################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DsaI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoRV EcoT22I FauI FbaI FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AfaI 141 GTAC GT^AC AlwI 299 GGATC GGATC(4/5) Aor51HI 425 AGCGCT AGC^GCT Asp700I 108 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 123 GGTCC G^G[AT]CC AvrII 529 CCTAGG C^CTAGG BcnI 290 CCCGG CC^[GC]GG BfaI 530 CTAG C^TAG BglII 327 AGATCT A^GATCT BlnI 529 CCTAGG C^CTAGG BsmAI 406 GAGAC GTCTC(1/5) BsmFI 486 GTCCC GGGAC(10/14) BstEII 418 GGTTACC G^GT[ACGT]ACC BstPI 418 GGTTACC G^GT[ACGT]ACC Cac8I 223 GCTGGC GC[ACGT]^[ACGT]GC CfoI 426 GCGC GCG^C DrdI 199 GACAACATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 425 AGCGCT AGC^GCT EcoO65I 418 GGTTACC G^GT[ACGT]ACC EcoRI 478 GAATTC G^AATTC HaeII 425 AGCGCT [GA]GCGC^[CT] HapII 291 CCGG C^CGG HhaI 426 GCGC GCG^C HinP1I 426 GCGC G^CGC HincII 80 GTCAAC GT[CT]^[GA]AC HindII 80 GTCAAC GT[CT]^[GA]AC HpaII 291 CCGG C^CGG HphI 448 TCACC GGTGA(8/7) MaeI 530 CTAG C^TAG MaeIII 419 GTTAC ^GT[ACGT]AC MseI 50 TTAA T^TAA MspI 291 CCGG C^CGG NciI 290 CCCGG CC^[GC]GG RsaI 141 GTAC GT^AC SacI 364 GAGCTC GAGCT^C SfaNI 237 GATGC GCATC(5/9) StuI 542 AGGCCT AGG^CCT Tru9I 50 TTAA T^TAA XcmI 167 CCAAGGAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 108 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AciI 264 CCGC CCGC(-3/-1) 345 CCGC AcsI 11 AAATTT [GA]^AATT[CT] 478 GAATTC ApoI 11 AAATTT [GA]^AATT[CT] 478 GAATTC BanII 277 GGGCTC G[GA]GC[CT]^C 364 GAGCTC BbsI 357 GAAGAC GAAGAC(2/6) 208 GTCTTC BpuAI 357 GAAGAC GAAGAC(2/6) 208 GTCTTC BseRI 433 CTCCTC GAGGAG(10/8) 172 GAGGAG BsiYI 9 CCAAATTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 290 CCCGGAAATGG BslI 9 CCAAATTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 290 CCCGGAAATGG BsrI 63 CCAGT ACTGG(1/-1) 135 CCAGT BstYI 299 GGATCT [GA]^GATC[CT] 327 AGATCT Cfr13I 123 GGTCC G^G[ACGT]CC 132 GGGCC DpnII 300 GATC ^GATC 328 GATC EcoT14I 167 CCAAGG C^C[AT][AT]GG 529 CCTAGG MboI 300 GATC ^GATC 328 GATC MboII 357 GAAGA GAAGA(8/7) 209 TCTTC MflI 299 GGATCT [GA]^GATC[CT] 327 AGATCT MslI 247 CACCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 261 CATCCGCATG NdeII 300 GATC ^GATC 328 GATC PleI 505 GACTC GAGTC(4/5) 525 GACTC Sau3AI 300 GATC ^GATC 328 GATC Sau96I 123 GGTCC G^G[ACGT]CC 132 GGGCC StyI 167 CCAAGG C^C[AT][AT]GG 529 CCTAGG TfiI 392 GAATC G^A[AT]TC 534 GAATC Tsp509I 12 AATT ^AATT 479 AATT TspRI 64 CAGTG CA[GC]TG[ACGT][ACGT]^ 136 CAGTG XhoII 299 GGATCT [GA]^GATC[CT] 327 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AluI AspHI BbvI BmyI BsaJI BsiHKAI Bsp1286I BssKI BstF5I BstNI DdeI EcoRII Fnu4HI FokI HaeIII HinfI ItaI MnlI MvaI MwoI NlaIII ScrFI TseI