Restriction map for
pFN21AE4110
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AhdI Alw44I AlwI
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbsI BbvCI BcgI BciVI BclI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I CelII ClaI
CpoI DraI DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HindIII HpaI KasI
Ksp632I KspI MamI MluI MluNI
MroI MscI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 61 GGTACC G^GTACC
AcsI 658 GAATTT [GA]^AATT[CT]
AfaI 62 GTAC GT^AC
AflIII 1094 ACACGT A^C[GA][CT]GT
AgeI 804 ACCGGT A^CCGGT
ApoI 658 GAATTT [GA]^AATT[CT]
Asp718I 61 GGTACC G^GTACC
AvaI 1346 CCCGAG C^[CT]CG[GA]G
BanI 61 GGTACC G^G[CT][GA]CC
BbrPI 1095 CACGTG CAC^GTG
BglI 210 GCCCAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1095 CACGTG [CT]AC^GT[GA]
BsaWI 804 ACCGGT [AT]^CCGG[AT]
BsmBI 1233 CGTCTC CGTCTC(1/5)
BsoBI 1346 CCCGAG C^[CT]CG[GA]G
BspMI 1287 GCAGGT ACCTGC(4/8)
BstEII 1323 GGTGACC G^GT[ACGT]ACC
BstPI 1323 GGTGACC G^GT[ACGT]ACC
DraII 857 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 525 GACTCAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 857 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1323 GGTGACC G^GT[ACGT]ACC
EcoT14I 1122 CCATGG C^C[AT][AT]GG
HaeII 248 AGCGCC [GA]GCGC^[CT]
HincII 40 GTCAAC GT[CT]^[GA]AC
HindII 40 GTCAAC GT[CT]^[GA]AC
KpnI 61 GGTACC GGTAC^C
MfeI 616 CAATTG C^AATTG
MseI 449 TTAA T^TAA
MslI 1095 CACGTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 616 CAATTG C^AATTG
NcoI 1122 CCATGG C^CATGG
PinAI 804 ACCGGT A^CCGGT
PmaCI 1095 CACGTG CAC^GTG
PmlI 1095 CACGTG CAC^GTG
PstI 868 CTGCAG CTGCA^G
RsaI 62 GTAC GT^AC
SmlI 642 CTTGAG C^T[CT][GA]AG
StyI 1122 CCATGG C^C[AT][AT]GG
TaqI 86 TCGA T^CGA
Tru9I 449 TTAA T^TAA
TthHB8I 86 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AocI 66 CCTGAGG CC^T[ACGT]AGG
573 CCTGAGG
AvaII 981 GGACC G^G[AT]CC
1421 GGTCC
BglII 314 AGATCT A^GATCT
683 AGATCT
BsgI 1134 CTGCAC GTGCAG(16/14)
1162 GTGCAG
BstYI 314 AGATCT [GA]^GATC[CT]
683 AGATCT
Bsu36I 66 CCTGAGG CC^T[ACGT]AGG
573 CCTGAGG
CfoI 249 GCGC GCG^C
396 GCGC
DpnII 315 GATC ^GATC
684 GATC
DsaI 1122 CCATGG C^C[GA][CT]GG
1139 CCGTGG
EaeI 93 CGGCCA [CT]^GGCC[GA]
1189 CGGCCA
Eco81I 66 CCTGAGG CC^T[ACGT]AGG
573 CCTGAGG
EcoNI 522 CCTGACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1376 CCTTCACTAGG
FauI 122 CCCGC CCCGC(4/6)
864 CCCGC
HhaI 249 GCGC GCG^C
396 GCGC
HinP1I 249 GCGC G^CGC
396 GCGC
MaeII 230 ACGT A^CGT
1096 ACGT
MboI 315 GATC ^GATC
684 GATC
MflI 314 AGATCT [GA]^GATC[CT]
683 AGATCT
NdeII 315 GATC ^GATC
684 GATC
NlaIII 393 CATG CATG^
1123 CATG
PleI 525 GACTC GAGTC(4/5)
157 GAGTC
Sau3AI 315 GATC ^GATC
684 GATC
SfcI 423 CTATAG C^T[GA][CT]AG
868 CTGCAG
TfiI 477 GATTC G^A[AT]TC
837 GATTC
Tsp509I 617 AATT ^AATT
659 AATT
XbaI 585 TCTAGA T^CTAGA
939 TCTAGA
XhoII 314 AGATCT [GA]^GATC[CT]
683 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AspHI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI Cac8I Cfr10I Cfr13I
DdeI EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeIII MboII MnlI MspA1I
MspI MvaI MwoI NciI NlaIV
PvuII SacI Sau96I ScrFI SfaNI
TseI Tsp45I TspRI XcmI