Restriction map for
pFN21AE6553
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# NO CUT ENZYMES ( 196 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AfaI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BbvI
BcgI BciVI BclI BfrI BglI
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoT14I EcoT22I
FauI FbaI Fnu4HI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI ItaI KasI
KpnI KspI MaeIII MfeI MluI
MluNI MroI MscI MslI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
TseI Tsp45I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AseI 264 ATTAAT AT^TAAT
AsnI 264 ATTAAT AT^TAAT
BcnI 290 CCGGG CC^[GC]GG
BglII 340 AGATCT A^GATCT
BpmI 220 CTGGAG CTGGAG(16/14)
BsaBI 118 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiYI 6 CCAAACCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 6 CCAAACCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 295 GAGAC GTCTC(1/5)
BsmBI 295 GAGACG CGTCTC(1/5)
BsmI 215 GAATGC GAATGC(1/-1)
BstNI 11 CCAGG CC^[AT]GG
DraII 405 AGGCCCC [GA]G^G[ACGT]CC[CT]
EarI 61 GAAGAG CTCTTC(1/4)
EcoO109I 405 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRII 11 CCAGG ^CC[AT]GG
EcoRV 196 GATATC GAT^ATC
HapII 290 CCGG C^CGG
HindIII 32 AAGCTT A^AGCTT
HpaII 290 CCGG C^CGG
HphI 246 TCACC GGTGA(8/7)
Ksp632I 61 GAAGAG CTCTTC(1/4)
MaeII 298 ACGT A^CGT
MamI 118 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 61 GAAGA GAAGA(8/7)
MspI 290 CCGG C^CGG
MvaI 11 CCAGG CC^[AT]GG
MwoI 415 GCCAAGAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 290 CCGGG CC^[GC]GG
PshBI 264 ATTAAT AT^TAAT
SfaNI 327 GATGC GCATC(5/9)
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BfaI 94 CTAG C^TAG
250 CTAG
BsaJI 11 CCAGGG C^C[ACGT][ACGT]GG
290 CCGGGG
BsmFI 204 GTCCC GGGAC(10/14)
235 GTCCC
BsrI 138 ACTGG ACTGG(1/-1)
281 CCAGT
BssKI 11 CCAGG ^CC[ACGT]GG
290 CCGGG
BstYI 123 GGATCT [GA]^GATC[CT]
340 AGATCT
Cfr13I 51 GGCCC G^G[ACGT]CC
406 GGCCC
DdeI 319 CTCAG C^T[ACGT]AG
431 CTGAG
HaeIII 51 GGCC GG^CC
406 GGCC
HinfI 233 GAGTC G^A[ACGT]TC
243 GACTC
MaeI 94 CTAG C^TAG
250 CTAG
MflI 123 GGATCT [GA]^GATC[CT]
340 AGATCT
MseI 176 TTAA T^TAA
265 TTAA
NlaIII 162 CATG CATG^
201 CATG
NlaIV 286 GGCTCC GG[ACGT]^[ACGT]CC
406 GGCCCC
PleI 243 GACTC GAGTC(4/5)
233 GAGTC
Sau96I 51 GGCCC G^G[ACGT]CC
406 GGCCC
ScrFI 11 CCAGG CC^[ACGT]GG
290 CCGGG
Tru9I 176 TTAA T^TAA
265 TTAA
TspRI 114 CAGTG CA[GC]TG[ACGT][ACGT]^
282 CAGTG
XhoII 123 GGATCT [GA]^GATC[CT]
340 AGATCT
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
#########################################
AluI AlwI BstF5I DpnII FokI
MboI MnlI NdeII Sau3AI Tsp509I