(Circular) (Six-base) MAPSORT of: pFN21AE5386.seq Check: 3704 from: 1 to: 5626 pFN21AE5386 5626 bp With 182 enzymes: SgfI * January 30, 2014 17:18 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3436 278 Size: 53 83 186 936 1900 2468 Fragments arranged by size: 2468 1900 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2969 1937 Size: 1032 4594 Acc65I G'GTAC_C Cuts at: 2947 2947 Size: 5626 AclI AA'CG_TT Cuts at: 1563 3753 4126 1563 Size: 2190 373 3063 Fragments arranged by size: 3063 2190 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3689 5073 5341 1632 Size: 2057 1384 268 1917 Fragments arranged by size: 2057 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5429 AflIII A'CryG_T Cuts at: 1203 4525 5502 1203 Size: 3322 977 1327 Fragments arranged by size: 3322 1327 977 AgeI A'CCGG_T Cuts at: 4457 4457 Size: 5626 AhdI GACnn_n'nnGTC Cuts at: 4355 4355 Size: 5626 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5626 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5626 AlwNI CAG_nnn'CTG Cuts at: 3369 4941 3369 Size: 1572 4054 ApaI G_GGCC'C Cuts at: 2627 2627 Size: 5626 ApaLI G'TGCA_C Cuts at: 3683 4839 3683 Size: 1156 4470 ApoI r'AATT_y Cuts at: 1493 2030 2935 3161 3313 5422 1493 Size: 537 905 226 152 2109 1697 Fragments arranged by size: 2109 1697 905 537 226 152 AseI AT'TA_AT Cuts at: 160 1032 4180 160 Size: 872 3148 1606 Fragments arranged by size: 3148 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5626 AvaI C'yCGr_G Cuts at: 1870 1943 2951 1870 Size: 73 1008 4545 Fragments arranged by size: 4545 1008 73 BamHI G'GATC_C Cuts at: 2956 3323 2956 Size: 367 5259 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2947 4402 618 Size: 325 217 627 1160 1455 1842 Fragments arranged by size: 1842 1455 1160 627 325 217 BanII G_rGCy'C Cuts at: 1391 2627 2945 1391 Size: 1236 318 4072 Fragments arranged by size: 4072 1236 318 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4655 BbvCI CC'TCA_GC Cuts at: 2412 2412 Size: 5626 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3817 3851 1903 Size: 34 50 34 1796 34 3678 Fragments arranged by size: 3678 1796 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2528 3518 4728 2528 Size: 990 1210 3426 Fragments arranged by size: 3426 1210 990 BclI T'GATC_A Cuts at: 1548 2913 1548 Size: 1365 4261 BfrBI ATG'CAT Cuts at: 2289 2289 Size: 5626 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2351 4237 136 Size: 107 122 71 631 605 679 1886 1525 Fragments arranged by size: 1886 1525 679 631 605 122 107 71 BglII A'GATC_T Cuts at: 5621 5621 Size: 5626 BlpI GC'TnA_GC Cuts at: 3043 3043 Size: 5626 Bme1580I G_kGCm'C Cuts at: 1790 2627 3687 4843 1790 Size: 837 1060 1156 2573 Fragments arranged by size: 2573 1156 1060 837 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5626 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2127 3357 4315 Size: 416 53 83 399 24 710 1230 958 Cuts at: 4315 442 Size: 1753 Fragments arranged by size: 1753 1230 958 710 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5626 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4286 1239 Size: 3047 2579 Bpu10I CC'TnA_GC Cuts at: 2412 2412 Size: 5626 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3751 4616 4914 3751 Size: 865 298 4463 Fragments arranged by size: 4463 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4289 882 Size: 617 2790 2219 Fragments arranged by size: 2790 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3417 493 Size: 711 2213 2702 Fragments arranged by size: 2702 2213 711 BsaBI GATnn'nnATC Cuts at: 2144 2432 5620 2144 Size: 288 3188 2150 Fragments arranged by size: 3188 2150 288 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3433 3815 4450 275 Size: 53 83 186 936 1900 382 635 1451 Fragments arranged by size: 1900 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2814 4059 4457 4731 4878 5289 2814 Size: 1245 398 274 147 411 3151 Fragments arranged by size: 3151 1245 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2321 2351 1178 Size: 30 165 30 918 30 4453 Fragments arranged by size: 4453 918 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 1186 Size: 841 4785 BseYI C'CCAG_C Cuts at: 4829 5325 4829 Size: 496 5130 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2456 1163 Size: 573 720 4333 Fragments arranged by size: 4333 720 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3076 3837 4865 1540 Size: 275 186 1075 761 1028 2301 Fragments arranged by size: 2301 1075 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3687 3772 4843 3687 Size: 85 1071 4470 Fragments arranged by size: 4470 1071 85 BsmI GAATG_Cn' Cuts at: 3137 3230 3137 Size: 93 5533 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2627 2945 3687 3772 4843 1391 Size: 399 837 318 742 85 1071 2174 Fragments arranged by size: 2174 1071 837 742 399 318 85 BspEI T'CCGG_A Cuts at: 5289 5289 Size: 5626 BspHI T'CATG_A Cuts at: 2403 3515 5245 2403 Size: 1112 1730 2784 Fragments arranged by size: 2784 1730 1112 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2981 844 Size: 2137 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3513 1394 Size: 429 1690 3507 Fragments arranged by size: 3507 1690 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2546 4121 4295 65 Size: 1183 1298 1575 174 1396 Fragments arranged by size: 1575 1396 1298 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4270 4457 1700 Size: 110 2460 187 2869 Fragments arranged by size: 2869 2460 187 110 BsrGI T'GTAC_A Cuts at: 96 2832 96 Size: 2736 2890 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5626 BssSI C'ACGA_G Cuts at: 3686 4698 3686 Size: 1012 4614 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5626 BstBI TT'CG_AA Cuts at: 4430 4430 Size: 5626 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5626 BstYI r'GATC_y Cuts at: 1972 2956 3323 3708 3725 5177 5263 5275 5621 Size: 984 367 385 17 1452 86 12 346 Cuts at: 5621 1972 Size: 1977 Fragments arranged by size: 1977 1452 984 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2619 513 Size: 552 1554 3520 Fragments arranged by size: 3520 1554 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4887 Fragments arranged by size: 4887 683 56 BtsI GCAGTG_nn' Cuts at: 2228 3142 3936 3956 2228 Size: 914 794 20 3898 Fragments arranged by size: 3898 914 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5626 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5591 DraI TTT'AAA Cuts at: 2930 2930 Size: 5626 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4633 809 Size: 507 3317 1802 Fragments arranged by size: 3317 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2430 3073 3962 Size: 54 1425 50 275 18 600 643 889 Cuts at: 3962 5591 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 643 600 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3073 1537 Size: 275 1261 4090 Fragments arranged by size: 4090 1261 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2084 2678 3556 1353 Size: 731 594 878 3423 Fragments arranged by size: 3423 878 731 594 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4213 4587 4733 387 Size: 1064 138 2624 374 146 1280 Fragments arranged by size: 2624 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3689 4286 5073 5341 1239 Size: 393 2057 597 787 268 1524 Fragments arranged by size: 2057 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2624 2898 2624 Size: 274 5352 EcoRI G'AATT_C Cuts at: 2935 3313 2935 Size: 378 5248 EcoRV GAT'ATC Cuts at: 2209 2209 Size: 5626 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2917 2917 Size: 5626 FspI TGC'GCA Cuts at: 4132 4132 Size: 5626 HaeII r_GCGC'y Cuts at: 1678 4773 1678 Size: 3095 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4267 4299 4341 4373 1373 Size: 32 135 32 2695 32 42 32 2626 Fragments arranged by size: 2695 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2970 3216 669 Size: 988 281 1032 246 3079 Fragments arranged by size: 3079 1032 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5626 HpaI GTT'AAC Cuts at: 3216 3216 Size: 5626 KpnI G_GTAC'C Cuts at: 2951 2951 Size: 5626 MfeI C'AATT_G Cuts at: 2613 3225 2613 Size: 612 5014 MluI A'CGCG_T Cuts at: 5502 5502 Size: 5626 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2535 4740 4924 1305 Size: 1230 2205 184 2007 Fragments arranged by size: 2205 2007 1230 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2432 10 Size: 54 1425 343 600 3204 Fragments arranged by size: 3204 1425 600 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2111 3584 3943 4102 518 Size: 648 945 1473 359 159 2042 Fragments arranged by size: 2042 1473 945 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3042 3719 4867 5112 1606 Size: 1436 677 1148 245 2120 Fragments arranged by size: 2120 1436 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2619 513 Size: 552 1554 3520 Fragments arranged by size: 3520 1554 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5626 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5626 NotI GC'GGCC_GC Cuts at: 3073 3073 Size: 5626 NsiI A_TGCA'T Cuts at: 2291 2291 Size: 5626 NspI r_CATG'y Cuts at: 1132 1229 2701 2984 4529 1132 Size: 97 1472 283 1545 2229 Fragments arranged by size: 2229 1545 1472 283 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5626 PciI A'CATG_T Cuts at: 4525 4525 Size: 5626 PflMI CCAn_nnn'nTGG Cuts at: 1108 2317 2737 1108 Size: 1209 420 3997 Fragments arranged by size: 3997 1209 420 PmeI GTTT'AAAC Cuts at: 2930 2930 Size: 5626 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2240 2272 3695 3727 5208 5240 2240 Size: 32 1423 32 1481 32 2626 Fragments arranged by size: 2626 1481 1423 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5626 PsiI TTA'TAA Cuts at: 3196 3196 Size: 5626 PspOMI G'GGCC_C Cuts at: 2623 2623 Size: 5626 PstI C_TGCA'G Cuts at: 830 2070 2247 2978 830 Size: 1240 177 731 3478 Fragments arranged by size: 3478 1240 731 177 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5626 SalI G'TCGA_C Cuts at: 1936 2968 1936 Size: 1032 4594 SbfI CC_TGCA'GG Cuts at: 2978 2978 Size: 5626 ScaI AGT'ACT Cuts at: 2599 3874 5586 2599 Size: 1275 1712 2639 Fragments arranged by size: 2639 1712 1275 SfcI C'TryA_G Cuts at: 826 1045 2066 2243 2974 4109 4790 4981 826 Size: 219 1021 177 731 1135 681 191 1471 Fragments arranged by size: 1471 1135 1021 731 681 219 191 177 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5626 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5626 SmaI CCC'GGG Cuts at: 1872 2953 1872 Size: 1081 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3730 4631 4893 820 Size: 197 926 1787 901 262 1553 Fragments arranged by size: 1787 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3417 493 Size: 2924 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5626 SphI G_CATG'C Cuts at: 1132 2701 2984 1132 Size: 1569 283 3774 Fragments arranged by size: 3774 1569 283 SspI AAT'ATT Cuts at: 5 52 3550 5 Size: 47 3498 2081 Fragments arranged by size: 3498 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5626 StyI C'CwwG_G Cuts at: 513 1065 2619 2901 3065 513 Size: 552 1554 282 164 3074 Fragments arranged by size: 3074 1554 552 282 164 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3653 3670 3823 4008 4453 325 Size: 1255 2073 17 153 185 445 1498 Fragments arranged by size: 2073 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2597 2832 2851 Size: 274 80 33 51 1443 620 235 19 Cuts at: 2851 3872 5584 96 Size: 1021 1712 138 Fragments arranged by size: 1712 1443 1021 620 274 235 138 80 51 33 19 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5586 5618 5586 Size: 32 5594 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5626 XbaI T'CTAG_A Cuts at: 2962 2962 Size: 5626 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5626 XmaI C'CCGG_G Cuts at: 1870 2951 1870 Size: 1081 4545 XmnI GAAnn'nnTTC Cuts at: 3755 3755 Size: 5626 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3434 276 Size: 53 83 186 936 1900 2468 Fragments arranged by size: 2468 1900 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI