################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstF5I BstUI BstXI BstZ17I CelII CfoI ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MroI MseI MslI MspA1I MunI MvnI NarI NcoI NdeI NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AfaI 402 GTAC GT^AC AluI 17 AGCT AG^CT ApaI 135 GGGCCC GGGCC^C AvaI 392 CCCGGG C^[CT]CG[GA]G BalI 160 TGGCCA TGG^CCA BanI 168 GGCACC G^G[CT][GA]CC BanII 135 GGGCCC G[GA]GC[CT]^C BbvI 65 GCAGC GCAGC(8/12) BsaI 221 GAGACC GGTCTC(1/5) BsgI 43 CTGCAC GTGCAG(16/14) BsmAI 221 GAGAC GTCTC(1/5) BsoBI 392 CCCGGG C^[CT]CG[GA]G BspMI 224 ACCTGC ACCTGC(4/8) BsrDI 125 CATTGC GCAATG(2/0) BsrFI 303 GCCGGC [GA]^CCGG[CT] BstEII 151 GGTGACC G^GT[ACGT]ACC BstPI 151 GGTGACC G^GT[ACGT]ACC Cfr10I 303 GCCGGC [GA]^CCGG[CT] EarI 397 GAAGAG CTCTTC(1/4) EcoNI 157 CCTTGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 151 GGTGACC G^GT[ACGT]ACC Fnu4HI 65 GCAGC GC^[ACGT]GC HinfI 239 GAGTC G^A[ACGT]TC ItaI 65 GCAGC GC^[ACGT]GC Ksp632I 397 GAAGAG CTCTTC(1/4) MaeIII 152 GTGAC ^GT[ACGT]AC MluNI 160 TGGCCA TGG^CCA MscI 160 TGGCCA TGG^CCA NaeI 303 GCCGGC GCC^GGC NgoMIV 303 GCCGGC G^CCGGC PleI 239 GAGTC GAGTC(4/5) PpuMI 309 AGGACCC [GA]G^G[AT]CC[CT] RsaI 402 GTAC GT^AC SfaNI 56 GCATC GCATC(5/9) SmaI 392 CCCGGG CCC^GGG TseI 65 GCAGC G^C[AT]GC Tsp45I 152 GTGAC ^GT[GC]AC XcmI 322 CCAGAAAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 392 CCCGGG C^CCGGG XmaI 392 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### BamHI 26 GGATCC G^GATCC 267 GGATCC BfaI 260 CTAG C^TAG 382 CTAG BmyI 135 GGGCCC G[AGT]GC[ACT]^C 167 GGGCAC BpmI 216 CTGGAG CTGGAG(16/14) 236 CTGGAG BsmFI 145 GTCCC GGGAC(10/14) 212 GTCCC Bsp1286I 135 GGGCCC G[AGT]GC[ACT]^C 167 GGGCAC EaeI 160 TGGCCA [CT]^GGCC[GA] 301 TGGCCG EcoT14I 157 CCTTGG C^C[AT][AT]GG 298 CCTTGG FauI 91 CCCGC CCCGC(4/6) 200 CCCGC HphI 151 GGTGA GGTGA(8/7) 84 TCACC MaeI 260 CTAG C^TAG 382 CTAG MboII 184 GAAGA GAAGA(8/7) 397 GAAGA MwoI 56 GCATCTCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 65 GCAGCGAGGGC StyI 157 CCTTGG C^C[AT][AT]GG 298 CCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AciI AlwI AocI AvaII BcnI BsaJI BseRI BsiYI BslI BsrI BssKI BstNI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraII Eco81I EcoO109I EcoRII HaeIII HapII HpaII MboI MflI MnlI MspI MvaI NciI NdeII NlaIV Sau3AI Sau96I ScrFI XhoII