(Linear) (Six-base) MAPSORT of: KIEE4110.seq Check: 7829 from: 1 to: 1443 KIEE4110.orf With 182 enzymes: SgfI * March 22, 2012 17:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1278 1443 Size: 1278 165 Acc65I G'GTAC_C Cuts at: 0 61 1443 Size: 61 1382 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1291 1443 Size: 1291 152 AflIII A'CryG_T Cuts at: 0 1094 1443 Size: 1094 349 AgeI A'CCGG_T Cuts at: 0 804 1443 Size: 804 639 AleI CACnn'nnGTG Cuts at: 0 1099 1443 Size: 1099 344 ApoI r'AATT_y Cuts at: 0 658 1443 Size: 658 785 AvaI C'yCGr_G Cuts at: 0 1346 1443 Size: 1346 97 BanI G'GyrC_C Cuts at: 0 61 1443 Size: 61 1382 BanII G_rGCy'C Cuts at: 0 749 996 1249 1355 1409 1441 1443 Size: 749 247 253 106 54 32 2 Fragments arranged by size: 749 253 247 106 54 32 2 BglI GCCn_nnn'nGGC Cuts at: 0 216 1443 Size: 216 1227 BglII A'GATC_T Cuts at: 0 314 683 1443 Size: 314 369 760 Fragments arranged by size: 760 369 314 BmrI ACTGGGnnnn_n' Cuts at: 0 280 1443 Size: 280 1163 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1316 1443 Size: 1316 127 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 172 238 424 736 1443 Size: 172 66 186 312 707 Fragments arranged by size: 707 312 186 172 66 Bpu10I CC'TnA_GC Cuts at: 0 1435 1443 Size: 1435 8 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 663 1443 Size: 663 780 BsaAI yAC'GTr Cuts at: 0 1097 1443 Size: 1097 346 BsaWI w'CCGG_w Cuts at: 0 804 1443 Size: 804 639 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1189 1219 1443 Size: 1189 30 224 Fragments arranged by size: 1189 224 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 169 298 487 733 958 1210 1443 Size: 169 129 189 246 225 252 233 Fragments arranged by size: 252 246 233 225 189 169 129 BseYI C'CCAG_C Cuts at: 0 370 676 825 851 1034 1319 1443 Size: 370 306 149 26 183 285 124 Fragments arranged by size: 370 306 285 183 149 124 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1119 1183 1443 Size: 1119 64 260 Fragments arranged by size: 1119 260 64 BsiHKAI G_wGCw'C Cuts at: 0 305 380 702 749 996 1441 1443 Size: 305 75 322 47 247 445 2 Fragments arranged by size: 445 322 305 247 75 47 2 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1239 1443 Size: 1239 204 Bsp1286I G_dGCh'C Cuts at: 0 305 380 702 749 996 1249 1355 1409 Size: 305 75 322 47 247 253 106 54 Cuts at: 1409 1441 1443 Size: 32 2 Fragments arranged by size: 322 305 253 247 106 75 54 47 32 2 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1278 1443 Size: 1278 165 BsrFI r'CCGG_y Cuts at: 0 75 689 804 1443 Size: 75 614 115 639 Fragments arranged by size: 639 614 115 75 BstEII G'GTnAC_C Cuts at: 0 1323 1443 Size: 1323 120 BstYI r'GATC_y Cuts at: 0 314 683 1443 Size: 314 369 760 Fragments arranged by size: 760 369 314 Bsu36I CC'TnA_GG Cuts at: 0 67 574 1443 Size: 67 507 869 Fragments arranged by size: 869 507 67 BtgI C'CryG_G Cuts at: 0 1122 1139 1443 Size: 1122 17 304 Fragments arranged by size: 1122 304 17 BtsI GCAGTG_nn' Cuts at: 0 1367 1443 Size: 1367 76 DrdI GACnn_nn'nnGTC Cuts at: 0 531 1443 Size: 531 912 EaeI y'GGCC_r Cuts at: 0 93 1189 1443 Size: 93 1096 254 Fragments arranged by size: 1096 254 93 EcoICRI GAG'CTC Cuts at: 0 747 994 1439 1443 Size: 747 247 445 4 Fragments arranged by size: 747 445 247 4 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 172 238 424 736 1291 1443 Size: 172 66 186 312 555 152 Fragments arranged by size: 555 312 186 172 152 66 EcoNI CCTnn'n_nnAGG Cuts at: 0 526 1380 1443 Size: 526 854 63 Fragments arranged by size: 854 526 63 EcoO109I rG'GnC_Cy Cuts at: 0 858 1443 Size: 858 585 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 336 987 1443 Size: 336 651 456 Fragments arranged by size: 651 456 336 HaeII r_GCGC'y Cuts at: 0 252 1443 Size: 252 1191 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 299 331 1340 1372 1443 Size: 299 32 1009 32 71 Fragments arranged by size: 1009 299 71 32 32 HincII GTy'rAC Cuts at: 0 42 1443 Size: 42 1401 KpnI G_GTAC'C Cuts at: 0 65 1443 Size: 65 1378 MfeI C'AATT_G Cuts at: 0 616 1443 Size: 616 827 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 893 1443 Size: 893 550 MslI CAynn'nnrTG Cuts at: 0 1099 1443 Size: 1099 344 MspA1I CmG'CkG Cuts at: 0 222 258 415 867 1133 1167 1443 Size: 222 36 157 452 266 34 276 Fragments arranged by size: 452 276 266 222 157 36 34 NcoI C'CATG_G Cuts at: 0 1122 1443 Size: 1122 321 PfoI T'CCnGG_A Cuts at: 0 401 1443 Size: 401 1042 PmlI CAC'GTG Cuts at: 0 1097 1443 Size: 1097 346 PstI C_TGCA'G Cuts at: 0 872 1443 Size: 872 571 PvuII CAG'CTG Cuts at: 0 222 258 1133 1443 Size: 222 36 875 310 Fragments arranged by size: 875 310 222 36 SacI G_AGCT'C Cuts at: 0 749 996 1441 1443 Size: 749 247 445 2 Fragments arranged by size: 749 445 247 2 SfcI C'TryA_G Cuts at: 0 423 868 1443 Size: 423 445 575 Fragments arranged by size: 575 445 423 SmlI C'TyrA_G Cuts at: 0 642 1443 Size: 642 801 StyI C'CwwG_G Cuts at: 0 1122 1443 Size: 1122 321 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1089 1311 1443 Size: 1089 222 132 Fragments arranged by size: 1089 222 132 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1189 1221 1443 Size: 1189 32 222 Fragments arranged by size: 1189 222 32 XbaI T'CTAG_A Cuts at: 0 585 939 1443 Size: 585 354 504 Fragments arranged by size: 585 504 354 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 219 273 399 1059 1443 Size: 219 54 126 660 384 Fragments arranged by size: 660 384 219 126 54 Enzymes that do cut: AarI Acc65I AcuI AflIII AgeI AleI ApoI AvaI BanI BanII BglI BglII BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BstEII BstYI Bsu36I BtgI BtsI DrdI EaeI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KpnI MfeI MmeI MslI MspA1I NcoI PfoI PmlI PstI PvuII SacI SfcI SmlI StyI TaqII TstI XbaI XcmI Enzymes that do not cut: AatII AccI AclI AfeI AflII AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BlpI Bme1580I BmgBI BmtI BsaI BsaBI BsaHI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII EagI EarI EciI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XhoI XmaI XmnI ZraI