################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbsI BcgI BcnI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MflI MluI MroI MspA1I MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspI StuI SwaI TaqI TfiI Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AcsI 25 GAATTT [GA]^AATT[CT] AlwI 148 GATCC GGATC(4/5) ApaI 618 GGGCCC GGGCC^C ApoI 25 GAATTT [GA]^AATT[CT] BalI 425 TGGCCA TGG^CCA BanII 618 GGGCCC G[GA]GC[CT]^C BbvCI 406 CCTCAGC CCTCAGC(-5/-2) BciVI 512 GTATCC GTATCC(6/5) BclI 908 TGATCA T^GATCA BfaI 152 CTAG C^TAG BglI 340 GCCGTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 618 GGGCCC G[AGT]GC[ACT]^C BsaWI 809 ACCGGA [AT]^CCGG[AT] BseRI 31 CTCCTC GAGGAG(10/8) BsgI 466 CTGCAC GTGCAG(16/14) BsmFI 604 GGGAC GGGAC(10/14) Bsp1286I 618 GGGCCC G[AGT]GC[ACT]^C Bsp1407I 827 TGTACA T^GTACA BspHI 398 TCATGA T^CATGA BsrDI 542 CATTGC GCAATG(2/0) BsrGI 827 TGTACA T^GTACA BsrI 112 ACTGG ACTGG(1/-1) DsaI 614 CCATGG C^C[GA][CT]GG EaeI 425 TGGCCA [CT]^GGCC[GA] EcoRV 202 GATATC GAT^ATC EcoT22I 282 ATGCAT ATGCA^T FbaI 908 TGATCA T^GATCA HapII 810 CCGG C^CGG HpaII 810 CCGG C^CGG MaeI 152 CTAG C^TAG MfeI 608 CAATTG C^AATTG MluNI 425 TGGCCA TGG^CCA MscI 425 TGGCCA TGG^CCA MslI 102 CATCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 810 CCGG C^CGG MunI 608 CAATTG C^AATTG NcoI 614 CCATGG C^CATGG NsiI 282 ATGCAT ATGCA^T NspI 692 GCATGC [GA]CATG^[CT] RcaI 398 TCATGA T^CATGA ScaI 592 AGTACT AGT^ACT SfaNI 281 GATGC GCATC(5/9) SphI 692 GCATGC GCATG^C SspBI 827 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AvaII 801 GGACC G^G[AT]CC 807 GGACC BsaBI 135 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 423 GATGGCCATC BstF5I 778 CATCC GGATG(2/0) 280 GGATG DraII 618 GGGCCCC [GA]G^G[ACGT]CC[CT] 892 GGGGCCT EarI 73 CTCTTC CTCTTC(1/4) 667 CTCTTC EcoO109I 618 GGGCCCC [GA]G^G[ACGT]CC[CT] 892 GGGGCCT EcoT14I 614 CCATGG C^C[AT][AT]GG 896 CCTTGG FokI 778 CATCC GGATG(9/13) 280 GGATG HinfI 155 GACTC G^A[ACGT]TC 325 GAGTC Ksp632I 73 CTCTTC CTCTTC(1/4) 667 CTCTTC MamI 135 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 423 GATGGCCATC MseI 536 TTAA T^TAA 575 TTAA MwoI 228 GCCAAAAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 340 GCCGTGATGGC PflMI 306 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 726 CCACTTCATGG PleI 155 GACTC GAGTC(4/5) 325 GAGTC PstI 61 CTGCAG CTGCA^G 238 CTGCAG SfcI 61 CTGCAG C^T[GA][CT]AG 238 CTGCAG StyI 614 CCATGG C^C[AT][AT]GG 896 CCTTGG Tru9I 536 TTAA T^TAA 575 TTAA Van91I 306 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 726 CCACTTCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AfaI AluI BbvI BsaJI BsiYI BslI BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HphI ItaI MaeIII MboI MboII MnlI MvaI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TseI Tsp45I Tsp509I TspRI