################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BclI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KspI MaeII MfeI MluI MluNI MroI MscI MspA1I MunI NaeI NarI NdeI NgoMIV NheI NotI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 883 GGTACC G^GTACC AccII 190 CGCG CG^CG AciI 204 CCGC CCGC(-3/-1) AflII 127 CTTAAG C^TTAAG AseI 1449 ATTAAT AT^TAAT AsnI 1449 ATTAAT AT^TAAT Asp718I 883 GGTACC G^GTACC BanI 883 GGTACC G^G[CT][GA]CC BciVI 640 GGATAC GTATCC(6/5) BcnI 210 CCGGG CC^[GC]GG BfrI 127 CTTAAG C^TTAAG BglII 1155 AGATCT A^GATCT BsaBI 641 GATACATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 499 GAGACC GGTCTC(1/5) BsmFI 542 GGGAC GGGAC(10/14) BstUI 190 CGCG CG^CG BstXI 420 CCATGAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DsaI 90 CCATGG C^C[GA][CT]GG EarI 1385 GAAGAG CTCTTC(1/4) EcoNI 126 CCTTAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HapII 210 CCGG C^CGG HincII 1415 GTCAAC GT[CT]^[GA]AC HindII 1415 GTCAAC GT[CT]^[GA]AC HpaII 210 CCGG C^CGG KpnI 883 GGTACC GGTAC^C Ksp632I 1385 GAAGAG CTCTTC(1/4) MamI 641 GATACATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspI 210 CCGG C^CGG MvnI 190 CGCG CG^CG NciI 210 CCGGG CC^[GC]GG NcoI 90 CCATGG C^CATGG NlaIV 883 GGTACC GG[ACGT]^[ACGT]CC NruI 189 TCGCGA TCG^CGA NspI 999 ACATGC [GA]CATG^[CT] PflMI 340 CCAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 737 GACTCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1449 ATTAAT AT^TAAT SapI 1385 GAAGAGC GCTCTTC(1/4) ScaI 402 AGTACT AGT^ACT SspI 438 AATATT AAT^ATT StuI 317 AGGCCT AGG^CCT TaqI 1332 TCGA T^CGA TthHB8I 1332 TCGA T^CGA Van91I 340 CCAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### AlwI 243 GATCC GGATC(4/5) 572 GATCC AvaII 184 GGACC G^G[AT]CC 466 GGACC BseRI 352 GAGGAG GAGGAG(10/8) 828 GAGGAG BsmI 1369 GAATGC GAATGC(1/-1) 1222 GCATTC HaeIII 318 GGCC GG^CC 507 GGCC HindIII 1052 AAGCTT A^AGCTT 1137 AAGCTT MslI 421 CATGAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 958 CACCAATATG SmlI 127 CTTAAG C^T[CT][GA]AG 668 CTCAAG XcmI 171 CCAGGATTCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 575 CCATGCCAAAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AcsI AfaI AluI AlwNI ApoI Asp700I AspHI BanII BbvI BfaI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRI EcoRII EcoT14I Fnu4HI FokI HinfI HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII PleI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII XmnI