(Circular) (Six-base) MAPSORT of: pFN21AE4110.seq Check: 3951 from: 1 to: 6148 pFN21AE4110 6148 bp With 182 enzymes: SgfI * March 28, 2012 13:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3283 3283 Size: 6148 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3958 278 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3491 1937 Size: 1554 4594 Acc65I G'GTAC_C Cuts at: 2066 3469 2066 Size: 1403 4745 AclI AA'CG_TT Cuts at: 1563 4275 4648 1563 Size: 2712 373 3063 Fragments arranged by size: 3063 2712 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3296 4211 5595 5863 1632 Size: 1664 915 1384 268 1917 Fragments arranged by size: 1917 1664 1384 915 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5951 AflIII A'CryG_T Cuts at: 1203 3099 5047 6024 1203 Size: 1896 1948 977 1327 Fragments arranged by size: 1948 1896 1327 977 AgeI A'CCGG_T Cuts at: 2809 4979 2809 Size: 2170 3978 AhdI GACnn_n'nnGTC Cuts at: 4877 4877 Size: 6148 AleI CACnn'nnGTG Cuts at: 1166 3104 1166 Size: 1938 4210 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6148 AlwNI CAG_nnn'CTG Cuts at: 3891 5463 3891 Size: 1572 4576 ApaLI G'TGCA_C Cuts at: 4205 5361 4205 Size: 1156 4992 ApoI r'AATT_y Cuts at: 1493 2663 3457 3683 3835 5944 1493 Size: 1170 794 226 152 2109 1697 Fragments arranged by size: 2109 1697 1170 794 226 152 AseI AT'TA_AT Cuts at: 160 1032 4702 160 Size: 872 3670 1606 Fragments arranged by size: 3670 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 AvaI C'yCGr_G Cuts at: 1870 1943 3351 3473 1870 Size: 73 1408 122 4545 Fragments arranged by size: 4545 1408 122 73 BamHI G'GATC_C Cuts at: 3478 3845 3478 Size: 367 5781 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2066 3469 4924 618 Size: 325 217 627 279 1403 1455 1842 Fragments arranged by size: 1842 1455 1403 627 325 279 217 BanII G_rGCy'C Cuts at: 1391 2754 3001 3254 3360 3414 3446 3467 1391 Size: 1363 247 253 106 54 32 21 4072 Fragments arranged by size: 4072 1363 253 247 106 54 32 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5177 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4339 4373 1903 Size: 34 2402 34 3678 Fragments arranged by size: 3678 2402 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4040 5250 4040 Size: 1210 4938 BclI T'GATC_A Cuts at: 1548 1548 Size: 6148 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2221 4759 136 Size: 107 122 71 631 605 549 2538 1525 Fragments arranged by size: 2538 1525 631 605 549 122 107 71 BglII A'GATC_T Cuts at: 2319 2688 6143 2319 Size: 369 3455 2324 Fragments arranged by size: 3455 2324 369 BlpI GC'TnA_GC Cuts at: 3565 3565 Size: 6148 Bme1580I G_kGCm'C Cuts at: 1790 4209 5365 1790 Size: 2419 1156 2573 Fragments arranged by size: 2573 2419 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6148 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2285 3879 4837 Size: 416 53 83 399 24 868 1594 958 Cuts at: 4837 442 Size: 1753 Fragments arranged by size: 1753 1594 958 868 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6148 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3321 3321 Size: 6148 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2177 2243 2429 2741 4808 1239 Size: 938 66 186 312 2067 2579 Fragments arranged by size: 2579 2067 938 312 186 66 Bpu10I CC'TnA_GC Cuts at: 3440 3440 Size: 6148 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2668 4273 5138 5436 2668 Size: 1605 865 298 3380 Fragments arranged by size: 3380 1605 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4811 882 Size: 617 3312 2219 Fragments arranged by size: 3312 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3102 3939 493 Size: 711 1898 837 2702 Fragments arranged by size: 2702 1898 837 711 BsaBI GATnn'nnATC Cuts at: 6142 6142 Size: 6148 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3955 4337 4972 275 Size: 53 83 186 936 2422 382 635 1451 Fragments arranged by size: 2422 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2809 4581 4979 5253 5400 5811 2809 Size: 1772 398 274 147 411 3146 Fragments arranged by size: 3146 1772 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3194 3224 1178 Size: 30 165 30 1791 30 4102 Fragments arranged by size: 4102 1791 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2174 2303 2492 2738 2963 3215 1186 Size: 988 129 189 246 225 252 4119 Fragments arranged by size: 4119 988 252 246 225 189 129 BseYI C'CCAG_C Cuts at: 2375 2681 2830 2856 3039 3324 5351 5847 2375 Size: 306 149 26 183 285 2027 496 2676 Fragments arranged by size: 2676 2027 496 306 285 183 149 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3124 3188 1163 Size: 573 1388 64 4123 Fragments arranged by size: 4123 1388 573 64 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3598 4359 5387 1540 Size: 275 186 1597 761 1028 2301 Fragments arranged by size: 2301 1597 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2310 2385 2707 2754 3001 3446 4209 4294 5365 Size: 75 322 47 247 445 763 85 1071 Cuts at: 5365 2310 Size: 3093 Fragments arranged by size: 3093 1071 763 445 322 247 85 75 47 BsmI GAATG_Cn' Cuts at: 3659 3752 3659 Size: 93 6055 BsmBI CGTCTCn'nnnn_ Cuts at: 3244 3244 Size: 6148 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2310 2385 2707 2754 3001 3254 3360 Size: 399 520 75 322 47 247 253 106 Cuts at: 3360 3414 3446 3467 4209 4294 5365 1391 Size: 54 32 21 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 520 399 322 253 247 106 85 75 54 47 32 21 BspEI T'CCGG_A Cuts at: 5811 5811 Size: 6148 BspHI T'CATG_A Cuts at: 4037 5767 4037 Size: 1730 4418 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3283 3503 844 Size: 2439 220 3489 Fragments arranged by size: 3489 2439 220 BsrBI CCG'CTC Cuts at: 1394 1823 4035 1394 Size: 429 2212 3507 Fragments arranged by size: 3507 2212 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4643 4817 65 Size: 1183 3395 174 1396 Fragments arranged by size: 3395 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2080 2694 2809 4792 4979 1700 Size: 110 270 614 115 1983 187 2869 Fragments arranged by size: 2869 1983 614 270 187 115 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6148 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6148 BssSI C'ACGA_G Cuts at: 4208 5220 4208 Size: 1012 5136 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6148 BstBI TT'CG_AA Cuts at: 4952 4952 Size: 6148 BstEII G'GTnAC_C Cuts at: 1184 3328 1184 Size: 2144 4004 BstYI r'GATC_y Cuts at: 1972 2319 2688 3478 3845 4230 4247 5699 5785 Size: 347 369 790 367 385 17 1452 86 Cuts at: 5785 5797 6143 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 790 385 369 367 347 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2072 2579 2072 Size: 507 5641 BtgI C'CryG_G Cuts at: 513 1065 2004 3127 3144 513 Size: 552 939 1123 17 3517 Fragments arranged by size: 3517 1123 939 552 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5409 Fragments arranged by size: 5409 683 56 BtsI GCAGTG_nn' Cuts at: 3372 3664 4458 4478 3372 Size: 292 794 20 5042 Fragments arranged by size: 5042 794 292 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6148 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6113 DraI TTT'AAA Cuts at: 3452 3452 Size: 6148 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2536 5155 809 Size: 507 1220 2619 1802 Fragments arranged by size: 2619 1802 1220 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2098 3194 3595 Size: 54 1425 50 275 18 268 1096 401 Cuts at: 3595 4484 6113 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1096 889 401 275 268 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3595 1537 Size: 275 1783 4090 Fragments arranged by size: 4090 1783 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4078 1353 Size: 2725 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4735 5109 5255 387 Size: 1064 138 3146 374 146 1280 Fragments arranged by size: 3146 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2752 2999 3444 2752 Size: 247 445 5456 Fragments arranged by size: 5456 445 247 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2177 2243 2429 2741 3296 4211 4808 Size: 393 545 66 186 312 555 915 597 Cuts at: 4808 5595 5863 1239 Size: 787 268 1524 Fragments arranged by size: 1524 915 787 597 555 545 393 312 268 186 66 EcoNI CCTnn'n_nnAGG Cuts at: 2531 3385 2531 Size: 854 5294 EcoO109I rG'GnC_Cy Cuts at: 2863 2863 Size: 6148 EcoRI G'AATT_C Cuts at: 3457 3835 3457 Size: 378 5770 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2341 2992 2341 Size: 651 5497 FspI TGC'GCA Cuts at: 4654 4654 Size: 6148 HaeII r_GCGC'y Cuts at: 1678 2257 5295 1678 Size: 579 3038 2531 Fragments arranged by size: 3038 2531 579 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2304 2336 3345 3377 4789 Size: 32 135 32 732 32 1009 32 1412 Cuts at: 4789 4821 4863 4895 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1412 1009 732 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2047 3492 3738 669 Size: 988 281 109 1445 246 3079 Fragments arranged by size: 3079 1445 988 281 246 109 HindIII A'AGCT_T Cuts at: 748 748 Size: 6148 HpaI GTT'AAC Cuts at: 3738 3738 Size: 6148 KpnI G_GTAC'C Cuts at: 2070 3473 2070 Size: 1403 4745 MfeI C'AATT_G Cuts at: 2621 3747 2621 Size: 1126 5022 MluI A'CGCG_T Cuts at: 6024 6024 Size: 6148 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2898 5262 5446 1305 Size: 1593 2364 184 2007 Fragments arranged by size: 2364 2007 1593 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4326 Fragments arranged by size: 4326 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3104 4106 4465 4624 518 Size: 648 1938 1002 359 159 2042 Fragments arranged by size: 2042 1938 1002 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2227 2263 2420 2872 3138 3172 3564 4241 Size: 621 36 157 452 266 34 392 677 Cuts at: 4241 5389 5634 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 621 452 392 266 245 157 36 34 NcoI C'CATG_G Cuts at: 513 1065 2004 3127 513 Size: 552 939 1123 3534 Fragments arranged by size: 3534 1123 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6148 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6148 NotI GC'GGCC_GC Cuts at: 3595 3595 Size: 6148 NspI r_CATG'y Cuts at: 1132 1229 3506 5051 1132 Size: 97 2277 1545 2229 Fragments arranged by size: 2277 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6148 PciI A'CATG_T Cuts at: 5047 5047 Size: 6148 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6148 PfoI T'CCnGG_A Cuts at: 2406 2406 Size: 6148 PmeI GTTT'AAAC Cuts at: 3452 3452 Size: 6148 PmlI CAC'GTG Cuts at: 3102 3102 Size: 6148 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4217 4249 5730 5762 4217 Size: 32 1481 32 4603 Fragments arranged by size: 4603 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6148 PsiI TTA'TAA Cuts at: 3718 3718 Size: 6148 PstI C_TGCA'G Cuts at: 830 2877 3500 830 Size: 2047 623 3478 Fragments arranged by size: 3478 2047 623 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6148 PvuII CAG'CTG Cuts at: 2227 2263 3138 2227 Size: 36 875 5237 Fragments arranged by size: 5237 875 36 SacI G_AGCT'C Cuts at: 2754 3001 3446 2754 Size: 247 445 5456 Fragments arranged by size: 5456 445 247 SalI G'TCGA_C Cuts at: 1936 3490 1936 Size: 1554 4594 SbfI CC_TGCA'GG Cuts at: 3500 3500 Size: 6148 ScaI AGT'ACT Cuts at: 4396 6108 4396 Size: 1712 4436 SfcI C'TryA_G Cuts at: 826 1045 2428 2873 3496 4631 5312 5503 826 Size: 219 1383 445 623 1135 681 191 1471 Fragments arranged by size: 1471 1383 1135 681 623 445 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6148 SmaI CCC'GGG Cuts at: 1872 3475 1872 Size: 1603 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2647 4252 5153 5415 820 Size: 197 926 704 1605 901 262 1553 Fragments arranged by size: 1605 1553 926 901 704 262 197 SnaBI TAC'GTA Cuts at: 493 3939 493 Size: 3446 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6148 SphI G_CATG'C Cuts at: 1132 3506 1132 Size: 2374 3774 SspI AAT'ATT Cuts at: 5 52 4072 5 Size: 47 4020 2081 Fragments arranged by size: 4020 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6148 StyI C'CwwG_G Cuts at: 513 1065 2004 3127 3587 513 Size: 552 939 1123 460 3074 Fragments arranged by size: 3074 1123 939 552 460 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3094 3316 4175 4192 4345 4530 4975 Size: 1255 1514 222 859 17 153 185 445 Cuts at: 4975 325 Size: 1498 Fragments arranged by size: 1514 1498 1255 859 445 222 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4394 6106 96 Size: 274 80 33 51 1443 2417 1712 138 Fragments arranged by size: 2417 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3194 3226 6108 6140 3194 Size: 32 2882 32 3202 Fragments arranged by size: 3202 2882 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6148 XbaI T'CTAG_A Cuts at: 2590 2944 3484 2590 Size: 354 540 5254 Fragments arranged by size: 5254 540 354 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2224 2278 2404 3064 2224 Size: 54 126 660 5308 Fragments arranged by size: 5308 660 126 54 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6148 XmaI C'CCGG_G Cuts at: 1870 3473 1870 Size: 1603 4545 XmnI GAAnn'nnTTC Cuts at: 4277 4277 Size: 6148 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3956 276 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstXI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI