Restriction map for
pFN21AE2319
(Circular) (Six-base) MAPSORT of: pFN21AE2319.seq Check: 2886 from: 1 to: 5704
pFN21AE2319 5704 bp
With 182 enzymes: SgfI *
January 11, 2011 14:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3514 278
Size: 53 83 186 936 1978 2468
Fragments arranged by size:
2468 1978 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3047 1937
Size: 1110 4594
Acc65I G'GTAC_C
Cuts at: 3025 3025
Size: 5704
AclI AA'CG_TT
Cuts at: 1563 3831 4204 1563
Size: 2268 373 3063
Fragments arranged by size:
3063 2268 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2753 3767 5151 5419 1632
Size: 1121 1014 1384 268 1917
Fragments arranged by size:
1917 1384 1121 1014 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5507
AflIII A'CryG_T
Cuts at: 1203 4603 5580 1203
Size: 3400 977 1327
Fragments arranged by size:
3400 1327 977
AgeI A'CCGG_T
Cuts at: 4535 4535
Size: 5704
AhdI GACnn_n'nnGTC
Cuts at: 4433 4433
Size: 5704
AleI CACnn'nnGTG
Cuts at: 1166 2625 1166
Size: 1459 4245
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5704
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2018 2032 2050 2000
Size: 18 14 18 5654
Fragments arranged by size:
5654 18 18 14
AlwNI CAG_nnn'CTG
Cuts at: 2068 2491 2875 3447 5019 2068
Size: 423 384 572 1572 2753
Fragments arranged by size:
2753 1572 572 423 384
ApaI G_GGCC'C
Cuts at: 2773 2773
Size: 5704
ApaLI G'TGCA_C
Cuts at: 3761 4917 3761
Size: 1156 4548
ApoI r'AATT_y
Cuts at: 1493 2498 2726 3013 3239 3391 5500 1493
Size: 1005 228 287 226 152 2109 1697
Fragments arranged by size:
2109 1697 1005 287 228 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4258 160
Size: 872 3226 1606
Fragments arranged by size:
3226 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
AvaI C'yCGr_G
Cuts at: 1870 1943 3029 1870
Size: 73 1086 4545
Fragments arranged by size:
4545 1086 73
BamHI G'GATC_C
Cuts at: 3034 3401 3034
Size: 367 5337
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3025 4480 618
Size: 325 217 627 1238 1455 1842
Fragments arranged by size:
1842 1455 1238 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2773 3023 1391
Size: 1382 250 4072
Fragments arranged by size:
4072 1382 250
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4733
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3895 3929 1903
Size: 34 1958 34 3678
Fragments arranged by size:
3678 1958 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2297 3596 4806 2297
Size: 1299 1210 3195
Fragments arranged by size:
3195 1299 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5704
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2264 2755 4315
Size: 107 122 71 631 605 592 491 1560
Cuts at: 4315 136
Size: 1525
Fragments arranged by size:
1560 1525 631 605 592 491 122 107
71
BglII A'GATC_T
Cuts at: 5699 5699
Size: 5704
BlpI GC'TnA_GC
Cuts at: 3121 3121
Size: 5704
Bme1580I G_kGCm'C
Cuts at: 1790 2773 3765 4921 1790
Size: 983 992 1156 2573
Fragments arranged by size:
2573 1156 992 983
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5704
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2162 3435 4393
Size: 416 53 83 399 24 745 1273 958
Cuts at: 4393 442
Size: 1753
Fragments arranged by size:
1753 1273 958 745 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5704
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2631 2864 2631
Size: 233 5471
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2543 2643 2775 2797 4364 1239
Size: 1304 100 132 22 1567 2579
Fragments arranged by size:
2579 1567 1304 132 100 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3829 4694 4992 3829
Size: 865 298 4541
Fragments arranged by size:
4541 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2519 4367 882
Size: 617 1020 1848 2219
Fragments arranged by size:
2219 1848 1020 617
BsaAI yAC'GTr
Cuts at: 493 1204 3495 493
Size: 711 2291 2702
Fragments arranged by size:
2702 2291 711
BsaBI GATnn'nnATC
Cuts at: 5698 5698
Size: 5704
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3511 3893 4528 275
Size: 53 83 186 936 1978 382 635 1451
Fragments arranged by size:
1978 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4137 4535 4809 4956 5367 4137
Size: 398 274 147 411 4474
Fragments arranged by size:
4474 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2000 2030 1178
Size: 30 165 30 597 30 4852
Fragments arranged by size:
4852 597 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2701 3005 1186
Size: 1515 304 3885
Fragments arranged by size:
3885 1515 304
BseYI C'CCAG_C
Cuts at: 2077 2950 4907 5403 2077
Size: 873 1957 496 2378
Fragments arranged by size:
2378 1957 873 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5131
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2264 3154 3915 4943 1540
Size: 275 186 263 890 761 1028 2301
Fragments arranged by size:
2301 1028 890 761 275 263 186
BsiHKAI G_wGCw'C
Cuts at: 3765 3850 4921 3765
Size: 85 1071 4548
Fragments arranged by size:
4548 1071 85
BsmI GAATG_Cn'
Cuts at: 3215 3308 3215
Size: 93 5611
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2773 3023 3765 3850 4921 1391
Size: 399 983 250 742 85 1071 2174
Fragments arranged by size:
2174 1071 983 742 399 250 85
BspEI T'CCGG_A
Cuts at: 5367 5367
Size: 5704
BspHI T'CATG_A
Cuts at: 3593 5323 3593
Size: 1730 3974
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3059 844
Size: 2215 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2044 2189 2811 2985 3591 1394
Size: 429 221 145 622 174 606 3507
Fragments arranged by size:
3507 622 606 429 221 174 145
BsrDI GCAATG_nn'
Cuts at: 65 1248 4199 4373 65
Size: 1183 2951 174 1396
Fragments arranged by size:
2951 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2104 2263 4348 4535 1700
Size: 110 294 159 2085 187 2869
Fragments arranged by size:
2869 2085 294 187 159 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5704
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5704
BssSI C'ACGA_G
Cuts at: 2625 3764 4776 2625
Size: 1139 1012 3553
Fragments arranged by size:
3553 1139 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2875 794
Size: 2081 3623
BstBI TT'CG_AA
Cuts at: 4508 4508
Size: 5704
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5704
BstXI CCAn_nnnn'nTGG
Cuts at: 2408 2821 2408
Size: 413 5291
BstYI r'GATC_y
Cuts at: 1972 3034 3401 3786 3803 5255 5341 5353 5699
Size: 1062 367 385 17 1452 86 12 346
Cuts at: 5699 1972
Size: 1977
Fragments arranged by size:
1977 1452 1062 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2069 2069
Size: 5704
BtgI C'CryG_G
Cuts at: 513 1065 2004 2401 2407 513
Size: 552 939 397 6 3810
Fragments arranged by size:
3810 939 552 397 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4965
Fragments arranged by size:
4965 683 56
BtsI GCAGTG_nn'
Cuts at: 3220 4014 4034 3220
Size: 794 20 4890
Fragments arranged by size:
4890 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5704
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5669
DraI TTT'AAA
Cuts at: 3008 3008
Size: 5704
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4711 809
Size: 507 3395 1802
Fragments arranged by size:
3395 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2404 3151 4040
Size: 54 1425 50 275 18 574 747 889
Cuts at: 4040 5669 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 747 574 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3151 1537
Size: 275 1339 4090
Fragments arranged by size:
4090 1339 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2426 3634 1353
Size: 1073 1208 3423
Fragments arranged by size:
3423 1208 1073
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2840 4291 4665 4811 387
Size: 1064 138 1251 1451 374 146 1280
Fragments arranged by size:
1451 1280 1251 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2543 2643 2753 2775 2797 3767 4364
Size: 393 911 100 110 22 22 970 597
Cuts at: 4364 5151 5419 1239
Size: 787 268 1524
Fragments arranged by size:
1524 970 911 787 597 393 268 110
100 22 22
EcoNI CCTnn'n_nnAGG
Cuts at: 2605 2605
Size: 5704
EcoO109I rG'GnC_Cy
Cuts at: 2073 2769 2770 2843 2073
Size: 696 1 73 4934
Fragments arranged by size:
4934 696 73 1
EcoRI G'AATT_C
Cuts at: 3013 3391 3013
Size: 378 5326
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2332 2332
Size: 5704
FspI TGC'GCA
Cuts at: 4210 4210
Size: 5704
HaeII r_GCGC'y
Cuts at: 1678 4851 1678
Size: 3173 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2465 2497 4345 4377 4419
Size: 32 135 32 893 32 1848 32 42
Cuts at: 4419 4451 1373
Size: 32 2626
Fragments arranged by size:
2626 1848 893 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3048 3294 669
Size: 988 281 1110 246 3079
Fragments arranged by size:
3079 1110 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5704
HpaI GTT'AAC
Cuts at: 3294 3294
Size: 5704
KpnI G_GTAC'C
Cuts at: 3029 3029
Size: 5704
MfeI C'AATT_G
Cuts at: 2469 3303 2469
Size: 834 4870
MluI A'CGCG_T
Cuts at: 5580 5580
Size: 5704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2281 4818 5002 1305
Size: 976 2537 184 2007
Fragments arranged by size:
2537 2007 976 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2406 10
Size: 54 1425 343 574 3308
Fragments arranged by size:
3308 1425 574 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2406 2625 3662 4021 4180 518
Size: 648 1240 219 1037 359 159 2042
Fragments arranged by size:
2042 1240 1037 648 359 219 159
MspA1I CmG'CkG
Cuts at: 1606 2654 3120 3797 4945 5190 1606
Size: 1048 466 677 1148 245 2120
Fragments arranged by size:
2120 1148 1048 677 466 245
NaeI GCC'GGC
Cuts at: 2106 2106
Size: 5704
NcoI C'CATG_G
Cuts at: 513 1065 2004 2401 2407 513
Size: 552 939 397 6 3810
Fragments arranged by size:
3810 939 552 397 6
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5704
NgoMIV G'CCGG_C
Cuts at: 2104 2104
Size: 5704
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5704
NotI GC'GGCC_GC
Cuts at: 3151 3151
Size: 5704
NspI r_CATG'y
Cuts at: 1132 1229 3062 4607 1132
Size: 97 1833 1545 2229
Fragments arranged by size:
2229 1833 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2939 1348
Size: 1591 4113
PciI A'CATG_T
Cuts at: 4603 4603
Size: 5704
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5704
PfoI T'CCnGG_A
Cuts at: 2037 2367 2037
Size: 330 5374
PmeI GTTT'AAAC
Cuts at: 3008 3008
Size: 5704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2000 2032 3773 3805 5286 5318 2000
Size: 32 1741 32 1481 32 2386
Fragments arranged by size:
2386 1741 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2843 2843
Size: 5704
PshAI GACnn'nnGTC
Cuts at: 1613 2531 1613
Size: 918 4786
PsiI TTA'TAA
Cuts at: 3274 3274
Size: 5704
PspOMI G'GGCC_C
Cuts at: 2769 2769
Size: 5704
PstI C_TGCA'G
Cuts at: 830 2638 3056 830
Size: 1808 418 3478
Fragments arranged by size:
3478 1808 418
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5704
SalI G'TCGA_C
Cuts at: 1936 3046 1936
Size: 1110 4594
SbfI CC_TGCA'GG
Cuts at: 3056 3056
Size: 5704
ScaI AGT'ACT
Cuts at: 3952 5664 3952
Size: 1712 3992
SfcI C'TryA_G
Cuts at: 826 1045 2120 2634 3052 4187 4868 5059 826
Size: 219 1075 514 418 1135 681 191 1471
Fragments arranged by size:
1471 1135 1075 681 514 418 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5704
SmaI CCC'GGG
Cuts at: 1872 3031 1872
Size: 1159 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3808 4709 4971 820
Size: 197 926 1865 901 262 1553
Fragments arranged by size:
1865 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3495 493
Size: 3002 2702
SpeI A'CTAG_T
Cuts at: 152 2198 152
Size: 2046 3658
SphI G_CATG'C
Cuts at: 1132 3062 1132
Size: 1930 3774
SspI AAT'ATT
Cuts at: 5 52 3628 5
Size: 47 3576 2081
Fragments arranged by size:
3576 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5704
StyI C'CwwG_G
Cuts at: 513 1065 2004 2169 2401 2407 2753 3143 513
Size: 552 939 165 232 6 346 390 3074
Fragments arranged by size:
3074 939 552 390 346 232 165 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2490 2692 2736 2814 3731 3748 3901
Size: 1255 910 202 44 78 917 17 153
Cuts at: 3901 4086 4531 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 917 910 445 202 185 153
78 44 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2320 3950 5662
Size: 274 80 33 51 1443 343 1630 1712
Cuts at: 5662 96
Size: 138
Fragments arranged by size:
1712 1630 1443 343 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2349 2381 5664 5696 2349
Size: 32 3283 32 2357
Fragments arranged by size:
3283 2357 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5704
XbaI T'CTAG_A
Cuts at: 3040 3040
Size: 5704
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5704
XmaI C'CCGG_G
Cuts at: 1870 3029 1870
Size: 1159 4545
XmnI GAAnn'nnTTC
Cuts at: 3833 3833
Size: 5704
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3512 276
Size: 53 83 186 936 1978 2468
Fragments arranged by size:
2468 1978 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI