################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaWI BseAI BseRI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrFI BsrGI BssHII BssSI BstBI BstUI Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoT22I FauI FbaI FseI FspI HapII HincII HindII HpaI HpaII KasI KpnI KspI MaeI MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 328 GTATAC GT^[AC][GT]AC AcsI 14 AAATTT [GA]^AATT[CT] AcyI 145 GGCGTC G[GA]^CG[CT]C AfaI 540 GTAC GT^AC AflIII 648 ACATGT A^C[GA][CT]GT AlwNI 211 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 827 GGGCCC GGGCC^C ApoI 14 AAATTT [GA]^AATT[CT] AspHI 1111 GAGCAC G[AT]GC[AT]^C BanI 846 GGCACC G^G[CT][GA]CC BbsI 286 GTCTTC GAAGAC(2/6) BglI 235 GCCCCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 800 AGATCT A^GATCT BpuAI 286 GTCTTC GAAGAC(2/6) BsaHI 145 GGCGTC G[GA]^CG[CT]C BsaI 252 GGTCTC GGTCTC(1/5) BsiHKAI 1111 GAGCAC G[AT]GC[AT]^C BsmBI 163 GAGACG CGTCTC(1/5) BsmFI 204 GGGAC GGGAC(10/14) BspLU11I 648 ACATGT A^CATGT BsrBI 508 GAGCGG CCGCTC(-3/-3) BsrDI 231 CATTGC GCAATG(2/0) BsrI 641 ACTGG ACTGG(1/-1) Bst1107I 328 GTATAC GTA^TAC BstAPI 210 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 100 GGTGACC G^GT[ACGT]ACC BstPI 100 GGTGACC G^GT[ACGT]ACC BstXI 30 CCAGAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 800 AGATCT [GA]^GATC[CT] BstZ17I 328 GTATAC GTA^TAC CfoI 923 GCGC GCG^C EcoO65I 100 GGTGACC G^GT[ACGT]ACC EcoRV 66 GATATC GAT^ATC EcoT14I 239 CCAAGG C^C[AT][AT]GG HaeII 922 AGCGCC [GA]GCGC^[CT] HgaI 146 GCGTC GACGC(5/10) HhaI 923 GCGC GCG^C Hin1I 145 GGCGTC G[GA]^CG[CT]C HinP1I 923 GCGC G^CGC HindIII 676 AAGCTT A^AGCTT MaeII 356 ACGT A^CGT MflI 800 AGATCT [GA]^GATC[CT] MseI 892 TTAA T^TAA MspA1I 1046 CAGCTG C[AC]G^C[GT]G NdeI 181 CATATG CA^TATG PvuII 1046 CAGCTG CAG^CTG RsaI 540 GTAC GT^AC SapI 687 GCTCTTC GCTCTTC(1/4) StyI 239 CCAAGG C^C[AT][AT]GG Tru9I 892 TTAA T^TAA XcmI 239 CCAAGGCTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 800 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### BalI 446 TGGCCA TGG^CCA 494 TGGCCA BanII 685 GGGCTC G[GA]GC[CT]^C 827 GGGCCC BsgI 579 CTGCAC GTGCAG(16/14) 796 GTGCAG BstF5I 333 CATCC GGATG(2/0) 40 GGATG DpnII 360 GATC ^GATC 801 GATC EaeI 446 TGGCCA [CT]^GGCC[GA] 494 TGGCCA EarI 526 CTCTTC CTCTTC(1/4) 688 CTCTTC FokI 333 CATCC GGATG(9/13) 40 GGATG Ksp632I 526 CTCTTC CTCTTC(1/4) 688 CTCTTC MboI 360 GATC ^GATC 801 GATC MluNI 446 TGGCCA TGG^CCA 494 TGGCCA MscI 446 TGGCCA TGG^CCA 494 TGGCCA NdeII 360 GATC ^GATC 801 GATC Sau3AI 360 GATC ^GATC 801 GATC SexAI 125 ACCTGGT A^CC[AT]GGT 341 ACCTGGT Tsp509I 15 AATT ^AATT 894 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AluI BbvI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BssKI BstNI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI HaeIII HinfI HphI ItaI MaeIII MboII MnlI MslI MvaI MwoI NlaIII NlaIV NspI PleI Sau96I ScrFI SfaNI TaqI TfiI TseI Tsp45I TspRI TthHB8I