(Circular) (Six-base) MAPSORT of: pFN21AE6501.seq Check: 7552 from: 1 to: 5113 pFN21AE6501 5113 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2923 278 Size: 53 83 186 936 1387 2468 Fragments arranged by size: 2468 1387 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2456 1937 Size: 519 4594 Acc65I G'GTAC_C Cuts at: 2434 2434 Size: 5113 AclI AA'CG_TT Cuts at: 1563 3240 3613 1563 Size: 1677 373 3063 Fragments arranged by size: 3063 1677 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3176 4560 4828 1632 Size: 1544 1384 268 1917 Fragments arranged by size: 1917 1544 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4916 AflIII A'CryG_T Cuts at: 1203 4012 4989 1203 Size: 2809 977 1327 Fragments arranged by size: 2809 1327 977 AgeI A'CCGG_T Cuts at: 3944 3944 Size: 5113 AhdI GACnn_n'nnGTC Cuts at: 3842 3842 Size: 5113 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5113 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5113 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2260 2292 2260 Size: 32 5081 AlwNI CAG_nnn'CTG Cuts at: 2856 4428 2856 Size: 1572 3541 ApaI G_GGCC'C Cuts at: 2144 2144 Size: 5113 ApaLI G'TGCA_C Cuts at: 3170 4326 3170 Size: 1156 3957 ApoI r'AATT_y Cuts at: 1493 2422 2648 2800 4909 1493 Size: 929 226 152 2109 1697 Fragments arranged by size: 2109 1697 929 226 152 AseI AT'TA_AT Cuts at: 160 1032 3667 160 Size: 872 2635 1606 Fragments arranged by size: 2635 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5113 AvaI C'yCGr_G Cuts at: 1870 1943 2397 2438 1870 Size: 73 454 41 4545 Fragments arranged by size: 4545 454 73 41 BamHI G'GATC_C Cuts at: 2031 2272 2443 2810 2031 Size: 241 171 367 4334 Fragments arranged by size: 4334 367 241 171 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2173 2434 3889 618 Size: 325 217 627 386 261 1455 1842 Fragments arranged by size: 1842 1455 627 386 325 261 217 BanII G_rGCy'C Cuts at: 1391 2144 2432 1391 Size: 753 288 4072 Fragments arranged by size: 4072 753 288 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4142 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3304 3338 1903 Size: 34 1367 34 3678 Fragments arranged by size: 3678 1367 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3005 4215 3005 Size: 1210 3903 BclI T'GATC_A Cuts at: 1548 1548 Size: 5113 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3724 136 Size: 107 122 71 631 605 2052 1525 Fragments arranged by size: 2052 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5108 5108 Size: 5113 BlpI GC'TnA_GC Cuts at: 2530 2530 Size: 5113 Bme1580I G_kGCm'C Cuts at: 1790 2144 2176 3174 4330 1790 Size: 354 32 998 1156 2573 Fragments arranged by size: 2573 1156 998 354 32 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5113 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2141 2205 2844 Size: 416 53 83 399 24 724 64 639 Cuts at: 2844 3802 442 Size: 958 1753 Fragments arranged by size: 1753 958 724 639 416 399 83 64 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5113 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2137 2267 2137 Size: 130 4983 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2242 2262 3773 1239 Size: 1003 20 1511 2579 Fragments arranged by size: 2579 1511 1003 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3238 4103 4401 3238 Size: 865 298 3950 Fragments arranged by size: 3950 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2220 3776 882 Size: 617 721 1556 2219 Fragments arranged by size: 2219 1556 721 617 BsaAI yAC'GTr Cuts at: 493 1204 2904 493 Size: 711 1700 2702 Fragments arranged by size: 2702 1700 711 BsaBI GATnn'nnATC Cuts at: 5107 5107 Size: 5113 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2920 3302 3937 275 Size: 53 83 186 936 1387 382 635 1451 Fragments arranged by size: 1451 1387 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3546 3944 4218 4365 4776 3546 Size: 398 274 147 411 3883 Fragments arranged by size: 3883 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2260 2290 1178 Size: 30 165 30 857 30 4001 Fragments arranged by size: 4001 857 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2126 2268 2390 1186 Size: 940 142 122 3909 Fragments arranged by size: 3909 940 142 122 BseYI C'CCAG_C Cuts at: 2319 4316 4812 2319 Size: 1997 496 2620 Fragments arranged by size: 2620 1997 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2033 1163 Size: 573 297 4243 Fragments arranged by size: 4243 573 297 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2563 3324 4352 1540 Size: 275 186 562 761 1028 2301 Fragments arranged by size: 2301 1028 761 562 275 186 BsiHKAI G_wGCw'C Cuts at: 3174 3259 4330 3174 Size: 85 1071 3957 Fragments arranged by size: 3957 1071 85 BsmI GAATG_Cn' Cuts at: 2624 2717 2624 Size: 93 5020 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2144 2176 2432 3174 3259 4330 1391 Size: 399 354 32 256 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 354 256 85 32 BspEI T'CCGG_A Cuts at: 4776 4776 Size: 5113 BspHI T'CATG_A Cuts at: 3002 4732 3002 Size: 1730 3383 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2238 2468 844 Size: 1394 230 3489 Fragments arranged by size: 3489 1394 230 BsrBI CCG'CTC Cuts at: 1394 1823 3000 1394 Size: 429 1177 3507 Fragments arranged by size: 3507 1177 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2129 3608 3782 65 Size: 1183 881 1479 174 1396 Fragments arranged by size: 1479 1396 1183 881 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2308 3757 3944 1700 Size: 110 498 1449 187 2869 Fragments arranged by size: 2869 1449 498 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5113 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5113 BssSI C'ACGA_G Cuts at: 3173 4185 3173 Size: 1012 4101 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5113 BstBI TT'CG_AA Cuts at: 3917 3917 Size: 5113 BstEII G'GTnAC_C Cuts at: 1184 2156 1184 Size: 972 4141 BstYI r'GATC_y Cuts at: 1972 2031 2191 2272 2443 2810 3195 3212 4664 Size: 59 160 81 171 367 385 17 1452 Cuts at: 4664 4750 4762 5108 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 171 160 86 81 59 17 12 Bsu36I CC'TnA_GG Cuts at: 2136 2352 2370 2136 Size: 216 18 4879 Fragments arranged by size: 4879 216 18 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4561 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4374 Fragments arranged by size: 4374 683 56 BtsI GCAGTG_nn' Cuts at: 2629 3423 3443 2629 Size: 794 20 4299 Fragments arranged by size: 4299 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5113 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5078 DraI TTT'AAA Cuts at: 2417 2417 Size: 5113 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4120 809 Size: 507 2804 1802 Fragments arranged by size: 2804 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2165 2306 2560 Size: 54 1425 50 275 18 335 141 254 Cuts at: 2560 3449 5078 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 335 275 254 141 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2560 1537 Size: 275 748 4090 Fragments arranged by size: 4090 748 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2397 3043 1353 Size: 1044 646 3423 Fragments arranged by size: 3423 1044 646 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2187 3700 4074 4220 387 Size: 1064 138 598 1513 374 146 1280 Fragments arranged by size: 1513 1280 1064 598 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2242 2262 3176 3773 4560 4828 1239 Size: 393 610 20 914 597 787 268 1524 Fragments arranged by size: 1524 914 787 610 597 393 268 20 EcoNI CCTnn'n_nnAGG Cuts at: 2166 2166 Size: 5113 EcoO109I rG'GnC_Cy Cuts at: 2140 2141 2315 2140 Size: 1 174 4938 Fragments arranged by size: 4938 174 1 EcoRI G'AATT_C Cuts at: 2422 2800 2422 Size: 378 4735 FspI TGC'GCA Cuts at: 3619 3619 Size: 5113 HaeII r_GCGC'y Cuts at: 1678 4260 1678 Size: 2582 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3754 3786 3828 3860 1373 Size: 32 135 32 2182 32 42 32 2626 Fragments arranged by size: 2626 2182 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2457 2703 669 Size: 988 281 519 246 3079 Fragments arranged by size: 3079 988 519 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5113 HpaI GTT'AAC Cuts at: 2703 2703 Size: 5113 KpnI G_GTAC'C Cuts at: 2438 2438 Size: 5113 MfeI C'AATT_G Cuts at: 2712 2712 Size: 5113 MluI A'CGCG_T Cuts at: 4989 4989 Size: 5113 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4227 4411 1305 Size: 2922 184 2007 Fragments arranged by size: 2922 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2167 10 Size: 54 1425 343 335 2956 Fragments arranged by size: 2956 1425 343 335 54 MslI CAynn'nnrTG Cuts at: 518 1166 3071 3430 3589 518 Size: 648 1905 359 159 2042 Fragments arranged by size: 2042 1905 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2529 3206 4354 4599 1606 Size: 923 677 1148 245 2120 Fragments arranged by size: 2120 1148 923 677 245 NaeI GCC'GGC Cuts at: 2310 2310 Size: 5113 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4561 NdeI CA'TA_TG Cuts at: 387 387 Size: 5113 NgoMIV G'CCGG_C Cuts at: 2308 2308 Size: 5113 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5113 NotI GC'GGCC_GC Cuts at: 2560 2560 Size: 5113 NspI r_CATG'y Cuts at: 1132 1229 2471 4016 1132 Size: 97 1242 1545 2229 Fragments arranged by size: 2229 1545 1242 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5113 PciI A'CATG_T Cuts at: 4012 4012 Size: 5113 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5113 PmeI GTTT'AAAC Cuts at: 2417 2417 Size: 5113 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2260 2292 3182 3214 4695 4727 2260 Size: 32 890 32 1481 32 2646 Fragments arranged by size: 2646 1481 890 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2315 2315 Size: 5113 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5113 PsiI TTA'TAA Cuts at: 2683 2683 Size: 5113 PspOMI G'GGCC_C Cuts at: 2140 2140 Size: 5113 PstI C_TGCA'G Cuts at: 830 2465 830 Size: 1635 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5113 SalI G'TCGA_C Cuts at: 1936 2455 1936 Size: 519 4594 SbfI CC_TGCA'GG Cuts at: 2465 2465 Size: 5113 ScaI AGT'ACT Cuts at: 3361 5073 3361 Size: 1712 3401 SfcI C'TryA_G Cuts at: 826 1045 2461 3596 4277 4468 826 Size: 219 1416 1135 681 191 1471 Fragments arranged by size: 1471 1416 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5113 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5113 SmaI CCC'GGG Cuts at: 1872 2399 2440 1872 Size: 527 41 4545 Fragments arranged by size: 4545 527 41 SmlI C'TyrA_G Cuts at: 820 1017 1943 3217 4118 4380 820 Size: 197 926 1274 901 262 1553 Fragments arranged by size: 1553 1274 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2904 493 Size: 2411 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5113 SphI G_CATG'C Cuts at: 1132 2471 1132 Size: 1339 3774 SspI AAT'ATT Cuts at: 5 52 3037 5 Size: 47 2985 2081 Fragments arranged by size: 2985 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5113 StyI C'CwwG_G Cuts at: 513 1065 2162 2303 2552 513 Size: 552 1097 141 249 3074 Fragments arranged by size: 3074 1097 552 249 141 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2106 2121 3140 3157 3310 3495 3940 Size: 1255 526 15 1019 17 153 185 445 Cuts at: 3940 325 Size: 1498 Fragments arranged by size: 1498 1255 1019 526 445 185 153 17 15 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2406 3359 5071 Size: 274 80 33 51 1443 429 953 1712 Cuts at: 5071 96 Size: 138 Fragments arranged by size: 1712 1443 953 429 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5073 5105 5073 Size: 32 5081 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5113 XbaI T'CTAG_A Cuts at: 2449 2449 Size: 5113 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2334 2334 Size: 5113 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5113 XmaI C'CCGG_G Cuts at: 1870 2397 2438 1870 Size: 527 41 4545 Fragments arranged by size: 4545 527 41 XmnI GAAnn'nnTTC Cuts at: 3242 3242 Size: 5113 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2921 276 Size: 53 83 186 936 1387 2468 Fragments arranged by size: 2468 1387 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI