Size: 461 394
AleI CACnn'nnGTG
Cuts at: 0 235 550 855
Size: 235 315 305
Fragments arranged by size:
315 305 235
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 334 855
Size: 334 521
AlwNI CAG_nnn'CTG
Cuts at: 0 652 855
Size: 652 203
AvaI C'yCGr_G
Cuts at: 0 498 855
Size: 498 357
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 755 763 788 796 855
Size: 755 8 25 8 59
Fragments arranged by size:
755 59 25 8 8
BanI G'GyrC_C
Cuts at: 0 771 805 855
Size: 771 34 50
Fragments arranged by size:
771 50 34
BanII G_rGCy'C
Cuts at: 0 280 335 815 855
Size: 280 55 480 40
Fragments arranged by size:
480 280 55 40
BbeI G_GCGC'C
Cuts at: 0 809 855
Size: 809 46
BglI GCCn_nnn'nGGC
Cuts at: 0 580 855
Size: 580 275
BlpI GC'TnA_GC
Cuts at: 0 814 835 855
Size: 814 21 20
Fragments arranged by size:
814 21 20
Bme1580I G_kGCm'C
Cuts at: 0 692 855
Size: 692 163
BmrI ACTGGGnnnn_n'
Cuts at: 0 175 855
Size: 175 680
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 299 855
Size: 299 556
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 814 855
Size: 294 520 41
Fragments arranged by size:
520 294 41
BsaI GGTCTCn'nnnn_
Cuts at: 0 526 855
Size: 526 329
BsaHI Gr'CG_yC
Cuts at: 0 7 70 806 855
Size: 7 63 736 49
Fragments arranged by size:
736 63 49 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 211 455 855
Size: 211 244 400
Fragments arranged by size:
400 244 211
BseYI C'CCAG_C
Cuts at: 0 21 141 210 855
Size: 21 120 69 645
Fragments arranged by size:
645 120 69 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 855
Size: 688 167
BsiEI CG_ry'CG
Cuts at: 0 236 399 855
Size: 236 163 456
Fragments arranged by size:
456 236 163
BsiHKAI G_wGCw'C
Cuts at: 0 134 836 855
Size: 134 702 19
Fragments arranged by size:
702 134 19
BsmBI CGTCTCn'nnnn_
Cuts at: 0 347 486 855
Size: 347 139 369
Fragments arranged by size:
369 347 139
Bsp1286I G_dGCh'C
Cuts at: 0 134 280 335 692 815 836 855
Size: 134 146 55 357 123 21 19
Fragments arranged by size:
357 146 134 123 55 21 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 521 855
Size: 521 334
BsrBI CCG'CTC
Cuts at: 0 103 283 855
Size: 103 180 572
Fragments arranged by size:
572 180 103
BsrDI GCAATG_nn'
Cuts at: 0 202 599 855
Size: 202 397 256
Fragments arranged by size:
397 256 202
BsrFI r'CCGG_y
Cuts at: 0 394 855
Size: 394 461
BstAPI GCAn_nnn'nTGC
Cuts at: 0 138 855
Size: 138 717
BstYI r'GATC_y
Cuts at: 0 108 720 855
Size: 108 612 135
Fragments arranged by size:
612 135 108
Bsu36I CC'TnA_GG
Cuts at: 0 194 855
Size: 194 661
BtgI C'CryG_G
Cuts at: 0 708 855
Size: 708 147
BtsI GCAGTG_nn'
Cuts at: 0 605 742 855
Size: 605 137 113
Fragments arranged by size:
605 137 113
DraIII CAC_nnn'GTG
Cuts at: 0 138 855
Size: 138 717
DrdI GACnn_nn'nnGTC
Cuts at: 0 478 855
Size: 478 377
EaeI y'GGCC_r
Cuts at: 0 113 233 396 855
Size: 113 120 163 459
Fragments arranged by size:
459 163 120 113
EagI C'GGCC_G
Cuts at: 0 233 396 855
Size: 233 163 459
Fragments arranged by size:
459 233 163
EarI CTCTTCn'nnn_
Cuts at: 0 394 855
Size: 394 461
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 472 855
Size: 472 383
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 814 855
Size: 294 520 41
Fragments arranged by size:
520 294 41
EcoO109I rG'GnC_Cy
Cuts at: 0 502 538 855
Size: 502 36 317
Fragments arranged by size:
502 317 36
HaeII r_GCGC'y
Cuts at: 0 809 855
Size: 809 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 434 466 855
Size: 434 32 389
Fragments arranged by size:
434 389 32
KasI G'GCGC_C
Cuts at: 0 805 855
Size: 805 50
KpnI G_GTAC'C
Cuts at: 0 775 855
Size: 775 80
MscI TGG'CCA
Cuts at: 0 115 855
Size: 115 740
MslI CAynn'nnrTG
Cuts at: 0 235 550 617 855
Size: 235 315 67 238
Fragments arranged by size:
315 238 235 67
MspA1I CmG'CkG
Cuts at: 0 29 177 210 219 649 855
Size: 29 148 33 9 430 206
Fragments arranged by size:
430 206 148 33 29 9
NarI GG'CG_CC
Cuts at: 0 806 855
Size: 806 49
NdeI CA'TA_TG
Cuts at: 0 618 855
Size: 618 237
PfoI T'CCnGG_A
Cuts at: 0 467 855
Size: 467 388
PpuMI rG'GwC_Cy
Cuts at: 0 502 538 855
Size: 502 36 317
Fragments arranged by size:
502 317 36
PstI C_TGCA'G
Cuts at: 0 749 855
Size: 749 106
PvuII CAG'CTG
Cuts at: 0 177 649 855
Size: 177 472 206
Fragments arranged by size:
472 206 177
SanDI GG'GwC_CC
Cuts at: 0 502 855
Size: 502 353
SfcI C'TryA_G
Cuts at: 0 172 745 855
Size: 172 573 110
Fragments arranged by size:
573 172 110
SfoI GGC'GCC
Cuts at: 0 807 855
Size: 807 48
StuI AGG'CCT
Cuts at: 0 416 855
Size: 416 439
StyI C'CwwG_G
Cuts at: 0 243 855
Size: 243 612
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 629 855
Size: 629 226
Enzymes that do cut:
AarI Acc65I AflIII AleI AlfI AlwNI AvaI BaeI
BanI BanII BbeI BglI BlpI Bme1580I BmrI BplI
BpmI BsaI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstYI
Bsu36I BtgI BtsI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I HaeII Hin4I KasI KpnI MscI
MslI MspA1I NarI NdeI PfoI PpuMI PstI PvuII
SanDI SfcI SfoI StuI StyI TaqII
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AgeI AhdI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BmgBI BmtI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI
BsiWI BsmI BspEI BspHI BsrGI BssHII BssSI BstBI
BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI MfeI MluI MmeI NaeI NcoI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI